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Citations for Leica :
1651 - 1695 of 1695 citations for Mouse Fragile X Mental Retardation 1 Neighbor Protein FMR1NB ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... then sectioned coronally into 25-µm-thick slices (1:4 series of the PVN) using a Leica CM1900 cryostat (Leica Microsystems, Wetzlar, Germany). Sections were subjected to IHC using standard methods ...
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bioRxiv - Neuroscience 2021Quote: ... isogenic samples reared in the same environment were highpressure frozen (Leica HPM100 for datasets 1-5 and Leica ICE for datasets 6-8) at different stages of post-embryonic development ...
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bioRxiv - Immunology 2022Quote: ... and stained using immunohistochemistry (IHC) for collagen I (1:4000, polyclonal rabbit, lifespan biosciences, Seattle WA) via a Bond-Max autostaining system (Leica biosystems, Lincolnshire, IL). Stained kidneys were scanned at high resolution using a CRi Panoramic SCAN 40x Whole Slide Scanner (Perkin-Elmer).
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bioRxiv - Neuroscience 2022Quote: ... 1-hour each), xylene (3×, 1.5-hour each), paraffin (3 ×, 2-hours each) (Fisher, UK) in tissue processor (Leica TP1020 tissue processor, UK) and embedded in paraffin using embedding station (Leica HistoCore Arcadia H embedding station ...
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bioRxiv - Bioengineering 2019Quote: ... Confocal laser scanning microscopy of macroMs entrapment/dead cells at 4h and day 1 was performed with a Tandem confocal system (Leica TCS SP8 STED), equipped with a white light laser (WLL) ...
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bioRxiv - Neuroscience 2021Quote: ... Regularly spaced brain series (5 × 1-in-5) of 30 μm-thick frozen sections were cut in freezing microtome (SM2000R, Leica, Wetzlar, HE, Germany) in the coronal plane ...
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bioRxiv - Neuroscience 2021Quote: images were obtained using the Axio Imager 1 microscope (Zeiss, Oberkochen, Germany) and confocal images were obtained with a laser scanning confocal microscope (Leica SPE, Mannheim, Germany) associated with a Leica LAS AF interface ...
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bioRxiv - Cell Biology 2020Quote: ... samples for the projects as listed in Table 1 were cryo-immobilized by using the following high-pressure freezers: EMPACT2+RTS (Leica Microsystems, Vienna, Austria), Leica EM ICE (Leica Microsystems ...
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bioRxiv - Cancer Biology 2024Quote: ... Antigen retrieval was performed in BOND Epitope Retrieval Solution 1 (pH 6) or 2 (pH 9) (ER1, ER2, Leica Biosystems, Cat. AR9961, AR9640) at 95°C ...
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bioRxiv - Cell Biology 2023Quote: ... On day 1 after seeding the grids were incubated with 10 nm colloidal gold particles for 1 minute and rapidly frozen in liquid ethane using GP2 plunge-freezer (Leica Microsystems, Vienna, Austria). Tilt series of vitrified samples were collected using a Tecnai F20 microscope (FEI Company ...
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bioRxiv - Microbiology 2023Quote: ... Z stacks of horizontal plane images were acquired in 1 μm steps using a Leica SP8 AOBS inverted laser scanning microscope (CLSM, LEICA Microsystems, Wetzlar, Germany) at the INRAE MIMA2 platform (33) ...
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bioRxiv - Neuroscience 2022Quote: ... Brains were then frozen and coronally sectioned at a thickness of 30 μm (1:5 series) using a Leica CM1900 cryostat (Leica Microsystems, Wetzlar, Germany). Fos2A-iCreER+/-::Ai14+/- sections were mounted on gelatin-coated slides and coverslipped with Vectashield containing DAPI (cat ...
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bioRxiv - Physiology 2023Quote: ... ten H&E stained images of 1 mm2 (4,000,000 pixel2) were randomly extracted from the entire adipose tissue image using Aperio ImageScope (Leica Biosystems Imaging, Deer Park, IL) and analyzed with the MorphoLibJ collection of mathematical morphology methods and plugins using the image processing package FIJI [36] ...
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bioRxiv - Neuroscience 2023Quote: ... P14-P16 and P20-P21 pups of both sexes were placed in a perfused chamber at room temperature (22-25°C) in oxygenated Mg2+ containing ACSF (ACSF-1) medium under a Leica DMLFS microscope (Leica Microsystems Inc., USA). The recordings were performed using a coated nichrome electrode with an Iso-DAM8A World Precision Instruments amplifier (World Precision Instruments ...
