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Citations for Leica :
1551 - 1600 of 2056 citations for 5 Pyrimidinecarbonitrile 1 2 3 4 tetrahydro 4 oxo 6 phenyl 2 thioxo since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... The cells were resuspended in 80 µl of the M solution and 5 µl were used for fluorescence microscopy observation under the Leica TCS SP8 confocal microscope (Leica, Germany). The data was analyzed using the Leica Application Suite X imaging and analysis software (Leica ...
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bioRxiv - Microbiology 2022Quote: ... The target probe was validated on reproductive tracts from mice experimentally inoculated with Chlamydia muridarum strain Nigg.5 Slides were stained on an automated stainer (Leica Bond RX, Leica Biosystems) with RNAscope 2.5 LS Assay Reagent Kit-Red (322150 ...
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bioRxiv - Neuroscience 2019Quote: ... Representative images of further experiments investigating neurite outgrowth in differentiated CTX0E16 cells were acquired using a Leica SP-5 confocal microscope with a 100x oil-immersion objective (Leica microsystems). Z-stacks of 5 individual cells per condition across three biological replicates were obtained for statistical data analysis ...
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bioRxiv - Neuroscience 2020Quote: ... and 25 mM dextrose) with bubbling 95% O2 and 5% CO2 using a vibratome (VT1000 S, Leica Microsystems, Buffalo Grove, IL). Acute bilateral fastigial nuclei were quickly excised with a sharp knife from 2 or 3 cerebellar slices and were then enzymatically digested with cysteine (2 mM)-supplemented papain (40 U/ml ...
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bioRxiv - Developmental Biology 2021Quote: ... For immunohistochemistry 5 μm thick serial sections were cut at −23°C using a Leica CM3050 cryostat (Leica Microsystems, Wetzlar, Germany) and collected in Superfrost® Plus slides (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: All experiments were imaged using a Leica SP-5 confocal microscope using a Plan-Apochromatic 63x 1.40 NA oil-immersion objective (Leica microsystems, 506210). Fluorophores were excited using a 100 mW Ar laser (458 ...
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bioRxiv - Plant Biology 2019Quote: ... The root segments were embedded in agarose 5% and cut into 25 μm radial sections with a Leica VT1200S Leica vibratome (Leica Microsystems). Sections were categorized according to their distance (100 ...
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bioRxiv - Physiology 2021Quote: ... The lung tissue sections of 3-5 micron were stained with H&E stain and were microscopically analyzed using microscope (Make: Leica DM1000 LED; Camera: Leica MC170HD). The images were taken with 10X objective lens (Magnification X 100 ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were mounted on an aluminum stub with a silver lacquer and sputtercoated with a 5 nm platinum layer using EM ACE600 (Leica Microsystems). Acquisitions were performed using a GeminiSEM 500 (Zeiss).
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bioRxiv - Cell Biology 2022Quote: ... lensectomy and sclerectomy were performed and retinas embedded in 5% agar and 150 µm sections made using a fully automated VT1200S vibrating blade microtome (Leica Biosystems). Sections were cultured in retinal media (DMEM extra (Sigma ...
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bioRxiv - Microbiology 2022Quote: ... at 5 µm in thickness with a tungsten carbide blade (SL-T30, SECTION LAB Co. Ltd) using a cryostat system (Leica CM1850). The undecalcified sections attached to the adhesive film were immerged in 100% ethanol for 30 sec to remove the compound and air-dried ...
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bioRxiv - Neuroscience 2022Quote: ... Their brains were quickly dissected out and stuck on a 5 mm agar stage before being mounted on a vibratome (Leica VT1200S) equipped with a sapphire blade ...
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bioRxiv - Neuroscience 2022Quote: ... and Homo (N=5 males) Gcg-Cre rats from the Janvier colony were cut into 50 μm coronal slices using a cryostat (Leica CM3050S). The cNTS was then microdissected using biopsy punches (Integra Miltex ...
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bioRxiv - Physiology 2022Quote: ... then incubated with serum-free protein block for 5 minutes (Novocastra Protein Block Protein Block Serum Free, Leica Biosysems, cat# RE7102), followed by incubation with rabbit anti-FABP4 polyclonal antibody (adipose ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were mounted on an aluminum stub with a silver lacquer and sputtercoated with a 5 nm platinum layer using EM ACE600 (Leica Microsystems). Acquisitions were performed using a GeminiSEM 500 (Zeiss).
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and 5% H2O) under liquid nitrogen and placed in an automatic freeze substitution system (Leica AFS2, Leica Microsystems, precooled to −90 ℃) for 24 h ...
