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Citations for Leica :
201 - 250 of 1513 citations for 4 Bromo 3' 1 3 dioxolan 2 yl 2 fluorobenzophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... Tissue sections were incubated in 3% hydrogen peroxide (Bond Polymer Refine Detection Kit DS9800, Leica Biosystems) for 15 mins to block endogenous peroxidases ...
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bioRxiv - Evolutionary Biology 2021Quote: ... embedded in 3% LM-agarose (Roth) and cut 250 µm-thick with a vibratome (Leica VT1000S). Slices were then transferred on Millicell inserts (Merck Millipore ...
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bioRxiv - Cell Biology 2020Quote: Broad-band autofluorescence was acquired from sections cut at 3 μm using X20 objective (Leica DM550B). Sections were excited at 458 nm and fluorescence emission captured above 475 nm ...
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bioRxiv - Cancer Biology 2022Quote: ... and glypican 3 (Cell Marque, #261M) was detected using BOND Polymer Refine Detection (Leica Biosystems, #DS9800), including a hematoxylin counterstain to visualize nuclei.
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bioRxiv - Cell Biology 2022Quote: ... and 3% water in acetone) and placed in an automated freeze substitution machine (AFS2, Leica Microsystems). A freeze substitution protocol was used as previously described16 ...
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bioRxiv - Neuroscience 2023Quote: High-pressure freezing: Reprogrammed cortical neurons were seeded and grown on 3 mm sapphire discs (Leica) until PID30 ...
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bioRxiv - Cancer Biology 2023Quote: ... the slides were incubated with primary antibodies (Table 3) in Bond primary antibody diluent (AR9352, Leica) followed by secondary antibodies (Leica ...
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A synthetic elastic protein as molecular prosthetic candidate to strengthen vascular wall elasticitybioRxiv - Cell Biology 2023Quote: ... followed by thin (∼3 nm) copper sputtering (Bal-tec MED 020, Leica Microsystemes SAS, Nanterre, France). Imaging was carried out using a Hitachi S800 FEG ...
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bioRxiv - Developmental Biology 2023Quote: Fixed brains were embedded in 3% noble agar gel and sectioned with the Vibratome VT1000S (Leica) at 50 μm thickness as free-floating sections ...
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bioRxiv - Physiology 2021Quote: Immunohistochemical staining in pancreas and fat sections was carried out on 1-2 μm section cut from paraffin blocks in an automated immunostainer from Leica biosystems (BOND RX ...
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bioRxiv - Immunology 2021Quote: ... Sections were serially imaged over the course of 1-2 hours and compiled using LAS X software (Leica Microsystems, USA). Image analysis was performed using Imaris software (Bitplane ...
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bioRxiv - Cancer Biology 2022Quote: ... with antigen retrieval at 100ºC for 20 min with Epitope Retrieval Solution 1 or 2 respectively (AR9961, AR9640, Leica Biosystems). TMAs were blind-scored by multiplying staining intensity with staining coverage ...
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bioRxiv - Neuroscience 2023Quote: ... the animals were placed under 0.8-1% isoflurane anesthesia (2% used for induction) and imaged on an upright laser-scanning confocal (Leica SP8) or upright laser-scanning two-photon microscope (Bruker Ultima ...
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bioRxiv - Developmental Biology 2023Quote: ... dehydrated in methanol and cleared in Benzyl Alcohol/Benzyl Benzoate (1/2) prior to mounting and imaging with a SP8X confocal microscope (Leica). A 3D binary mask was built using Fiji to obtain a binary image of the tissue ...
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bioRxiv - Cell Biology 2024Quote: ... and EB3 comets in single cells were imaged for 60 seconds (1 frame per 2 seconds) with a Leica DMi8 microscope (Leica). Only cells exhibiting both tdTomato and EYFP signals were included in the analysis ...
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bioRxiv - Neuroscience 2021Quote: ... Slices were visualized on a resonant scanning 2-photon system (Leica) and Z-stack images were taken ...
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bioRxiv - Cancer Biology 2020Quote: ... followed by pretreatment with Epitope Retrieval Solution 2 (AR9649, Leica Biosystems) for 20 minutes and then blocking buffer for 10-20 minutes ...
