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Citations for Leica :
201 - 250 of 2163 citations for 6 Methyl 5 4 phenyl 1 3 thiazol 2 yl 2 trifluoromethyl nicotinic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: ... for all but CD206 which utilized AEC (Cat# K400511-2, Leica, Heidelberg, Germany). A hematoxylin (Cat #K800821-2 ...
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bioRxiv - Molecular Biology 2024Quote: ... and subjected to epitope retrieval with Bond ER solution 2 (Leica Biosystems, #AR9640). Antibody labeling was then accomplished by 4 sequential rounds of primary antibody incubation ...
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bioRxiv - Microbiology 2024Quote: ... Heat-induced antigen retrieval was performed using Epitope Retrieval 2 solution (Leica Biosystems) for 20 min ...
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bioRxiv - Microbiology 2024Quote: ... After 20 minutes of HIER with EDTA (Bond Epitope Retrieval Solution 2, Leica), tissue was blocked with Ultracruz (sc-516214 ...
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bioRxiv - Plant Biology 2023Quote: Nodules were embedded in 4-5% agarose and sliced in 100um sections using a vibratome (Leica). Samples were immediately imaged on slides using a 20x air objective using the excitation and emission settings described above ...
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bioRxiv - Neuroscience 2020Quote: ... Cryosections were prepared from tissue embedded in a 2:1 mixture of 30% sucrose: OCT (Sakura Finetek, catalog #4583) onto X-tra slides (Leica Microsystems, catalog #3800200). Tissue sections were dried ...
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bioRxiv - Molecular Biology 2022Quote: ... Green siliques were harvested 12 d after flowering from 4-6 week old plants and opened using fine needles under a dissecting microscope (Leica MZ9.5, Germany). Images of opened siliques were captured using a Leica DFC290 (Germany ...
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bioRxiv - Neuroscience 2023Quote: ... Images of cultured ependymocytes co-treated with Sir-Actin and calcein AM were captured at 10-s intervals for 12 minutes while that of co-treated with Sir-Actin and fluo-4 AM were captured at 10-s intervals for 6 minutes using confocal scanning microscope (DMi8, Leica, Wetzlar, Germany). The intensity of F-actin area from all Sir-Actin positive cells in a field of recording were included in the statistics ...
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bioRxiv - Neuroscience 2023Quote: ... The fluorescence signals of Fluo-4 were monitored at 10-s intervals for 6 minutes by confocal scanning microscope (DMi8, Leica, Wetzlar, Germany). As previously described 46 ...
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bioRxiv - Molecular Biology 2020Quote: ... 4 μl of this mixture was back side blotted for 6 s at relative humidity 98% and temperature 19°C on a glow-discharged holey carbon grid (CF-2/1-3C, Protochips) before plunge-freezing in liquid ethane (Leica EM GP2 automatic plunger). Dose-symmetrical tilt series acquisition 51 was performed on an FEI Titan Krios electron microscope operated at 300 kV using a Gatan Quantum energy filter with a slit width of 20 eV and a K2 or K3 direct detector operated in counting mode ...
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bioRxiv - Cancer Biology 2021Quote: ... Agilent M087601-2, used at a dilution of 1/50 for 15mins at room temperature. HIER was performed on-board using Leica ER1 solution (low pH) for 20 minutes ...
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bioRxiv - Immunology 2022Quote: ... Mouse tissues (at least 2 mm2/organ) were imaged by confocal microscopy (SP8, Leica) using 40x oil objective ...
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bioRxiv - Cell Biology 2021Quote: ... followed by cryo-immobilization in the EMPACT-2 HPF apparatus (Leica Microsystems; Vienna Austria) as described (Largeau & Legouis ...
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bioRxiv - Systems Biology 2021Quote: ... Mice were anesthetized with 2% isoflurane in oxygen using a precision vaporizer (Leica Biosystems). In each group ...
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bioRxiv - Plant Biology 2021Quote: ... and then transferred at −90 °C into an AFS 2 freeze-substitution device (Leica). Grafts were incubated in a cryosubstitution mix containing only uranyl acetate 0.1 % in pure acetone for 30 h (freeze substitution and embedding protocol adapted from Nicolas et al. ...
