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Citations for Leica :
201 - 250 of 1635 citations for Perfluoro 2 oxo 3 6 dimethyl 1 4 dioxane since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: ... We cut 6 µm thick slices using a microtome (Biocut 2035, Leica Instruments GmbH), and mounted them on poly-L-lysine coated slides (Menzel-Glaser ...
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bioRxiv - Immunology 2022Quote: ... Cryosections (6 μm) of LNs were obtained using a CM1950 cryostat (Leica Microsystems, Germany). Tissue samples were captured on Superfrost plus microscope slides (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... antigen retrieval was performed by immersion in Epitope Retrieval Solution pH 6 (Leica Biosystems) and incubated at 60°C in an oven for 18 hours ...
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bioRxiv - Developmental Biology 2024Quote: ... and cut to 6 μm sections using a Leica CM 3050S cryostat (Leica Microsystems). For histology ...
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bioRxiv - Neuroscience 2020Quote: ... All mouse brain tissue specimens were fixed by immersion in 4% PFA overnight at 4 °C and sectioned at 50 μm using a vibratome (Leica).
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bioRxiv - Neuroscience 2022Quote: ... Brains were post-fixed overnight in 4% PFA at 4 °C and sectioned at 40 μm using a vibratome (VT1000S, Leica) except for experiments involving the RNAscope kit (ACD Bio.) ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... Brains were removed and post-fixed in 4 % PFA in PBS at 4 °C for 24 h and then sectioned using a vibratome (Leica) with thicknesses of 40 μm ...
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bioRxiv - Neuroscience 2024Quote: ... then fixed overnight at 4 °C in 4% PFA and coronally sectioned at 35 μm intervals using a freezing microtome (CM3050S, Leica). Brain sections were permeabilized with a solution containing 5% bovine serum albumin and 1% Triton X-100 in PBS for 40 minutes at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... The brain was then extracted and saved in 4% PFA overnight at 4 °C before being resected into 70 μm slices using a VT1000s vibratome (Leica Biosystems ...
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bioRxiv - Neuroscience 2022Quote: ... Brains were then post-fixed for overnight in 4% paraformaldehyde at 4 and sectioned into 40 µm sagittal slices using a vibratome (VT1000S, Leica). Brain sections were incubated in blocking solution (0.2% Triton X-100 in PBS with 5% normal goat serum ...
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bioRxiv - Physiology 2021Quote: Immunohistochemical staining in pancreas and fat sections was carried out on 1-2 μm section cut from paraffin blocks in an automated immunostainer from Leica biosystems (BOND RX ...
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bioRxiv - Immunology 2021Quote: ... Sections were serially imaged over the course of 1-2 hours and compiled using LAS X software (Leica Microsystems, USA). Image analysis was performed using Imaris software (Bitplane ...
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bioRxiv - Developmental Biology 2023Quote: ... dehydrated in methanol and cleared in Benzyl Alcohol/Benzyl Benzoate (1/2) prior to mounting and imaging with a SP8X confocal microscope (Leica). A 3D binary mask was built using Fiji to obtain a binary image of the tissue ...
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bioRxiv - Neuroscience 2023Quote: ... the animals were placed under 0.8-1% isoflurane anesthesia (2% used for induction) and imaged on an upright laser-scanning confocal (Leica SP8) or upright laser-scanning two-photon microscope (Bruker Ultima ...
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bioRxiv - Cancer Biology 2022Quote: ... with antigen retrieval at 100ºC for 20 min with Epitope Retrieval Solution 1 or 2 respectively (AR9961, AR9640, Leica Biosystems). TMAs were blind-scored by multiplying staining intensity with staining coverage ...
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bioRxiv - Cell Biology 2024Quote: ... and EB3 comets in single cells were imaged for 60 seconds (1 frame per 2 seconds) with a Leica DMi8 microscope (Leica). Only cells exhibiting both tdTomato and EYFP signals were included in the analysis ...
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bioRxiv - Microbiology 2022Quote: ... Then tissues were sectioned to 3 μm by microtome (Leica). Next ...