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bioRxiv - Cell Biology 2024Quote: ... Paraffin-embedded WAT was sliced into 5-μm sections using a ThermoScientific HM 355S Automatic Microtome and mounted on Surgipath X-tra Microscope Slides (Leica Biosystems).
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bioRxiv - Neuroscience 2024Quote: ... the lumbar spinal cord from L3-5 was mounted on an agarose mold and the dorsal halve was micro dissected on a vibratome (Leica Microsystems). Next ...
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bioRxiv - Evolutionary Biology 2023Quote: ... LEGE 07298 and Phormidesmis priestleyi ULC 007 stained with either calcein or 5-CF were acquired with an inverted confocal laser scanning microscope (CLSM; Leica SP8) using a HC PL APO x63/1.40 oil objective ...
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bioRxiv - Synthetic Biology 2023Quote: ... The frozen samples were transferred to 1% Osmium tetroxide and 0.1% uranylacetate in acetone and freeze substituted using a Leica EM AFS2 freeze substitution processor at – 90°C with a slow warm up of 5°C per hour up to 0°C (Leica Microsystems). As osmium becomes increasingly reactive at higher temperatures we removed the samples from the AFS2 at 0°C to preserve the “crispiness” of the staining ...
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Apical expansion of calvarial osteoblasts and suture patency is dependent on graded fibronectin cuesbioRxiv - Developmental Biology 2023Quote: ... Confocal images were captured at 63X oil immersion objective on Leica STELLARIS 5 (Leica; HC PL APO 63x/1.40 OIL CS2) using Application suite X software ...
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bioRxiv - Cell Biology 2023Quote: ... All confocal images were acquired using the Leica TCS SP8 or Stellaris 5 confocal microscope with the Leica LAS X software (Leica Microsystems). Following objectives were used with the Leica TCS SP8 ...
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bioRxiv - Cell Biology 2023Quote: ... Slices were imaged with a laser scanning confocal Stellaris 5 or TCS SP8 gSTED equipped with a white light laser and a 405-diode ultraviolet laser (Leica Microscope). Image acquisition was done using the LAS-X software in accordance with the Nyquist sampling sequential mode by exciting the fluorophores and collecting their signal with hybrid detectors (HyDs).
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bioRxiv - Cancer Biology 2023Quote: ... at a final concentration of 5 µg/mL for 20 minutes at 37 °C prior to live cell imaging by epifluorescence (Leica DMI6000B). Cells were counted as DAPI-positive if the nucleus showed bright staining and as apoptotic if there was characteristic nuclear fragmentation ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were placed in a 37°C chamber incubator supplemented with 5% CO2 mounted on Leica TCS SP8X microscope system (Leica Microsystems).
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bioRxiv - Plant Biology 2023Quote: Lengths of root RAM and EZ regions and root hairs in 5-d-old seedlings grown on vertical MS medium without sucrose were measured under a Leica microscope (Leica DM5000). The data were analyzed using the ImageJ software (http://rsb.info ...
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bioRxiv - Neuroscience 2023Quote: ... The STG was then illuminated for 5-7 mins using a Texas red filter set (560 ± 40 nm wavelength; Leica Microsystems). Complete photoinactivation was confirmed when the LP membrane potential reached 0 mV ...
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bioRxiv - Microbiology 2023Quote: ... as described above, the cells were incubated (37°C, 5% CO2) inside the Mica Microhub system using the internal incubator (Leica microsystems). Videos were analysed using the complementary software (Leica microsystems).
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bioRxiv - Neuroscience 2023Quote: ... the brain was briefly submerged in ice cold cut solution bubbled with carbogen (95% oxygen, 5% CO2) and then sectioned into 300μm sagittal sections (Leica VT1200S vibratome) in bubbled ice-cold cut solution ...
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bioRxiv - Neuroscience 2024Quote: ... The STG was then illuminated for 5 – 7 min using a Texas red filter set (560 ± 40 nm wavelength; Leica Microsystems). Complete photoinactivation was confirmed when the LP membrane potential reached 0 mV.
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bioRxiv - Plant Biology 2024Quote: ... mounted in H2O on a slide and imaged with a 63x glycerol immersion objective on a Leica TCS SP8 microscope or a Leica Stellaris 5 microscope (Leica Microsystems). On the SP8 we imaged using an Argon laser at 20% power ...
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bioRxiv - Biophysics 2024Quote: ... The removed brain was washed thrice with PBS (5 min each, shaking in a shaker) and subsequently sectioned into 100 µm thick sections using a vibratome (Leica, VT1000S). The resulting brain sections were subjected to label-free CARS and SHG imaging.
