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Citations for Leica :
101 - 150 of 316 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... The paraffin blocks containing the polycarbonate membranes surmounted by the cell monolayer were cut with a Histocore Rotary microtome (Leica Biosystems). The sections were then deparaffinised ...
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bioRxiv - Neuroscience 2023Quote: ... The removed brain is immersed for 4 minutes in iced oxygenated sucrose solution and then placed on a cellulose nitrate membrane to separate and position the hemispheres in the vibratome (Leica VT1200s) to obtain 400 µm horizontal slices (speed of 0.1 mm/s) ...
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bioRxiv - Pathology 2023Quote: ... (Supplementary table 1) were cut into 10 μm-thick sections and mounted on Leica PET-membrane slides (76463-320, Leica Microsystems). Postnatal lung tissues were considered as “healthy” after microscopic/histological analysis by a pulmonary pathologist ...
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bioRxiv - Microbiology 2023Quote: ... sectioned them at 10 µm thickness using a microtome and mounted the sections on thermoexitable POL-membranes (No. 115005191; Leica, Germany). Next ...
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bioRxiv - Cancer Biology 2023Quote: Fresh frozen tissues were placed in OCT and sections were cut at 7 μm thickness and mounted onto membrane slides (Leica, 11600288). The slides were fixed in ice-cold 70% EtOH for 5 min ...
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bioRxiv - Immunology 2023Quote: ... from Apoe-/-LysMCre and Apoe-/- LysMCreCnr1flox/flox male mice collected after 4 weeks WD were mounted on RNase-free membrane metal frame slides (POL FrameSlide, Leica Microsystems). Following deparaffinization under RNase-free conditions ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... harvested from the Inbred immunocompetent & NOD.SCID treatment study mice were stained for Cm major outer membrane protein (MOMP) using an automated staining platform (Leica Bond RX, Leica Biosystems). After deparaffinization and heat-induced epitope retrieval in citrate buffer at pH 6.0 ...
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bioRxiv - Neuroscience 2024Quote: ... The removed brain is immersed for 4 min in iced oxygenated sucrose solution and then placed on a cellulose nitrate membrane to separate and position the hemispheres in the vibratome (Leica VT1200s) to obtain 320 µm coronal slices (cutting speed of 0.12 mm/s) ...
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bioRxiv - Cancer Biology 2024Quote: ... A polymeric kit (Refine, Leica) was used as secondary reagent for detection ...
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bioRxiv - Cell Biology 2021Quote: ... HMC-1 cells were loaded into membrane carriers and were high-pressure frozen in a Leica EM PACT1 (Leica microsystems, Wetzlar, Germany). T ...
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bioRxiv - Molecular Biology 2023Quote: ... Tissue was stored in −80 °C and sectioned from an OCT block at 12 µm thickness and mounted on Leica PPS-membrane Laser Microdissection (LMD) slides (Leica, Cat# 11505268). A rapid stain protocol23 prepared slides for LMD ...
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bioRxiv - Microbiology 2023Quote: ... The dehydrated hNEC transwell membrane was then separated using a surgical blade and incubated in 65 °C paraffin (Leica EM-400 3801320) for 30 mins ...
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bioRxiv - Bioengineering 2023Quote: ... 10 images from different regions of the membrane were acquired with a fluorescence microscopy setup (Leica DM 2000 LED fitted with a Leica DFC450c camera).
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bioRxiv - Evolutionary Biology 2024Quote: ... 14-μm sections were prepared from the resulting blocks on a cryomicrotome (Microm HM 500 OM) and placed onto polyethylene napthalate membrane slides (Leica Microsystems, 11505158) which had been exposed to UV irradiation in a biosafety cabinet (Baker SterilGARD ...
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bioRxiv - Immunology 2022Quote: ... using the RNAScope 2.5 LS multiplex fluorescent assay (ACD, Bio-Techne) on the automated Leica BOND RX system (Leica). Cryosections were 10 µm-thick and placed on superfrost plus slides ...
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bioRxiv - Cell Biology 2021Quote: ... Ten micron-thick cryosections cut on to superfrost plus slides were processed using the RNAScope 2.5 LS multiplex fluorescent assay (ACD, Bio-Techne) on the Leica BOND RX system (Leica). Fresh frozen lung sections were fixed for 90 minutes with chilled 4% paraformaldehyde ...
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bioRxiv - Cell Biology 2022Quote: ... Assay was performed under CO2 and temperature-control within the live-cell incubation chamber of the Thunder microscope (Leica). Mitochondrial iron translocation (MIT ...
