Labshake search
Citations for Leica :
201 - 250 of 250 citations for Nipah Virus Glycoprotein F Recombinant Protein Human Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2021Quote: Cryo-samples prepared from single-tagged CLTA-GFP expressing SK-MEL-2 cells and incubated with Alexa Fluor® 647 ChromPure human transferrin (Jackson Immuno Research Inc.) were imaged on a Leica EM Cryo CLEM system (Leica). The system consists of a Leica DM6 FS widefield microscope that is equipped with a motorized Leica EM Cryo stage and a short working distance (<0.28 mm ...
-
bioRxiv - Cancer Biology 2020Quote: ... In regions with CCF two to four consecutive cryosections with a thickness of 12 µm were put on membrane-slides (Leica Frame Slides, Nuclease and human nucleic acid free, PET-Membrane, 1,4 µm, Leica, Wetzlar, Germany) and incubated for one minute in 70% ethanol at −16°C ...
-
bioRxiv - Cancer Biology 2023Quote: ... Primary antibody used for Dp427 was the anti-human Dystrophin form Leica Biosystems (1:25, Dy4/6D3, NCL-DYS1, RRID:AB_442080, Leica Biosystems, Wetzlar, Germany) and the Dystrophin antibody from Abcam (1:100 ...
-
bioRxiv - Physiology 2024Quote: Human cells (A549 and BEAS-2B) were investigated using the TCS Sp5 Inverted Confocal laser scanning microscope (Leica Microsystems, Wetzlar, Germany) and a 20x /0.70 HC Plan Apochromat ...
-
bioRxiv - Cancer Biology 2021Quote: ... paraffin-embedded tissues was carried out on a Discovery XT automated slide stainer using a mouse anti-human monoclonal antibody for FOLR1 (Novocastra Clone BN3.2; Leica Biosystems, Wetzlar, Germany) at 15 µg/ml after antigen retrieval with Cell Conditioning 1 (CC1 ...
-
bioRxiv - Cell Biology 2020Quote: ... To compare the localization of the two proteins we used the SP8 STED microscope (Leica, with a 100× oil immersion objective and the LAS X Leica software) ...
-
bioRxiv - Biochemistry 2022Quote: ... Protein localization was visualized by a confocal laser scanning microscope (Leica TCS SP8; Leica Microsystems) with a 40X/1.30 oil immersion objective ...
-
bioRxiv - Biochemistry 2022Quote: ... Protein localization was visualized by a confocal laser scanning microscope (Leica TCS SP8; Leica Microsystems) with a 40X/1.30 oil immersion objective ...
-
bioRxiv - Cancer Biology 2021Quote: Formalin-fixed paraffin-embedded (FFPE) human skin tissue sections from organotypic tissue was stained for MITF (NCL-L-MITF, Leica Biosystems, Nussloch, Germany), MelanA (NCL-L-MITF ...
-
bioRxiv - Neuroscience 2020Quote: ... anti-glial fibrillary acidic protein (GFAP; mouse anti-human monoclonal antibody GA5, with bond heat-induced epitope retrieval, epitope retrieval time 10 min, PA0026; Leica Biosystems, Wetzlar, Germany); anti-ionized calcium-binding adapter molecule 1 (IBA1 ...
-
bioRxiv - Plant Biology 2020Quote: Cells expressing GFP-tagged proteins were examined using a Leica confocal microscope SP8X (Leica Microsystem, Germany) with 40×/1.0 water-dipping objective ...
-
bioRxiv - Cell Biology 2022Quote: ... Protein location of GATA1 and p50 were detected by immune-florescence using Confocal Microscopy (Leica DMI6000). Nuclear protein was isolated from baseline Zfp467+/+ and -/- BMSCs lysate by Pierce™ NE-PER® Nuclear and Cytoplasmic Extraction Reagent Kit (Life Technologies ...
-
bioRxiv - Cancer Biology 2022Quote: ... paraffin-embedded whole-tissue sections from decalcified human bone marrow core biopsy specimens in a Bond RX automated tissue stainer (Leica Biosystems, Buffalo Grove, IL), as described previously91 ...
-
bioRxiv - Systems Biology 2023Quote: ... paraffin-embedded whole-tissue sections from decalcified human BM core biopsy specimens in a Bond RX automated tissue stainer (Leica Biosystems, Buffalo Grove, IL), as described previously 80,81,87,88 ...
-
bioRxiv - Neuroscience 2023Quote: ... Cryosections from human and mouse spinal cord or DRGs were cut at 12 μm using a freezing microtome (CM1850; Leica, Buffalo Grove, IL, USA) and incubated at 4°C overnight with primary antibodies including ...