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bioRxiv - Biophysics 2024Quote: ... we deposited approximately 5 nm of carbon to the nanopipettes using thermal evaporation (CED 030, BalTec) with double-stranded carbon thread (Leica Microsystems). We imaged the nanopipettes by scanning electron microscopy (LEO 1530 VP ...
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bioRxiv - Neuroscience 2024Quote: ... The lumbar spinal cord (L2-5) was sectioned transversely (300-350 µm) with dorsal roots attached using a vibrating microtome (Leica Microsystems). Slices were next transferred to an artificial cerebrospinal fluid (ACSF) ...
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bioRxiv - Developmental Biology 2024Quote: ... before being embedded in 5% low-melting agarose and sectioned into 100-400 μm-thick slices using a Vibratome (Leica, VT1200). Slices were blocked and permeabilized using PBST supplemented with 5% donkey serum and 0.3% Triton X at room temperature for 30 minutes ...
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bioRxiv - Cell Biology 2020Quote: ... CD56 (1:200, Leica Biosystems), or CD163 (1:200 ...
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bioRxiv - Developmental Biology 2019Quote: ... p63 (1:50, Leica Biosystems), CK8/18 (1:50 ...
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bioRxiv - Developmental Biology 2019Quote: ... p63 (1:50, Leica Biosystems), CK8/18 (1:50 ...
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bioRxiv - Developmental Biology 2020Quote: Pregnant female mice (E3.5 and E9.5) were sacrificed by cervical dislocation for embryo dissections under a dissecting microscope (M165C or M80, Leica Microsystems; Wetzlar, Germany) as previously described (Behringer et al ...
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bioRxiv - Immunology 2021Quote: ... Fixed tissue samples were then embedded in paraffin and 5 µm thick sections were cut at a rotary microtome (Leica, Wetzlar, Germany). After a 10 min incubation in PBS ...
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bioRxiv - Neuroscience 2019Quote: Confocal images of double-stained neurons were acquired with a Leica SP-5 confocal microscope using a 63x oil-immersion objective (Leica, N.A. 1.4) as a z-series ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: we measured the teleochrysalid abdomen surface area (Troscianko, 2014) from high-resolution photos (Leica M50, 5 MP HD Microscope Camera Leica MC170 HD).
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bioRxiv - Neuroscience 2020Quote: ... Coronal brain slices containing the hippocampal formation (400 μm thick) were prepared in the ice-cold cutting solution bubbled with 95% O2/5% CO2 using Vibratome VT1200S (Leica Microsystems, Germany). The slices were transferred into an interface chamber in ACSF containing (in mM) ...
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bioRxiv - Cancer Biology 2019Quote: ... a minimum of 5 images were taken of cells per experiment using a 20x water objective on an inverted confocal microscope (Leica TCS SP8). Using phalloidin staining and Image J software ...
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bioRxiv - Neuroscience 2021Quote: ... The brains were allowed to rest in cold oxygenated (95% O2/5% CO2) aCSF and then sliced coronally using a vibratome (Leica VT1000 S). Brain slices (400-μm thick sections ...
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bioRxiv - Genetics 2022Quote: ... H&E staining was performed on 5 µm sections from paraffin-embedded retinas according to standard protocols for light microscopy and photomicrography (Leica, Wetzlar, Germany).
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bioRxiv - Cell Biology 2020Quote: ... Cells were then imaged at 37°C in a chamber supplemented with humidified 5% CO2 through white light laser confocal microscope TCS SP8 (Leica, Wetzlar, Germany). Movie S1 was processed by Bitplane-Imaris 9.5 (Andor Technology ...
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bioRxiv - Biophysics 2020Quote: ... stage of growth were imaged every 10 minutes for 12 hours in a customized incubator (37°C, 5% CO2, and 95% humidity) on a confocal microscope (Leica, TCL SP8), with acquisition of fluorescent and bright-field image z-stacks (Fig ...
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bioRxiv - Bioengineering 2021Quote: ... The samples washed in PBS (3x 5 min) and embedded in Mowiol prior to confocal laser scanning microscope analysis (Leica TCS SP8X). Collagen fiber organization was determined as described in the supplementary information (supplementary Fig.S2-4 and supplementary Eq.S1.).
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bioRxiv - Immunology 2021Quote: ... Lung tissue was embedded in Optimal Cutting Temperature Compound (Tissue-Tek) and 5 μm sections were cut on CM1950 Cyrostat (Leica, Weltzlar, Germany). Sections were blocked with 1% Rat serum and 1% FcBlock in PBS for 1 hr ...