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Citations for Leica :
101 - 150 of 162 citations for Siglec 15 Human HEK293 mFc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... front blotted for 3-4 seconds with 1 mm additional movement (95% humidity at 15°C) before being plunged into liquid ethane using an EM GP2 automatic plunge freezer (Leica). The cryo-EM data sets of NbE-µOR-Fab module complex were acquired on a Thermo Scientific Talos Arctica Cryo-TEM at an accelerating voltage of 200 kV ...
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bioRxiv - Microbiology 2024Quote: ... Quantifoil grids consisting of a 1.2/1.3 μm holey carbon film on 300 gold mesh were glow discharged at 15 mA for 1 minute with an EM ACE200 glow discharger (Leica). Just before vitrification ...
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bioRxiv - Plant Biology 2019Quote: ... Cross-sections of the embedded roots (with a thickness of approx. 15 µm) were prepared using an RM2255 microtome (Leica, Germany). For each replication ...
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bioRxiv - Immunology 2022Quote: ... Heat-induced epitope retrieval was conducted by heating slides to 95°C for 15 minutes in EDTA-based ER2 buffer (Leica Biosystems). The SARS-CoV-2 probe (catalog number 848568 ...
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bioRxiv - Neuroscience 2021Quote: ... Brains were dehydrated in sucrose (15% and 30%, two days) at 4°C and sectioned at 40 μm thicknesses on a cryostat microtome (Leica, CM3050s). The brain slices were collected into three sets ...
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bioRxiv - Neuroscience 2021Quote: ... 20 μm thick brain slices for fluorescence in situ hybridization were cut from fresh frozen brains at -15 °C to -25 °C using a Leica CM1850 cryostat (Leica Biosystems). Slices were mounted on Superfrost Plus microscope slides (Fisher Scientific ...
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bioRxiv - Neuroscience 2019Quote: ... snapshots of the worm were taken at 15 frames per second for 30 s using a IMAGINGSOURCE camera (DMK37BUX273) mounted on a stereomicroscope (LEICA M165FC). The worm was constantly kept in the center of the view field with a motorized microscope stage (OptiScanTM ES111 ...
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bioRxiv - Plant Biology 2020Quote: ... and germlings was measured after 15 min of exposure to NCR044.1 (50 μL, 3 μM) using fluorescence confocal microscopy (Leica SP8-X) with an excitation and emission wavelength of 488 and 508-544 nm ...
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bioRxiv - Neuroscience 2022Quote: ... cryopreserved and cryosectioned organoids (12-15 μm sections) were imaged following nuclear staining using Hoechst using a Leica DMI6000B microscope (Leica Microsystems) with an air 4x objective driven by Las-AF software ...
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bioRxiv - Neuroscience 2021Quote: ... The mouse was left for 15 min and positioned on the microscope stage and the spinal cord was imaged using a confocal microscope (Leica SPE). Vessels were identified as those that contained WGA555 labelled endothelium ...
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bioRxiv - Neuroscience 2021Quote: ... Plano, Germany) mounted on aluminum stubs (Plano, Germany) and were sputter-coated with a 15 nm gold layer (ACD600, Leica, Austria). SEM images were acquired with a JEOL JSM-IT200 SEM (JEOL ...
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bioRxiv - Cancer Biology 2020Quote: ... applied at either 1:300 or 1:500 for 15-30 minutes prior to detection and color visualization using Refine DAB Polymer (DS9800, Leica Biosystems). Rabbit primary antibodies were incubated for either 15 or 30 minutes followed by detection with Refine DAB Polymer ...
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bioRxiv - Microbiology 2020Quote: ... The capability to form spores was observed after starvation for 15 days using phase-contrast microscopy (Leica DM R, magnification 1000x). A differential staining for endospores and vegetative cells was performed using malachite green and safranine ...
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bioRxiv - Neuroscience 2023Quote: ... Brains were cryoprotected by incubating >24h in 15% sucrose in PBS followed by 30% sucrose before being frozen and sectioned at 30μm with a cryostat (Leica CM 1950). For stains using primary antibodies raised in mice ...
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bioRxiv - Neuroscience 2022Quote: ... samples on SEM stubs were coated with a 10 or 15 nm platinum or gold layer using the sputter coating machine EM ACE600 (Leica Microsystems) at 30 mA current to be finally placed into the Crossbeam 540 FIB-SEM (Carl Zeiss Microscopy GmbH ...
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bioRxiv - Immunology 2022Quote: ... the tissue slides were heat pre-treated using a ER1 (pH6) target retrieval buffer for 15 minutes at 97°C (AR9961, Leica Biosystems). The slides were then stained for multiplex immunofluorescence using the BOND RX Opal multiplex procedure (v7.0.3.216 RX ...