-
bioRxiv - Genetics 2021Quote: ... Protein localization was visualized by fluorescence confocal microscopy on a Leica SP5 microscope (Leica Microsystems, Wetzlar, Germany) and images were captured with LASAF as described previously [20].
-
bioRxiv - Cancer Biology 2020Quote: ... Protein expression was quantified as positive pixels using Aperio Positive Pixel Count Algorithm software (Leica Biosystem, IL).
-
bioRxiv - Cancer Biology 2020Quote: ... The expression of proteins was detected using the Bond Polymer Refine Detection kit (Leica Biosystems, Cat# DS9800) with diaminobenzidine as chromogen (88) ...
-
bioRxiv - Neuroscience 2022Quote: ... The images of the targeting proteins were analyzed by high solution confocal microscopy (LEICA TCS SP8, Germany). Control results using only the 2nd antibodies were shown in supplementary Fig ...
-
bioRxiv - Molecular Biology 2024Quote: ... Protein localization was analyzed 24-48 hours after infiltration by confocal laser scanning microscope (TCS SP8, Leica). GFP was excited at 488 nm and the fluorescence detected between 498-525 nm.
-
bioRxiv - Cell Biology 2020Quote: Prior to staining for collagen type II the sections were blocked with Novolink protein (Leica Biosystems, Weltzlar, Germany) for 5 minutes at room temperature ...
-
An annotation dataset facilitates automatic annotation of whole-brain activity imaging of C. elegansbioRxiv - Neuroscience 2019Quote: ... The fluorescence of the fluorescence proteins and the dye was observed sequentially using laser scanning confocal microscopy (Leica SP5 with 63× water immersion lens and 2× zoom) ...
-
bioRxiv - Biochemistry 2021Quote: ... proteins fixed in 30% ethanol/15% acetic acid and the gel piece embedded in tissue embedding media (Leica). After careful mounting on a cryo-holder ...
-
bioRxiv - Cell Biology 2024Quote: ... Cells that expressed the fluorescent fusion protein were identified using an epifluorescence microscopy (DM6000B, Leica Microsystems, Wetzlar, Germany) equipped with an oil immersion objective (1.4 NA ...
-
bioRxiv - Plant Biology 2021Quote: ... plant tissues expressing GFP-or RFP-fused proteins were imaged with a Leica TCS SP8 confocal microscope (Leica Microsystems) using the preset settings for GFP (Ex ...
-
bioRxiv - Cell Biology 2023Quote: ... Coverslips were incubated overnight at 4°C in primary antibodies targeted against p53 protein (CM5) (Leica Biosystems; 1:300) and GFP (Abcam #ab13970 ...
-
bioRxiv - Neuroscience 2024Quote: ... Slices were mounted once every 80 μm and mCherry fluorescent protein expression was detected under a microscope (Leica DMI6000). Microphotographs were taken at 2.5X magnification of each section where mCherry fluorescent signal was seen.
-
bioRxiv - Cell Biology 2021Quote: Images of immunostained cells or cells expressing fluorescent tagged proteins were acquired on Confocal inverted microscope SP8-X (DMI 6000 Leica). Optical sections were acquired with a Plan Apo 63x oil immersion objective (N.A ...
-
bioRxiv - Biochemistry 2020Quote: ... Protein mixture (5 μL) were deposited onto the grids and blotted for 3 seconds using an Automatic Plunge Freezer (Leica).
-
bioRxiv - Bioengineering 2020Quote: ... coli constitutively expressing the Green Fluorescent protein present on biofilms was examined by laser scanning confocal microscopy (Leica TCS SP8) using oil immersion at 40X magnification ...
-
bioRxiv - Plant Biology 2023Quote: ... Each 3 µl of protein reaction mix was loaded into a flow-chamber and immediately observed with a confocal microscope SP5 (Leica) equipped with a 60x oil objective (NA 1.42) ...
-
bioRxiv - Plant Biology 2024Quote: ... the eYFP fluorescence of fusion proteins was assayed 2–3 days after infiltration using a confocal microscope (Leica TCS SP8). For the Split–LUC assay ...
-
bioRxiv - Plant Biology 2024Quote: ... Strains exhibiting mNeonGreen fluorescence were used to localize the RMT2-mNeonGreen fusion protein by TCS SP8 confocal laser-scanning microscopy (Leica); excitation/emission settings were 488 nm/515-530 nm using the HyD SMD hybrid detector for mNeonGreen ...
-
bioRxiv - Plant Biology 2021Quote: ... the expression of TiAP1 and TaSYP51 proteins were observed and analysed using a confocal laser-scanning microscope (Leica SP5 - X, Germany) according to the methodology of Smigielski et al ...
