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Citations for Leica :
151 - 189 of 189 citations for Boc 6 fluoro DL tryptophan since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... staining process involved serial repetitions of the following for each biomarker: epitope retrieval/stripping with ER1 (citrate buffer pH 6, AR996, Leica Biosystems) or ER2 (Tris-EDTA buffer pH9 ...
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bioRxiv - Cell Biology 2023Quote: ... Dried samples were rotary-shadowed with 2 nm of platinum and 6 nm of carbon using a high vacuum sputter coater (Leica Microsystems). The resultant platinum-replica was floated off the glass with hydrofluoric acid (5%) ...
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β-1,6-glucan plays a central role in the structure and remodeling of the bilaminate fungal cell wallbioRxiv - Microbiology 2024Quote: ... covered by 6 mm type B carrier (side flat) and frozen with a high-pressure freezing machine (EM ICE, Leica microsystems) as previously described112 ...
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bioRxiv - Neuroscience 2024Quote: ... were taken from 6 mice/group/sex using a DFC9000 GT VSC-12292 monochrome camera with Las X Navigator software (Leica Microsystems) at x20 magnification ...
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bioRxiv - Neuroscience 2024Quote: FFPE blocks of post-mortem human midbrains were sectioned at 6 µm thickness using a rotary microtome (HistoCore MULTICUT R Rotary, Leica Biosystems). The sections were mounted on Fisher Scientific slides (catalog #12-550-15 ...
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bioRxiv - Biochemistry 2024Quote: ... Dried samples were rotary-shadowed with 2 nm of platinum and 6 nm of carbon using a high vacuum sputter coater (Leica Microsystems). The resultant platinum replica was floated off the glass with hydrofluoric acid (5%) ...
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bioRxiv - Cancer Biology 2024Quote: ... Wound closure was measured at regular intervals (0-hour, 3-hour, 6-hour, 9-hour) using an inverted microscope (Leica DMi1). The wound closure distances at different time points were normalized to the distance at 0hour to calculate the migration rate.
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bioRxiv - Molecular Biology 2022Quote: ... Green siliques were harvested 12 d after flowering from 4-6 week old plants and opened using fine needles under a dissecting microscope (Leica MZ9.5, Germany). Images of opened siliques were captured using a Leica DFC290 (Germany ...
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bioRxiv - Neuroscience 2022Quote: ... embedded in 6% low melting agarose and cut into slices of 100 μm with a vibratome Leica VT 1000S (Leica, Bensheim, Germany). The slices were washed twice in 1x PBS ...
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bioRxiv - Neuroscience 2020Quote: ... containing dorsal hippocampus (from 3, 6, 9, 12, 15, 19, and 24 month-old) were cut from PFA-fixed hemispheres (Leica vibratome VT1000P) and stored in 0.1M phosphate-buffered saline (PBS ...
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bioRxiv - Cell Biology 2022Quote: ... The resin blocks were cut longitudinally into 6 μm slices of the femur distal metaphysis using a Leica SP1600 microtome (Leica Microsystems, Germany). Fluorescence signals of calcein and AR from the ROIs were captured using a fluorescence microscope (Q500MC ...
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bioRxiv - Molecular Biology 2020Quote: ... the number of colonies exhibiting correct fluorescence were counted (EGFP+ for 2-insert assembly, EGFP+/mCherry+ for 6-insert assembly) using a fluorescence stereoscope (Leica M165 FC). Accuracy was calculated as the percentage of colonies exhibiting correct fluorescence ...
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bioRxiv - Bioengineering 2022Quote: ... The distribution of cells in the microgel matrix was assessed after 6 h using a Leica SP8 DIVE multiphoton microscope (Leica Microsystems, Germany) with 16x water immersion lens ...