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bioRxiv - Genetics 2021Quote: ... Stained 2-day-old animals were analysed by a vertical fluorescent microscope (DM2500, Leica). We selected the area between two pharyngeal bulbs to evaluate the number of β-amyloid deposits ...
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bioRxiv - Developmental Biology 2022Quote: Widefield images were acquired every 2 min with an M205 fluorescent dissection microscope (Leica) equipped with a PLANAPO 2.0x objective ...
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bioRxiv - Cell Biology 2022Quote: ... and 2% FBS) using a Leica EM PACT2 high-pressure freezing unit (Leica Microsystems) with rapid transfer system at high pressure (2100 bar) ...
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bioRxiv - Developmental Biology 2022Quote: ... They were fixed by high pressure freezing with the EMPACT-2 system (Leica Microsystems). Cryosubstitution (FS ...
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bioRxiv - Cell Biology 2024Quote: ... followed by cryo-immobilization in the EMPACT-2 HPF apparatus (Leica Microsystems; Vienna Austria) as described (Largeau & Legouis ...
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Recruitment of MRE-11 to complex DNA damage is modulated by meiosis-specific chromosome organizationbioRxiv - Genetics 2020Quote: ... Figure 4 and Figure S3): 5-10 adult worms were placed on an uncharged slide (Surgipath Leica) in 5µl M9 solution ...
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bioRxiv - Biophysics 2024Quote: ... After blotting for 4-5 s in a Leica EM GP2 plunge freezer (Leica Microsystems, Wetzlar, Germany) with the chamber set to 5°C and 95% humidity ...
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bioRxiv - Immunology 2022Quote: ... 3′-3-diaminobenzidine (DAB) substrate (Leica Microsystems) was used as a chromogen followed by hematoxylin counterstain ...
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bioRxiv - Immunology 2024Quote: ... and single-cell quantification was performed in 3–5 fields imaged with the ACS_APO 20x/NA 0.60 objective of a confocal microscope (Leica, SPE). Mean Fluorescence Intensity (MFI ...
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bioRxiv - Pathology 2022Quote: ... Pictures of 4-6 different fields per sample were taken under a Leica DM5000 B microscope equipped with a digital CCD (Leica Camera, Wetzlar, Germany).
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bioRxiv - Neuroscience 2020Quote: ... Brains were embedded in agar (3-4%) and coronal sections (50 µm) were cut on a vibrating microtome (Leica VT1000S) and collected in a 1 in 4 series ...
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bioRxiv - Cell Biology 2021Quote: ... Dried samples were then coated with 3 nm platinum and 4 nm carbon using an ACE600 coating device (Leica Microsystems). The sample grids were imaged using a JEOL JEM2800 scanning/transmission electron microscope at 200 kV.
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bioRxiv - Plant Biology 2022Quote: ... The samples were then coated with 3 nm platinum and 4 nm carbon using the ACE600 coating device (Leica Microsystems).
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bioRxiv - Physiology 2024Quote: ... the tissues were cleared with methyl benzoate and embedded in paraffin (Paraplast plus, Leica Biosystems, Wetzlar, Germany). The paraffin sections were cut at a thickness of 10 μm using a Leica Biocut 2035 (Leica Biosystems ...
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bioRxiv - Physiology 2024Quote: ... apparatus The tissues were embedded in paraffin (Roth) sliced in 4-5 μm sections using a microtome (Leica). The sections underwent deparaffination and rehydration procedures ...
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bioRxiv - Bioengineering 2024Quote: ... the samples were paraffin-embedded and cut into 4–5 μm sections using a microtome (RM2125, Leica; Germany). These sections were then stained with hematoxylin and eosin (H&E ...
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bioRxiv - Neuroscience 2021Quote: ... contusion was induced using a 2 mm convex tip attached to an electromagnetic impactor (Leica). The contusion depth was set to 1.25 mm from dura with a velocity of 4.0 m/s sustained for 300 ms ...
