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Citations for Leica :
101 - 150 of 1523 citations for 7 AMINO 2 TERT BUTOXYCARBONYL 1 2 3 4 TETRAHYDROISOQUINOLINE 3 CARBOXYLIC ACID since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2020Quote: ... at least 3 transverse slices cut with a vibratome (VT1000S, Leica) at different anterior-posterior levels representing anterior ...
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bioRxiv - Neuroscience 2019Quote: ... The hippocampal regions were loaded into 3 mm aluminium planchettes (Leica Cat# 1677141 for type A and 1677142 for type B ...
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bioRxiv - Neuroscience 2019Quote: ... for 3 min and uranyl acetate (Leica Ultrostain I, Leica Microsystems) for 10 min using an automatic contrasting system (Leica EM AC20 ...
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bioRxiv - Neuroscience 2019Quote: ... for 3 min and uranyl acetate (Leica Ultrostain I, Leica Microsystems) for 10 min using an automatic contrasting system (Leica EM AC20 ...
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bioRxiv - Neuroscience 2023Quote: ... and sections (3 µm) were cut using a microtome (LEICA RM2255). The sections were deparaffinized by bathing twice in xylene (for 5 min each ...
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bioRxiv - Microbiology 2023Quote: ... Tissues were sectioned at 3 μm using a microtome (Leica, Germany) and the sections were stained with hematoxylin–eosin (HE ...
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bioRxiv - Systems Biology 2023Quote: ... we collected 3 10 µm sections using a Microtome (Leica Byostems). We then extracted RNA using Qiagen RNAse FFPE kit according to manufacturer instructions and evaluated the percentage of total RNA fragments longer than 200 nucleotides (DV200 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The animals were given cold shock for 2 minutes at -4°C and then photographed along with a reference scale under a stereo zoom microscope (Leica DFC 290 ...
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bioRxiv - Synthetic Biology 2023Quote: ... Transgenic fish were screened at 2-4 dpf for CFP expression in the eyes using a CFP filter (Leica, 10447409) on a fluorescence stereomicroscope (Leica ...
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bioRxiv - Neuroscience 2020Quote: ... 3× 1 min then washed with 70% alcohol 3x followed by Bond Wash solution (AR9590, Leica Biosystems, Wetzler, Germany) 3x ...
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bioRxiv - Neuroscience 2020Quote: ... Brains were isolated and immersed in 20% sucrose for 1 day and 30% sucrose for 2 days at 4°C before being sectioned at 40 μm thicknesses on a cryostat microtome (Leica, CM3050s). Brain slices were collected and blocked with blocking solution containing 2% Normal goat serum (v/v) ...
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bioRxiv - Neuroscience 2021Quote: ... Brains were harvested and stored in the same solution at 4 °C for 2 h before being sliced into 150 μm-thick slices on a vibratome (Leica VT1000S). The slices were stored in 0.1 M glycine and 0.01% sodium azide in 1× PBS at 4 °C until use.
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bioRxiv - Microbiology 2020Quote: Total cell counts were determined by staining with 4’,6-diamidino-2-phenylindole (DAPI) and visualizing on a Leica DMRX epifluorescence microscope (Leica, Germany). The number of FLA-YM stained cells were determined by enumerating cells which had a positive DAPI and FLA-YM signal (excitation at 405 nm and 488 nm wavelengths ...
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bioRxiv - Neuroscience 2022Quote: ... post-fixed in the 4% PFA overnight, then embedded in 2% low-melting agarose (Lanza, #50115) and vibratome (VT1200S, Leica, Germany) sectioned at 100μm ...
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bioRxiv - Developmental Biology 2024Quote: ... the brain was incubated in 4% PFA at 37°C for 2 weeks and divided into 100-μm thick sections by a vibratome (Leica VT200S). Serial brain slices were immediately collected ...
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bioRxiv - Biochemistry 2020Quote: ... Confocal measurements were performed using a TCS SP8 STED 3× (Leica Microsystems) equipped with an HC PL APO CS2 100× objective (NA 1.4 ...
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bioRxiv - Neuroscience 2020Quote: ... slices were mounted in 3% agarose and sliced using a vibratome (Leica VT1200S ...