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bioRxiv - Cell Biology 2022Quote: ... Dried samples were then rotary shadowed with 2 nm of platinum and 4-6 nm of carbon using the ACE600 (Leica Microsystems, Germany). Areas of interest from the resulting platinum replicas were separated from glass with 5% hydrofluoric acid and deposited on carbon-coated EM grids (200 mesh formvar/carbon ...
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bioRxiv - Cell Biology 2022Quote: ... Dried samples were then rotary shadowed with 2 nm of platinum (sputtering) and 4-6 nm of carbon (carbon thread evaporation) using an ACE600 metal coater (Leica Microsystems, Germany). The resultant platinum replica was floated off the glass with hydrofluoric acid (5%) ...
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bioRxiv - Plant Biology 2022Quote: ... Nodule primordia and nodules were embedded in 6% low-melting agarose and sliced into 40 - 50 µm thick sections using a vibrating-blade microtome (Leica VT1000 S).
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bioRxiv - Neuroscience 2022Quote: ... the fresh retinal wholemount was embedded in 6% agar and cut into 250-μm slices with a vibrating blade microtome (Leica VT100 S). For nocodazole treatment ...
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bioRxiv - Microbiology 2024Quote: ... containing 4‘,6-diamidino-2-phenylindole (DAPI) and imaged as midsections by confocal laser scanning microscopy (Leica SP8: Leica Microsystems, Wetzlar, Germany). To prevent cross talk between the channels for the different fluorochromes ...
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Phospholipase C β4 promotes RANKL-dependent osteoclastogenesis by interacting with MKK3 and p38 MAPKbioRxiv - Cell Biology 2024Quote: ... The resin blocks were cut longitudinally into 6-μm slices of the tibia using a Leica RM2165 rotary microtome equipped with a tungsten blade (Leica Microsystems, Germany). Fluorescence signals emitted by calcein green and alizarin red were recorded using a fluorescence microscope (Leica Microsystems) ...
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bioRxiv - Cell Biology 2023Quote: ... Dried samples were then rotary shadowed with 2 nm of platinum (sputtering) and 4-6 nm of carbon (carbon thread evaporation) using an ACE600 metal coater (Leica Microsystems, Germany). The resultant platinum replica was floated off the glass with hydrofluoric acid (5%) ...
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bioRxiv - Cell Biology 2022Quote: ... the 6-well plate containing the cells were mounted onto a confocal microscope (Leica TCS SP8 with a 10Å∼0.4 NA objective). They were then maintained at 37ºC (stage heater ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were mounted on stubs using colloidal silver as an adhesive and sputter coated with 6 nm gold (Leica Coater ACE 200). Cells were imaged using FEI Quanta 3D scanning electron microscope operated at an accelerating voltage of 2 kV ...
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bioRxiv - Neuroscience 2023Quote: ... Images of cultured ependymocytes co-treated with Sir-Actin and calcein AM were captured at 10-s intervals for 12 minutes while that of co-treated with Sir-Actin and fluo-4 AM were captured at 10-s intervals for 6 minutes using confocal scanning microscope (DMi8, Leica, Wetzlar, Germany). The intensity of F-actin area from all Sir-Actin positive cells in a field of recording were included in the statistics ...
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bioRxiv - Neuroscience 2023Quote: ... The fluorescence signals of Fluo-4 were monitored at 10-s intervals for 6 minutes by confocal scanning microscope (DMi8, Leica, Wetzlar, Germany). As previously described 46 ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: Perfused brains (n = 20 / sex; 40 total) were sliced coronally into 6 series at 30 µm on a vibrating microtome (Leica VT 1000S). The samples were stored at -20 °C in cryoprotectant until immunohistochemical (IHC ...
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bioRxiv - Neuroscience 2021Quote: ... then sectioned coronally into 20-μm-thick slices (1:6 series of the PVN) using a Leica CM1900 cryostat (Leica Microsystems, Wetzlar, Germany). Immunofluorescence staining was performed on brain sections derived from P10 male tdTomato-Crh (Crh-IRES-Cre;Ai14 ...
