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Citations for Leica :
201 - 250 of 286 citations for Parvalbumin PVALB cDNA ORF Clone Mouse N His tag since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... Tissue was then incubated in primary antibody solution made with 1:250 mouse anti-Ki67 (Leica Biosystems), 1% NDS ...
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bioRxiv - Physiology 2024Quote: Z-stack images of mouse carotid arteries were taken using a confocal microscope (SP8; Leica, Wetzlar, Germany) and LAS AF lite software (Leica) ...
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bioRxiv - Neuroscience 2024Quote: One slide/mouse (with all 15 sections) was imaged using an epifluorescence microscope (Leica DMi8, Leica Microsystems) at 20X ...
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bioRxiv - Neuroscience 2024Quote: One slide/mouse (with all 15 sections) was imaged using an epifluorescence microscope (Leica DMi8, Leica Microsystems) at 20X ...
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bioRxiv - Neuroscience 2024Quote: ... Mouse brains were sectioned at 30 or 50 micron thickness on a cryostat (Leica Instruments GmbH, Germany). The sections were permeabilized with 0.3% Triton X-100 and non-specific binding sites were blocked by incubating sections in 5% normal donkey serum (D9663-10ML ...
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bioRxiv - Microbiology 2020Quote: ... followed by a polymer-labeled goat anti-mouse IgG coupled with alkaline phosphatase (30 minutes; Powervision, Leica Biosystems). Fast Red was used as the chromogen (15 minutes) ...
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bioRxiv - Developmental Biology 2022Quote: ... adult mouse brain tissue samples were embedded in 4% agarose and sections were cut using vibratome (Leica VT1200S). Until further processing sections were stored in ice-cold 1xPBS ...
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bioRxiv - Neuroscience 2020Quote: 300um thick coronal acute brain slices were prepared from P18-20 mouse using a virbrating microtome (Leica VT1200S) in ice cold cutting solution containing in mM (119 Choline Cl ...
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bioRxiv - Neuroscience 2022Quote: ... 300μm-thick coronal slices containing the SCN were obtained from PER2::LUC mouse brains using a vibratome (Leica) and placed on a semi-permeable membrane insert (PTFE ...
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bioRxiv - Neuroscience 2023Quote: ... Mouse somatosensory cortical slices (350 μm) were prepared in the coronal plane using a vibratome (Leica 1200 V) in cutting solution ...
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bioRxiv - Pathology 2023Quote: ... Mouse is then placed into a petri dish and onto the stage of an inverted microscope (Leica, DMi8). 4 videos of 90 sec were acquired per mice and the number of contraction and dilatation (difference between maximum and minimum diameter ...
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bioRxiv - Neuroscience 2022Quote: Human and mouse brain tissue was sectioned (30 μm-thick sections) using a SM2010 R sliding microtome (Leica) equipped with a BFS-3MP freezing stage and cooling unit (Physitemp ...
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bioRxiv - Neuroscience 2023Quote: ... surface vasculature map was draw on a notebook for each mouse under microscope with light source (Leica M50) and put ‘*’ marker near the location of FOVs in this map for reference when doing the first imaging session ...
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bioRxiv - Neuroscience 2023Quote: ... samples of mouse brain were cut into 100 µm thick sections using a vibratome (Leica VT1200S, Vienna, Austria). Sections containing relevant regions of interest were rinsed in 0.15 M cacodylate buffer containing 2 mM calcium chloride 3 times for 10 minutes each followed by a secondary fixation in 1% osmium tetroxide ...
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bioRxiv - Neuroscience 2023Quote: The 100-µm thickness coronal sections of the fresh mouse brain were prepared by vibrating microtome (Leica VT1200) in in saturated O2 /Glucose+ artificial cerebrospinal fluid (aCSF ...
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bioRxiv - Neuroscience 2024Quote: ... Coronal sectioning of mouse brains embedded in OCT was performed using a Leica Cryostat 1900 (Leica Microsystems, USA). From each mouse ...
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bioRxiv - Bioengineering 2024Quote: ... The foundation of the instrument was a rat stereotaxic (Mouse Specific Stereotaxic Base; Leica Microsystems, Inc., Richmond, IL) that allowed complete immobilization of the rat’s head and accurate positioning of the actuator relative to the superior-inferior axis of rotation of the left eye ...