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bioRxiv - Cell Biology 2021Quote: ... Then 3-µm sections were made on a rotary microtome (RM2235, Leica). Slides were immediately stained for 1 minute in Mayer’s hematoxylin solution (Electron Microscopy Sciences) ...
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bioRxiv - Microbiology 2021Quote: ... sections cut to 3 µm thickness using a Leica microtome (Leica, Germany) and then stained using Quick Dip Stain Kit (Modified Giemsa Stain ...
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bioRxiv - Cell Biology 2021Quote: ... and infiltrated with wax through 3 Paraplast Plus paraffin baths (39602004; Leica). Samples were embedded in Paraplast Plus using paraffin-filled stainless steel base molds and a Thermo-Shandon Histocenter 2 Embedding Workstation (6400012D ...
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bioRxiv - Plant Biology 2024Quote: ... a flat specimen carrier of 3 mm diameter (Leica Microsystems, Vienna, Austria) was first filled with a drop of 20% BSA (bovine serum albumin ...
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bioRxiv - Molecular Biology 2024Quote: ... sections cut to 3 µm thickness using a Leica microtome (Leica, Germany), then samples were transferred to the Piper Hanley laboratory ...
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bioRxiv - Neuroscience 2023Quote: ... until the brains sank in the solution (1-3 days) and at which point 35um thick hypothalamic sections were obtained using a cryostat (Leica). Sections were first incubated for 1 hour in blocking buffer (1X PBS with 2% BSA and 0.1% tween-20) ...
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bioRxiv - Neuroscience 2022Quote: ... in Epitope Retrieval Solution 2 (Leica, AR9640) for 20 minutes at 100°C ...
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bioRxiv - Cell Biology 2024Quote: ... Epitope Retrieval Solution 2 (Leica Cat#AR9640), Retrieval Solution Low pH (Agilent Cat#GV80511-2) ...
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bioRxiv - Neuroscience 2024Quote: ... gel blocks were cut into cuboid shapes (2×2×1.3 mm) with a vibratome (Leica VT1000S, Leica Biosystems GmbH). Scaffolds were then precisely inspected under a light microscope (Olympus BX53 ...
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bioRxiv - Neuroscience 2024Quote: ... gel blocks were cut into cuboid shapes (2×2×1.3 mm) with a vibratome (Leica VT1000S, Leica Biosystems GmbH). Scaffolds were then precisely inspected under a light microscope (Olympus BX53 ...
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bioRxiv - Cell Biology 2022Quote: ... Dried samples were then rotary shadowed with 2 nm of platinum and 4-6 nm of carbon using the ACE600 (Leica Microsystems, Germany). Areas of interest from the resulting platinum replicas were separated from glass with 5% hydrofluoric acid and deposited on carbon-coated EM grids (200 mesh formvar/carbon ...
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bioRxiv - Cell Biology 2022Quote: ... Dried samples were then rotary shadowed with 2 nm of platinum (sputtering) and 4-6 nm of carbon (carbon thread evaporation) using an ACE600 metal coater (Leica Microsystems, Germany). The resultant platinum replica was floated off the glass with hydrofluoric acid (5%) ...
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bioRxiv - Microbiology 2024Quote: ... containing 4‘,6-diamidino-2-phenylindole (DAPI) and imaged as midsections by confocal laser scanning microscopy (Leica SP8: Leica Microsystems, Wetzlar, Germany). To prevent cross talk between the channels for the different fluorochromes ...
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bioRxiv - Cell Biology 2023Quote: ... Dried samples were then rotary shadowed with 2 nm of platinum (sputtering) and 4-6 nm of carbon (carbon thread evaporation) using an ACE600 metal coater (Leica Microsystems, Germany). The resultant platinum replica was floated off the glass with hydrofluoric acid (5%) ...
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bioRxiv - Cell Biology 2019Quote: Images of ZEB1 transgenic ZEB1+/+ and ZEB1+/- cell cultures at day 1 (sub-confluent) and at day 3 post-seeding (confluent) were acquired using the Leica DMIL LED inverted microscope (Leica Microsystems) and the N PLAN L 20x/0.35 PH1 objective ...
