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Citations for Leica :
1451 - 1500 of 1523 citations for IL 1 beta Rat since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... HMC-1 cells were loaded into membrane carriers and were high-pressure frozen in a Leica EM PACT1 (Leica microsystems, Wetzlar, Germany). T ...
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bioRxiv - Microbiology 2020Quote: ... Max projections of confocal z-stacks (25 z steps spaced 1 μm) were acquired on a SP5 resonant APD (Leica, Wetzlar, Germany) at 1.7x zoom using a 63x glycerol objective (numerical aperture 1.4).
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bioRxiv - Cell Biology 2021Quote: ... Freeze substitution was performed over 3 d at −90°C in anhydrous acetone containing 1% OsO4 and 0.1% uranyl acetate using an automatic freeze substitution machine (EM AFS, Leica Microsystems, Vienna, Austria). Epon/Araldite infiltrated samples were then embedded in a thin layer of resin and polymerized for 3 d at 60°C ...
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bioRxiv - Cancer Biology 2022Quote: ... and incubated overnight at 4°C in 0.05% Digitonin-Antibody buffer containing a rabbit anti mouse-p53 Ab (1:100 Leica, NCL-p53-CM5p). Samples incubated with rabbit IgG (1:150 ...
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bioRxiv - Neuroscience 2021Quote: Every 4th brain section was imaged as a Z-stack (1 μm step size) using either the Zeiss LSM 800 (Zen, v2.6.76) or Leica SP8 confocal microscope (Leica Application Suite X, v3.5.6.21594) at 20x magnification ...
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bioRxiv - Cancer Biology 2021Quote: ... and red tdTomato/CMDiI channels and were recorded as approximately 1 x 4 stitches at 10x magnification using a Leica sp8 confocal microscope (Leica, Wetzlar, Germany). Consecutive stitch sequences were processed into a single image using Fiji 33 using the plugin by Preibisch et al. ...
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bioRxiv - Bioengineering 2020Quote: ... eyes were fixed in 4% paraformaldehyde (w/v) in PBS for 1 hour and embedded in optimal cutting temperature compound (Leica Biosystems, Germany) and stored at -80C until cryosectioning ...
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bioRxiv - Plant Biology 2020Quote: ... Light scattering changes in the presence of 50 μM MV were induced by applying red light (1,000 μmol m−2 s−1, Leica KL 1500 LED). Scattering changes were measured first in dark for 100 s ...
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bioRxiv - Cell Biology 2020Quote: ... Freeze substitution was performed over 3 days at –90 °C in anhydrous acetone containing 1 % OsO4 and 0.1 % uranyl acetate using an automatic freeze substitution machine (EM AFS, Leica Microsystems, Vienna, Austria) Serial semi-thick sections (300nm ...
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bioRxiv - Developmental Biology 2020Quote: ... Finally, embryos were cleared in BABB (benzyl alcohol: benzyl benzoate, 1:2) solution and vizualized under light microscope (Leica MZ6 Infinity1 stereomicroscope).
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bioRxiv - Cell Biology 2021Quote: ... Freeze substitution was performed over 3 d at -90 °C in anhydrous acetone containing 1 % OsO4 and 0.1 % uranyl acetate using an automatic freeze substitution machine (EM AFS, Leica Microsystems, Vienna, Austria). Epon/Araldite infiltrated samples were flat embedded in a thin layer of resin ...
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bioRxiv - Bioengineering 2022Quote: ... The samples were stored at −80°C for less than 1 week prior to sectioning (10 μm thick) using a CM1950 Cryostat (Leica, Wetzlar, Germany). Tissue sections were fixed and permeabilized in 1:1 methanol:acetone solution at −20°C ...
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bioRxiv - Cancer Biology 2024Quote: ... The slides were heated at 60°C for 1 h in an oven before H&E staining in the autostainer (Leica Autostainer XL). IHC was performed using a detection kit (Vector Laboratories ...
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bioRxiv - Cell Biology 2023Quote: ... 30% sucrose in PBS (2:1 v/v) over-night (O/N) at 4°C with gentle shaking and frozen in OCT (Leica Surgipath, FSC22), using plastic molds (Leica Surgipath ...
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bioRxiv - Molecular Biology 2023Quote: ... blotted with Whatman 1 filter paper and vitrified into liquid ethane at -180°C using a Leica GP2 plunger (Leica microsystems, Austria). Frozen grids were transferred onto a Talos 200C Electron microscope (FEI ...
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bioRxiv - Cancer Biology 2023Quote: ... Slides were immediately washed in running distilled water for 1 min prior to counterstaining cell nuclei with diluted Gill’s Hematoxylin (Leica Biosystems, Wetzlar, Germany). Slides were washed under running distilled water ...
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bioRxiv - Biochemistry 2023Quote: ... 3µL of the samples were dispensed onto glow discharged Quantifoil holey carbon grids (Cu R2/1 or R2/2) using a Leica GP plunger (Leica Microsystems, Austria) maintained at 90% humidity and 10°C ...