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bioRxiv - Developmental Biology 2022Quote: Images from mouse and human samples were acquired with a Leica TCS SP8 confocal laser scanning microscope (Leica Microsystems). All images are shown as maximum intensity projections of acquired z-stacks of about one cell thickness (6-10 µm) ...
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bioRxiv - Developmental Biology 2022Quote: ... 10 μm transverse zebrafish and mouse cryosections were collected on a Leica CM1900 crysostat (Leica Biosystems, Buffalo Grove, IL). 10 μm paraffin mouse sections were collected on a Thermo Scientific Shandon Finesse ME Microtome (Thermo Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... transverse slices of 150µm from the hippocampi of a P17 mouse were prepared using a vibrating blade microtome (Leica) in cold (4°C ...
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bioRxiv - Developmental Biology 2020Quote: En face STED images of mouse cephalic explants were taken with a Leica SP8 TCS STED microscope (Leica Microsystems) equipped with a pulsed white-light excitation laser (WLL ...
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bioRxiv - Cancer Biology 2021Quote: ... A polymer detection system that includes an anti-mouse secondary antibody (DS9800, Novocastra Bond Polymer Refine Detection, Leica Biosystems) was then applied in the tumor samples ...
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bioRxiv - Neuroscience 2023Quote: ... The mouse brains were then cut into 20 µm thick frozen tissue sections using a cryostat (Leica, Cat# CM1950) and placed on Superfrost Plus Adhesion Microscopic glass slides (Epredia ...
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bioRxiv - Microbiology 2023Quote: ... FFPE mouse skin was processed and cut onto a polyethylene naphthalate (PEN) slide designed for LCM processing (Leica 11505158). At least 1,000 SGs or 1,000,000 μm2 of tissue was isolated to obtain enough material for RNA extraction ...
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bioRxiv - Immunology 2024Quote: ... Goblet cell number per crypt was counted for 20 crypts/mouse across the entire cecum using Aperio ImageScope (Leica) and ImageJ (NIH).
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bioRxiv - Immunology 2024Quote: ... and stained with a tertiary anti-mouse-HRP polymer reagent as per manufacturer recommendation (Leica Biosystems, Canada, Cat#DS9800).
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bioRxiv - Cancer Biology 2022Quote: ... Slides were washed with TBST and incubated with PowerVision Poly-HRP anti-rabbit IgG or anti-mouse IgG (Leica Biosystems) in a humidified chamber at 37°C for 30 minutes ...
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bioRxiv - Cell Biology 2020Quote: ... Ten pg of the mRNAs was injected into fully grown mouse oocytes using an IM-9B microinjector (Narishige) under a Dmi8 microscope (Leica) in M2 medium containing 10 µM milrinone ...
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bioRxiv - Cancer Biology 2021Quote: ... for 8 minutes at room temperature (Poly-HRP-mouse anti-rabbit IgG, BOND Polymer Refine Detection System, Leica, Germany; DS9800). A color reaction with 3,3-di-amino-benzidine (DAB chromogen ...
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bioRxiv - Cancer Biology 2021Quote: ... The slides were incubated with a secondary antibody (rabbit anti-mouse IgG, BOND Polymer Refine Detection System, Leica, Germany, DS9800) for 8 minutes at room temperature ...
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bioRxiv - Cancer Biology 2021Quote: ... Sections from a series of 17 tumour samples from the validation cohort were stained for CD8 (mouse monoclonal 4B11, Leica Biosystems PA0183 ...
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bioRxiv - Neuroscience 2020Quote: ... mouse or macaque slices (350 μm) were generated (Compresstome VF-300 vibrating microtome, Precisionary Instruments or VT1200S Vibratome, Leica Biosystems), with a block-face image acquired (Mako G125B PoE camera with custom integrated software ...
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bioRxiv - Genetics 2021Quote: Assessment of Aβ deposition was carried out by immunohistochemical staining of paraffin-embedded sections (3–5 μm-thick) with a mouse monoclonal (S6 F/3D) anti-Aβ antibody (Leica Biosystems Newcastle Ltd ...
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bioRxiv - Neuroscience 2022Quote: ... electroporated mouse pups were non-invasively screened at the age of P0 for cortical fluorescence using a fluorescence stereoscope (Leica MZ10f with X-Cite FIRE LED light source ...
