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Citations for Leica :
201 - 250 of 1586 citations for 2' 5' Dichloro 3 3 4 dimethylphenyl propiophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... Grids were blotted between 2.5 and 3 sec and plunged frozen in liquid ethane using a Leica vitrification plunger (EM GP2, Leica Microsystems). For RiboRad6mix ...
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bioRxiv - Biochemistry 2022Quote: ... The cells were fixed with 3% formaldehyde and permeabilized with 0.1% Triton X-100 and imaged in Leica Fluorescence microscope (Leica Microsystems). Images were analyzed by ImageJ software.
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Targeting Noradrenergic Neurons of the Locus Coeruleus: A Comparison of Model Systems and StrategiesbioRxiv - Neuroscience 2022Quote: ... After fixation brains were embedded in 3% agarose (Genaxxon Bioscience) and coronal slices of ~50 μm thickness were obtained using a vibratome (VT 1000S, Leica). Slices were stored in PBS at 4°C until they were stained and mounted on microscope slides.
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bioRxiv - Neuroscience 2021Quote: ... the pituitaries were embedded in 3% agarose (H2O) and para-sagittally sectioned with 60 μm thickness using a vibratome (Leica). From a single pituitary ...
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bioRxiv - Microbiology 2022Quote: ... the cell pellet was spread on the 200-μm side of a 3-mm type A gold plate (Leica Microsystems) covered with the flat side of a 3-mm type B aluminum plate (Leica Microsystems ...
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bioRxiv - Cell Biology 2020Quote: ... embedded in paraffin and sectioned into 3-μm thick slices using a semi-automated rotary microtome (Leica Biosystems, Wetzlar, Germany). The slices were floated in a 37 °C water bath and mounted either on frosted-edge slides for histological staining (Hurst Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... Images were acquired by confocal microscopy using a Leica SP5 system controlled by the LAS AF 3 software (Leica Microsystems). For semi-quantitative quantification of the fluorescence intensity ...
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bioRxiv - Neuroscience 2022Quote: ... embedded into 3% low melting point agarose and then sectioned into 50-60 um using a vibratome (Leica, cat# VT1000S), mounted for direct visualization of EGFP or processed for immunofluorescence as it follows ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... the lamps were positioned above zebrafish embryos in 6-well plates such that the power of UV irradiation was ∼5 mW/ cm2 [as measured by a hand-held power sensor (Spectroline, XDS-1000] and ∼3 mW/cm2 for cells. For all imaging experiments, a Leica M205-FA microscope equipped with a stereomicroscope was used ...
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bioRxiv - Neuroscience 2022Quote: ... the tissue was embedded in 3% agarose (in H2O) and sectioned parasagitally with 70 μm thickness using a vibratome (Leica). The slices were mounted using Vectashield H-1000 Mounting Medium (Vector ...
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bioRxiv - Plant Biology 2022Quote: ... 5-day-old wild-type Arabidopsis roots or 3-week-old true leaves were frozen in a high-pressure freezer (EM ICE, Leica), followed by freeze substitution in dry acetone containing 0.4% uranyl acetate at −85°C in a freeze-substitution unit (EM AFS2 ...
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bioRxiv - Bioengineering 2024Quote: ... Samples were embedded within a 3% agarose (BP160-100, Fisher Bioreagents) mixture and sectioned in a vibratome (VT 1200, Leica) into 300-µm thick sections ...
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bioRxiv - Bioengineering 2024Quote: ... z-stacks were taken at a minimum of 3 places on the film and post-processed with Thunder image analysis (Leica) to remove background fluorescence.
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bioRxiv - Molecular Biology 2022Quote: ... and 3-8 randomly chosen fields per view per Matrigel assay were acquired using confocal Z-stack imaging (TCS-SP8; Leica). Attached pericytes were counted by using the LASX Software.
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bioRxiv - Cell Biology 2023Quote: ... Tissues from at least 3 independent mice were imaged using an SP5X laser scanning confocal microscope (Leica Microsystems CMS GmbH) or an LSM980 microscope and ZEN Desk 3.4 software (Carl Zeiss GmbH) ...
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bioRxiv - Neuroscience 2023Quote: ... until the brains sank in the solution (1-3 days) and at which point 35um thick hypothalamic sections were obtained using a cryostat (Leica). Sections were first incubated for 1 hour in blocking buffer (1X PBS with 2% BSA and 0.1% tween-20) ...
