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Citations for Leica :
201 - 250 of 1240 citations for 4 3 6 Dimethyl 3 heptyl phenol monoethoxylate ring 13C6 solution since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2022Quote: ... The embryos were cleared in xylene and processed for paraffin 7µm sections as described before [3] using microtome (RM2035, Leica, Germany). Following deparaffinization and rehydration ...
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bioRxiv - Cell Biology 2020Quote: ... elastic modulus and dissipation were performed on a Catalyst BioScope atomic force microscope (Bruker, Santa Barbara, CA) combined with a 3-channel confocal microscope TCS SP5 II (Leica; 20×/NA 0.70 or 40×/NA 0.85 air objectives) ...
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bioRxiv - Bioengineering 2020Quote: ... Briefly, mice were anesthetized with isoflurane (3% induction, 1.5% maintenance and secured on a stereotaxic frame (Leica Microsystems, Wetzlar, Germany). A 3 mm craniotomy (−1 AP mm bregma ...
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bioRxiv - Plant Biology 2021Quote: ... Cells cultured in M9 or LS medium containing 3% sucrose were added to flat specimen carriers and frozen using a high-pressure freezing machine (Leica EM PACT ...
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bioRxiv - Cell Biology 2021Quote: ... 7F and S5D are average projections of time-lapse movies acquired a maximum speed during 3 minutes using a spinning-disk microscope composed of an inverted microscope (DMI4000B; Leica) equipped with an HCX Plan Apochromat 100 × /1.46 NA oil objective (PerkinElmer ...
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bioRxiv - Cell Biology 2021Quote: ... Grids were blotted between 2.5 and 3 sec and plunged frozen in liquid ethane using a Leica vitrification plunger (EM GP2, Leica Microsystems). For RiboRad6mix ...
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bioRxiv - Neuroscience 2020Quote: ... Spinal cords were removed from the spinal column by hydraulic extrusion and embedded in low-melting point agarose (2-3%) before sectioning using a vibratome (Leica) into 300 µm thick coronal slices in ice-cold oxygenated ACSF ...
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bioRxiv - Biochemistry 2022Quote: ... The cells were fixed with 3% formaldehyde and permeabilized with 0.1% Triton X-100 and imaged in Leica Fluorescence microscope (Leica Microsystems). Images were analyzed by ImageJ software.
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Targeting Noradrenergic Neurons of the Locus Coeruleus: A Comparison of Model Systems and StrategiesbioRxiv - Neuroscience 2022Quote: ... After fixation brains were embedded in 3% agarose (Genaxxon Bioscience) and coronal slices of ~50 μm thickness were obtained using a vibratome (VT 1000S, Leica). Slices were stored in PBS at 4°C until they were stained and mounted on microscope slides.
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bioRxiv - Neuroscience 2021Quote: ... the pituitaries were embedded in 3% agarose (H2O) and para-sagittally sectioned with 60 μm thickness using a vibratome (Leica). From a single pituitary ...
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bioRxiv - Biochemistry 2020Quote: ... Protein mixture (5 μL) were deposited onto the grids and blotted for 3 seconds using an Automatic Plunge Freezer (Leica).
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bioRxiv - Immunology 2021Quote: ... The fixed specimens were embedded directly in paraffin blocks and cut into sections with 3–5 μm thickness using a rotary microtome (Leica RM 2125RTS, Leica Biosystems Nussloch GmbH ...
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bioRxiv - Immunology 2021Quote: ... The fixed specimens were embedded directly in paraffin blocks and cut into sections with 3–5 μm thickness using a rotary microtome (Leica RM 2125RTS ...
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bioRxiv - Microbiology 2022Quote: ... the cell pellet was spread on the 200-μm side of a 3-mm type A gold plate (Leica Microsystems) covered with the flat side of a 3-mm type B aluminum plate (Leica Microsystems ...
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bioRxiv - Cell Biology 2020Quote: ... embedded in paraffin and sectioned into 3-μm thick slices using a semi-automated rotary microtome (Leica Biosystems, Wetzlar, Germany). The slices were floated in a 37 °C water bath and mounted either on frosted-edge slides for histological staining (Hurst Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... Images were acquired by confocal microscopy using a Leica SP5 system controlled by the LAS AF 3 software (Leica Microsystems). For semi-quantitative quantification of the fluorescence intensity ...
