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Citations for Leica :
101 - 150 of 1935 citations for 6 METHYL 2 OXO 4 THIOPHEN 2 YL 1 2 3 4 TETRAHYDRO PYRIMIDINE 5 CARBOXYLIC ACID ETHYL ESTER since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2019Quote: ... Then a bulk of muscle was washed 2 to 3 times in cold PBS and fixed on the specimen holder of VT1200S vibrating blade microtome (Leica Biosystems, US). The buffer tray of microtome was ice bathed and filled with cold PBS while slicing ...
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bioRxiv - Cancer Biology 2019Quote: ... homogeneous and mixed spheroids were generated and imaged repeatedly over time (2-3 times per week) using brightfield microscopy (4x objective; 0.3 NA, Leica DM IRBE, Hamamatsu). Spheroid volumes were calculated using SpheroidSizer58.
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bioRxiv - Cell Biology 2020Quote: ... Dried samples were then rotary-shadowed with 2 nm of platinum and 5-8 nm of carbon using an ACE600 high vacuum metal coater (Leica Microsystems). Platinum replicas were floated off the glass by 5% hydrofluoric acid ...
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bioRxiv - Cell Biology 2020Quote: ... cells were loaded with fura-2/AM in the dark (5 μM; 30 min; RT; S1) and imaged with an upright microscope (Leica DMI6000FS, Leica Microsystems) equipped for ratiometric live-cell imaging with a 150W xenon arc lamp ...
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bioRxiv - Cell Biology 2019Quote: ... Dried samples were then rotary-shadowed with 2 nm of platinum and 5-8 nm of carbon using an ACE600 high vacuum metal coater (Leica Microsystems). The resultant platinum replica was floated off the glass with hydrofluoric acid (5%) ...
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bioRxiv - Neuroscience 2024Quote: Mice were anesthetized using vaporized isoflurane (5% for induction, 1.5% for maintenance) and placed in a stereotactic frame (Leica, Angle 2). Opthalmic ointment was applied and rectal temperature and respiration rate were monitored throughout surgery ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... About 3-4 µm sections were prepared using an automated microtome (RM2255, Leica Biosystems, Germany) and the sections were stained with hematoxylin and eosin (H&E ...
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bioRxiv - Plant Biology 2022Quote: ... seedlings were mounted on a glass slide in 1/2 MS medium and imaged on SP8 (Leica). YFP was excited by the 514-nm line of the argon laser and detected between 525 and 580 nm ...
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bioRxiv - Neuroscience 2019Quote: ... Pictures were taken with a Leica microscope (Axioscop 2, Leica GmbH).
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bioRxiv - Neuroscience 2021Quote: ... Slices were visualized on a resonant scanning 2-photon system (Leica) and Z-stack images were taken ...
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bioRxiv - Cancer Biology 2020Quote: ... followed by pretreatment with Epitope Retrieval Solution 2 (AR9649, Leica Biosystems) for 20 minutes and then blocking buffer for 10-20 minutes ...
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bioRxiv - Cancer Biology 2019Quote: ... Panel 2 consisted of primary antibodies against CD3 (clone LN10, Leica), PD-1 (clone NAT105 ...
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bioRxiv - Immunology 2023Quote: ... Fura-2-AM was excited using a monochromatic light source (Leica) coupled into an inverted Leica DMI4000B microscope that was equipped with a 20x objective (HCX PL Fluotar 20x ...
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bioRxiv - Cancer Biology 2023Quote: ... anti-BCL2 (Clone: BCL-2/100/D5; RTU; Leica Biosystems; #PA0117), anti-BCL6 (Clone:LN22 ...
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bioRxiv - Molecular Biology 2023Quote: ... sputter-coated with 2 nm carbon using ACE600 sputter coater (Leica).
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bioRxiv - Developmental Biology 2021Quote: ... The tissues were merged in 10% and 30% sucrose-PBS solutions at RT (2 h each solution) followed by 1:1 solution 30% sucrose:tissue-freezing medium (Leica 14020108926). Afterwards ...
