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Citations for Leica :
151 - 200 of 869 citations for 4 Hydroxy 7 trifluoromethyl quinoline 3 carbohydrazide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... and MgCl2, 7 (pH 7.4, 320 mOsm) and 400μm coronal sections were cut using VT1200S vibratome (Leica Biosystems, Germany). The sections were allowed to recover for at least 30 minutes in recording aCSF (in mM) ...
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bioRxiv - Neuroscience 2024Quote: ... Olfactory bulbs were dissected and post-fixed in 1% PFA for 2-7 days and embedded in 5% agarose and sectioned into 50µm slices using a vibratome (VT1000S, Leica). Free-floating slices were washed with PBS and incubated in 5% normal goat serum in PBS/Triton/azide (0.25% Triton ...
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bioRxiv - Pathology 2024Quote: ... Images were obtained using Zeiss Axioscope 7 (Oberkochen, Germany) or Leica TCS SP8 Confocal Microscopy (Leica Microsystems, Wetzlar, Germany). CD45+ cells were quantified on 63X images from randomly selected 17 images (each genotype ...
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bioRxiv - Plant Biology 2020Quote: ... Sections (3-4μm) cut by rotary microtome (Leica RM2265) were stained with 0.01 % aqueous toluidine blue ...
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bioRxiv - Neuroscience 2021Quote: ... and 3% lead citrate (Leica Ultrostain II, Leica Microsystems). Images were collected semiautomatically using custom software guiding an FEI Magellan XHR 400L54.
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bioRxiv - Neuroscience 2021Quote: ... and 3% lead citrate (Leica Ultrostain II, Leica Microsystems). Images were collected semiautomatically using custom software guiding an FEI Magellan XHR 400L54.
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bioRxiv - Bioengineering 2023Quote: ... and 20 min in 3% lead citrate (Leica, Germany). TEM images were acquired with a 200 kV TEM JEM 2100Plus (Jeol ...
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bioRxiv - Molecular Biology 2024Quote: ... 3 µm sections were cut on a microtome (Leica), rehydrated through an alcohol series according to standard laboratory procedures and then stained with a PAS staining kit (TCS Biosciences ...
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bioRxiv - Cancer Biology 2023Quote: ... 3 µm sections were obtained with a microtome (Leica RM2255 Fully Automated Rotary Microtome) ...
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bioRxiv - Cell Biology 2024Quote: ... and Ultrostain-2 3% Lead citrate (Leica Microsystems#16707235). Images acquisition was done with JEOL JEM-1230 transmission electron microscope ...
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bioRxiv - Microbiology 2024Quote: ... and IBA1 using the Bond- RX/3 autostainer (Leica) with the BPRD kit (DS9800 ...
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bioRxiv - Neuroscience 2021Quote: ... post-fixed in 4% PFA overnight at 4°C and afterwards cut with a vibratome (Leica Microsystems, Wetzlar Germany) in 100 μM thick sequential sagittal slices ...
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bioRxiv - Neuroscience 2023Quote: ... Brains were postfixed overnight in 4% PFA at 4°C before 50μm coronal brain sections were cut using a vibratome (VT1200S, Leica). Slices were blocked (TBS with 0.5% Triton X-100 and 10% donkey serum ...
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bioRxiv - Neuroscience 2023Quote: ... post-fixed in 4% PFA overnight at 4 °C and 100um thick coronal vibratome sections were made (Leica VT1000S). Images of both ipsilateral (injected ...
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bioRxiv - Neuroscience 2024Quote: ... Brains were extracted and post-fixed overnight at 4°C in 4% PFA (pH = 7.2) and 40-μm coronal sections were collected using a vibratome (VT1000S, Leica). After a 15-min incubation in a 4,6-Diamidino-2-phenylindole dihydrochloride hydrate (DAPI ...
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bioRxiv - Neuroscience 2022Quote: ... 1-hour each), xylene (3×, 1.5-hour each), paraffin (3 ×, 2-hours each) (Fisher, UK) in tissue processor (Leica TP1020 tissue processor, UK) and embedded in paraffin using embedding station (Leica HistoCore Arcadia H embedding station ...
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bioRxiv - Physiology 2022Quote: Aortic roots at optimal cutting temperature were embedded in NEG-50 and cut into 7 μm thick sections using a cryostat (CM1510 S; Leica). These sections were stained with Oil Red O/hematoxylin to measure lipid depot using the protocol below ...