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bioRxiv - Immunology 2021Quote: ... 3 x H2O) on a linear stainer (Leica Biosystems, ST4020). Epitope retrieval was performed at 97C for 10 min with the Dako Target Retrieval Solution pH 9 (Agilent ...
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bioRxiv - Biophysics 2022Quote: ... The 3D model in Figure 3 was analyzed by Leica LAS X ...
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bioRxiv - Neuroscience 2021Quote: ... then sectioned coronally into 25-µm-thick slices (1:4 series of the PVN) using a Leica CM1900 cryostat (Leica Microsystems, Wetzlar, Germany). Sections were subjected to IHC using standard methods ...
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bioRxiv - Neuroscience 2021Quote: ... post-fixed in 4% PFA for 48 h at 4 °C and cut into 60 μm coronal sections using a vibratome (VT1200S, Leica, Germany). The sections were post-fixed (1 h) ...
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bioRxiv - Neuroscience 2023Quote: ... incubated in 4% paraformaldehyde overnight at 4°C, and sectioned coronally (thickness, 50–100 μm) using a vibratome (VT1200S, Leica Microsystems). Brain slices were incubated with 0.1% Triton X-100/phosphate-buffered saline for 10Cmin and blocked with Blocking-One P for 30 min ...
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bioRxiv - Neuroscience 2024Quote: ... post-fixed in 4% PFA for 48 h at 4°C and cut into 100 μm coronal sections using a vibratome (VT1200S, Leica, Germany). The sections were post-fixed (1 h) ...
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bioRxiv - Neuroscience 2024Quote: ... Extracted brains were then stored in 4% PFA overnight at 4 before being sliced at 60 µm using a vibratome (Leica VT1000S). PVN and rPa regions were identified via the Allen Brain Atlas ...
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bioRxiv - Neuroscience 2023Quote: ... Average power for excitation ranged from ∼35 mW to ∼80 mW measured at the front face of the objective (CA1 imaging: Nikon 16x, 3 mm WD, 0.8 NA; DG & CA3 imaging: Leica 25x, 3 mm WD, 1.0 NA). A 512 x 796 pixel field of view (FOV ...
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bioRxiv - Plant Biology 2020Quote: ... Thin sections (60-70 nm) were obtained with a Leica Ultracut UC 6 (Leica Microsystems), mounted on 150 mesh nickel grids ...
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bioRxiv - Cancer Biology 2022Quote: ... embedded tissues were sectioned into 6 um sections and loaded into BOND RXm (Leica Biosystems) for a series of stainings ...
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bioRxiv - Developmental Biology 2022Quote: ... 80 hpf and 6 dpf by visual inspection using a M165 FC fluorescence stereomicroscope (Leica). For F0 crispants analysis ...
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bioRxiv - Neuroscience 2021Quote: ... 300 µm for C57BL/6 mice) were cut using a VT1200s vibratome (Leica Microsystems Co.) in a sucrose-based cutting solution containing (in mM) ...
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bioRxiv - Bioengineering 2023Quote: ... The histological sections were taken at a thickness of 6 μm with a microtome (Leica RM2255 Microtome ...
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bioRxiv - Bioengineering 2022Quote: ... All samples were sectioned at a thickness of 6 μm using a rotary microtome (Leica). Sections were stained with H&E and 2% Alizarin Red solution (all Sigma-Aldrich).
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bioRxiv - Cell Biology 2024Quote: ... OCT samples were sectioned at 6 µm sections using a CM3050 cryostat (Leica Microsystems GmbH). For immunofluorescence or immunochemistry ...
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bioRxiv - Neuroscience 2024Quote: ... and 6 m/s velocity (Leica MyNeuroLab Impact One Stereotaxic Impactor, Leica Biosystems, Richmond, IL). To induce TBI ...
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bioRxiv - Cell Biology 2022Quote: ... pre-blotted and blotted with filter paper (Whatman 1) for 2 sec followed by plunging into liquid ethane using a Leica EM GP (Leica Microsystems). The samples were stored in liquid nitrogen until data acquisition ...
