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Citations for Leica :
101 - 150 of 944 citations for 5 M TOLYL FURAN 2 CARBALDEHYDE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: Twenty-μm-thick coronal sections containing the MS and the CPU were prepared with a Leica SM 2000R freezing microtome (Leica Microsystems) and stored at -20 °C in 24-well tissue culture plates containing antifreeze solution [30% ethylene glycol ...
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bioRxiv - Neuroscience 2023Quote: ... The gelatin cube was sectioned coronally with 100 μm thickness (except for sagittal sections tilted 12 degrees away from the midline in Fig. 1) using a vibratome (Leica VT1200). Imaging was performed using upright confocal laser-scanning microscope (LSM880 ...
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bioRxiv - Neuroscience 2023Quote: ... Free floating brain sections were then prepared by sectioning these fixed brains into 40-μm-thick sections in the coronal plane with a Leica VT-1000 Vibrating microtome or Sliding Microtome SM-2000R (Leica Microsystems).
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bioRxiv - Neuroscience 2023Quote: ... the mice were perfused intracardially with heparinized 0.1 M PBS (10 U/ml of Heparin, Ratiopharm, N68542.03; 100-125 mmHg pressure using a Leica Perfusion One system) for 5 minutes to remove the blood ...
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bioRxiv - Neuroscience 2023Quote: ... was cryoprotected with 30% sucrose in PBS 0.1 M for 48 h and cut afterwards in 50-µm-thick coronal sections using a sliding microtome (LEICA SM2000R, Leica). Slices were collected in 6 subseries and stored at −20°C in a cryoprotective solution (30% glycerol ...
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bioRxiv - Neuroscience 2024Quote: Mice were sacrificed by cervical dislocation and sagittal slices (250-400 μm) were prepared with an angle of 10-15° to maintain rostrocaudal striatonigral projections 82 using a vibrating microtome (Leica VT1200). Slice preparation was performed in ice-cold sucrose-based artificial cerebrospinal fluid (ASCF ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were pelleted by centrifugation at ∼800 RCF for five minutes and high-pressure frozen in the 200-µm recess of an aluminum platelet (Engineering Office M. Wohlwend 241) using an HPM100 (Leica Microsystems). The samples were then freeze substituted in 0.1% uranyl acetate ...
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bioRxiv - Biophysics 2024Quote: ... Dissected egg chambers were high-pressure frozen in gold-coated copper Type A carriers (Engineering office M. Wohlwend) using the HPM010 (Wohlwend) or the EM Ice high-pressure freezing systems (Leica Microsystems). The egg chambers were added into the 100 µm or 200 µm cavity of the planchette and 20% Ficoll (70 kDa ...
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bioRxiv - Neuroscience 2022Quote: ... in Epitope Retrieval Solution 2 (Leica, AR9640) for 20 minutes at 100°C ...
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bioRxiv - Neuroscience 2023Quote: ... and 3% lead citrate (Ultrostain 2, Leica).
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bioRxiv - Cell Biology 2024Quote: ... Epitope Retrieval Solution 2 (Leica Cat#AR9640), Retrieval Solution Low pH (Agilent Cat#GV80511-2) ...
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bioRxiv - Cell Biology 2019Quote: ... Animals for protein loss assay were intracardially perfused with heparinized 0.01 M PBS (10 U ml-1 of heparin, Ratiopharm; ~110 mmHg pressure using a Leica Perfusion One system) for 5–10 min at room temperature until the blood was washed out ...
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bioRxiv - Bioengineering 2021Quote: ... they were intracardially perfused with cold heparinized 0.1 M PBS (10 U/mL of Heparin, Ratiopharm; 100-125 mmHg pressure using a Leica Perfusion One system) for 5-10 minutes at room temperature until the blood was washed out ...
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bioRxiv - Neuroscience 2020Quote: ... frozen with liquid nitrogen and cryo-sectioned (25 μm coronal section thickness for brains and 10 μm axial section thickness for spinal cords) using a cryostat (CM1850, Leica, Wetzlar, Germany) with a microtome blade (A35 ...
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bioRxiv - Neuroscience 2020Quote: ... Coronal 20μm-think brain sections that contained the barrel cortex were cut and collected on-slide (wS1) using a cryostat (Leica, CM3050 S). The slides were then stored at −80°C until further processing ...