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bioRxiv - Neuroscience 2020Quote: ... Mice were anesthetized with a mixture of oxygen (1 L/min) and isoflurane 3% (Baxter AG, Vienna, Austria) and placed in a stereotactic frame (Angle One; Leica, Germany). The skin was shaved ...
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bioRxiv - Neuroscience 2022Quote: ... Brains were post-fixed for 1–3 days and sectioned coronally (50–100 μm in thickness) using a vibratome (Leica; VT1000S). Brain sections were mounted on superfrost slides and coverslipped with ProLong antifade reagent with DAPI (Molecular Probes) ...
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bioRxiv - Cell Biology 2020Quote: STED images were taken on a TCS SP8 STED 3 × microscope (Leica Microsystems) on a DMI8 stand using a 100 × 1.4 NA oil HCS2 PL APO objective and a pulsed supercontinuum light source (white light laser) ...
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bioRxiv - Pathology 2021Quote: ... 3 μm sections were cut using a rotatory microtome (Leica RM2135, Nussloch, Germany). Nine slides from each organ were cut.
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Sections of 3 μm were cut and mounted on adhesive slides (Leica Xtra) and rehydrated through a series of xylene and alcohol washes after which slides were rinsed in distilled water ...
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bioRxiv - Cell Biology 2021Quote: ... 3 µm plastic sections were cut with a microtome (RM 223, Leica, Germany) and stained with hematoxylin and eosin according to the manufacturer’s instruction (UI0402 ...
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bioRxiv - Developmental Biology 2022Quote: ... embryos were paraffin-embedded and serially sectioned at 3-5 µm (Leica microtome), before attachment to poly-L-lysine-coated glass slides (Sigma ...
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bioRxiv - Developmental Biology 2023Quote: ... Tissue sections (3 μm thick) were prepared with the RM2125 RTS microtome (Leica) and were mounted on Polysine slides (Thermo Fisher Scientific ...
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bioRxiv - Synthetic Biology 2022Quote: ... #1 prior to plunging into liquid ethane (−182°C) in a Leica EM GP 2 (Leica) vitrification robot (automated blot pressure ...
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bioRxiv - Plant Biology 2021Quote: ... The roots were washed with 1/2 MS media and imaged by fluorescence microscopy (Leica, M205FA). The quantitative measurement of ROS staining intensity was performed as average pixel intensities using ImageJ software [28].
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bioRxiv - Neuroscience 2022Quote: ... at - 45°C for 2 days and UV-polymerized for 1 day in EM AFS2 (Leica). The aBA-containing polymerized blocks were further sliced into 100 nm thickness by an ultra-microtome (Leica) ...
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bioRxiv - Immunology 2022Quote: ... Approximately 2 μl of each sample was processed by HPF using a Leica EM PACT 2 (Leica, Milton Keynes, UK). After HPF ...
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bioRxiv - Biochemistry 2023Quote: ... Grids were glow discharged in air for 2 min and sample (2 μL) applied in an EM GP2 plunge freezer (Leica) at 4°C and 90% humidity ...
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bioRxiv - Developmental Biology 2021Quote: ... The tissues were merged in 10% and 30% sucrose-PBS solutions at RT (2 h each solution) followed by 1:1 solution 30% sucrose:tissue-freezing medium (Leica 14020108926). Afterwards ...
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bioRxiv - Cancer Biology 2020Quote: ... Bcl-2 (clone 3.1 mouse monoclonal antibody, Leica) was used at a 1:100 dilution with epitope retrieval solution 2 pre-treatment for 20 min.
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bioRxiv - Cancer Biology 2023Quote: ... using BOND Epitope Retrieval Solution 2 (Leica Biosystems). Detection was achieved using a Bond Polymer Refine Detection® DAB chromogen kit and counterstained with hematoxylin ...
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bioRxiv - Immunology 2023Quote: ... and BOND Epitope Retrieval Solution 2 (Leica AR9640) for MPO antibody ...
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bioRxiv - Pathology 2023Quote: ... speed 2 (Oxford) or 0.5 mm/s (Leica), and blade angle slightly below 0° (Oxford ...