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Citations for Leica :
151 - 200 of 208 citations for Adenylyltransferase And Sulfurtransferase MOCS3 MOCS3 Antibody Biotin since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... CD34 antibody was applied at ambient temperature for 30 min (1:50, cat# NCL-L-END, Leica, Bannockburn, IL) followed by an anti-mouse secondary antibody conjugated to HRP and visualized with Opal 690 (cat#OP-001006 ...
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bioRxiv - Cancer Biology 2021Quote: ... and the following primary and secondary antibodies were used: rabbit anti-p53 (Leica Biosystems, NCL-L-p53-CM5p, 1:5000), rabbit anti-Mettl3 (Abclonal ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were harvested to prepare chromatin for immunoprecipitation using either p53 polyclonal antibodies (Leica Biosystems, Cat # NCL-L-p53-CM5p) or Mettl3 polyclonal antibodies (Abclonal ...
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bioRxiv - Developmental Biology 2021Quote: ... The secondary antibodies were washed and the brains were mounted in Slowfade and imaged at a confocal microscope (Leica SP8) using a 63x glycerol objective ...
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bioRxiv - Cancer Biology 2020Quote: ... 3 μm sections from the TMA-2 human adenocarcinoma cohort were duplex stained using a Ventana autostainer with antibodies for cytokeratin AE1/AE3 (1:250, #NCL-L-AE1/AE3, Leica detected with Roche discovery yellow chromogen ...
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bioRxiv - Cell Biology 2021Quote: ... Primary antibodies were incubated for 60 minutes and detected using the BOND™ Polymer Refine Detection System (DS9800, Leica Biosystems) as per manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... V5-tag-specific antibody was used at 1:100 dilution (Cat # R960-25, Invitrogen) (. Cells were imaged using a Leica DMi8 confocal microscope (63x ...
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bioRxiv - Cancer Biology 2021Quote: ... The slides were incubated with a secondary antibody (rabbit anti-mouse IgG, BOND Polymer Refine Detection System, Leica, Germany, DS9800) for 8 minutes at room temperature ...
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bioRxiv - Genetics 2021Quote: Assessment of Aβ deposition was carried out by immunohistochemical staining of paraffin-embedded sections (3–5 μm-thick) with a mouse monoclonal (S6 F/3D) anti-Aβ antibody (Leica Biosystems Newcastle Ltd ...
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bioRxiv - Cancer Biology 2022Quote: ... Primary antibodies were incubated for 30 minutes and detected using the BOND™ Polymer Refine Detection System (DS9800, Leica Biosystems) as per manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: ... Bio SB) or anti-human CD163 (clone EP324, Bio SB) followed by an HRP-conjugated anti-mouse secondary antibody (Leica) and revealed with the peroxidase substrate 3 ...
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bioRxiv - Cancer Biology 2023Quote: ... The following antibodies were used at the indicated dilution and indicated antigen retrieval method: Ki67 (CST 12202, 1:1000, ER2 Leica), CD8a (eBioscience 14-0808-82 ...
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bioRxiv - Genomics 2023Quote: ... slides were deparaffinized and automatically stained with specific antibodies against synovial cell markers (PECAM-1, DAKO JC/70a, 1:10; CD20, Ventana Roche L26, prediluted; CD3, Leica LN10 ...
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bioRxiv - Cancer Biology 2022Quote: ... IHC staining for Ki67 and MGP antibodies was performed using Bond III IHC autostainer for full Automated Immunohistochemistry (Leica biosystems). Antigen was unmasked with Tris-EDTA pH 9 (Bond Epitop Retrival Solution 2 Leica ...
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bioRxiv - Immunology 2024Quote: ... The following primary antibodies were used at the consequence and with indicated dilutions and CF dyes: CD20 (Leica Biosystems PA0200), 1:1 with CF488 ...
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bioRxiv - Cancer Biology 2024Quote: ... The cells were then washed three times with 1X PBS and incubated with geminin-L-CE primary antibody (Leica Biosystems) for 1 hour ...
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bioRxiv - Genomics 2024Quote: ... Clinical determination of hormone receptor status and HER2-amplification status in the 79-patient validation cohort was performed at the Clinical Laboratory Improvement Amendments (CLIA)-certified MGH Clinical Immunohistochemistry and MGH Center for Integrated Diagnostics laboratories following standard protocols using monoclonal antibody clone 6F11 for ER and clone 16 for PR (Leica Microsystems ...