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bioRxiv - Neuroscience 2020Quote: ... The brain was thoroughly washed in 0.1 M PBS and thereafter sectioned (40 µm thickness) using a vibratome (Leica VT1000, Leica Microsystems). The sections were stored in 1x PBS at 4°C.
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bioRxiv - Cancer Biology 2019Quote: ... before intracardial perfusion with heparinized 0.01 M PBS (10 U/ml of Heparin, Ratiopharm; 100-125 mmHg pressure using a Leica Perfusion One system) for 5-10 minutes at room temperature until the blood was washed out ...
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bioRxiv - Bioengineering 2022Quote: ... The scaffolds were then stored in 0.15 M NaCl until imaging using a fluorescence macroscope (Macrofluo Z16 Apo, Leica Microsystems, Wetzlar, Germany) with a 0.8X objective.
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bioRxiv - Cell Biology 2023Quote: z-stacks (0.2 to 0.4μm distance between planes) of immunolabelled cells and groups of cells were acquired using an inverted scanning confocal microscope (Leica SP5-SMD), 63x oil objective (1.4NA) ...
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bioRxiv - Neuroscience 2023Quote: ... Tissues were cut into 50 μm slices, images captured using Carl Zeiss Axio Scan microscope (Promenade 10, Jena, Germany) or confocal scanning microscope (DMi8, Leica, Wetzlar, Germany).
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bioRxiv - Microbiology 2019Quote: ... and cut to 5-μm sections (Leica CM3050 S). A double-labeled Arch915 probe in Cy3 (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... and examined under a confocal microscope (Stellaris 5, Leica) at 63x magnification.
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bioRxiv - Neuroscience 2024Quote: ... gel blocks were cut into cuboid shapes (2×2×1.3 mm) with a vibratome (Leica VT1000S, Leica Biosystems GmbH). Scaffolds were then precisely inspected under a light microscope (Olympus BX53 ...
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bioRxiv - Neuroscience 2024Quote: ... gel blocks were cut into cuboid shapes (2×2×1.3 mm) with a vibratome (Leica VT1000S, Leica Biosystems GmbH). Scaffolds were then precisely inspected under a light microscope (Olympus BX53 ...
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bioRxiv - Neuroscience 2021Quote: ... Regularly spaced brain series (5 × 1-in-5) of 30 μm-thick frozen sections were cut in freezing microtome (SM2000R, Leica, Wetzlar, HE, Germany) in the coronal plane ...
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bioRxiv - Neuroscience 2021Quote: ... 40 μm brain slices from animals perfused with 4% paraformaldehyde (in PBS, pH-7.4) were cut with a cryostat (Leica CM 1800; Leica Canada) and used for immunohistochemistry (IHC) ...
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bioRxiv - Cell Biology 2019Quote: ... EM grids impregnated with cells were sandwiched between a 20 μm–thick copper foil and a flat aluminum disc (Engineering Office M. Wohlwend, Sennwald, Switzerland) and frozen with an HPM 010 high-pressure freezer (Leica Microsystems, Wetzlar, Germany) as described (Tsuji et al. ...
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bioRxiv - Pathology 2021Quote: ... was performed on 5µm-thick sections of formalin-fixed lung mounted on charged glass slides using the Leica Bond RX automated system (Leica Biosystems, Richmond, IL). Heat-induced epitope retrieval was conducted by heating slides to 95°C for 15 minutes in EDTA-based ER2 buffer (Leica Biosystems ...
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bioRxiv - Neuroscience 2020Quote: ... 1.0 mm deep with a controlled velocity of 5.0+0.2 m/s and a dwell time of 100 ms using a stereotaxic electromagnetic impactor (Impact one, Leica; Buffalo Grove, IL, USA) equipped with a 5.0 mm flat steel tip ...
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bioRxiv - Genetics 2020Quote: ... Serial cryosections (10 μm for embryos and 14 μm for larvae and adult zebrafish) were obtained in a Leica CM3050 S cryostat (Leica Ltd., Wetzlar, Germany) and stored at −20 °C for further use.
