Labshake search
Citations for Leica :
151 - 200 of 657 citations for ESM 1 Mouse HEK 293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Developmental Biology 2022Quote: ... 10 μm transverse zebrafish and mouse cryosections were collected on a Leica CM1900 crysostat (Leica Biosystems, Buffalo Grove, IL). 10 μm paraffin mouse sections were collected on a Thermo Scientific Shandon Finesse ME Microtome (Thermo Scientific ...
-
bioRxiv - Neuroscience 2020Quote: ... transverse slices of 150µm from the hippocampi of a P17 mouse were prepared using a vibrating blade microtome (Leica) in cold (4°C ...
-
bioRxiv - Developmental Biology 2020Quote: En face STED images of mouse cephalic explants were taken with a Leica SP8 TCS STED microscope (Leica Microsystems) equipped with a pulsed white-light excitation laser (WLL ...
-
bioRxiv - Cancer Biology 2021Quote: ... A polymer detection system that includes an anti-mouse secondary antibody (DS9800, Novocastra Bond Polymer Refine Detection, Leica Biosystems) was then applied in the tumor samples ...
-
bioRxiv - Cancer Biology 2023Quote: ... automated methods on the Leica Bond Max autostainer using a mouse monoclonal anti-human CD3 antibody (Leica, clone LN10). Whole slide images of IHC stained slides were digitally captured using an Olympus IX83 microscope at 10x magnification and fixed exposure times ...
-
bioRxiv - Microbiology 2023Quote: ... FFPE mouse skin was processed and cut onto a polyethylene naphthalate (PEN) slide designed for LCM processing (Leica 11505158). At least 1,000 SGs or 1,000,000 μm2 of tissue was isolated to obtain enough material for RNA extraction ...
-
bioRxiv - Neuroscience 2023Quote: ... The mouse brains were then cut into 20 µm thick frozen tissue sections using a cryostat (Leica, Cat# CM1950) and placed on Superfrost Plus Adhesion Microscopic glass slides (Epredia ...
-
bioRxiv - Plant Biology 2022Quote: ... and then incubated for 1 h in a 1:1 solution of 100% ethanol and solution A (Leica Historesin Embedding kit). After 2 h in solution A ...
-
bioRxiv - Neuroscience 2019Quote: ... They were then rinsed with PBS and one hemisphere for each mouse was sliced coronally at 100 μm with a vibratome (Leica) for imaging of dTomato-labeled abGC spines (Figure #) ...
-
bioRxiv - Cancer Biology 2022Quote: ... Slides were washed with TBST and incubated with PowerVision Poly-HRP anti-rabbit IgG or anti-mouse IgG (Leica Biosystems) in a humidified chamber at 37°C for 30 minutes ...
-
bioRxiv - Cell Biology 2020Quote: ... Ten pg of the mRNAs was injected into fully grown mouse oocytes using an IM-9B microinjector (Narishige) under a Dmi8 microscope (Leica) in M2 medium containing 10 µM milrinone ...
-
bioRxiv - Cancer Biology 2021Quote: ... for 8 minutes at room temperature (Poly-HRP-mouse anti-rabbit IgG, BOND Polymer Refine Detection System, Leica, Germany; DS9800). A color reaction with 3,3-di-amino-benzidine (DAB chromogen ...
-
bioRxiv - Cancer Biology 2021Quote: ... The slides were incubated with a secondary antibody (rabbit anti-mouse IgG, BOND Polymer Refine Detection System, Leica, Germany, DS9800) for 8 minutes at room temperature ...
-
bioRxiv - Cancer Biology 2021Quote: ... Sections from a series of 17 tumour samples from the validation cohort were stained for CD8 (mouse monoclonal 4B11, Leica Biosystems PA0183 ...
-
bioRxiv - Neuroscience 2020Quote: ... mouse or macaque slices (350 μm) were generated (Compresstome VF-300 vibrating microtome, Precisionary Instruments or VT1200S Vibratome, Leica Biosystems), with a block-face image acquired (Mako G125B PoE camera with custom integrated software ...
-
bioRxiv - Genetics 2021Quote: Assessment of Aβ deposition was carried out by immunohistochemical staining of paraffin-embedded sections (3–5 μm-thick) with a mouse monoclonal (S6 F/3D) anti-Aβ antibody (Leica Biosystems Newcastle Ltd ...