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bioRxiv - Cancer Biology 2021Quote: ... followed by a proprietary automatic staining assay developed for the BOND RX automated stainer (Leica Biosystems Division of Leica Microsystems Inc ...
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bioRxiv - Immunology 2022Quote: ... µm thick sections were cut on to SuperFrost® Plus slides and processed using the RNAscope 2.5 LS multiplex fluorescent assay (ACD, Bio-Techne) on the Leica BOND RX system (Leica). FFPE slides were baked and dewaxed (BOND protocol) ...
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bioRxiv - Neuroscience 2023Quote: ... This IF assay was carried out on the Bond Rx fully automated slide staining system (Leica Microsystems, Wetzler Germany) using the Bond Research Detection kit (DS9455) ...
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bioRxiv - Cancer Biology 2022Quote: Microscopic images from in vitro and in vivo assays were analyzed with Leica Application Suite X software (Leica Microsystems) and free ImageJ Fiji software (NIH Image) ...
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bioRxiv - Cell Biology 2024Quote: ... Selected regions were analyzed using Measure Stained Area Assay of Slidepath Tissue Image Analysis 2.0 software (Leica Microsystems, Germany). A quantitative analysis of cartilage ECM staining areas was performed using FIJI image calculator.
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bioRxiv - Cell Biology 2020Quote: ... were transferred to 100 μm deep membrane carriers and high pressure frozen with a Leica EM PACT2 or Leica EM ICE freezers (Leica Microsystems, Wentzler, Germany). All samples were processed by freeze substitution in a Leica AFS2 temperature-controlling machine (Leica Microsystems ...
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bioRxiv - Microbiology 2022Quote: Cell pellets in cryoprotectant solution were loaded onto membrane carriers (Mager Scientific, Dexter, MI) and cryo-fixed using the EM PACT2 high pressure freezer (Leica, Buffalo Grove, IL). Cryo-fixed cells were processed by freeze substitution using an AFS2 automated freeze substitution unit (Leica) ...
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bioRxiv - Neuroscience 2023Quote: ... The tissue used for cell-level gene expression studies was cryostat sectioned into 12 μm thick sections on PEN membrane glass slides (Leica Microsystems, Wetzlar, Germany).
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... harvested from the Inbred immunocompetent & NOD.SCID treatment study mice were stained for Cm major outer membrane protein (MOMP) using an automated staining platform (Leica Bond RX, Leica Biosystems). After deparaffinization and heat-induced epitope retrieval in citrate buffer at pH 6.0 ...
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bioRxiv - Microbiology 2024Quote: ... with the BPRD kit (DS9800, Leica) reagents ...
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bioRxiv - Cell Biology 2020Quote: Imaging data for the under agarose assay 1 were collected using a Leica DMI-4000B inverted microscope (Leica Microsystems Inc.) mounted on a TMC isolation platform (Technical Manufacturing Corporation ...
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bioRxiv - Cell Biology 2020Quote: ... The microtubule assays in MDA-MB-231 cells were conducted at the Einstein Analytical Imaging Facility on a SP5 AOBS (Leica) confocal microscope with a 63x (1.4 NA ...
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bioRxiv - Neuroscience 2020Quote: ... CB and N2a cells in the Huntingtin cytotoxicity assay were imaged with the DMi8 inverted microscope with thunder imaging platform (Leica) with 20x objective and incubation (33°C for CB cells ...
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bioRxiv - Developmental Biology 2021Quote: Pictures from co-localization assays and immunolabelling of brain slices were performed with a confocal laser scanning TCS SP5 (Leica) and the software Leica Application Suite Advanced Fluorescent lite (LAS Af lite ...
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bioRxiv - Cancer Biology 2024Quote: ... the SignalStar™ Multiplex Immunohistochemistry Assay (Cell Signalling Technologies) for Use on BOND RX Fully Automated Research Stainer (Leica Biosystems) was followed as per the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2022Quote: ... and 3-8 randomly chosen fields per view per Matrigel assay were acquired using confocal Z-stack imaging (TCS-SP8; Leica). Attached pericytes were counted by using the LASX Software.
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bioRxiv - Cell Biology 2023Quote: ... Sections were imaged with a THUNDER 3D Assay inverted microscope equipped with a Leica K3C Color camera (Leica Microsystems, Germany). High-magnification images were acquired within 200 μm of the optic nerve head (on both sides) ...