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 400 μm thick horizontal slices containing the cortex and hippocampus were cut with a vibratome (OTS-4500, Electron Microscopy Sciences /Leica VT1000s, Leica Biosystem) under ice-cold ACSF ...
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bioRxiv - Neuroscience 2022Quote: Temporal lobe tissue fixed with 2.5% glutaraldehyde-2% PFA in 0.1 M phosphate buffer (PB) was transversely sectioned at 200 µm using a Leica VT1200S vibratome (Leica Microsystems GmbH, Heidelberg, Germany). Slices were further post-fixed in 2% osmium tetroxide in 0.1 M PB for 1.5 hours at room temperature ...
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bioRxiv - Immunology 2022Quote: ... Approximately 2 μl of each sample was processed by HPF using a Leica EM PACT 2 (Leica, Milton Keynes, UK). After HPF ...
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bioRxiv - Biochemistry 2023Quote: ... Grids were glow discharged in air for 2 min and sample (2 μL) applied in an EM GP2 plunge freezer (Leica) at 4°C and 90% humidity ...
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bioRxiv - Cancer Biology 2023Quote: ... Sections were then subjected to heat induced antigen retrieval and stained for anti-phospho-p44/42 MAPK (ERK1/2) (Thr202/Tyr204) and anti-KI-67 (CST #9027, 1/400 in PBS/2% BSA) on bond RX (LEICA). Appropriate negative and positive controls and isotype controls were included ...
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bioRxiv - Microbiology 2021Quote: ... SARS-CoV-2 Nucleoprotein: 1:2000 (Leica, AR9961) 30minutes ...
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bioRxiv - Cancer Biology 2020Quote: ... Bcl-2 (clone 3.1 mouse monoclonal antibody, Leica) was used at a 1:100 dilution with epitope retrieval solution 2 pre-treatment for 20 min.
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bioRxiv - Cancer Biology 2023Quote: ... using BOND Epitope Retrieval Solution 2 (Leica Biosystems). Detection was achieved using a Bond Polymer Refine Detection® DAB chromogen kit and counterstained with hematoxylin ...
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bioRxiv - Immunology 2023Quote: ... and BOND Epitope Retrieval Solution 2 (Leica AR9640) for MPO antibody ...
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bioRxiv - Pathology 2023Quote: ... speed 2 (Oxford) or 0.5 mm/s (Leica), and blade angle slightly below 0° (Oxford ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were then mounted on aluminum stubs with carbon adhesive tabs and coated with 5 nm of carbon and 5 nm of iridium (Leica ACE 600, Vienna, Austria). SEM images were acquired on a FIB-SEM platform (Helios 5 UX DualBeam Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were mounted on aluminum stubs with carbon adhesive tabs and coated with 5 nm of carbon and 5 nm of iridium (Leica ACE 600, Vienna, Austria). SEM images were acquired on a FIB-SEM platform (Helios 5 UX DualBeam Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... sputter coated with 5 nm of platinum (Leica EM ACE600), and imaged on Hitachi S-4700 scanning electron microscope ...
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bioRxiv - Bioengineering 2021Quote: ... Micrographs were obtained using an SP-5 confocal microscope (LEICA). Primary (I ...
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bioRxiv - Microbiology 2021Quote: ... SP8 or Stellaris 5 microscope (Leica Microsystems, Milton Keynes, UK). Super-resolution (stimulated emission depletion (STED) ...
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bioRxiv - Cell Biology 2021Quote: ... and 5 μm serial sections were prepared (RM2125, Leica, Germany). Sections then underwent either H&E staining (3801698 ...
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bioRxiv - Developmental Biology 2022Quote: ... Samples were sliced into 5 µm by microtome (Leica, RM2135). The sections were dried at 60 °C and then stored at room temperature ...
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bioRxiv - Cancer Biology 2022Quote: ... 5-µm paraffin sections on slides (HistoCore, Leica Biosystems, Germany) were immersed in xylene for 10 minutes ...
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bioRxiv - Microbiology 2022Quote: ... was cut in 5 μm sections using a cryotome (Leica) and mounted onto Superfrost Plus glass slides (Hartenstein) ...
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bioRxiv - Physiology 2023Quote: ... cut into 5-μm sections using a microtome (Leica Biosystems) and stained with hematoxylin and eosin (H&E ...