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bioRxiv - Plant Biology 2021Quote: ... 9.3% water in water-free acetone in a Leica AFS 2 (Leica Microsystems, Wetzlar, Germany) as described previously (Flechsler et al. ...
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bioRxiv - Neuroscience 2020Quote: ... Slides underwent heat-induced epitope retrieval with Epitope Retrieval Solution 2 (pH 9.0, Leica AR9640) at 95°C for 5 mins ...
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bioRxiv - Cancer Biology 2022Quote: ... and CD8 alpha antigen retrieval was performed with BOND Epitope Retrieval 2 – ER2 (AR9640, Leica) for 40 ...
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bioRxiv - Neuroscience 2021Quote: ... 2 μm z-steps) were taken using a laser scanning confocal microscope (TCS SP8, Leica). Three sections of 20 μm depth each were averaged per animal for analysis ...
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bioRxiv - Developmental Biology 2022Quote: ... Slides underwent heat-induced epitope retrieval with Epitope Retrieval Solution 2 (pH 9.0, Leica AR9640) at 95°C for 5 mins ...
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CXCR4-binding PET tracers link monocyte recruitment and endothelial injury in murine atherosclerosisbioRxiv - Pathology 2020Quote: ... aortas were placed between 2 clear sheets and imaged by M205 FA stereoscope system (Leica).
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bioRxiv - Cell Biology 2020Quote: ... L4 animals were fixed by high-pressure freezing with the EMPACT-2 system (Leica Microsystems). Freeze-substitution (FS ...
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bioRxiv - Immunology 2021Quote: ... samples underwent heat induced antigen retrieval (HIER) in BOND Epitope Retrieval 2 (AR9640, Leica Biosystems) or BOND Epitope Retrieval 1 (AR9961 ...
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bioRxiv - Immunology 2024Quote: ... Heating mediated-epitope/antigen retrieval was performed with Bond TM Epitope Retrieval 2 (Leica Biosystems). Blocking was performed with plant-based protein blocking buffer (Cell Signaling Technology [CST] ...
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bioRxiv - Cancer Biology 2024Quote: ... followed by an initial antigen retrieval using Epitope retrieval solution 2 (Leica Bond ER2, #AR9640) at 95°C for 30 min ...
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bioRxiv - Microbiology 2023Quote: ... the samples were warmed up slowly (2 °C/h) to - 60 °C (AFS2; Leica Microsystems). After 8 to 12 h ...
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bioRxiv - Plant Biology 2023Quote: ... and images were taken every 20 min for 2 days using microscope (LEICA M205 FA). Imaging data were analyzed with ImagJ (FIJI).
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bioRxiv - Bioengineering 2022Quote: ... and the appropriate visualization system was applied – DAKO DAB (Cat# K346811-2, Leica, Heidelberg, Germany) for all but CD206 which utilized AEC (Cat# K400511-2 ...
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bioRxiv - Cell Biology 2023Quote: Freeze substitution was performed in a Leica EM AFS-2 freeze substitution unit (Leica Microsystems) as described previously (Bragulat-Teixidor et al. ...
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bioRxiv - Developmental Biology 2024Quote: ... the stained larvae were mounted in 2 % methylcellulose and photographed by a fluorescent stereomicroscope (Leica) or mounted in low-melting agarose gel and photographed by a confocal microscope (Olympus FV1000).
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bioRxiv - Biochemistry 2024Quote: ... Grids were blotted for 2 s with an EM GP2 Plunge Freezing device (Leica Microsystems) held at 4 °C and 90% humidity before freezing in liquid ethane ...
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bioRxiv - Neuroscience 2022Quote: ... 0.4 mM L-ascorbic acid and the pH was adjusted to 7.4) and then sliced on a vibratome (Leica, VT1200 S, Deerfield, IL, USA). Brain slices (300 μm thick ...
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bioRxiv - Biochemistry 2020Quote: ... Protein mixture (5 μL) were deposited onto the grids and blotted for 3 seconds using an Automatic Plunge Freezer (Leica).