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bioRxiv - Neuroscience 2020Quote: ... Representative images were taken from the cortical layer 2-3 with Nikon-DS Fi3 camera attached to a Leica DM 2000 Led light microscope (Leica Microsystems GmbH, Germany).
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bioRxiv - Neuroscience 2020Quote: ... The brain was then embedded in 2 to 3% agar and 100 μm thick sections were cut using a microtome (Leica VT1000 S vibratome). Slices were mounted using a Vectashield HardSet mounting medium containing DAPI (H-1500-10 ...
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bioRxiv - Neuroscience 2022Quote: ... We prepared horizontal brain slices containing the somatosensory S2 cortex and the corresponding corticostriatal projection field in the dorsal striatum from mice (2-3-months old) using a vibrating blade microtome (VT1200S, Leica Micosystems, Nussloch, Germany). Brains were sliced in a 5% CO2/95% O2-bubbled ...
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bioRxiv - Cancer Biology 2023Quote: ... Slides were rinsed in tap water and dipped 3-5 times in 0.5% Acid Alcohol (Leica Biosystems 3803651), followed by rinsing in tap water and bluing with Scott’s Tap Water (Electron Microscopy Sciences 2607007 ...
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bioRxiv - Microbiology 2020Quote: ... samples were de-stained with 50% Lactophenol for 1–2 hours before observation under a light microscope (Leica Microscope ...
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bioRxiv - Immunology 2021Quote: ... After fixation organs were stored in PBS till paraffin embedment and cut of serial sections on a microtome (2-3 µm; Leica RM 2035; Leica Instruments GmbH, Nuβloch, Germany). Sections were stained by hematoxylin and eosin (HE ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The growth culture medium was changed every 2-3 days and cell morphology was regularly checked by visual inspection with phase-contrast microscopy (Leica DMIC, Leica Microsystem GMbH, Wetzlar, Germany). When the degree of confluence reaches approximately 80 % (every 4-6 days) ...
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bioRxiv - Cell Biology 2021Quote: ... Cell nucleus was defined and exposed to 405 nm laser light for 4-5 times 200ms bursts at ∼5% 405 nm laser power (TCS SP8 MP, Leica). After photoconversion of Dendra2-RUVBL2 ...
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bioRxiv - Cancer Biology 2019Quote: ... the TMA slide was subjected to Epitope Retrieval Solution 2 (ER2, Leica) antigen retrieval and stained with a prediluted anti-Ki-67 antibody (Mouse ...
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bioRxiv - Zoology 2020Quote: ... We obtained 2 μm sections in rotating microtome (RM 2265, Leica, Switzerland), which were stained with hematoxylin and eosin.
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bioRxiv - Cell Biology 2021Quote: ... Samples were stained in 2% aqueous uranyl acetate and lead citrate (Leica) and imaged on a Hitachi HT7800 transmission electron microscope ...
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bioRxiv - Pathology 2020Quote: ... Epitope retrieval was performed using Epitope Retrieval solution 2 (Leica Biosystems, Germany) for 20 min ...
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bioRxiv - Immunology 2021Quote: ... Tubes were loaded into an AFS-2 freeze-substitution machine (Leica Microsystems) and processed at −90°C for 72 h ...
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bioRxiv - Pathology 2021Quote: ... and ddH2O (2 washes) with a Leica ST4020 Linear Stainer (Leica Biosystems). Tissues next underwent antigen retrieval by submerging slides in 3-in-1 Target Retrieval Solution (pH 9 ...
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bioRxiv - Microbiology 2023Quote: ... and 2% lead-citrate solution (Leica Ultrostain II, Leica Microsystems, Wetzlar, Germany) for 10 min each ...
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bioRxiv - Developmental Biology 2023Quote: Worms (young adults) were fixed by HPF with EMPACT-2 (Leica Microsystems) and then freeze substituted in anhydrous acetone containing 1% OsO4 ...