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bioRxiv - Cell Biology 2022Quote: ... using the RNAscope™ Multiplex Fluorescent V2 Assay (Cat. No. 323270) with tissue pretreatment of 15 minutes at 95°C using Bond Epitope Retrieval Solution 2 (Leica Biosystems), 15 minutes of Protease III (ACD ...
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bioRxiv - Bioengineering 2023Quote: ... the tissue was placed in a cryostat chamber at -20°C and sliced into 15 µm thick sections (CM1850, Leica microsystems). The FBR to the polymer coatings was assessed by staining with haematoxylin and eosin (H&E ...
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bioRxiv - Microbiology 2023Quote: ... The grid was blotted at once for 15 s from the back side and plunged into liquid ethane using EM GP2 (Leica Microsystems) at 18 °C and 90% humidity chamber setting ...
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bioRxiv - Neuroscience 2024Quote: Mice were sacrificed by cervical dislocation and sagittal slices (250-400 μm) were prepared with an angle of 10-15° to maintain rostrocaudal striatonigral projections 82 using a vibrating microtome (Leica VT1200). Slice preparation was performed in ice-cold sucrose-based artificial cerebrospinal fluid (ASCF ...
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bioRxiv - Cell Biology 2020Quote: Imaging of the mouse and human sections was carried with the DM5500 fluorescent microscope (Leica Microsystems). Images were captured with the Leica application suite X version ...
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bioRxiv - Genomics 2021Quote: Fixed human brain tissue (prefrontal cortex, BA10) was sectioned at 50 m using a vibratome (Leica). The sections were boiled in IHC Antigen Retrieval Solution (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... Attached RONAs and human cortical assemblies were sectioned (25 μm) by cryostat (CM3050; Leica, Nussloch, Germany) and collected on SuperFrost Plus glass slides (Roth ...
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bioRxiv - Neuroscience 2020Quote: ... containing dorsal hippocampus (from 3, 6, 9, 12, 15, 19, and 24 month-old) were cut from PFA-fixed hemispheres (Leica vibratome VT1000P) and stored in 0.1M phosphate-buffered saline (PBS ...
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bioRxiv - Developmental Biology 2019Quote: ... and cryosectioned at 20 μm thickness onto either Superfrost Plus glass slides (Fisher 12-550-15) (for staining optimization) or PEN membrane slides (Fisher/Leica No. 11505158) (for LCM) ...
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bioRxiv - Molecular Biology 2023Quote: ... Slides were baked at 60 °C for 15 min and deparaffinized with the Leica Bond Dewax solution (Leica Biosystems, Deer Park, IL), followed by heat-based antigen retrieval using Bond Epitope Retrieval Solution 1 (Leica Biosystems ...
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bioRxiv - Neuroscience 2020Quote: ... Fixed mouse brains and monkey or human brain samples were sectioned with a cryostat (model CM3050S, Leica) or a vibratome (Leica ...
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bioRxiv - Cell Biology 2020Quote: Western blot analysis for human iPSC–derived muscles was performed using antibodies to Dystrophin (NCL-DYS1, Leica), gamma-dystroglycan DAG1 (SC-165997 ...
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bioRxiv - Neuroscience 2022Quote: Paraffinized human optic nerves were cut in 5 μm sections using the microtome RM2155 (Leica, Wetzlar, Germany) and placed on 1 mm thick microscope slides (Marienfeld ...
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bioRxiv - Immunology 2023Quote: Platelet interactions with human monocytes were imaged in a Leica TCS SP5 SMD confocal system (Leica Microsystem). Cells were seeded in microslide 8-Well IBIDI chamber ...
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bioRxiv - Plant Biology 2020Quote: ... Dried samples were sputter-coated with argon–platinum plasma at a distance of 6–7 cm and 45 mA intensity for 15 sec in a sputtering chamber (Leica Microsystems EM MED020). Scanning electron micrographs were acquired using an AURIGA compact FIB-SEM (Zeiss ...
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bioRxiv - Pathology 2022Quote: ... Heat-induced epitope retrieval (HIER) was conducted by heating slides to 95°C for 15 minutes in EDTA-based ER2 buffer (Leica Biosystems, Richmond, IL). Slides were treated in protease (Advanced Cell Diagnostics ...
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bioRxiv - Pathology 2021Quote: ... Heat-induced epitope retrieval was conducted by heating slides to 95°C for 15 minutes in EDTA-based ER2 buffer (Leica Biosystems, Richmond, IL). The SARS-CoV-2 probe (cat ...
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bioRxiv - Neuroscience 2021Quote: ... Brains were extracted and snap-frozen on dry ice and sectioned at -20°C in 15 µm-thick coronal slices using a cryostat (Leica CM 1860 UV) and mounted onto Colorfrost Plus glass slides (Fisher 1255017) ...
