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Citations for Leica :
151 - 188 of 188 citations for L Asparagine H2O 13C4; D3; 15N2 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: Immunostained samples were acquired by confocal and 2-photon microscopy using the same Leica SP8 and a coverslip corrected HCX IRAPO L 25X/0.95NA water immersion objective (Leica Microsystems). Alexa Fluor 488 and Alexa Fluor 594 were excited with lasers emitting at 488 nm and 552 nm wavelengths respectively ...
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bioRxiv - Microbiology 2023Quote: ... islandicus REY15A grown under poor media (4.5 μl) was applied to glow-discharged lacey carbon grids and then plunge-frozen using an EM GP Plunge Freezer (Leica). The cryo-EMs were collected on a 300 keV Titan Krios with a K3 camera at 1.08 Å per pixel and a total dose of 50 e-/Å2 ...
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bioRxiv - Plant Biology 2024Quote: ... Each 3 µl of protein reaction mix was loaded into a flow-chamber and immediately observed with a confocal microscope SP5 (Leica) equipped with a 60x oil objective (NA 1.42) ...
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bioRxiv - Immunology 2024Quote: ... The imaging was performed in regular confocal mode using long-working distance objective (L HC FLUOTAR 25x, NA=0.95, Leica Microsystems). To visualize blood vessels in the liver ...
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bioRxiv - Cancer Biology 2024Quote: ... The cells were then washed three times with 1X PBS and incubated with geminin-L-CE primary antibody (Leica Biosystems) for 1 hour ...
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bioRxiv - Cancer Biology 2021Quote: ... Tissue clearing was performed using the FluoClearBABB approach24 and whole mount images were then acquired using a SP8 microscope equipped with a white light laser and a HCX APO L 20x/0.95 IMM lens (Leica Microsystems, Germany). Imaging data was analyzed on a workstation (Thinkmate ...
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bioRxiv - Immunology 2021Quote: ... Six consecutive staining cycles were performed using the following 1ry Antibody-Opal fluorophore pairings: CD4 (clone 4B12, NCL-L-CD4-368 (Leica Novocastra) – Opal 520) ...
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bioRxiv - Neuroscience 2022Quote: ... osmolarity 280-320 mOsm/l) was used both to keep tissue cold during dissection and as the bathing solution during vibratome slicing (Leica VT1200S). 0.4-1 mm-thick dorsal OB slices were taken by near-horizontal slicing ...
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bioRxiv - Neuroscience 2020Quote: ... Mice were anesthetized with a mixture of oxygen (1 L/min) and isoflurane 3% (Baxter AG, Vienna, Austria) and placed in a stereotactic frame (Angle One; Leica, Germany). The skin was shaved ...
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bioRxiv - Physiology 2021Quote: ... 20 larvae per tank were anaesthetized with MS-222 (50 mg l-1, Pharma Q) prior to feeding and directly photographed individually with a microscope (Leica M165C). The larvae were then frozen in liquid nitrogen and stored at −80°C until dry mass (DM ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Incubation with primary antibodies diluted in blocking buffer was carried out at 4°C from 2 hours to overnight for the mouse anti-α-SG 1:100 (NCL-L-a-SARC, Novocastra, Leica), the rabbit anti-HSP90 1:1000 (4877S ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... for blocking (1 hour, room temperature) and with a mouse anti-α-SG 1:100 (NCL-L-a-SARC, Novocastra, Leica) or a mouse anti-γ-SG 1:100 (NCL-L-g-SARC ...
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bioRxiv - Neuroscience 2022Quote: ... mice were anesthetized with a mixture of oxygen (1 L/min) and isoflurane 2% (Baxter AG, Vienna, Austria) and placed in a stereotactic frame (Angle One; Leica, Germany). The skin was locally anesthetized with 40– 50 μL lidocaine 0.5% and disinfected with betadine.
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bioRxiv - Plant Biology 2024Quote: ... The leaf sections were then mounted with Polyvinyl Alcohol-Lactic acid (PVA-L) medium (8% v/v) microscopy glue and observed under light microscope (Leica DM5500B). Pycnidia width was calculated using the custom scale bar in Leica DM5500B microscope ...
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bioRxiv - Cancer Biology 2024Quote: We accessed a TNBC data from a previous study in which mouse anti-human monoclonal AR primary antibody (AR-318-L-CE, Leica Biosystems) was used to detect AR in a tissue microarray (TMA ...
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bioRxiv - Cancer Biology 2024Quote: ... Primary antibodies were incubated overnight at 4°C with the following dilutions in blocking buffer: p53 (CM5) (1:300, NCL-L-p53-CM5p, Leica Biosystems), Cleaved Caspase-3 (Asp175 ...
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bioRxiv - Developmental Biology 2024Quote: Adult zebrafish were anesthetized in buffered tricaine (160 mg/L) and placed on a moist paper towel under a dissecting microscope (Leica M165) with goose neck illumination ...
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bioRxiv - Neuroscience 2022Quote: ... The excitation beam was focused with a water-dipping objective lens (Nikon CFI75 16× 0.8NA or Leica HC FLUOTAR L 25x 0.95NA). The average power was 100-150 mW at the sample ...
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bioRxiv - Plant Biology 2020Quote: ... containing 1 g/l calcofluor white M2R and 0.5 g/l evans blue and then mounted on a TCS SP5 II CLSM (Leica Microsystems, Vienna, Austria). As emission source a 405 nm UV diode was used ...