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bioRxiv - Immunology 2023Quote: ... Fura-2-AM was excited using a monochromatic light source (Leica) coupled into an inverted Leica DMI4000B microscope that was equipped with a 20x objective (HCX PL Fluotar 20x ...
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bioRxiv - Cancer Biology 2023Quote: ... anti-BCL2 (Clone: BCL-2/100/D5; RTU; Leica Biosystems; #PA0117), anti-BCL6 (Clone:LN22 ...
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bioRxiv - Molecular Biology 2023Quote: ... sputter-coated with 2 nm carbon using ACE600 sputter coater (Leica).
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bioRxiv - Neuroscience 2024Quote: ... and 2 CaCl2) using a vibratome (VT 1200, LEICA, Wetzlar, Germany) and afterwards incubated in ACSF at 36 °C for 45 min ...
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bioRxiv - Cell Biology 2020Quote: ... Images were prepared with the Leica Application Suite – Advanced Fluorescence (LAS-AF) Version 3 software (Leica Microsystems).
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bioRxiv - Neuroscience 2020Quote: ... the Ca2+ signal within the SNs was captured during 3-min using Leica DM6000 microscope (Leica Microsystems) and processed with the software Fiji image J ...
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bioRxiv - Neuroscience 2020Quote: ... slices were mounted in 3% agarose and sliced using a vibratome (Leica VT1200S; Leica Microsystems; Bannockburn, IL) to a thickness of 30 µm ...
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bioRxiv - Cell Biology 2020Quote: ... The surface area of cardiomyocytes stained by MHC was determined with image analysis software (Leica Qwin 3) and calculated as the mean of 100 to 120 cells from randomly selected fields.
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bioRxiv - Cell Biology 2021Quote: ... were recorded and the maximal projection was deconvoluted with 3 iterations by the LAS AF 2.7.3.9 software (Leica).
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bioRxiv - Biochemistry 2021Quote: ... for 3 sec before plunge freezing in liquid ethane using a Leica EM Grid Plunger (Leica Microsystems). Datasets were collected at the NIH Multi-Institute Cryo-EM Facility (MICEF ...
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bioRxiv - Physiology 2020Quote: ... mexicanum gametes was imaged using FluoZin-3 and a TCS SP5 confocal microscope (Leica Microsystems, Wetzlar, Germany) equipped with a Leica 506224 5X objective ...
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bioRxiv - Neuroscience 2020Quote: ... Bregma −1.5 to −3) were cut in ice cold carbogenated cutting solution using a vibratome (VT1200S, Leica). Slices were incubated in cutting solution in a submerged chamber at 34°C for at least 45 min and then transferred to ACSF in a light-shielded submerged chamber at room temperature (21°C ...
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bioRxiv - Neuroscience 2021Quote: ... Bregma −1.5 to −3) were cut in ice cold carbogenated cutting solution using a vibratome (VT1200S, Leica). Slices were incubated in cutting solution in a submerged chamber at 34°C for at least 45 min and then transferred to ACSF in a light-shielded submerged chamber at room temperature (21°C ...
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bioRxiv - Neuroscience 2021Quote: ... Brains were sliced in 30 μm coronal sections into 3 series on a cryostat (Leica CM3050 S) and stored free-floating in antifreeze cryoprotectant [0.25 mM polyvinylpyrolidone ...
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bioRxiv - Cell Biology 2022Quote: Cells attached to 3-mm sapphire discs were cryo-immobilized using an EM ICE (Leica Microsystems, Austria) and 6-mm sapphire discs were frozen by using a Compact 03 high-pressure freezer (M ...
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bioRxiv - Neuroscience 2022Quote: ... They were embedded in 3% agar and 200 µm thick sections cut using vibratome (VT1000S, Leica, GmbH). Sections were mounted onto gelatin-coated slides ...
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bioRxiv - Developmental Biology 2024Quote: ... larvae were imaged in 3% methylcellulose on a binocular microscope using Leica Application Suite (LAS 3.3, Leica). After imaging ...