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bioRxiv - Neuroscience 2022Quote: ... The mouse brains were frozen and sectioned sagittally with a thickness of 30 µm on a cryostat (Leica Biosystems, Germany), then the tissue slides were air dried for next step ...
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bioRxiv - Cancer Biology 2024Quote: ... Cryo-embedded mouse tumors (OCT compound, Tissue-Tek, #14291) were cut to 4-5µm with a cryostat (Leica CM 1950, Leica Biosystems) and positioned onto SuperFrost plus slides (R.Langenbrinck ...
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bioRxiv - Neuroscience 2023Quote: ... Coronal sections of the perfused mouse brains were cryosectioned into 40μm at −20C using a Leica CM3050 cryostat (Leica Biosystems). In order to detect GFP expression via immunohistochemistry staining ...
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bioRxiv - Neuroscience 2023Quote: Ambra1 WT and Het female mouse brain were cut into coronal sections (30 μm) with Leica CM1950 instrument (Leica Biosystems). Sections were blocked with 10% normal horse serum (NHS ...
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bioRxiv - Bioengineering 2023Quote: A representative brain from a mouse stimulated with DCZ (mouse 4N with the cranial window) was sectioned into 100 µm slices using a vibrating microtome (Leica). The slices were mounted and stained with DAAPI nuclear stain using ProLong Diamond antifade mountant with DAPI (Thermo Fisher P36962 ...
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bioRxiv - Biochemistry 2024Quote: ... The head of the mouse was positioned under the tip (5mm diameter) of the impactor (Leica Impact One Electromagnetic Impactor). The mouse was held over a sheet of filter floss to allow free linear and rotational movement of the head and neck ...
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bioRxiv - Developmental Biology 2024Quote: ... and whole-mounted mouse kidney explants were performed using (1) Leica SP8-X confocal fluorescence imaging system (Leica Microsystems, Germany), in 1024 x 1024 pixels using 25X water ...
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bioRxiv - Neuroscience 2024Quote: ... Hippocampal-containing sections were cut into 10 µm thick mouse brain coronal slices using a CM3050 S cryostat (Leica, Germany) at −20°C ...
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bioRxiv - Neuroscience 2024Quote: ... The frame was linked to a digital mouse brain atlas to guide the targeting of different brain structures (Angle Two Stereotaxic System, Leica Biosystems Division of Leica). The following stereotaxic coordinates in anteroposterior axis (AP) ...
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bioRxiv - Neuroscience 2024Quote: Coronal sections of the mouse brains were cut at a thickness of 6 μm via a microtome (Leica RM2245, Germany). Dewaxed and rehydrated hippocampal sections were blocked in TBST buffer (tris-buffered saline (TBS ...
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bioRxiv - Neuroscience 2021Quote: Acute mouse coronal slices containing hippocampus region (250 μm) were prepared from anesthetic mice using a vibrating tissue slicer (Leica VT1000S) in ice-cold artificial cerebrospinal fluid (ACSF ...
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bioRxiv - Genomics 2021Quote: ... primary antibody was diluted 1:10 in the peptide solution directly.) The primary antibodies were detected by 30 minute incubation with HRP-labeled anti-mouse secondary antibody (catalog# PV6114, Leica Microsystems) followed by detection with 3,3′-Diaminobenzidine (catalog# D4293 ...
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bioRxiv - Evolutionary Biology 2021Quote: Embryonic bat heads and embryonic E16.5 mouse heads were submerged in chilled lx PBS and imaged using a stereo microscope with brightfield illumination (Leica M165 FC) in standard anatomical views ...
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bioRxiv - Neuroscience 2021Quote: ... and −1.46 mm (dorsal hippocampus) from bregma according to the Paxinos and Franklin mouse brain atlas54 in triplicate using a cryostat (Leica, Germany) and dry mounted on coated Superfrost Plus slides (Thermo Fischer Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... mouse brain hemispheres prefixed with 4% PFA for 48 hours were sectioned at 50 μm using a Vibratome (Leica Microsystems Inc). Immunostaining of free-floating sections was performed using the ABC kit (Vector Laboratories ...
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bioRxiv - Neuroscience 2021Quote: Embryonic left and right hippocampus was microdissected from genotyped P21 mouse brains using a laser-assisted capture microscope (Leica ASLMD instrument) with Leica polyethylene naphthalate membrane slides as described in Lepagnol-Bestel et al ...