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bioRxiv - Plant Biology 2023Quote: ... Sections (2-µm thick) were cut using an UC7&2265 microtome (Leica). To visualize starch granules and protein bodies ...
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bioRxiv - Cell Biology 2023Quote: ... Tubes were loaded into an AFS-2 freeze-substitution machine (Leica Microsystems) and processed at -90°C for 72 h ...
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bioRxiv - Plant Biology 2024Quote: ... Sections (2-µm thick) were cut using a UC7&2265 microtome (Leica). To visualize starch granules and protein bodies ...
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bioRxiv - Neuroscience 2024Quote: ... All procedures were carried out using to Angle 2 software (Leica Biosystems) to set and locate coordinates ...
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bioRxiv - Cell Biology 2020Quote: ... cells were loaded with fura-2/AM in the dark (5 μM; 30 min; RT; S1) and imaged with an upright microscope (Leica DMI6000FS, Leica Microsystems) equipped for ratiometric live-cell imaging with a 150W xenon arc lamp ...
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bioRxiv - Cell Biology 2022Quote: ... and the cells were seeded at 2000 cells/ml (total volume 2 ml) before starting the experiment at 37ºC supplemented with 5% CO2 in a live cell chamber (Leica DMI6000B with PEKON stage). Cells that attached and spread during the first two hours were used to quantify the changes in traction stresses during adhesion ...
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bioRxiv - Bioengineering 2022Quote: ... The homogeneity of BMP-2 loading inside the film-coated 3D mini-scaffolds was assessed using a BMP-2 loading solution containing 5% of BMP-2Rhod and a fluorescence macroscope (Macrofluo Z16 Apo, Leica Microsystems, Wetzlar, Germany) with a 0.8X objective ...
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bioRxiv - Developmental Biology 2019Quote: ... Serial sections of 4–6 µm thickness were cut using a Leica RM2255 rotary microtome (Leica Microsystems GmbH, Germany) with disposable blades ...
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bioRxiv - Molecular Biology 2020Quote: ... the individual primordial oocyte was collected from 2-3 adjacent tissue sections to ensure that one full cell was included (Leica Microsystems, Buffalo Grove, IL, USA).
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bioRxiv - Plant Biology 2023Quote: Nodules were embedded in 4-5% agarose and sliced in 100um sections using a vibratome (Leica). Samples were immediately imaged on slides using a 20x air objective using the excitation and emission settings described above ...
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bioRxiv - Bioengineering 2022Quote: ... the laser scanning setup was chosen (Leica Sp8 SMD, see suppl. video 2): a small square ROI of ca ...
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bioRxiv - Plant Biology 2021Quote: ... Fluorescent images were observed under confocal laser scanning microscopy (TCS SP5 2; Leica).
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bioRxiv - Cell Biology 2021Quote: ... for cryosection preparations or in 2% agarose for sectioning with a vibratome (Leica). Sections were subsequently blocked using 1%BSA in TBS containing 0.05% Tween 20 (Sigma ...
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bioRxiv - Cell Biology 2022Quote: ... glo-2 mutants were phenotyped on a DM5500 wide-field fluorescence microscope (Leica) for the absence of auto-fluorescent and birefringent gut granules ...
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bioRxiv - Neuroscience 2021Quote: ... Sections of 2 μm thickness were sliced on an ultramicrotome (Leica EM UC6) at −80°C and placed onto gridded coverslips (type 1.5 H ...
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bioRxiv - Microbiology 2021Quote: ... and sputter coated with ∼2 nm iridium (EM ACE600; Leica, Buffalo Grove, IL). Imaging was done using a Hitachi SU8230 field emission instrument at 0.8 kV using the low-angle backscatter and secondary electron detectors.
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bioRxiv - Neuroscience 2020Quote: ... Paraffin sections (2 µm in thickness) were generated using a sliding microtome (Leica). Prior to immunoreactions ...
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bioRxiv - Cell Biology 2020Quote: FRET imaging experiments were performed using an epifluorescence inverted microscope (DMIRE-2, Leica) with a PlanApo 40x oil immersion objective ...