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Citations for Leica :
151 - 200 of 237 citations for H Leu arg pro gly nh2 2hcl since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... and stained with either H&E or specific immunohistochemistry markers (HA-Tag C29F4 #3724 from Cell Signaling Technology; GranzymeB Leica Biosystems PA0291 ...
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bioRxiv - Plant Biology 2023Quote: ... GFP signals were detected at different time intervals between 48-96 h post infiltration (hpi) with the TCS SP8 microscope (Leica).
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bioRxiv - Genetics 2024Quote: ... Sections of TA and liver were stained with hematoxylin and eosin (H&E) using an XL CV5030 autostainer from Leica. Liver cryosections were stained with Oil Red-O and counterstained with hematoxylin using established protocols ...
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bioRxiv - Molecular Biology 2024Quote: ... standard Hematoxylin and Eosin (H&E) staining was performed using a Leica Autostainer XL (Leica Biosystems, Buffalo Grove, IL, USA). Tissue sections were cleared with Xylene (Mercedes Scientific ...
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bioRxiv - Immunology 2024Quote: ... Paraffin-embedded sections of the lungs were subjected to H&E staining and then examined by Thunder 3D Cell Culture (Leica).
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bioRxiv - Immunology 2024Quote: ... Slides were stained following standard hematoxylin and eosin (H&E) staining procedures (Hematoxylin: 3801561, Leica; Bluing Reagent: 6769001, Epredia; Eosin: 3801601, Leica). Samples were cleared using xylene and coverslips were mounted with Permount Mounting Media (SP15-100 ...
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bioRxiv - Neuroscience 2024Quote: ... brains were transferred into 30 % sucrose (w/v) in PBS for 48 h and frozen in Tissue Freezing Medium (Leica) at −80°C.
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bioRxiv - Cancer Biology 2024Quote: ... the slides were stained with hematoxylin and eosin (H&E) using a standard protocol and digitally imaged using the Aperio ScanScope (Leica).
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bioRxiv - Cell Biology 2024Quote: Mice lung specimens were fixed in 4% paraformaldehyde for 48 h and subsequently embedded in paraffin using ASP200S and EG1150H (Leica). At the time of death ...
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bioRxiv - Cell Biology 2020Quote: ... Automated freeze-substitution in 0.1% uranyl acetate in acetone over a 72 h period and embedding in Lowicryl HM20 resin were performed using an EM AFS2 (Leica Microsystems) as previously described (Kukulski et al. ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were embedded at 60°C for 48 h and cut into 70 nm sections with a microtome (EM UC7, Leica, Germany). After electron staining with uranyl acetate and lead citrate ...
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bioRxiv - Molecular Biology 2020Quote: Cells expressing EGFP protein were visualized 48 h post-transfection by fluorescent microscopy using Leica DMi8 inverted microscope (Leica Microsystems, Germany) at magnification 10x ...
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bioRxiv - Molecular Biology 2021Quote: ... Sections of TA and liver were stained with hematoxylin and eosin (H&E) using an XL CV5030 autostainer (Leica, Wertzler, Germany). Samples stained with H&E were scanned with a MIRAX MIDI digital slide scanner (Zeiss ...
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bioRxiv - Microbiology 2022Quote: ... and were stained with hematoxylin and eosin (H&E) and coverslipped using a ST5010-CV5030 stainer/coverslipper combination (Leica, Wetzlar, Germany). Slides were examined blinded by a board-certified veterinary pathologist (N.A.C.) ...
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bioRxiv - Biochemistry 2022Quote: ... 36 h post-transfection both the cell lines were trysinised and co-cultured in 1:1 ratio for 12 h followed by fixing permialization and subsequent imaging using fluorescence microscope (Leica Microsystems) and subsequent analysis by ImageJ software.
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bioRxiv - Microbiology 2022Quote: ... the samples were freeze substituted at −90 °C for 80 h in acetone supplemented with 1% OsO4 and warmed up slowly (1°C h−1) to −60 °C in an automated freeze substitution device (AFS2; Leica Microsystems). After 8 to 12 h ...
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bioRxiv - Bioengineering 2022Quote: ... The gels were subsequently incubated with EM containing 10 μM forskolin (Wako) with DMSO or Inh-172 for 24 h before imaging with a Leica DMi8 (Leica Microsystems) using the Leica Application Suite X (LAS-X ...