-
bioRxiv - Neuroscience 2022Quote: ... electroporated mouse pups were non-invasively screened at the age of P0 for cortical fluorescence using a fluorescence stereoscope (Leica MZ10f with X-Cite FIRE LED light source ...
-
bioRxiv - Neuroscience 2022Quote: ... The mouse brains were frozen and sectioned sagittally with a thickness of 30 µm on a cryostat (Leica Biosystems, Germany), then the tissue slides were air dried for next step ...
-
bioRxiv - Bioengineering 2022Quote: ... Bio SB) or anti-human CD163 (clone EP324, Bio SB) followed by an HRP-conjugated anti-mouse secondary antibody (Leica) and revealed with the peroxidase substrate 3 ...
-
bioRxiv - Neuroscience 2023Quote: Ambra1 WT and Het female mouse brain were cut into coronal sections (30 μm) with Leica CM1950 instrument (Leica Biosystems). Sections were blocked with 10% normal horse serum (NHS ...
-
bioRxiv - Neuroscience 2023Quote: ... Coronal sections of the perfused mouse brains were cryosectioned into 40μm at −20C using a Leica CM3050 cryostat (Leica Biosystems). In order to detect GFP expression via immunohistochemistry staining ...
-
bioRxiv - Bioengineering 2023Quote: A representative brain from a mouse stimulated with DCZ (mouse 4N with the cranial window) was sectioned into 100 µm slices using a vibrating microtome (Leica). The slices were mounted and stained with DAAPI nuclear stain using ProLong Diamond antifade mountant with DAPI (Thermo Fisher P36962 ...
-
bioRxiv - Cancer Biology 2024Quote: ... Cryo-embedded mouse tumors (OCT compound, Tissue-Tek, #14291) were cut to 4-5µm with a cryostat (Leica CM 1950, Leica Biosystems) and positioned onto SuperFrost plus slides (R.Langenbrinck ...
-
bioRxiv - Biochemistry 2024Quote: ... The head of the mouse was positioned under the tip (5mm diameter) of the impactor (Leica Impact One Electromagnetic Impactor). The mouse was held over a sheet of filter floss to allow free linear and rotational movement of the head and neck ...
-
bioRxiv - Neuroscience 2024Quote: ... Hippocampal-containing sections were cut into 10 µm thick mouse brain coronal slices using a CM3050 S cryostat (Leica, Germany) at −20°C ...
-
bioRxiv - Immunology 2024Quote: ... ýIII-Tubulin (1:250) and human HLA-DR (1:250, LN-3, Leica/Novocastra) followed by staining with a cocktail of DAPI and fluorochrome-conjugated secondary antibodies and mounting on histological slides.
-
bioRxiv - Cell Biology 2020Quote: ... CD56 (1:200, Leica Biosystems), or CD163 (1:200 ...
-
bioRxiv - Developmental Biology 2019Quote: ... p63 (1:50, Leica Biosystems), CK8/18 (1:50 ...
-
bioRxiv - Developmental Biology 2019Quote: ... p63 (1:50, Leica Biosystems), CK8/18 (1:50 ...
-
bioRxiv - Neuroscience 2021Quote: Acute mouse coronal slices containing hippocampus region (250 μm) were prepared from anesthetic mice using a vibrating tissue slicer (Leica VT1000S) in ice-cold artificial cerebrospinal fluid (ACSF ...
-
bioRxiv - Genomics 2021Quote: ... primary antibody was diluted 1:10 in the peptide solution directly.) The primary antibodies were detected by 30 minute incubation with HRP-labeled anti-mouse secondary antibody (catalog# PV6114, Leica Microsystems) followed by detection with 3,3′-Diaminobenzidine (catalog# D4293 ...
-
bioRxiv - Evolutionary Biology 2021Quote: Embryonic bat heads and embryonic E16.5 mouse heads were submerged in chilled lx PBS and imaged using a stereo microscope with brightfield illumination (Leica M165 FC) in standard anatomical views ...
-
bioRxiv - Neuroscience 2021Quote: ... and −1.46 mm (dorsal hippocampus) from bregma according to the Paxinos and Franklin mouse brain atlas54 in triplicate using a cryostat (Leica, Germany) and dry mounted on coated Superfrost Plus slides (Thermo Fischer Scientific ...
-
bioRxiv - Neuroscience 2021Quote: ... mouse brain hemispheres prefixed with 4% PFA for 48 hours were sectioned at 50 μm using a Vibratome (Leica Microsystems Inc). Immunostaining of free-floating sections was performed using the ABC kit (Vector Laboratories ...