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bioRxiv - Cancer Biology 2024Quote: ... The following antibody was used: anti CD31 (CST, 3528, clone 89C2, 1:1600, antigen retrieval Leica ER1 AR9961 for 30min).
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bioRxiv - Cell Biology 2020Quote: ... The antibodies were washed in TBS three times for three minutes at a time before the Novolink post primary block (Leica biosystems) was applied for 30 minutes at room temperature ...
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bioRxiv - Immunology 2021Quote: ... Nucleocapsid Antibody (SINO BIO, 40143-R019) through LEICA BOND RX 1h RT and developed with Bond Polymer Refine Detection (Leica, DS9800). Brightfield images were acquired through an Aperio Scanscope System CS2 microscope and an ImageScope program (Leica Biosystem ...
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bioRxiv - Immunology 2021Quote: Primary (1ry) Antibodies were incubated for one hour and detected using the BOND™ Polymer Refine Detection System (DS9800, Leica Biosystems) as per manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Six consecutive staining cycles were performed using the following 1ry Antibody-Opal fluorophore pairings: CD4 (clone 4B12, NCL-L-CD4-368 (Leica Novocastra) – Opal 520) ...
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bioRxiv - Cell Biology 2021Quote: ... Rabbit monoclonal ACE2 (1:400) and anti-αvβ6 integrin (1:3000) was diluted in Leica antibody diluent (RE AR9352, Leica, Biosystems, UK) and incubated with the sections overnight at 4°C ...
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bioRxiv - Pathology 2022Quote: ... slides were stained and processed using the 17B4 primary antibody on a Leica BOND-III autostainer (Leica Biosystems, Buffalo Grove, IL).
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bioRxiv - Cancer Biology 2020Quote: ... Visualization after staining with the antibodies was performed using a high-resolution slide scanner (Leica SCN400FL, Leica microsystems, GmbH, Wetzlar, Germany) at 20 × magnification ...
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bioRxiv - Physiology 2020Quote: ... after which they were incubated with antibodies against GPT (AP7468b, Abgent; 1:200) and GPT2 (PAD854Mu01, Cloud-Clone Inc.; 1:200) antigens in BONDTM primary antibody diluent (AR9352, Leica Biosystems). For skeletal muscle fiber size visualization ...
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bioRxiv - Immunology 2021Quote: ... Nucleocapsid Antibody (SINO BIO, 40143-R019) through LEICA BOND RX 1h RT and developed with Bond Polymer Refine Detection (Leica, DS9800). Brightfield images were acquired through an Aperio Scanscope System CS2 microscope and an ImageScope program (Leica Biosystem ...
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bioRxiv - Cancer Biology 2020Quote: ... The standard protocol was followed apart from the primary antibody incubation which was extended to 1 hr at room temperature and the post primary step was excluded.27 BMI1 antibody was used at a 1:200 dilution and antigen retrieval was performed with E1 (Leica Biosystems) retrieval solution for 20 min ...
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bioRxiv - Genomics 2022Quote: Five μm sections of formalin-fixed paraffin-embedded tissues from postmenopausal women were stained with hematoxylin and eosin or with commercially available antibodies using the Leica Bond RX automated stainer (Leica Biosystems) and reviewed by gynecologic pathologists ...
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bioRxiv - Cancer Biology 2023Quote: ... Goat anti-rabbit-HRP was used as the secondary antibody with the BOND polymer refine detection system (Leica, catalog no. DS9800). Images were taken by Zeiss LSM710 confocal microscope with a 10X ...
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bioRxiv - Cell Biology 2023Quote: ... and immunostained using primary and secondary antibodies (listed in ESM Methods) and imaged using a TCS SP5 confocal microscope (Leica, Germany). Immunostaining of paraffin-embedded pancreas from mice with beta cell-selective deletion of Dnmt3a was performed according to [10 ...
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bioRxiv - Immunology 2024Quote: ... incubated with anti-CD20 (CM-0221, Jiehao bio, China) for 60 minutes and then treated with peroxidase-conjugated (HRP) secondary antibody (DS9800, Leica, Germany) for 10 minutes ...
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bioRxiv - Cancer Biology 2024Quote: We accessed a TNBC data from a previous study in which mouse anti-human monoclonal AR primary antibody (AR-318-L-CE, Leica Biosystems) was used to detect AR in a tissue microarray (TMA ...
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bioRxiv - Cell Biology 2024Quote: ... the sections were incubated with the following primary antibodies: mouse monoclonal CD61 (ready to use, clone 2F2 cod. PA0308, Leica Biosystems) and rabbit polyclonal MPO (myeloperoxidase ...