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bioRxiv - Microbiology 2023Quote: ... Z-stacks of horizontal plane images were acquired in 1 μm steps using a Leica SP8 AOBS inverted laser scanning microscope (CLSM, LEICA Microsystems, Wetzlar, Germany) at the INRAE MIMA2 platform (doi.org/10.15454/1.5572348210007727E12)54.54 ...
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bioRxiv - Plant Biology 2024Quote: ... immersed in 1-Hexadecene as cryoprotectant and immediately fixed by high pressure freezing (HPF) using the Leica EM ICE (Leica Microsystems GmbH, Germany). Freeze substitution and embedding of the HPF-fixed samples were conducted in a temperature-controlled device ...
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bioRxiv - Neuroscience 2024Quote: ... together with Opal 520/570/690 Fluorophore Reagent pack detection (1:1000, Cat #FP1487001KT/FP1488001KT/FP1497001KT) on the Leica BOND RX Fully Automated Research Stainer (Leica, Buffalo Grove, IL) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: Grids were screened for cells with ASC/caspase-1 speck by light microscopy using a cryo-FM microscope (Leica EM Cryo CLEM, Leica Microsystems), equipped with a cryo-stage ...
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bioRxiv - Genomics 2020Quote: ... individuals were lethally anaesthetized using benzocaine bath (150 mg.L−1) and a lateral photograph was taken using a digital fixed camera (Leica Microsystems; Figure 1B and C). Microbiome and host sampling consisted in gentle fish skin smears with sterile swabs ...
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bioRxiv - Bioengineering 2021Quote: ... or Toluidine Blue Stain, 1% w/v (Ricca Chemical, Arlington, TX) and scanned with a Leica Aperio ScanScope® CS2 (Leica, Buffalo Brove, IL) system ...
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bioRxiv - Molecular Biology 2020Quote: ... 4 μl of this mixture was back side blotted for 6 s at relative humidity 98% and temperature 19°C on a glow-discharged holey carbon grid (CF-2/1-3C, Protochips) before plunge-freezing in liquid ethane (Leica EM GP2 automatic plunger). Dose-symmetrical tilt series acquisition 51 was performed on an FEI Titan Krios electron microscope operated at 300 kV using a Gatan Quantum energy filter with a slit width of 20 eV and a K2 or K3 direct detector operated in counting mode ...
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bioRxiv - Neuroscience 2020Quote: ... the brain of each mouse extending from anterior to the DG to the cerebellum (from 0.22 to −5.34 μm from Bregma) was sectioned at 30 μm in a 1:9 series using a freezing microtome (Leica SM 2000 R Sliding Microtome). Sections were stored in 1xPBS with 0.01% sodium azide at 4°C until processing for IHC ...
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bioRxiv - Cancer Biology 2022Quote: Immunohistochemical staining was carried out on 1 μm tissue section in automated immunostainers (Discovery®XT, Roche, Penzberg, Germany, and Leica Bond, Wetzlar, Germany) using the streptavidin-peroxidase method ...
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bioRxiv - Plant Biology 2019Quote: ... the cotyledons were stained with 1 µg/ml propidium iodide and observed under a Confocal microscope (Carl Zeiss LSM710, Carl Zeiss LSM880 or Leica TCS SP8). For excitation ...
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bioRxiv - Cancer Biology 2021Quote: ... Agilent M087601-2, used at a dilution of 1/50 for 15mins at room temperature. HIER was performed on-board using Leica ER1 solution (low pH) for 20 minutes ...
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bioRxiv - Molecular Biology 2021Quote: ... the thecal plates of the dinoflagellates were stained with the fluorescent dye Calcofluor white M2R at a final concentration of 10–20 mg mL−1 (Fritz & Triemer, 1985) and examined under epifluorescence (lamp 50W) using a Leica DM IRB (Leica Microsystemps GMbH, Wetzlar, Germany) inverted microscope ...
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bioRxiv - Cancer Biology 2022Quote: Tissue sections were incubated for 1 hour in primary antibodies and detected using the BOND Polymer Refine Detection System (DS9800; Leica Biosystems, Buffalo Grove, IL) in accordance with the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... Sections were then stained for Ly6G (clone 1A8) at a previously optimized dilution (1/60000) for 30 min at room temperature with the autostainer Bond Rx (Leica Microsystems GmbH, Wetzlar, Germany). The IHC sections were visualised with 3,3’Diaminobenzidine (DAB) ...
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bioRxiv - Cancer Biology 2023Quote: 4 µm FFPE tissue sections were baked for 1 hour at 62°C in vertical slide orientation with subsequent prestaining of deparaffinization (Leica, Bond Dewax Solution, AR9222) and 35 minutes of Bond ER2 (Leica ...
