Labshake search
Citations for Leica :
201 - 250 of 631 citations for Mac 1 SAP mouse human since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... and −1.46 mm (dorsal hippocampus) from bregma according to the Paxinos and Franklin mouse brain atlas54 in triplicate using a cryostat (Leica, Germany) and dry mounted on coated Superfrost Plus slides (Thermo Fischer Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... mouse brain hemispheres prefixed with 4% PFA for 48 hours were sectioned at 50 μm using a Vibratome (Leica Microsystems Inc). Immunostaining of free-floating sections was performed using the ABC kit (Vector Laboratories ...
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bioRxiv - Neuroscience 2021Quote: Embryonic left and right hippocampus was microdissected from genotyped P21 mouse brains using a laser-assisted capture microscope (Leica ASLMD instrument) with Leica polyethylene naphthalate membrane slides as described in Lepagnol-Bestel et al ...
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bioRxiv - Neuroscience 2022Quote: ... The mouse brains were dehydrated in 30% sucrose and sectioned at a thickness of 40 μm with a cryostat (Leica Microsystem), and the brain sections were immunostained for mCherry (1:1000).
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bioRxiv - Neuroscience 2021Quote: Mouse brains were fixed in 4% paraformaldehyde and cut into 40-μm-thick sections using a vibratome (Leica Biosystems, Nussloch, Germany). The sections were blocked using blocking solution (X0909 ...
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bioRxiv - Immunology 2020Quote: ... Non-invasive intravital imaging of mouse ear was performed using Leica DIVE (Deep In Vivo Explorer) inverted confocal microscope (Leica Microsystems) with full range of visible lasers (Spectra Physics) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... using microhematocrit capillary tubes (10 μL) and measured the hematocrit rate for each mouse using a Critocaps Micro-Hematocrit Capillary Tube Reader (Leica Microsystems) (22).
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bioRxiv - Neuroscience 2022Quote: ... then the right OB from each mouse was embedded in 6 % agarose and sliced coronally at 50 μm using a vibratome (Leica VT1000S).
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bioRxiv - Biophysics 2023Quote: 5 µm sections of formalin-fixed and paraffin-embedded (FFPE) mouse tail specimens were H&E-stained by using an automated stainer (Leica XL) at University of Manchester’s Histology Core as previously described [11 ...
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bioRxiv - Molecular Biology 2019Quote: ... Images were acquired using Leica DFC 3000G (Bin 1×1, Gamma1) camera and processed by Leica LAS X imaging software.
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bioRxiv - Neuroscience 2022Quote: In vivo images of mouse retinal structure were obtained with spectral domain-optical coherence tomography (SD-OCT) (Leica Microsystems, Buffalo Grove, IL). The mice received 1% tropicamide drops to stimulate eye dilation and were anesthetized with ketamine and xylazine (50 and 5 mg/kg body weight ...
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bioRxiv - Neuroscience 2022Quote: ... each mouse brain was sectioned into 30-μm-thick coronal slices with a freezing microtome (CM30503, Leica Biosystems, Buffalo Grove, IL, USA). The sections were first washed in PBS three times for 5 min each and then incubated in blocking solution containing 10% normal donkey serum (017-000-121 ...
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bioRxiv - Neuroscience 2021Quote: ... and coronal sections of the mouse brain containing the dentate gyrus and prefrontal cortex were then cut using a VT1200S microslicer (300 μm) (Leica, Wetzlar, Germany). Micro-dissected areas were post-fixed at 4C for 2 hours in 2% osmium tetroxide in 0.1mol/l Cacodylate Buffer ...
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bioRxiv - Neuroscience 2022Quote: ... each mouse brain was sectioned into 30-μm-thick coronal slices with a freezing microtome (CM30503, Leica Biosystems, Buffalo Grove, IL, USA). The sections were first washed in PBS three times for 5 min each and incubated in blocking solution for 2.5h at room temperature ...
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bioRxiv - Neuroscience 2019Quote: ... The left and right hippocampus was micro dissected from genotyped P21 mouse brains using a laser-assisted capture microscope (Leica ASLMD instrument) with Leica polyethylene naphthalate membrane slides as described in ...