-
bioRxiv - Neuroscience 2021Quote: Embryonic left and right hippocampus was microdissected from genotyped P21 mouse brains using a laser-assisted capture microscope (Leica ASLMD instrument) with Leica polyethylene naphthalate membrane slides as described in Lepagnol-Bestel et al ...
-
bioRxiv - Neuroscience 2022Quote: ... The mouse brains were dehydrated in 30% sucrose and sectioned at a thickness of 40 μm with a cryostat (Leica Microsystem), and the brain sections were immunostained for mCherry (1:1000).
-
bioRxiv - Neuroscience 2021Quote: Mouse brains were fixed in 4% paraformaldehyde and cut into 40-μm-thick sections using a vibratome (Leica Biosystems, Nussloch, Germany). The sections were blocked using blocking solution (X0909 ...
-
bioRxiv - Immunology 2020Quote: ... Non-invasive intravital imaging of mouse ear was performed using Leica DIVE (Deep In Vivo Explorer) inverted confocal microscope (Leica Microsystems) with full range of visible lasers (Spectra Physics) ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... using microhematocrit capillary tubes (10 μL) and measured the hematocrit rate for each mouse using a Critocaps Micro-Hematocrit Capillary Tube Reader (Leica Microsystems) (22).
-
bioRxiv - Neuroscience 2022Quote: ... then the right OB from each mouse was embedded in 6 % agarose and sliced coronally at 50 μm using a vibratome (Leica VT1000S).
-
bioRxiv - Biophysics 2023Quote: 5 µm sections of formalin-fixed and paraffin-embedded (FFPE) mouse tail specimens were H&E-stained by using an automated stainer (Leica XL) at University of Manchester’s Histology Core as previously described [11 ...
-
bioRxiv - Plant Biology 2023Quote: ... 100% ethanol) and incubated overnight in a 1:1 solution of 100% ethanol:Historesin solution (Leica). The solution was exchanged with Historesin and the samples incubated again overnight at 4°C ...
-
bioRxiv - Systems Biology 2023Quote: ... in a sequential order as shown: 1) Opal 480/CD71 (1:80, 10F11, Leica concentrate), 2 ...
-
bioRxiv - Molecular Biology 2019Quote: ... Images were acquired using Leica DFC 3000G (Bin 1×1, Gamma1) camera and processed by Leica LAS X imaging software.
-
bioRxiv - Neuroscience 2022Quote: In vivo images of mouse retinal structure were obtained with spectral domain-optical coherence tomography (SD-OCT) (Leica Microsystems, Buffalo Grove, IL). The mice received 1% tropicamide drops to stimulate eye dilation and were anesthetized with ketamine and xylazine (50 and 5 mg/kg body weight ...
-
bioRxiv - Neuroscience 2022Quote: ... each mouse brain was sectioned into 30-μm-thick coronal slices with a freezing microtome (CM30503, Leica Biosystems, Buffalo Grove, IL, USA). The sections were first washed in PBS three times for 5 min each and then incubated in blocking solution containing 10% normal donkey serum (017-000-121 ...
-
bioRxiv - Neuroscience 2021Quote: ... and coronal sections of the mouse brain containing the dentate gyrus and prefrontal cortex were then cut using a VT1200S microslicer (300 μm) (Leica, Wetzlar, Germany). Micro-dissected areas were post-fixed at 4C for 2 hours in 2% osmium tetroxide in 0.1mol/l Cacodylate Buffer ...
-
bioRxiv - Cancer Biology 2021Quote: ... paraffin-embedded tissues was carried out on a Discovery XT automated slide stainer using a mouse anti-human monoclonal antibody for FOLR1 (Novocastra Clone BN3.2; Leica Biosystems, Wetzlar, Germany) at 15 µg/ml after antigen retrieval with Cell Conditioning 1 (CC1 ...
-
bioRxiv - Neuroscience 2022Quote: ... each mouse brain was sectioned into 30-μm-thick coronal slices with a freezing microtome (CM30503, Leica Biosystems, Buffalo Grove, IL, USA). The sections were first washed in PBS three times for 5 min each and incubated in blocking solution for 2.5h at room temperature ...
-
bioRxiv - Neuroscience 2019Quote: ... The left and right hippocampus was micro dissected from genotyped P21 mouse brains using a laser-assisted capture microscope (Leica ASLMD instrument) with Leica polyethylene naphthalate membrane slides as described in ...