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bioRxiv - Neuroscience 2023Quote: ... The brainstem areas containing the DVC and BAT sections were put in 15% followed by 30% sucrose until saturated and frozen in a cryo-embedding medium (Leica Biosystems, Nussloch, Germany). Brain sections were cut at 30 µm and BAT sections were cut at 5 µm using a cryostat (Leica Biosystems ...
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Critical contribution of mitochondria in the development of cardiomyopathy linked to desmin mutationbioRxiv - Pathology 2023Quote: ... At least 15 desmin-positive cardiomyocytes were imaged from each experiment with an inverted confocal microscope (Leica TCS SP5 AOBS, IBPS imaging platform). All images were acquired with a Z-step of 0.5 µm (image size 512x512 ...
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bioRxiv - Neuroscience 2022Quote: Human and mouse brain tissue was sectioned (30 μm-thick sections) using a SM2010 R sliding microtome (Leica) equipped with a BFS-3MP freezing stage and cooling unit (Physitemp ...
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bioRxiv - Cancer Biology 2021Quote: ... Sections from a series of 17 tumour samples from the validation cohort were stained for CD8 (mouse monoclonal 4B11, Leica Biosystems PA0183, used as supplied for 15 minutes at room temperature. HIER was performed on-board using Leica ER2 solution (high pH) for 20 minutes) ...
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bioRxiv - Microbiology 2020Quote: ... and 15 snapshots were taken per experiment in both the DIC and DiD channels with a Leica Biosystems 6000B instrument (Leica Biosystems, Amsterdam, The Netherlands). All snapshots were analyzed for total area of fluorescent spots quantified using the ParticleAnalzer plugin of ImageJ ...
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bioRxiv - Bioengineering 2022Quote: ... Samples were washed an additional 3 times in 100% ethanol for 15 minutes each and dried using a Leica EM CPD030 critical point dryer (Leica Microsystems, Buffalo Grove, IL) with CO2 as the transitional medium ...
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bioRxiv - Developmental Biology 2022Quote: Images from mouse and human samples were acquired with a Leica TCS SP8 confocal laser scanning microscope (Leica Microsystems). All images are shown as maximum intensity projections of acquired z-stacks of about one cell thickness (6-10 µm) ...
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bioRxiv - Immunology 2022Quote: ... serial human kidney sections were stained with anti-CD3 (LN10) or anti-CD8 (4B11; both Leica Biosystems, Chicago, IL) antibodies ...
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bioRxiv - Cancer Biology 2023Quote: ... automated methods on the Leica Bond Max autostainer using a mouse monoclonal anti-human CD3 antibody (Leica, clone LN10). Whole slide images of IHC stained slides were digitally captured using an Olympus IX83 microscope at 10x magnification and fixed exposure times ...
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bioRxiv - Microbiology 2020Quote: ... The phage free control and phage infected culture slides were sampled at 15-30 minute intervals and observed on an inverted fluorescent microscope (Leica DMi8 with LasX Premium software). Images are an overlay of DIC ...
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bioRxiv - Cell Biology 2021Quote: ... INS-1E cells were incubated with HO-PI for 15 min (5 mg/ml) and observed using a confocal laser-scanning microscope (Leica SP5; Leica Microsystems, Buffalo Grove, IL, USA).
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bioRxiv - Cell Biology 2019Quote: ... Immunofluorescence analyses of grafted TA muscles were performed using antibodies specific for: human lamin A/C (mouse monoclonal IgG2b, 1:400, NCL-Lam-A/C, Novocastra, Leica Biosystems ...
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bioRxiv - Cell Biology 2021Quote: Fixed and stained human pancreatic tissue sections were examined using a commercially available DragonFly200 spinning disk system (Andor) on a DMi8 microscope (Leica) with Plan Apo 40× NA 1.3 and Plan Apo 63× NA 1.4 oil immersion objectives ...
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bioRxiv - Cancer Biology 2021Quote: All staining of human tissues was performed on a Leica Bond RX automated stainer (Leica Microsystems, Inc., Buffalo Grove, IL). To ensure lectin specificity ...
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bioRxiv - Bioengineering 2022Quote: ... Bio SB) or anti-human CD163 (clone EP324, Bio SB) followed by an HRP-conjugated anti-mouse secondary antibody (Leica) and revealed with the peroxidase substrate 3 ...
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bioRxiv - Neuroscience 2023Quote: ... The entire human DRG section was imaged and merged using Leica Application Suite X (LAS-X, v.3.7, Leica Microsystems). Acquisition setting were kept consistent throughout imaging across different experimental days ...