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bioRxiv - Neuroscience 2021Quote: ... isogenic samples reared in the same environment were highpressure frozen (Leica HPM100 for datasets 1-5 and Leica ICE for datasets 6-8) at different stages of post-embryonic development ...
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bioRxiv - Plant Biology 2020Quote: ... Dried samples were sputter-coated with argon–platinum plasma at a distance of 6–7 cm and 45 mA intensity for 15 sec in a sputtering chamber (Leica Microsystems EM MED020). Scanning electron micrographs were acquired using an AURIGA compact FIB-SEM (Zeiss ...
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bioRxiv - Pathology 2022Quote: ... Pictures of 4-6 different fields per sample were taken under a Leica DM5000 B microscope equipped with a digital CCD (Leica Camera, Wetzlar, Germany).
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bioRxiv - Cell Biology 2021Quote: ... resin blocks were mounted onto SEM pins with silver epoxy and sputter coated with 6 nm of iridium (Leica ACE 600, Vienna, Austria) at a 45° angle with rotation on a planetary stage to ensure the entire block was coated.
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bioRxiv - Cancer Biology 2024Quote: ... Antigen retrieval was performed in BOND Epitope Retrieval Solution 1 (pH 6) or 2 (pH 9) (ER1, ER2, Leica Biosystems, Cat. AR9961, AR9640) at 95°C ...
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bioRxiv - Neuroscience 2023Quote: ... Brain regions containing the possible contact sites were re-embedded into resin blocks and 70 nm thick sections were cut with an ultra-microtome (Leica EM UC 6). Electron microscopic images were taken either with a Hitachi H-7100 transmission electron microscope or a FEI series electron microscope to reveal synaptic contacts between BFPVNs and target cell types in the MS ...
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bioRxiv - Neuroscience 2024Quote: ... Brains were sectioned at 50 µm or 6 μm on a Leica CM3050 S cryostat set to −20°C for both the object and chamber temperature (Leica Biosystems, Nussloch, Germany). We collected sections from the primary somatosensory cortex (coronal sections from bregma ...
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bioRxiv - Cell Biology 2021Quote: ... stained with DAPI (4’,6-diamidino-2-phenylindole) for visualisation of the nuclei and analysed by confocal microscopy using an inverted microscope (Leica DMI6000, objective lens 100×).
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bioRxiv - Cell Biology 2021Quote: ... was deposited on an EM copper grid (400-mesh, Plano G2400C, coated with 6 nm thick carbon produced with a Leica EM ACE600 sputter coater), incubated for 30-60 sec and manually blotted with filter paper ...
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bioRxiv - Microbiology 2021Quote: ... coverslips were mounted on aluminum stubs with carbon adhesive tabs and coated with 10 nm of carbon and 6 nm of iridium (Leica ACE 600, Vienna, Austria). SEM images were acquired on a FE-SEM (Zeiss Merlin ...
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bioRxiv - Microbiology 2022Quote: ... Samples were then mounted on aluminum stubs with carbon adhesive tabs and coated with 5 nm of carbon and 6 nm of iridium (Leica ACE 600, Vienna, Austria). SEM images were acquired on an FE-SEM (Zeiss Merlin ...
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bioRxiv - Cell Biology 2022Quote: ... or for 4–6 s at 4°C and ~ 86% and plunge frozen using a Leica EM GP (Leica Microsystems Inc, Buffalo Grove, IL). The samples were imaged in an FEI Titan Krios electron microscope operated at 300 kV with a K2 Summit camera and energy filter (Gatan ...
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bioRxiv - Immunology 2023Quote: ... coverslips were mounted on aluminum stubs with carbon adhesive tabs and coated with 10 nm of carbon and 6 nm of iridium (Leica ACE 600, Vienna, Austria). SEM images were acquired on a FE-SEM (Zeiss Merlin ...