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bioRxiv - Neuroscience 2021Quote: ... in 0.8% oxygen in an induction chamber (VetEquip, Livermore, CA) then placed in an Angle Two mouse stereotaxic frame (Leica Biosystems, Wetzlar, Germany) and secured with ear bars into a nose cone delivering isoflurane to maintain anesthesia ...
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bioRxiv - Biochemistry 2021Quote: ... Mouse brain tissue sections were sectioned at −21°C to a 12 μm thickness using a CM1850 Cryostat (Leica Microsystems, Wetzlar, Germany). Tissue sections were thaw mounted onto glass microscope slides (IMEB ...
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bioRxiv - Immunology 2020Quote: ... At least 150 cells per mouse were examined and quantified for cell type following the manufacturer’s instructions using a CME microscope (Leica, Buffalo Grove, IL) with a 40x objective ...
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bioRxiv - Neuroscience 2023Quote: ... A single 250 μm-thick parasagittal slice was cut for each mouse in this ice-cold solution using a sapphire blade (Delaware Diamond Knives, Wilmington, DE) on a vibratome (VT1000S; Leica, Deerfield, IL). Slices were then recovered at 32°C for 20-30 minutes in oxygenated artificial cerebrospinal fluid (ACSF ...
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bioRxiv - Neuroscience 2023Quote: ... in 0.8% oxygen in an induction chamber (VetEquip, Livermore, CA) and placed in an Angle Two mouse stereotaxic frame (Leica Biosystems, Wetzlar, Germany). Mice were given a subcutaneous injection of meloxicam (2 mg/kg ...
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bioRxiv - Cancer Biology 2021Quote: ... and p53 (D07, 1:50, Leica) were applied with negative controls as previously described2.
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bioRxiv - Cancer Biology 2022Quote: ... ER Solution 1 (AR9961, Leica Biosystems) was used ...
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bioRxiv - Cell Biology 2019Quote: The gastrocnemius and quadriceps muscles were isolated from mouse hind limbs and dehydrated in a 30% sucrose solution and then sectioned in a constant cold section box (Leica CM 3000, Germany) at −20 °C ...
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bioRxiv - Systems Biology 2023Quote: ... the mouse brains were sectioned coronally into 40 μm-thick slices at −18 to −20 °C using a microtome (Leica Microsystems, Wetzlar, Germany). Floating brain sections (fixed in 4% PFA ...
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bioRxiv - Cell Biology 2023Quote: Fresh mouse kidneys were embedded in low-melting agarose and cut into 300 μm slices using a vibratome (LEICA VT1200 S, Mannheim, Germany). Slices were maintained in carbogenated Krebs-Henseleit-Buffer.14 AKS were incubated with 5 μM propidium iodide for 5 min to label cells damaged by cutting.
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bioRxiv - Cancer Biology 2024Quote: Multiplexed immunofluorescence imaging: Cryo-embedded mouse tumors (OCT compound, Tissue-Tek, #14291) were cut to 4-5µm with a cryostat (Leica CM 1950, Leica Biosystems) and positioned onto SuperFrost plus slides (R.Langenbrinck ...
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bioRxiv - Developmental Biology 2019Quote: ... β-Dystroglycan (1:100; Leica #NCL-b-DG), aPKCς clone C-20 (1:400 ...
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bioRxiv - Cancer Biology 2021Quote: ... CD3 (clone LN10, dilution 1:500, Leica), CD14 (clone SP192 ...
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bioRxiv - Cell Biology 2021Quote: ... for 1 hour and visualised by Leica Sp8 inverted confocal microscopy in serial sections of 20 μm ...
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bioRxiv - Molecular Biology 2022Quote: ... eMyHC (Leica NCL-MHC-d;1:200), laminin (Sigma-Aldrich L9393 ...
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bioRxiv - Developmental Biology 2023Quote: ... rabbit anti-p53 (1:1000; Leica Biosystems), rabbit-anti-active caspase-3 (1:1000 ...
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bioRxiv - Cell Biology 2024Quote: ... Epitope Retrieval Solution 1 (Leica Cat#AR9961), Epitope Retrieval Solution 2 (Leica Cat#AR9640) ...