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bioRxiv - Cancer Biology 2022Quote: ... spheres were incubated in n-Butanol (three times 30 min) and melted paraffin (three times) before they were Paraffin-embedded using the HistoCore Arcadia H (Leica Biosystems) following the manufacturer’s protocol in a medium size mold ...
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bioRxiv - Neuroscience 2021Quote: ... Brains were harvested and stored in the same solution at 4 °C for 2 h before being sliced into 150 μm-thick slices on a vibratome (Leica VT1000S). The slices were stored in 0.1 M glycine and 0.01% sodium azide in 1× PBS at 4 °C until use.
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bioRxiv - Plant Biology 2020Quote: ... by infiltration for 10 min in a vacuum and after further incubation for 4 h processed using the Leica Semi-Enclosed Benchtop Tissue Processor TP1020 (Leica Biosystems) for an ethanol series (75 % ...
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bioRxiv - Cell Biology 2021Quote: ... The enucleated eyes were further fixed in the same fixative for 24 h and cut on a vibratome (VT1000S, Leica, Germany) at 200μm thickness.
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bioRxiv - Physiology 2021Quote: ... The lung tissue sections of 3-5 micron were stained with H&E stain and were microscopically analyzed using microscope (Make: Leica DM1000 LED; Camera: Leica MC170HD). The images were taken with 10X objective lens (Magnification X 100 ...
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bioRxiv - Biochemistry 2020Quote: ... H&E staining on 10 µm frozen section was performed using the Leica ST5020 Multistainer workstation (Leica Biosystems, Buffalo Grove, IL) according manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... tissues were fixed in neutral buffered formalin (NBF) for 48 h prior to processing using standard protocols in a vacuum infiltration processor (Leica ASP300S). Tissue sections were embedded in paraffin and stained with hematoxylin and eosin (H&E ...
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bioRxiv - Neuroscience 2021Quote: ... post-fixed in 4% PFA for 48 h at 4 °C and cut into 60 μm coronal sections using a vibratome (VT1200S, Leica, Germany). The sections were post-fixed (1 h) ...
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bioRxiv - Plant Biology 2023Quote: ... they were kept at 28±1°C in dark for 16 h and observed under confocal microscope (Leica microsystems, TCS, SP5). BiFC for each combination was repeated twice and appropriate negative controls were used (Kudla and Bock ...
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bioRxiv - Biochemistry 2022Quote: ... H&E staining on 10 μM frozen section was performed using the Leica ST5020 Multistainer workstation (Leica Biosystems, Buffalo Grove, IL) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... The fixed right brain hemisphere tissues were dehydrated using a vacuum infiltration processor (Excelsior ES, Thermo, USA) and embedded in paraffin using an Arcadia H heated embedding workstation (Leica, Germany).
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bioRxiv - Plant Biology 2023Quote: ... Paraplast blocks were then prepared with half seed in each block using the Leica Heated Paraffin Embedding Module EG1150 H with the added Leica Cold Plate for Modular Tissue Embedding System EG1150 C (Leica Biosystems). with vacuum infiltration ...
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bioRxiv - Plant Biology 2023Quote: ... Paraplast blocks were then prepared with dozens of seeds in each block using the Leica Heated Paraffin Embedding Module EG1150 H with the added Leica Cold Plate for Modular Tissue Embedding System EG1150 C (Leica Biosystems) with vacuum infiltration ...
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bioRxiv - Biophysics 2023Quote: 5 µm sections of formalin-fixed and paraffin-embedded (FFPE) mouse tail specimens were H&E-stained by using an automated stainer (Leica XL) at University of Manchester’s Histology Core as previously described [11 ...
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bioRxiv - Cell Biology 2023Quote: ... A 680 nm short pass filter and a 560 nm long pass dichroic mirror were used to respectively reduce the laser scattering and to split the NAD(P)H and FAD fluorescence toward two hybrid photomultiplier detectors (HyD, Leica Microsystems). 460/60 nm ...
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bioRxiv - Molecular Biology 2023Quote: ... The samples were waxed in liquid paraffin for 2 h and embedded to make paraffin blocks using a tissue embedder (EG1150, Leica, Germany). Four-micron sections were cut by paraffin slicer (RM2245 ...