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bioRxiv - Immunology 2021Quote: ... primary antibody incubation at 1/400 dilution for 30 min then detection using the BOND™ Polymer Refine Detection System (DS9800, Leica Biosystems) as per manufacturer’s instructions.
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bioRxiv - Cancer Biology 2021Quote: ... paraffin-embedded tissues was carried out on a Discovery XT automated slide stainer using a mouse anti-human monoclonal antibody for FOLR1 (Novocastra Clone BN3.2; Leica Biosystems, Wetzlar, Germany) at 15 µg/ml after antigen retrieval with Cell Conditioning 1 (CC1 ...
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bioRxiv - Cancer Biology 2022Quote: ... and incubated overnight at 4°C in 0.05% Digitonin-Antibody buffer containing a rabbit anti mouse-p53 Ab (1:100 Leica, NCL-p53-CM5p). Samples incubated with rabbit IgG (1:150 ...
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bioRxiv - Cancer Biology 2022Quote: ... was applied on tissue sections for 25 minutes incubation and the antigen-antibody binding was detected with Bond polymer refine detection (Leica Biosystems, DS9800). The tissue sections were covered with cover glasses ...
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bioRxiv - Physiology 2022Quote: ... Slides were washed and incubated with anti-rabbit poly-HRP-IgG secondary antibody (Novolink Polymer Detection System, Leica Biosystems, cat# RE7200-CE) for 30 minutes at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: The assay consisted of the following antibodies and corresponding dilution factor: CK AE1/AE3 (Leica Biosystems, ref. AE1/AE3-601-L-CE) was applied at 1:250 for 28 minutes at 37°C ...
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bioRxiv - Neuroscience 2020Quote: ... Slices were prepared at a thickness of 70 μm for imaging intrinsic fluorescence or 40 µm for antibody staining (Leica VT 1000S vibratome). For immunohistochemistry ...
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bioRxiv - Pathology 2021Quote: ... Slides were then stained with anti-Iba1 antibody (1:2000; Wako; 019- 19741 Richmond, VA) using the Bond Polymer Refine Red Kit (cat. DS9390 Leica Biosystem, Richmond, IL). The slides were stained using a pan-cytokeratin antibody (1:1000 ...
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bioRxiv - Neuroscience 2020Quote: ... anti-glial fibrillary acidic protein (GFAP; mouse anti-human monoclonal antibody GA5, with bond heat-induced epitope retrieval, epitope retrieval time 10 min, PA0026; Leica Biosystems, Wetzlar, Germany); anti-ionized calcium-binding adapter molecule 1 (IBA1 ...
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bioRxiv - Neuroscience 2023Quote: ... Colocalization of the antibody immunofluorescence signal and endogenous fluorescence of NG2 glia was examined using the Leica Stellaris confocal platform (Leica Microsystems, Wetzlar, Germany). The immunopositive areas of the cortex surrounding the ischemic injury were further quantified using Fiji ImageJ software (NIH ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Glial fibrillary acid protein (GFAP) Iimmunohistochemistry was carried out using GFAP primary monoclonal antibody and the Novocastra™ and Novolink DM polymer detection system (Leica Biosystems, UK) as described previously (39–42).
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bioRxiv - Neuroscience 2023Quote: Neuron specific enolase (NSE) immunohistochemistry was carried out using NSE primary monoclonal antibody and the Novocastra™ and Novolink DM polymer detection system (Leica Biosystems, UK) as described previously [54 ...
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bioRxiv - Immunology 2023Quote: ... Formalin-fixed paraffin-embedded (FFPE) sections of 4 μm thickness were stained with antibodies specific for CD21 (predilute, clone 2G9, Leica Biosystems, Danvers, MA), CD3 (predilute ...
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bioRxiv - Cancer Biology 2023Quote: ... rehydrated and subsequently stained with hematoxylin and eosin (H&E) and appropriate antibodies (Supplementary Table 3) using the automated system BOND RX (Leica Biosystems, Newcastle, UK). Visualization was by the Bond Polymer Refine Detection kit (Leica Biosystems ...
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bioRxiv - Cancer Biology 2024Quote: ... Four-µm-thick FFPE sections were incubated with the antibodies listed in Supplementary Table S1 on a BondTM Automated System (Leica Microsystems, Wetzlar, Germany), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... in TBST buffer for IF and subjected to staining with the following antibodies overnight at 4C: p53 (Leica, P53-CM5P-L 1:100), GFP/YFP (Abcam ...