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bioRxiv - Biochemistry 2021Quote: ... 4 μL sample were applied onto a grid held in the sample grid chamber of a Leica EM GP instrument (Leica Microsystem Inc.) cooled at 4 °C ...
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bioRxiv - Plant Biology 2023Quote: ... T1 seeds from plants transformed with pSS01_35Spro:LIP1-GFP and pB7WG2_35Spro:LIP1 were harvested and selected either with 240 mg/L glufosinate ammonium solution (Basta) or screened for fluorescence on a stereomicroscope (Leica M165 FC) equipped with a GFP filter (excitation ...
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bioRxiv - Cancer Biology 2024Quote: The assay consisted of the following antibodies and corresponding dilution factor: CK AE1/AE3 (Leica Biosystems, ref. AE1/AE3-601-L-CE) was applied at 1:250 for 28 minutes at 37°C ...
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bioRxiv - Bioengineering 2024Quote: ... applied onto glass coverslips precoated by poly-L-lysine and immediately analyzed by a Leica TCS SP8 confocal microscope (Leica, Wetzlar, Germany) equipped with a water immersion objective HC PL APO CS2 with magnification 63x ...
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bioRxiv - Plant Biology 2024Quote: ... a 1-μl sample was diluted in a 1:10 ratio with 10% (w/v) sucrose and observed under a stereomicroscope (Leica, M165 FC). Then ...
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bioRxiv - Cancer Biology 2024Quote: Confirmation of proliferating cells and cell death were assessed by IF of Ki-67 (Leica, Cat# KI67-MM1-L-CE, 1:300) and DeadEnd Fluorometric TUNEL assay (Promega ...
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bioRxiv - Cancer Biology 2021Quote: Formalin-fixed paraffin-embedded (FFPE) human skin tissue sections from organotypic tissue was stained for MITF (NCL-L-MITF, Leica Biosystems, Nussloch, Germany), MelanA (NCL-L-MITF ...
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bioRxiv - Neuroscience 2021Quote: ... Red and cyan fluorescence were detected through the objective (Olympus LUMPLFLN 60XW, 1.0 NA, or Leica HC FLUOTAR L 25x/0.95 W VISIR) and through the oil immersion condenser (numerical aperture 1.4 ...
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bioRxiv - Microbiology 2022Quote: ... Aliquots of 3 μl were added to the plasma cleaned grids and subsequently plunge-frozen in liquid ethane using a Leica EMGP (Leica microsystems, Wetzlar, Germany). Blotting time was set to 1s with the chamber temperature and humidity being at 20°C and 85% ...
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bioRxiv - Cancer Biology 2024Quote: ... in TBST buffer for IF and subjected to staining with the following antibodies overnight at 4C: p53 (Leica, P53-CM5P-L 1:100), GFP/YFP (Abcam ...
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bioRxiv - Neuroscience 2020Quote: ... iAChSnFR fluorescence was detected by a photomultiplier tube (H11706-40, Hamamatsu) through a 25x objective lens (Leica, HCX IRAPO L 25x/ 0.95 W, Germany), a dichroic mirror (Semrock) ...
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bioRxiv - Plant Biology 2023Quote: ... 5 μl was transferred into an HPF flat specimen carrier and frozen with a Leica EM PACT high-pressure freezer (Leica Microsystems, Inc., Bannockburn, IL) at a typical rate of 1,700°C/s ...
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bioRxiv - Neuroscience 2022Quote: ... 0.4 mM L-ascorbic acid and the pH was adjusted to 7.4) and then sliced on a vibratome (Leica, VT1200 S, Deerfield, IL, USA). Brain slices (300 μm thick ...
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bioRxiv - Neuroscience 2023Quote: ... Lewy bodies were counted on sections from the described regions following immunohistochemistry for alpha-synuclein (Leica, cat# ncl-l-asyn, 1:100-400 dilution). Of note ...
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bioRxiv - Molecular Biology 2023Quote: ... Zi-1 = 75.61μm) of pancreatic beta cells using the microscope’s TXR filter and a 40X objective (Leica HCX APO L U-V-I 0.80 W), at 20 msec exposures ...
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bioRxiv - Pathology 2023Quote: ... Diluted primary antibody (CST #8242 1:800; CST #99940, 1:150; CST #9718, 1:500; Abcam ab107099, 1:X; Leica P53-CM5P-L 1:200) was applied and slides were incubated at 4°C overnight ...
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bioRxiv - Molecular Biology 2023Quote: ... three z-stacks of 75 optical sections each (ΔZi, Zi-1 = 151.75μm) were acquired of the bigger liver lobe using a 20X objective (Leica HCX APO L U-V-I 0.80 W). One z-stack was in bright field and acquired at 20 msec exposures ...
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bioRxiv - Developmental Biology 2024Quote: ... We then imaged the exposed region of the midgut us-ing an upright Leica SP5 multi-photon confocal microscope with a 20x water immersion objective (Leica HCX APO L 20x NA 1.0). We fed flies via a microcapillary feeder tube throughout the entire imaging process ...
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bioRxiv - Neuroscience 2021Quote: Cerebral vascular pulsation was assessed through the cortical vascular network with the use of multiphoton microscopy (LaVision Biotech TriMScope with Nikon CFI-Apo 25x NA1.1 lens and Leica SP8 DIVE with IRAPO L 25x NA1.0 lens) using a method based on those described previously within the literature (Iliff et al. ...