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bioRxiv - Neuroscience 2023Quote: ... Brains were embedded in 3% agarose and cut into 100μm thick sections using a VT1000S vibratome (Leica). Sections were incubated O/N in primary antibody solution containing 5% NGS and 0.3% triton ...
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bioRxiv - Cell Biology 2022Quote: ... and images were acquired using a 3-channel TCS SP2 laser-scanning confocal microscope (Leica Mycrosystems, Germany) and STELLARIS 8 ...
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bioRxiv - Molecular Biology 2023Quote: 2.8 × 105 A549 or A549-IFITM3 cells were seeded on carbon- coated 3 mm sapphire discs (Leica) in a 6-well plate in complete growth medium (DMEM-F12 ...
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bioRxiv - Immunology 2024Quote: ... Nine TLS+ Glioma FFPE sections (3 µm) were generated using the Leica SM 2000R microtome (Leica Microsystems), and incubated at 37 C overnight ...
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bioRxiv - Cell Biology 2024Quote: ... synovial tissue sections at 3 µm thickness were prepared using a HistoCore Autocut microtome (Leica Biosystems, Switzerland) from FFPE blocks and transferred onto Superfrost® Plus Menzel (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... and the contusion was induced using a 3 mm convex tip attached to an electromagnetic impactor (Leica). The contusion depth was set to 0.95 mm from dura with a velocity of 4.0 m/s sustained for 300 ms ...
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bioRxiv - Cell Biology 2022Quote: ... pre-blotted and blotted with filter paper (Whatman 1) for 2 sec followed by plunging into liquid ethane using a Leica EM GP (Leica Microsystems). The samples were stored in liquid nitrogen until data acquisition ...
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bioRxiv - Cell Biology 2021Quote: ... and freeze substituted in anhydrous acetone containing 1 % OsO4/ 2% H2O for 64h using the Automatic Freeze Substitution unit (AFS; Leica Microsystems). The samples were included in Epon ...
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bioRxiv - Neuroscience 2022Quote: ... mice were anesthetized with a mixture of oxygen (1 L/min) and isoflurane 2% (Baxter AG, Vienna, Austria) and placed in a stereotactic frame (Angle One; Leica, Germany). The skin was locally anesthetized with 40– 50 μL lidocaine 0.5% and disinfected with betadine.
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bioRxiv - Cell Biology 2022Quote: ... and freeze substituted in anhydrous acetone containing 1% OsO4/2% H2O for 64 h using the Automatic Freeze Substitution unit (AFS; Leica Microsystems). The samples were included in EMbed 812 ...
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bioRxiv - Biochemistry 2023Quote: ... Frozen samples collected on metal carriers were transferred under liquid nitrogen into a pre-frozen cryotube containing 1 ml freeze-substitution solution (2% OsO4 in 100% acetone with 1% lecithin) and finally to a freeze substitution unit (Leica EMAFS) for processing ...
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bioRxiv - Neuroscience 2020Quote: ... embedded with agarose 3% in PBS 0.01M and cut in slices of 80μm of thickness in a vibratome (Leica). Slices were incubated for 1h at room temperature in a blocking solution containing 1% BSA and 0.3% Triton X-100 in PBS 0.01M ...
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bioRxiv - Neuroscience 2020Quote: ... embedded in 3% agarose-PBS and cut 50–100 μm in thickness using a vibrating microtome (Leica, VT100S). Sections were placed in blocking solution containing 10% Normal Goat Serum (NGS ...
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bioRxiv - Neuroscience 2021Quote: ... embedded in 3% agarose-PBS and cut 50–100 μm in thickness using a vibrating microtome (Leica, VT100S). Sections were placed in blocking solution containing 10% Normal Goat Serum (NGS ...
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bioRxiv - Immunology 2021Quote: ... Tissues were routinely processed for histopathology on a Leica Peloris 3 Tissue Processor (Leica Biosystems, Buffalo Grove, IL), embedded in paraffin ...
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bioRxiv - Microbiology 2021Quote: ... Confocal imaging was performed with a Leica TCS SP8 STED 3× laser scanning confocal microscope (Leica, Wetzlar, Germany) equipped with a 100×/1.4 Oil HC PL APO CS2 STED White objective ...