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bioRxiv - Cell Biology 2024Quote: ... eMyHC (Leica NCL-MHC-d; 1:200) for staining of muscle cryosections ...
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bioRxiv - Molecular Biology 2021Quote: Facet joint cartilage and synovium (containing a thin layer of fibrous capsular tissue due to technical difficulties in obtaining pure synovium from mouse facet joints) were freshly dissected under a microscope (LEICA M80, with a LEICA IC80 HD video camera). Cartilage and synovium samples were collected separately in RNAlater solution (Ambion ...
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bioRxiv - Physiology 2019Quote: ... Fast red detection of mouse Gdf15 was performed on the Bond RX using the Bond Polymer Refine Red Detection Kit (Leica Biosystems, Cat No. DS9390) according to the ACD protocol ...
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bioRxiv - Physiology 2021Quote: ... Fast red detection of mouse Rxfp4 was performed on the Bond Rx using the Bond Polymer Refine Red Detection Kit (Leica Biosystems, Cat No. DS9390) with an incubation time of 20 min ...
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bioRxiv - Developmental Biology 2021Quote: ... Then the samples were embedded in a mixture of OCT and water at a ratio of 1:1 and sectioned at 10 μm thickness with freezing microtome (Leica). The mRNA detection by in situ hybridization was performed as previously described (Lauter et al. ...
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bioRxiv - Neuroscience 2021Quote: ... the tissue was trimmed to less than 1 × 1 mm and one side was exposed using an ultramicrotome (UC7, Leica), then turned downwards to be remounted to a metal pin with conductive silver epoxy (CircuitWorks ...
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bioRxiv - Developmental Biology 2021Quote: ... The tissues were merged in 10% and 30% sucrose-PBS solutions at RT (2 h each solution) followed by 1:1 solution 30% sucrose:tissue-freezing medium (Leica 14020108926). Afterwards ...
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bioRxiv - Neuroscience 2021Quote: ... microglial density quantifications and Iba-1:P2RY12 or Iba-1:TMEM119 colocalization analysis 40 µm free-floating sections were cut with a sliding microtome (Leica) and stained with relevant antibodies in 10% normal goat serum (NGS ...
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bioRxiv - Biochemistry 2019Quote: ... The cells were mounted in 1:1 glycerol-PBS and imaged using a fluorescence microscope (Leica DMi8, Leica, Wetzlar, Germany). Fluorescent focus units (FFU ...
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bioRxiv - Biochemistry 2019Quote: ... The cells were mounted in 1:1 glycerol-PBS and imaged using a fluorescence microscope (Leica DMi8, Leica, Wetzlar, Germany). Fluorescent focus units (FFU ...
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bioRxiv - Microbiology 2019Quote: ... the samples were mounted in PBS-glycerol (1:1) and visualized by confocal microscopy (Leica TCS-SP2, Leica Microsystems, Germany).
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bioRxiv - Microbiology 2019Quote: ... the samples were mounted in PBS-glycerol (1:1) and visualized by confocal microscopy (Leica TCS-SP2, Leica Microsystems, Germany).
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bioRxiv - Neuroscience 2022Quote: ... Sakura Finetek) and 30% sucrose–PBS (1:1) for cryosectioning (25 μm-thick sections) using a Leica Cryostat (Leica, CM1860). For immunofluorescence staining ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... The gonadal tissues for histology were dehydrated in a tissue processor (TP 1020-1-1, Leica Microsystems Nussloch GmbH, Germany) and thereafter embedded in paraffin ...
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bioRxiv - Genomics 2023Quote: ... slides were deparaffinized and automatically stained with specific antibodies against synovial cell markers (PECAM-1, DAKO JC/70a, 1:10; CD20, Ventana Roche L26, prediluted; CD3, Leica LN10 ...
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bioRxiv - Cancer Biology 2020Quote: ... was diluted 1:100 in Antibody Diluent (Leica).
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bioRxiv - Microbiology 2021Quote: ... SARS-CoV-2 Nucleoprotein: 1:2000 (Leica, AR9961) 30minutes ...
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bioRxiv - Immunology 2021Quote: ... anti-CD20 (clone L26, 1:200, Leica Biosystem), anti-CD31 (clone PECAM-1 ...