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bioRxiv - Plant Biology 2023Quote: 50 µm-thick cross sections were prepared from apical and basal internodes of Arabidopsis inflorescence stems at stage 1 (Altamura et al., 2001) by a vibrating blade microtome (Leica VT1200 S), stained with a 0.05% (w/v ...
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bioRxiv - Cell Biology 2023Quote: ... Imaging was performed using Leica DMI8 confocal microscope and HC PL APO CS2 20x objective (excitation, 488 nm, emission, 498 nm - 550 nm, pinhole size of 1 Airy Unit) (Leica Microsystems, Germany), pixel dimensions 0.48 μm px-1 ...
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bioRxiv - Biochemistry 2023Quote: 3 µl of the individual complexes were applied onto glow-discharged Quantifoil holey carbon grids (Cu R2/1 or R2/2) and vitrified in liquid ethane (−181 °C) using a Leica GP plunger (Leica Microsystems, Austria) maintained at 90% humidity and 10 °C ...
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bioRxiv - Cancer Biology 2023Quote: ... Primary antibody incubation at 1:250 dilution for 30 minutes was followed by detection using the BOND™ Polymer Refine Detection System (DS9800, Leica Biosystems) as per manufacturer’s instructions.
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Apical expansion of calvarial osteoblasts and suture patency is dependent on graded fibronectin cuesbioRxiv - Developmental Biology 2023Quote: ... the nuclei were stained with DAPI (1:2000) and the images were captured with 63X oil immersion objective by the confocal microscope (Leica STELLARIS 5). The measurements related to cell shape analysis of the nuclei in apical ...
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bioRxiv - Neuroscience 2023Quote: ... were rinsed in PBS and embedded in 3% agarose containing 1% Tween 20 and sectioned at 300 µm in the frontal plane with a vibratome (Leica VT 1200S). Under a stereomicroscope (Olympus SZX7) ...
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bioRxiv - Bioengineering 2023Quote: ... Slides were let dry for at least 1 h prior to imaging at the microscope (Leica DM 2000 LED fitted with a Leica DFC450c camera). Ten images from different regions of the slide were acquired and the number of invaded cells counted using custom-made software further described in the next section ...
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bioRxiv - Plant Biology 2024Quote: ... Root tissue was then embedded in 5% low-melting point agarose (agarose dissolved in 1× PBS) and the embedded blocks were sectioned using a Leica RM2255 microtome (Leica, Nussloch, Germany). The root tissue sections were separated from agarose by rinsing ...
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bioRxiv - Molecular Biology 2023Quote: ... blotted with Whatman 1 filter paper and vitrified into liquid ethane at –180 °C using a Leica GP2 plunger (Leica microsystems, Austria). Frozen grids were transferred onto a Talos 200C Electron microscope (FEI ...
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bioRxiv - Molecular Biology 2024Quote: ... For scoring (graphs Figure 1) the percentage of cells expressing the chromatin markers were scored manually under an epi-fluorescence microscope (Leica DM6000 Leica microsystems, Germany).
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bioRxiv - Neuroscience 2024Quote: ... 30μm serial sagittal sections were collected from Cx3cr1GFP/+ animals in a tempero-medial manner (1 in 12 series) using a vibratome (Leica VT 1200S) and kept as free-floating sections in ice-cold PBS ...
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bioRxiv - Microbiology 2024Quote: ... followed by fine trimming of an initial region of interest (ROI) spanning 2 x 2 x 1 mm using a glass knife and Leica UC7 ultramicrotome (Leica Microsystems, Austria). The trimmed block was then mounted on an ultramicrotome stub using conductive silver epoxy (SPI ...
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bioRxiv - Physiology 2024Quote: ... an area of each section (1 section/mouse) was acquired at 200x magnification using a confocal laser scanning microscope (Leica TCS SP8). Sequential scanning was employed using the 488 nm laser to visualize Alexa 488 and the 405 nm UV lasers to visualize DAPI staining ...
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bioRxiv - Neuroscience 2021Quote: ... then sectioned coronally into 20-μm-thick slices (1:6 series of the PVN) using a Leica CM1900 cryostat (Leica Microsystems, Wetzlar, Germany). Immunofluorescence staining was performed on brain sections derived from P10 male tdTomato-Crh (Crh-IRES-Cre;Ai14 ...
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bioRxiv - Immunology 2021Quote: ... z-stacks were generated with a 20x objective at a z-step of 1 µm using a SP8 laser scanning confocal microscope (Leica Biosystems, Nußloch, Germany). Composite images were generated using ImageJ with Fiji distribution [31].
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bioRxiv - Neuroscience 2020Quote: ... Cryosections were prepared from tissue embedded in a 2:1 mixture of 30% sucrose: OCT (Sakura Finetek, catalog #4583) onto X-tra slides (Leica Microsystems, catalog #3800200). Tissue sections were dried ...
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bioRxiv - Neuroscience 2021Quote: ... then sectioned coronally into 25-µm-thick slices (1:4 series of the PVN) using a Leica CM1900 cryostat (Leica Microsystems, Wetzlar, Germany). Sections were subjected to IHC using standard methods ...