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bioRxiv - Genetics 2021Quote: Assessment of Aβ deposition was carried out by immunohistochemical staining of paraffin-embedded sections (3–5 μm-thick) with a mouse monoclonal (S6 F/3D) anti-Aβ antibody (Leica Biosystems Newcastle Ltd ...
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bioRxiv - Immunology 2021Quote: ... After fixation organs were stored in PBS till paraffin embedment and cut of serial sections on a microtome (2-3 µm; Leica RM 2035 ...
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bioRxiv - Molecular Biology 2022Quote: ... and 3-8 randomly chosen fields per view per Matrigel assay were acquired using confocal Z-stack imaging (TCS-SP8; Leica). Attached pericytes were counted by using the LASX Software.
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bioRxiv - Cell Biology 2023Quote: ... Tissues from at least 3 independent mice were imaged using an SP5X laser scanning confocal microscope (Leica Microsystems CMS GmbH) or an LSM980 microscope and ZEN Desk 3.4 software (Carl Zeiss GmbH) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The growth culture medium was changed every 2-3 days and cell morphology was regularly checked by visual inspection with phase-contrast microscopy (Leica DMIC ...
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bioRxiv - Developmental Biology 2023Quote: ... was injected into the cytoplasm of wild-type embryos at one-cell stage using an MPPI-3 microinjector (ASI) under a stereomicroscope (S9E, Leica). Injected embryos were incubated in the embryo medium (10% Hank’s with full strength calcium and magnesium ...
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bioRxiv - Neuroscience 2023Quote: ... Tissue blocks of the substantia nigra were then embedded in 3% agarose and sectioned using a vibratome (Leica, VT1000S, Germany).
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bioRxiv - Bioengineering 2024Quote: ... Samples were embedded within a 3% agarose (BP160-100, Fisher Bioreagents) mixture and sectioned in a vibratome (VT 1200, Leica) into 300-µm thick sections ...
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bioRxiv - Bioengineering 2024Quote: ... z-stacks were taken at a minimum of 3 places on the film and post-processed with Thunder image analysis (Leica) to remove background fluorescence.
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bioRxiv - Developmental Biology 2024Quote: ... Live VL3/VL4 NMJs in abdominal hemi-segment 3 were imaged in ambient temperature using a Stellaris 5 laser-scanning confocal microscope (Leica) with a water-immersion objective (HC FLUOTAR L VISIR 25x/0.95 ...
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bioRxiv - Immunology 2023Quote: ... A total volume of 3 µl was used for plunge freezing into liquid ethane using an automatic plunge freezer EM GP2 (Leica). The ethane temperature was set to −183 °C and the chamber to 24 °C with 80% humidity ...
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bioRxiv - Neuroscience 2023Quote: ... Brains were post-fixed 24h in 4% PFA before being cut coronally and serially (10 series of 40 µm sections or 3 series of 100 µm sections for morphological studies) with a vibratome (Leica).
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bioRxiv - Neuroscience 2023Quote: ... which were subsequently blotted for 3 seconds and vitrified by plunging into liquid ethane using an EM-GP2 plunge-freezer (Leica). Grids were then stored in liquid nitrogen until data collection.
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bioRxiv - Bioengineering 2023Quote: ... z-stacks were taken at a minimum of 3 places on the film and post-processed with Thunder image analysis (Leica) to remove background fluorescence.
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bioRxiv - Neuroscience 2023Quote: ... fixed brains were embedded in 3% agarose and sectioned at 50 μm thickness with a vibrating microtome (Leica VT1000S Microtome). Free floating sections were incubated in 1% hydrogen peroxide for 15 minutes then incubated with anti-ATP synthase subunit C (Abcam ...
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bioRxiv - Neuroscience 2023Quote: ... Brains were either embedded in 3% agarose for coronal sectioning at 150 µm thickness on a vibrating microtome (VT1000S; Leica) or cryoprotected samples and sectioned coronally at 20 µm on a Leica CM3050 cryostat ...
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bioRxiv - Neuroscience 2022Quote: ... embedded into 3% low melting point agarose and then sectioned into 50-60 um using a vibratome (Leica, cat# VT1000S), mounted for direct visualization of EGFP or processed for immunofluorescence as it follows ...
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bioRxiv - Microbiology 2022Quote: ... Grids were post-stained for 30 min with 2% uranyl acetate (Roth) and 20 min with 3% lead citrate (Leica). TEM images were acquired using a Zeiss Leo 912 Omega (Zeiss ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... the lamps were positioned above zebrafish embryos in 6-well plates such that the power of UV irradiation was ∼5 mW/ cm2 [as measured by a hand-held power sensor (Spectroline, XDS-1000] and ∼3 mW/cm2 for cells. For all imaging experiments, a Leica M205-FA microscope equipped with a stereomicroscope was used ...