-
bioRxiv - Cancer Biology 2020Quote: ... and after two washing steps with PBS the nonspecific binding of immunoglobulins was prevented by incubating the tissue slices with the Protein Block Novocastra Solution (Leica Biosystems) for 60 min ...
-
bioRxiv - Molecular Biology 2020Quote: Cells expressing EGFP protein were visualized 48 h post-transfection by fluorescent microscopy using Leica DMi8 inverted microscope (Leica Microsystems, Germany) at magnification 10x ...
-
bioRxiv - Cell Biology 2022Quote: ... Each protein mixture (14 μM for each component) was injected into a homemade chamber and imaged using a Leica microscope (Leica DMi8) using a 40X objective lens ...
-
bioRxiv - Cell Biology 2020Quote: ... To compare the localization of the two proteins we used the SP8 STED microscope (Leica, with a 100× oil immersion objective and the LAS X Leica software). Images were acquired as z-stacks with a z-step size of 120 nm and a X-Y resolution of 37.9 nm ...
-
bioRxiv - Plant Biology 2022Quote: ... the protein fluorescence was confirmed with the Leica TCS LSI macroscope with Plan APO 5x and 20x objectives (Leica Microsystems, Germany), using the settings described previously83 ...
-
bioRxiv - Cell Biology 2023Quote: ... as well as live tissues expressing fluorescently tagged proteins were imaged confocally using a Falcon SP8 laser scanning system (Leica Microsystems) with a 1.2-numerical aperture ...
-
bioRxiv - Biochemistry 2023Quote: ... the grids were blotted for 2 s to remove excess protein and rapidly plunged into liquid ethane (−182 °C) using a Leica EM GP2 (Leica Microsystems) operated at 95% humidity and 22 °C ...
-
bioRxiv - Biochemistry 2023Quote: ... the grids were blotted for 2 s to remove excess protein and rapidly plunged into liquid ethane (−182 °C) using a Leica EM GP2 (Leica Microsystems) operated at 95% humidity and 22 °C ...
-
bioRxiv - Plant Biology 2024Quote: ... The presence of Cas9 in the shoot apical meristem was investigated by observing the fluorescence of the Cas9-GFP protein (Venus-Cas9) using a confocal laser scanning microscope (TCS SP5, Leica Microsystems). The area of each cell within the SAM was calculated using SEM images of the SAM with Image J software (https://imagej.nih.gov/ij/download.html).
-
bioRxiv - Neuroscience 2022Quote: ... Figures from double immunofluorescence labeling of GLP-1R and GFAP proteins were obtained on a Confocal Leica SP5 microscope (Leica Microsystems, France).
-
bioRxiv - Cell Biology 2020Quote: Initial microscopic screening and quantitative examination of protein sorting in cultured mammalian cell lines was performed on a confocal Leica TCS SP5 II microscope (Leica GmbH, Heidelberg, Germany). Unless stated otherwise ...
-
bioRxiv - Plant Biology 2019Quote: For BiFC assays Yellow Fluorescent Proteins (YFP) were visualized using a Leica TCS-SP2 laser scanning confocal microscope (LSM) (Leica Microsystems, Heidelberg, Germany) with a 63×1.4 NA oil-objective lens and processed using Leica confocal software v.2.61 about 16 h post-transformation ...
-
bioRxiv - Cell Biology 2021Quote: ... the epithelium at ALI was visualized for Green Fluorescent Protein (GFP) at 20X by fluorescent microscopy using 3i Marianas/Yokogawa Spinning Disk Confocal Microscope (Leica Microsystems, TX, USA), assessed for functional phenotypes (as described below) ...
-
bioRxiv - Microbiology 2023Quote: ... Concentrations of stock solutions of NP and MP beads were calculated using a hemocytometer and a Leica DM IL microscope with a green fluorescent protein (GFP) filter (Leica microsystems, Wetzlar, Germany) with an attached CoolLED pE-300 ultraviolet light system (Andover ...
-
bioRxiv - Cell Biology 2022Quote: HEK293 cells expressing the fusion proteins eGFP-FAM76B and mCherry-tagged hnRNPA2B1 were visualized using a Leica TCS-SP8 confocal microscope (Leica Microsystems Inc., Shanghai, China).
-
bioRxiv - Plant Biology 2024Quote: ... benthamiana leaves were agroinfiltrated to transiently express the protein of interest and examined with a Leica TCS SP5 Laser Scanning Confocal Microscope (Leica Microsystems, Exton, PA, USA) using a 63x water immersion objective NA 1.2 (HCX PL APO) ...