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bioRxiv - Cell Biology 2021Quote: ... and freeze substituted in anhydrous acetone containing 1 % OsO4/ 2% H2O for 64h using the Automatic Freeze Substitution unit (AFS; Leica Microsystems). The samples were included in Epon ...
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bioRxiv - Cell Biology 2022Quote: ... and freeze substituted in anhydrous acetone containing 1% OsO4/2% H2O for 64 h using the Automatic Freeze Substitution unit (AFS; Leica Microsystems). The samples were included in EMbed 812 ...
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bioRxiv - Neuroscience 2022Quote: ... mice were anesthetized with a mixture of oxygen (1 L/min) and isoflurane 2% (Baxter AG, Vienna, Austria) and placed in a stereotactic frame (Angle One; Leica, Germany). The skin was locally anesthetized with 40– 50 μL lidocaine 0.5% and disinfected with betadine.
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bioRxiv - Biochemistry 2023Quote: ... Frozen samples collected on metal carriers were transferred under liquid nitrogen into a pre-frozen cryotube containing 1 ml freeze-substitution solution (2% OsO4 in 100% acetone with 1% lecithin) and finally to a freeze substitution unit (Leica EMAFS) for processing ...
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bioRxiv - Plant Biology 2024Quote: ... 4 DAT seedlings were treated with 1 M PEP1 for 24h before they were imaged using confocal miscrscopes (Leica TCS SP8 and Zeiss LSM700). To visualize the GFP protein ...
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bioRxiv - Neuroscience 2020Quote: ... post-fixed in 4% PFA for 24 hours at 4°C and sectioned (20-60 μm) using a vibratome (Leica VT 1000s). If necessary ...
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bioRxiv - Neuroscience 2020Quote: ... Brains were extracted and kept in PFA 4% for at least 24h at 4°C and then cut in a fixed material vibratome (VT 1000S, Leica, Wetzlar, Germany) in 50 µm slices ...
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bioRxiv - Microbiology 2023Quote: ... and 2% lead-citrate solution (Leica Ultrostain II ...
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bioRxiv - Cell Biology 2023Quote: ... sectioned (4 um) using a cryostat CM1850 (Leica, CA), mounted onto microscope slides ...
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bioRxiv - Developmental Biology 2020Quote: ... at least 3 transverse slices cut with a vibratome (VT1000S, Leica) at different anterior-posterior levels representing anterior ...
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bioRxiv - Neuroscience 2023Quote: ... and sections (3 µm) were cut using a microtome (LEICA RM2255). The sections were deparaffinized by bathing twice in xylene (for 5 min each ...
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bioRxiv - Microbiology 2023Quote: ... Tissues were sectioned at 3 μm using a microtome (Leica, Germany) and the sections were stained with hematoxylin–eosin (HE ...
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bioRxiv - Systems Biology 2023Quote: ... we collected 3 10 µm sections using a Microtome (Leica Byostems). We then extracted RNA using Qiagen RNAse FFPE kit according to manufacturer instructions and evaluated the percentage of total RNA fragments longer than 200 nucleotides (DV200 ...
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bioRxiv - Neuroscience 2022Quote: ... Brains were removed from freshly euthanized mice and placed in 10% formalin at 4 °C for 24 h before embedding in paraffin blocks and preparing 4-μm tissue sections using a microtome (Leica Microsystems, Wetzlar, Germany). We detected MAGL mRNA in situ using the RNAscope 2.5 Assay for Ventana Discovery Ultra system (ACD Bio ...
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bioRxiv - Cell Biology 2020Quote: ... Serial thin sections (100 nm) were cut using a Leica UC 6 ultramicrotome (Leica, Wetzlar, Germany) from the surface of the block until approximately 4-5 microns in to ensure complete capture of the cell volumes ...
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bioRxiv - Developmental Biology 2021Quote: ... The dynamics of embryo spreading were examined for 6 hours under a Leica M165C (Leica, German) microscope coupled to a Leica DFC420 (5.0 MP ...