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bioRxiv - Neuroscience 2022Quote: ... the tissue was embedded in 3% agarose (in H2O) and sectioned parasagitally with 70 μm thickness using a vibratome (Leica). The slices were mounted using Vectashield H-1000 Mounting Medium (Vector ...
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bioRxiv - Plant Biology 2022Quote: ... 5-day-old wild-type Arabidopsis roots or 3-week-old true leaves were frozen in a high-pressure freezer (EM ICE, Leica), followed by freeze substitution in dry acetone containing 0.4% uranyl acetate at −85°C in a freeze-substitution unit (EM AFS2 ...
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bioRxiv - Bioengineering 2023Quote: ... z-stacks were taken at a minimum of 3 places on the film and post-processed with Thunder image analysis (Leica) to remove background fluorescence.
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bioRxiv - Cell Biology 2024Quote: ... Zeiss) equipped with a 100× /1.4 NA objective lens and a super-resolution confocal microscope (Leica TCS SP8 STED × 3) equipped with a 100× /1.4 NA objective lens were used for cell observation ...
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bioRxiv - Biophysics 2024Quote: ... Grids were blotted for 3-5 seconds then plunge frozen into liquid ethane cooled by liquid nitrogen using an EM GP2 (Leica) plunge freezer at 4°C and 100% relative humidity ...
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bioRxiv - Immunology 2024Quote: ... Images were taken using a Leica Inverted 3 TCS SP8 DMi8 and images were processed with Leica Application Suite (LAS) AF Lite software (Leica).
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bioRxiv - Microbiology 2024Quote: ... the eYFP fluorescence of fusion proteins was analyzed 2–3 days after infiltration using a confocal microscope (Leica TCS SP8).
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bioRxiv - Cell Biology 2024Quote: ... the tissue was embedded in 3% agarose and cut into 200 µm thick slices using a vibratome (VT1200 S, Leica). The slices were optically cleared overnight ...
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bioRxiv - Molecular Biology 2022Quote: ... Green siliques were harvested 12 d after flowering from 4-6 week old plants and opened using fine needles under a dissecting microscope (Leica MZ9.5, Germany). Images of opened siliques were captured using a Leica DFC290 (Germany ...
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bioRxiv - Cell Biology 2023Quote: ... Dried samples were then rotary shadowed with 2 nm of platinum (sputtering) and 4-6 nm of carbon (carbon thread evaporation) using an ACE600 metal coater (Leica Microsystems, Germany). The resultant platinum replica was floated off the glass with hydrofluoric acid (5%) ...
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bioRxiv - Microbiology 2024Quote: ... containing 4‘,6-diamidino-2-phenylindole (DAPI) and imaged as midsections by confocal laser scanning microscopy (Leica SP8: Leica Microsystems, Wetzlar, Germany). To prevent cross talk between the channels for the different fluorochromes ...
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bioRxiv - Neuroscience 2023Quote: ... Images of cultured ependymocytes co-treated with Sir-Actin and calcein AM were captured at 10-s intervals for 12 minutes while that of co-treated with Sir-Actin and fluo-4 AM were captured at 10-s intervals for 6 minutes using confocal scanning microscope (DMi8, Leica, Wetzlar, Germany). The intensity of F-actin area from all Sir-Actin positive cells in a field of recording were included in the statistics ...
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bioRxiv - Neuroscience 2023Quote: ... The fluorescence signals of Fluo-4 were monitored at 10-s intervals for 6 minutes by confocal scanning microscope (DMi8, Leica, Wetzlar, Germany). As previously described 46 ...
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bioRxiv - Cell Biology 2022Quote: ... Dried samples were then rotary shadowed with 2 nm of platinum and 4-6 nm of carbon using the ACE600 (Leica Microsystems, Germany). Areas of interest from the resulting platinum replicas were separated from glass with 5% hydrofluoric acid and deposited on carbon-coated EM grids (200 mesh formvar/carbon ...
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bioRxiv - Cell Biology 2022Quote: ... Dried samples were then rotary shadowed with 2 nm of platinum (sputtering) and 4-6 nm of carbon (carbon thread evaporation) using an ACE600 metal coater (Leica Microsystems, Germany). The resultant platinum replica was floated off the glass with hydrofluoric acid (5%) ...