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bioRxiv - Bioengineering 2023Quote: ... z-stacks were taken at a minimum of 3 places on the film and post-processed with Thunder image analysis (Leica) to remove background fluorescence.
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bioRxiv - Neuroscience 2023Quote: ... fixed brains were embedded in 3% agarose and sectioned at 50 μm thickness with a vibrating microtome (Leica VT1000S Microtome). Free floating sections were incubated in 1% hydrogen peroxide for 15 minutes then incubated with anti-ATP synthase subunit C (Abcam ...
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bioRxiv - Developmental Biology 2023Quote: ... was injected into the cytoplasm of wild-type embryos at one-cell stage using an MPPI-3 microinjector (ASI) under a stereomicroscope (S9E, Leica). Injected embryos were incubated in the embryo medium (10% Hank’s with full strength calcium and magnesium ...
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bioRxiv - Neuroscience 2023Quote: ... Brains were post-fixed 24h in 4% PFA before being cut coronally and serially (10 series of 40 µm sections or 3 series of 100 µm sections for morphological studies) with a vibratome (Leica).
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bioRxiv - Neuroscience 2023Quote: ... which were subsequently blotted for 3 seconds and vitrified by plunging into liquid ethane using an EM-GP2 plunge-freezer (Leica). Grids were then stored in liquid nitrogen until data collection.
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bioRxiv - Immunology 2023Quote: ... A total volume of 3 µl was used for plunge freezing into liquid ethane using an automatic plunge freezer EM GP2 (Leica). The ethane temperature was set to −183 °C and the chamber to 24 °C with 80% humidity ...
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bioRxiv - Bioengineering 2023Quote: ... z-stacks were taken at a minimum of 3 places on the film and post-processed with Thunder image analysis (Leica) to remove background fluorescence.
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bioRxiv - Neuroscience 2023Quote: ... Brains were either embedded in 3% agarose for coronal sectioning at 150 µm thickness on a vibrating microtome (VT1000S; Leica) or cryoprotected samples and sectioned coronally at 20 µm on a Leica CM3050 cryostat ...
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bioRxiv - Neuroscience 2023Quote: ... Tissue blocks of the substantia nigra were then embedded in 3% agarose and sectioned using a vibratome (Leica, VT1000S, Germany).
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bioRxiv - Cell Biology 2024Quote: ... Zeiss) equipped with a 100× /1.4 NA objective lens and a super-resolution confocal microscope (Leica TCS SP8 STED × 3) equipped with a 100× /1.4 NA objective lens were used for cell observation ...
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bioRxiv - Immunology 2024Quote: ... Images were taken using a Leica Inverted 3 TCS SP8 DMi8 and images were processed with Leica Application Suite (LAS) AF Lite software (Leica).
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bioRxiv - Cell Biology 2024Quote: ... the tissue was embedded in 3% agarose and cut into 200 µm thick slices using a vibratome (VT1200 S, Leica). The slices were optically cleared overnight ...
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Recruitment of MRE-11 to complex DNA damage is modulated by meiosis-specific chromosome organizationbioRxiv - Genetics 2020Quote: ... Figure 4 and Figure S3): 5-10 adult worms were placed on an uncharged slide (Surgipath Leica) in 5µl M9 solution ...
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bioRxiv - Biophysics 2024Quote: ... After blotting for 4-5 s in a Leica EM GP2 plunge freezer (Leica Microsystems, Wetzlar, Germany) with the chamber set to 5°C and 95% humidity ...
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bioRxiv - Biophysics 2021Quote: ... These dauer samples were then transferred to carriers of 3 mm diameter and 0.1 mm depth and rapidly frozen in a high-pressure freezing machine (Leica, EM ICE). For automated freeze substitution ...
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bioRxiv - Neuroscience 2021Quote: ... i.p.) at P120 and P123 (after 3 days of monocular deprivation) Images were visualized using a custom Leica microscope (Leica Microsystems). Red light illumination was provided by 8 individually addressable LEDs (WS2812 ...
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bioRxiv - Cell Biology 2021Quote: ... was assembled on a Narishige micromanipulator model MM-3 (Narishige group) and was placed on the magnetic board of a Leica MS5 dissection microscope (Leica Biosystems), using a Narishige GJ-8 magnetic stand (Narishige group) ...
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bioRxiv - Neuroscience 2020Quote: ... Mice were anesthetized with a mixture of oxygen (1 L/min) and isoflurane 3% (Baxter AG, Vienna, Austria) and placed in a stereotactic frame (Angle One; Leica, Germany). The skin was shaved ...