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bioRxiv - Neuroscience 2024Quote: ... Slices were imaged within 1 week of performing the assay using the 0.75 NA 20X objective on a Leica TCS SP8 laser scanning confocal microscope (Leica Microsystems) at 2 µm depth intervals ...
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bioRxiv - Microbiology 2024Quote: ... Both plate and time lapse motility assays were images with a Leica SD9 dissecting microscope equipped with a Leica Flexcam C3 camera controlled by Leica LAS X software ...
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bioRxiv - Neuroscience 2024Quote: ... LS 4-Plex Ancillary Kit and Multiplex Reagent Kit on a robotic staining system (Leica BOND-III). RNAscope probes were Hcn1 (ACD ...
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bioRxiv - Molecular Biology 2023Quote: ... The membranes were then cut out and mounted on a glass slide for imaging with a DMi8 inverted fluorescent microscope (Leica Microsystems, Buffalo Grove, IL).
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bioRxiv - Biochemistry 2024Quote: ... onto glass slides for generating diagnostic hematoxylin and eosin (H&E) sections for pathology review or sectioned (10 µm) onto polyethylene naphthalate (PEN) membrane slides (Leica Microsystems, Inc., Deerfield, IL) to harvest tissue by LMD for nucleic acid isolations ...
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bioRxiv - Cancer Biology 2024Quote: ... Cat# 415798-C2) and the RNAscope LS Multiplex Fluorescent Assay (ACD, Cat# 322800) using the Bond RX auto-stainer (Leica Biosystems) with a tissue pretreatment of 15 minutes at 95°C with Bond Epitope Retrieval Solution 2 (Leica Biosystems) ...
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bioRxiv - Cell Biology 2022Quote: ... using the RNAscope™ Multiplex Fluorescent V2 Assay (Cat. No. 323270) with tissue pretreatment of 15 minutes at 95°C using Bond Epitope Retrieval Solution 2 (Leica Biosystems), 15 minutes of Protease III (ACD ...
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bioRxiv - Genetics 2023Quote: Fluorescence images (dye assay) and time-lapse movies (IFT movies) were captured using an epi-fluorescence upright microscope (Leica DM6 B) (3 frames per second ...
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bioRxiv - Microbiology 2024Quote: ... ORF75 RNA expression was detected in these biopsies by staining 5 um FFPE tissue sections with RNAscope™ 2.5 LS Probes V-HHV8-ORF75 (ACD, Cat# 562058) using the RNAscope LS Multiplex Fluorescent Assay (ACD, Cat# 322800) with a Bond RX auto-stainer (Leica Biosystems) Multiplex Fluorescent Assay (ACD ...
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bioRxiv - Neuroscience 2023Quote: ... At day EP14 slices were cut out along with the membrane and placed into the perfusion chamber mounted on a spinning disc microscope (Leica DMI 6000B (Leica Microsystems, Inc., Buffalo Grove, IL), with an Andor CSU W1 spinning disc unit ...
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bioRxiv - Cancer Biology 2023Quote: ... or Bond proteolytic enzyme kit (AR9551, Leica). Thereafter ...
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bioRxiv - Physiology 2023Quote: ... or BondTM proteolytic enzyme kit (AR9551, Leica). Sections were then incubated with the indicated antibodies in BondTM primary antibody diluent (AR9352 ...
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bioRxiv - Cancer Biology 2024Quote: ... or BondTM proteolytic enzyme kit (AR9551, Leica) were used for antigen retrieval (Supplementary Table 15) ...
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bioRxiv - Microbiology 2022Quote: ... Cat# 897938-C3) using the RNAscope® LS Multiplex Fluorescent Assay (ACD, Cat# 322800) with a Bond RX auto-stainer (Leica Biosystems) with a tissue pretreatment of 15 minutes at 90°C with Bond Epitope Retrieval Solution 2 (Leica Biosystems) ...
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bioRxiv - Cancer Biology 2024Quote: The assay consisted of the following antibodies and corresponding dilution factor: CK AE1/AE3 (Leica Biosystems, ref. AE1/AE3-601-L-CE) was applied at 1:250 for 28 minutes at 37°C ...
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bioRxiv - Plant Biology 2021Quote: ... map70-5-c1 plants were crossed with the W131Y cell membrane marker line and homozygous F3 plants imaged and staged from stage I until stage IV (Leica SP8-MP DIVE 960 nm excitation). To determine total root and LRDZ length of ELTPpro>>PHS1ΔP in the UBQ10pro::EYFP:NPSN12 and map70-5 mutants ...