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bioRxiv - Molecular Biology 2023Quote: ... Larvae were then positioned and oriented laterally to acquire two z-stacks of 30 optical sections (ΔZi, Zi-1 = 683.46μm) of the gastrointestinal tract using a 4X objective (Leica HC PL FLUOTAR 4X/0.13 DRY) at 10 msec exposures ...
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bioRxiv - Cancer Biology 2023Quote: Tissues sections were incubated for 1 h in primary antibodies and detected using the BOND Polymer Refine Detection System (DS9800; Leica Biosystems, Buffalo Grove, IL) in accordance with the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... Lewy bodies were counted on sections from the described regions following immunohistochemistry for alpha-synuclein (Leica, cat# ncl-l-asyn, 1:100-400 dilution). Of note ...
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bioRxiv - Immunology 2023Quote: ... Frozen brains were then embedded in Epredia™ M-1 Embedding Matrix (Thermo Ficher Scientific) and cut in coronal sections using a cryostat (Leica CM3050S, Mannheim, Germany), and mounted on slides for either fluorescence immunocytochemistry (IF ...
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bioRxiv - Plant Biology 2024Quote: ... Semi-thin sections at 1 µm were prepared using the resin-embedded buds using a glass knife on an EM UC6 ultramicrotome (Leica Microsystems GmbH, Wetzlar, Germany). Sections were mounted on glass slides ...
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bioRxiv - Plant Biology 2024Quote: ... 4 DAT seedlings were treated with 1 M PEP1 for 24h before they were imaged using confocal miscrscopes (Leica TCS SP8 and Zeiss LSM700). To visualize the GFP protein ...
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bioRxiv - Molecular Biology 2024Quote: ... For scoring (graphs Figure 1) the percentage of cells expressing the chromatin markers were scored manually under an epi-fluorescence microscope (Leica DM6000 Leica microsystems, Germany).
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bioRxiv - Neuroscience 2024Quote: Immuno-stained anti-rabbit Iba-1 brain sections were imaged at a 40x oil objective using a confocal imaging system (Leica Microsystems, Deerfield, IL, USA) to further investigate microglia structure and morphology ...
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bioRxiv - Bioengineering 2021Quote: ... Samples were longitudinally cut into slices with a thickness of 1 mm and observed by confocal laser scanning microscopy (CLSM, Leica TCSSP8 MP, Leica microsystems, GER). Semi-quantitative analysis was conducted through the assay of the horizontal and vertical fluorescence intensity of the hybrid layer ...
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bioRxiv - Cell Biology 2019Quote: Quantification of protein by Western blotting was carried out using standard techniques and the following primary and secondary antibodies: anti-α-SMA (SMA-R-7-CE, Leica Biosystems, dilution: 1:100), anti-COL1 (1310-01 ...
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bioRxiv - Cancer Biology 2021Quote: ... Optimal primary antibody stripping conditions between rounds in the seven-color assay were performed following 1 cycle of tyramide deposition followed by heat-induced stripping (see below) and subsequent chromogenic development (Leica Bond Polymer Regine Detection DS9800) with visual inspection for chromogenic product with a light microscope by a senior pathologist (T.J.H.) ...
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bioRxiv - Molecular Biology 2023Quote: ... Zi-1 = 75.61μm) of pancreatic beta cells using the microscope’s TXR filter and a 40X objective (Leica HCX APO L U-V-I 0.80 W), at 20 msec exposures ...
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bioRxiv - Cancer Biology 2023Quote: ... Optimal primary antibody stripping conditions between rounds in the seven-color assay were performed following 1 cycle of tyramide deposition followed by heat-induced stripping (see below) and subsequent chromogenic development (Leica Bond Polymer Regine Detection DS9800) with visual inspection for chromogenic product with a light microscope (TH) ...
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bioRxiv - Molecular Biology 2023Quote: ... three z-stacks of 75 optical sections each (ΔZi, Zi-1 = 151.75μm) were acquired of the bigger liver lobe using a 20X objective (Leica HCX APO L U-V-I 0.80 W). One z-stack was in bright field and acquired at 20 msec exposures ...
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bioRxiv - Plant Biology 2022Quote: ... high-light illumination of the cells was achieved by exposing the cuvette to white light set to an intensity of 620 μmol photons m-2 s-1 (Leica KL1500 LCD, peak 648 nm, FWHM 220 nm), chosen as the minimal light level necessary to saturate photosynthetic reactions and induce qE activity at the onset of illumination ...
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bioRxiv - Developmental Biology 2019Quote: ... samples were mounted for imaging on a Leica SP5 (siRNA experiments) or SP8 (DLD-1 experiments and experiments to assess subcellular localization) confocal microscope with an oil immersion objective (Leica HCX PL APO lambda blue, 63X, N.A. 1.4). Z-stacks were taken every 1 µm.