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bioRxiv - Neuroscience 2021Quote: ... isogenic samples reared in the same environment were highpressure frozen (Leica HPM100 for datasets 1-5 and Leica ICE for datasets 6-8) at different stages of post-embryonic development ...
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bioRxiv - Neuroscience 2022Quote: ... 1-hour each), xylene (3×, 1.5-hour each), paraffin (3 ×, 2-hours each) (Fisher, UK) in tissue processor (Leica TP1020 tissue processor, UK) and embedded in paraffin using embedding station (Leica HistoCore Arcadia H embedding station ...
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bioRxiv - Bioengineering 2019Quote: ... Confocal laser scanning microscopy of macroMs entrapment/dead cells at 4h and day 1 was performed with a Tandem confocal system (Leica TCS SP8 STED), equipped with a white light laser (WLL) ...
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bioRxiv - Neuroscience 2021Quote: ... Regularly spaced brain series (5 × 1-in-5) of 30 μm-thick frozen sections were cut in freezing microtome (SM2000R, Leica, Wetzlar, HE, Germany) in the coronal plane ...
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bioRxiv - Neuroscience 2021Quote: images were obtained using the Axio Imager 1 microscope (Zeiss, Oberkochen, Germany) and confocal images were obtained with a laser scanning confocal microscope (Leica SPE, Mannheim, Germany) associated with a Leica LAS AF interface ...
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bioRxiv - Cell Biology 2020Quote: ... samples for the projects as listed in Table 1 were cryo-immobilized by using the following high-pressure freezers: EMPACT2+RTS (Leica Microsystems, Vienna, Austria), Leica EM ICE (Leica Microsystems ...
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bioRxiv - Cancer Biology 2024Quote: ... Antigen retrieval was performed in BOND Epitope Retrieval Solution 1 (pH 6) or 2 (pH 9) (ER1, ER2, Leica Biosystems, Cat. AR9961, AR9640) at 95°C ...
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bioRxiv - Cell Biology 2023Quote: ... On day 1 after seeding the grids were incubated with 10 nm colloidal gold particles for 1 minute and rapidly frozen in liquid ethane using GP2 plunge-freezer (Leica Microsystems, Vienna, Austria). Tilt series of vitrified samples were collected using a Tecnai F20 microscope (FEI Company ...
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bioRxiv - Microbiology 2023Quote: ... Z stacks of horizontal plane images were acquired in 1 μm steps using a Leica SP8 AOBS inverted laser scanning microscope (CLSM, LEICA Microsystems, Wetzlar, Germany) at the INRAE MIMA2 platform (33) ...
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bioRxiv - Neuroscience 2022Quote: ... Brains were then frozen and coronally sectioned at a thickness of 30 μm (1:5 series) using a Leica CM1900 cryostat (Leica Microsystems, Wetzlar, Germany). Fos2A-iCreER+/-::Ai14+/- sections were mounted on gelatin-coated slides and coverslipped with Vectashield containing DAPI (cat ...
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bioRxiv - Neuroscience 2023Quote: ... P14-P16 and P20-P21 pups of both sexes were placed in a perfused chamber at room temperature (22-25°C) in oxygenated Mg2+ containing ACSF (ACSF-1) medium under a Leica DMLFS microscope (Leica Microsystems Inc., USA). The recordings were performed using a coated nichrome electrode with an Iso-DAM8A World Precision Instruments amplifier (World Precision Instruments ...
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bioRxiv - Microbiology 2023Quote: ... Z-stacks of horizontal plane images were acquired in 1 μm steps using a Leica SP8 AOBS inverted laser scanning microscope (CLSM, LEICA Microsystems, Wetzlar, Germany) at the INRAE MIMA2 platform (doi.org/10.15454/1.5572348210007727E12)54.54 ...
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bioRxiv - Plant Biology 2024Quote: ... immersed in 1-Hexadecene as cryoprotectant and immediately fixed by high pressure freezing (HPF) using the Leica EM ICE (Leica Microsystems GmbH, Germany). Freeze substitution and embedding of the HPF-fixed samples were conducted in a temperature-controlled device ...
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bioRxiv - Immunology 2021Quote: Grids were screened for cells with ASC/caspase-1 speck by light microscopy using a cryo-FM microscope (Leica EM Cryo CLEM, Leica Microsystems), equipped with a cryo-stage ...
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bioRxiv - Genomics 2020Quote: ... individuals were lethally anaesthetized using benzocaine bath (150 mg.L−1) and a lateral photograph was taken using a digital fixed camera (Leica Microsystems; Figure 1B and C). Microbiome and host sampling consisted in gentle fish skin smears with sterile swabs ...
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bioRxiv - Molecular Biology 2020Quote: ... 4 μl of this mixture was back side blotted for 6 s at relative humidity 98% and temperature 19°C on a glow-discharged holey carbon grid (CF-2/1-3C, Protochips) before plunge-freezing in liquid ethane (Leica EM GP2 automatic plunger). Dose-symmetrical tilt series acquisition 51 was performed on an FEI Titan Krios electron microscope operated at 300 kV using a Gatan Quantum energy filter with a slit width of 20 eV and a K2 or K3 direct detector operated in counting mode ...