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bioRxiv - Plant Biology 2020Quote: ... and germlings was measured after 15 min of exposure to NCR044.1 (50 μL, 3 μM) using fluorescence confocal microscopy (Leica SP8-X) with an excitation and emission wavelength of 488 and 508-544 nm ...
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bioRxiv - Cell Biology 2021Quote: ... around 1.5 µl of concentrated purified schizont pellet was transferred into aluminium or gold carriers (3 mm diameter, 100 or 200 µm depth; Leica Microsystems) and high pressure frozen with EM ice (Leica Microsystems) ...
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bioRxiv - Neuroscience 2020Quote: ... Brains were embedded in agarose (3% in Water) and 70 µm coronal sections were cut with a VT1000S vibratome (Leica Biosystems). Every second section ...
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bioRxiv - Neuroscience 2021Quote: ... Mice were anesthetized with a mixture of oxygen (1L/min) and isoflurane 3% (Baxter AG, Vienna, Austria) and placed in a stereotactic frame (Angle One; Leica, Germany). The skin was shaved ...
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bioRxiv - Physiology 2020Quote: ... Finally, sections were incubated with DAB (3,3’-diaminobenzidine, Vector) for 3 min and counterstained with Gill’s hematoxylin (Leica, Buffalo Grove, IL) for 30 sec ...
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bioRxiv - Microbiology 2021Quote: ... containing defined regions of the bacterial colonies were cut out and inverted on lecithin-coated 3-mm specimen carriers for high pressure freezing in a high- pressure freezing machine (Leica, ICE) with or without 10% polyvinylpyrrolidone as a filler (Δeps biofilms and one of the WT samples were frozen without filler) ...
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bioRxiv - Cell Biology 2021Quote: ... microscopy was performed with the 100×/1.4 Oil HC PL APO CS2 STED White objective of a Leica TCS SP8 3× STED microscope (Leica, Wetzlar, Germany) equipped with a white light laser ...
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bioRxiv - Neuroscience 2022Quote: ... Brains were post-fixed for 1–3 days and sectioned coronally (50–100 μm in thickness) using a vibratome (Leica; VT1000S). Brain sections were mounted on superfrost slides and coverslipped with ProLong antifade reagent with DAPI (Molecular Probes) ...
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bioRxiv - Plant Biology 2022Quote: ... were infiltrated in Nicotiana benthamiana leaves for transient expression and viewed after 3 days under confocal microscope Leica TCS SP5 (Leica Microsystems) equipped with appropriate lasers (514 nm -527 nm and 532 nm-588 nm for YFP and RFP ...
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bioRxiv - Molecular Biology 2023Quote: ... Image acquisition was performed as z-stack images of 250 μm in depth in 3 separate channels using a Leica SP8 confocal microscope (Leica, Germany).
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bioRxiv - Cell Biology 2023Quote: ... The paraffin embedded clots were cut in 3 µm thick sections with a semi-automated rotary microtome (Leica Biosystems, Wetzlar, Germany) and placed on silanized glass slides ...
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bioRxiv - Neuroscience 2023Quote: ... cluster of differentiation 3 (CD3) and CD68 immunostaining was performed using a fully automated staining system (BOND-III; Leica Biosystems, UK). In brief ...
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bioRxiv - Neuroscience 2023Quote: ... Coronal sections of 3 µm thickness were cut at the level of the hippocampus using a microtome (Leica Microsystems, Wetzlar, Germany). For immunohistochemistry either paraffin sections that were washed with 0.5% sodium azide (2 × 5 min ...
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bioRxiv - Cell Biology 2023Quote: ... followed by 1h incubation in the same buffer containing the primary probes and a 3 min heat incubation step in a ThermoBrite slide hybridization system (Leica Biosystems), either at 75°C or 86°C ...
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bioRxiv - Biophysics 2023Quote: ... and then glued onto Leit-tabs (Plano, Germany) mounted on aluminum stubs (Plano, Germany) and sputter-coated with a 3 nm thin gold layer (ACD600, Leica, Austria). SEM images were acquired with a Zeiss Auriga FEG-SEM (Zeiss ...
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bioRxiv - Microbiology 2024Quote: ... Cells were washed 3 times with PBS and imaged using a Leica SP8 laser scanning confocal microscope (Leica Microsystems, Heidelberg, Germany) using a 63x water objective ...