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bioRxiv - Neuroscience 2023Quote: ... adult brains were fixed in 4% PFA and included in 7% low-melting agarose in PBS 1X and sectioned at 100 µm using a vibratome (Leica). Sections were mounted with Glycergel (Dako ...
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bioRxiv - Neuroscience 2024Quote: ... The samples were then embedded in 7% agarose and cut into 0.3 mm sagittal sections using a vibratome (Leica VT1000s). The slices were incubated overnight at 37° C in PBS ...
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bioRxiv - Genomics 2023Quote: ... Sections were cut to a thickness of 7 μm with a Leica HistoCore AUTOCUT Rotary Microtome (Leica Biosystems, Wetzlar, Germany) and Leica RM2245 ...
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bioRxiv - Bioengineering 2023Quote: ... Images were taken with either a fluorescent light microscope (Axio Observer 7, Zeiss, Oberkochen, Germany) (Figure 3B and 5A and B) or a confocal microscope (TCS SP5X, Leica Microsystems CMS GmbH ...
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bioRxiv - Developmental Biology 2024Quote: ... and sectioned to a thickness of 7 µm for the testis and 5 µm for the epididymis using an Ultrathin Microtome from Leica, Germany ...
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bioRxiv - Pathology 2024Quote: ... Images were acquired with Zeiss Axioscope 7 microscope (Oberkochen, Germany) or Leica TCS SP8 Confocal Microscopy (Leica Microsystems, Wetzlar, Germany).
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bioRxiv - Neuroscience 2020Quote: ... All mouse brain tissue specimens were fixed by immersion in 4% PFA overnight at 4 °C and sectioned at 50 μm using a vibratome (Leica).
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bioRxiv - Neuroscience 2022Quote: ... Brains were post-fixed overnight in 4% PFA at 4 °C and sectioned at 40 μm using a vibratome (VT1000S, Leica) except for experiments involving the RNAscope kit (ACD Bio.) ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... Brains were removed and post-fixed in 4 % PFA in PBS at 4 °C for 24 h and then sectioned using a vibratome (Leica) with thicknesses of 40 μm ...
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bioRxiv - Neuroscience 2024Quote: ... then fixed overnight at 4 °C in 4% PFA and coronally sectioned at 35 μm intervals using a freezing microtome (CM3050S, Leica). Brain sections were permeabilized with a solution containing 5% bovine serum albumin and 1% Triton X-100 in PBS for 40 minutes at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... The brain was then extracted and saved in 4% PFA overnight at 4 °C before being resected into 70 μm slices using a VT1000s vibratome (Leica Biosystems ...
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bioRxiv - Neuroscience 2022Quote: ... Brains were then post-fixed for overnight in 4% paraformaldehyde at 4 and sectioned into 40 µm sagittal slices using a vibratome (VT1000S, Leica). Brain sections were incubated in blocking solution (0.2% Triton X-100 in PBS with 5% normal goat serum ...
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bioRxiv - Microbiology 2022Quote: ... Then tissues were sectioned to 3 μm by microtome (Leica). Next ...
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bioRxiv - Immunology 2021Quote: ... 3 x H2O) on a linear stainer (Leica Biosystems, ST4020). Epitope retrieval was performed at 97C for 10 min with the Dako Target Retrieval Solution pH 9 (Agilent ...
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bioRxiv - Biophysics 2022Quote: ... The 3D model in Figure 3 was analyzed by Leica LAS X ...
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bioRxiv - Neuroscience 2021Quote: ... post-fixed in 4% PFA for 48 h at 4 °C and cut into 60 μm coronal sections using a vibratome (VT1200S, Leica, Germany). The sections were post-fixed (1 h) ...
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bioRxiv - Neuroscience 2023Quote: ... incubated in 4% paraformaldehyde overnight at 4°C, and sectioned coronally (thickness, 50–100 μm) using a vibratome (VT1200S, Leica Microsystems). Brain slices were incubated with 0.1% Triton X-100/phosphate-buffered saline for 10Cmin and blocked with Blocking-One P for 30 min ...