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bioRxiv - Neuroscience 2022Quote: ... Viral titers were determined by transduction of HEK293T cells for 72 h with limiting dilution of MMLV suspension and counting of reporter expressing cell spots under a fluorescent microscope (Leica Microsystems). Titers applied were ~5 × 108 colony-forming units (CFU)/ml (Zhao et al. ...
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bioRxiv - Plant Biology 2022Quote: ... and then incubated for 1 h in a 1:1 solution of 100% ethanol and solution A (Leica Historesin Embedding kit). After 2 h in solution A ...
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bioRxiv - Cell Biology 2022Quote: ... and freeze substituted in anhydrous acetone containing 1% OsO4/2% H2O for 64 h using the Automatic Freeze Substitution unit (AFS; Leica Microsystems). The samples were included in EMbed 812 ...
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bioRxiv - Molecular Biology 2023Quote: ... The samples were waxed in liquid paraffin for 2 h and embedded to make paraffin blocks using a tissue embedder (EG1150, Leica, Germany). Four-micron sections were cut by paraffin slicer (RM2245 ...
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bioRxiv - Neuroscience 2023Quote: ... was cryoprotected with 30% sucrose in PBS 0.1 M for 48 h and cut afterwards in 50-µm-thick coronal sections using a sliding microtome (LEICA SM2000R, Leica). Slices were collected in 6 subseries and stored at −20°C in a cryoprotective solution (30% glycerol ...
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bioRxiv - Bioengineering 2023Quote: ... the sectioned samples were placed on a Hematoxylin and Eosin (H&E) automated staining platform (Leica Auto Stainer XL, Leica Biosystems) without applying any heat during the staining process ...
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bioRxiv - Bioengineering 2023Quote: ... the sectioned samples were placed on a Hematoxylin and Eosin (H&E) automated staining platform (Leica Auto Stainer XL, Leica Biosystems) without applying any heat during the staining process ...
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bioRxiv - Neuroscience 2022Quote: ... timelapse phase contrast images were acquired for 2 h at 37 °C with a 10X or 20X fluotar objective on a Leica DMIRE2 inverted microscope (Leica, Germany) equipped with a MS-2000 XYZ automated stage (ASI ...
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bioRxiv - Pathology 2022Quote: Five micron sections of formalin-fixed and paraffin-embedded (FFPE) specimens were H&E-stained by using an automated stainer (Leica XL) at the University of Manchester Histology Core Facility as previously described [19] ...
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bioRxiv - Microbiology 2023Quote: ... H&E-stained slides were scanned by a whole-slide image technique using an Aperio slide scanner (Leica, Buffalo Grove, IL) with a resolution of 0.24 μm/pixel ...
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bioRxiv - Microbiology 2024Quote: ... the vitrified pellets were freeze-substituted at -90°C for 80 h in acetone supplemented with 2 % OsO4 (AFS2; Leica Microsystems). The samples temperature was increased slowly to -60°C (2°C⋅h-1) ...
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bioRxiv - Immunology 2024Quote: Conventional staining with Hematoxylin Eosin (H&E) was performed with the Stainer Integrated Workstation Leica ST5010-CV5030 (Leica Biosystems, Nussloch, Germany) on 3-micron tissue sections placed on microscope slides according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... post-fixed in 4% PFA for 48 h at 4°C and cut into 100 μm coronal sections using a vibratome (VT1200S, Leica, Germany). The sections were post-fixed (1 h) ...
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bioRxiv - Cancer Biology 2024Quote: ... and baked in a 60°C oven before staining with haematoxylin and eosin (H&E) on the Leica Autostainer X (Leica Biosystems). Immunohistochemistry (IHC ...
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bioRxiv - Cancer Biology 2024Quote: The incidence of metastasis to the liver was determined at study endpoint by analysis of H&E-stained liver sections at 10x magnification on an Aperio ScanScope (Leica Biosystems) in a blinded manner by K.C ...
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bioRxiv - Cancer Biology 2024Quote: ... the slides were stained with hematoxylin and eosin (H&E) using a standard protocol and imaged by brightfield microscopy using the Aperio ScanScope (Leica Biosystems).
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bioRxiv - Molecular Biology 2021Quote: ... Live NIH3T3 and PAC2 cells were imaged 48 h post transfection using a laser scanning confocal microscope (TCS SP8, Leica Microsystems, Germany) with 40x water (N.A ...