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bioRxiv - Neuroscience 2024Quote: ... post-fixed in 4% PFA for 48 h at 4°C and cut into 100 μm coronal sections using a vibratome (VT1200S, Leica, Germany). The sections were post-fixed (1 h) ...
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bioRxiv - Neuroscience 2024Quote: ... Extracted brains were then stored in 4% PFA overnight at 4 before being sliced at 60 µm using a vibratome (Leica VT1000S). PVN and rPa regions were identified via the Allen Brain Atlas ...
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bioRxiv - Neuroscience 2023Quote: ... Average power for excitation ranged from ∼35 mW to ∼80 mW measured at the front face of the objective (CA1 imaging: Nikon 16x, 3 mm WD, 0.8 NA; DG & CA3 imaging: Leica 25x, 3 mm WD, 1.0 NA). A 512 x 796 pixel field of view (FOV ...
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bioRxiv - Bioengineering 2021Quote: ... the paraffin-embedded block tissues were sectioned at the surface with ∼7-µm thickness by a microtome (RM2235, Leica Microsystems Inc.). The thin tissue slices were stained by H&E ...
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bioRxiv - Plant Biology 2020Quote: ... The hairy roots were embedded in 7% agarose before being transversely sectioned at a thickness of 50 μm using a vibratome (Leica VT1000S, Leica, Germany). The safranin O-green staining method was used to detect the deposition and composition of lignin(Bouvier et al. ...
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bioRxiv - Developmental Biology 2022Quote: ... The fixed tails were placed in 1 × PBS solution for 2 hours and then embedded in 7% agarose gel (1 × PBS as the medium) and cut into 180 μm horizontal sections by a vibratome (LEICA VT1000S). All lizards were released at the exact location of capture in less than 4 hours.
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bioRxiv - Biochemistry 2021Quote: ... Ultrathin (70 nm) and semi-thin (300 nm) sections were generated using a Leica EM Ultracut 7 ultramicrotome (Leica, Heerbrugg, Switzerland) and sections were post-stained using 2% uranyl acetate and lead citrate respectively ...
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bioRxiv - Immunology 2023Quote: ... cut into 4 groups of 8 sequential 7-μm-thick sections separated by a depth of 150-μm using a Leica CM1860 UV cryostat (Leica Microsystems), and mounted as duplicates on Fisherbrand ProbeOn Plus slides glass slides (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2023Quote: ... The STG was then illuminated for 5-7 mins using a Texas red filter set (560 ± 40 nm wavelength; Leica Microsystems). Complete photoinactivation was confirmed when the LP membrane potential reached 0 mV ...
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bioRxiv - Physiology 2023Quote: ... Paraffin-embedded liver sections (2 μm or 7 µm for Sirius-Red staining) were processed for IHC staining using a BOND-MAX system (Leica, Germany) as previously described 54 ...
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bioRxiv - Immunology 2022Quote: ... they were paraffin-embedded and sectioned (7 µm thickness) on slides using a Leica RM2125 RTS microtome (Leica Biosystems, Concord, Canada). Slides were deparaffinized and washed using two rounds of toluene (T324-1 ...
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bioRxiv - Cancer Biology 2023Quote: Fresh frozen tissues were placed in OCT and sections were cut at 7 μm thickness and mounted onto membrane slides (Leica, 11600288). The slides were fixed in ice-cold 70% EtOH for 5 min ...
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bioRxiv - Neuroscience 2024Quote: ... The STG was then illuminated for 5 – 7 min using a Texas red filter set (560 ± 40 nm wavelength; Leica Microsystems). Complete photoinactivation was confirmed when the LP membrane potential reached 0 mV.
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bioRxiv - Cell Biology 2024Quote: ... This was followed by fracturing at -110 °C using a flat edge knife and sublimation at -105 °C and 5.6×10−7 mbar (Leica EM ACE900) for 20 s ...
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bioRxiv - Neuroscience 2024Quote: ... The STG was then illuminated for 5-7 min using a Texas red filter set (560 ± 40 nm wavelength; Leica Microsystems). Complete photoinactivation was confirmed when the LP membrane potential reached ∼0 mV (8 ...
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bioRxiv - Developmental Biology 2024Quote: ... Long bone samples were embedded in paraffin after dehydration and 5-7 μm-thick paraffin sections were cut with a microtome (RM2255, Leica Biosystems). Sections were stained with von Kossa ...