Labshake search
Citations for Leica :
101 - 150 of 1801 citations for 1 5 Bis 2 2 methyl 1 oxoallyl oxy ethyl dihydrogen benzene 1 2 4 5 tetracarboxylate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2024Quote: ... or EDTA (Bond Epitope Retrieval Solution 2, Leica; CD34, LANA) for 20 min prior to immunostaining ...
-
bioRxiv - Plant Biology 2022Quote: ... high-light illumination of the cells was achieved by exposing the cuvette to white light set to an intensity of 620 μmol photons m-2 s-1 (Leica KL1500 LCD, peak 648 nm, FWHM 220 nm), chosen as the minimal light level necessary to saturate photosynthetic reactions and induce qE activity at the onset of illumination ...
-
bioRxiv - Neuroscience 2020Quote: ... Brains were isolated and immersed in 20% sucrose for 1 day and 30% sucrose for 2 days at 4°C before being sectioned at 40 μm thicknesses on a cryostat microtome (Leica, CM3050s). Brain slices were collected and blocked with blocking solution containing 2% Normal goat serum (v/v) ...
-
bioRxiv - Neuroscience 2021Quote: ... Brains were harvested and stored in the same solution at 4 °C for 2 h before being sliced into 150 μm-thick slices on a vibratome (Leica VT1000S). The slices were stored in 0.1 M glycine and 0.01% sodium azide in 1× PBS at 4 °C until use.
-
bioRxiv - Microbiology 2020Quote: Total cell counts were determined by staining with 4’,6-diamidino-2-phenylindole (DAPI) and visualizing on a Leica DMRX epifluorescence microscope (Leica, Germany). The number of FLA-YM stained cells were determined by enumerating cells which had a positive DAPI and FLA-YM signal (excitation at 405 nm and 488 nm wavelengths ...
-
bioRxiv - Neuroscience 2022Quote: ... post-fixed in the 4% PFA overnight, then embedded in 2% low-melting agarose (Lanza, #50115) and vibratome (VT1200S, Leica, Germany) sectioned at 100μm ...
-
bioRxiv - Developmental Biology 2024Quote: ... the brain was incubated in 4% PFA at 37°C for 2 weeks and divided into 100-μm thick sections by a vibratome (Leica VT200S). Serial brain slices were immediately collected ...
-
bioRxiv - Cancer Biology 2024Quote: ... immunostaining of formalin-fixed paraffin-embedded sections (2-4 μm) was performed with the Bond RX automated stainer (Leica Biosystems, Germany). Antigen retrieval was 20 mins at 100°C using the Bond Epitope Retrieval solution 2 (EDTA based buffer ...
-
bioRxiv - Cell Biology 2024Quote: ... ZO-1 and Claudin-5 were acquired using a Leica SP-8 HCX PL APO CS at 63× magnification (Leica) or a DMI-8 TIRF microscope at 20× magnification (Leica Microsystems) ...
-
bioRxiv - Cell Biology 2022Quote: ... Dried samples were then rotary shadowed with 2 nm of platinum and 4-6 nm of carbon using the ACE600 (Leica Microsystems, Germany). Areas of interest from the resulting platinum replicas were separated from glass with 5% hydrofluoric acid and deposited on carbon-coated EM grids (200 mesh formvar/carbon ...
-
bioRxiv - Cell Biology 2022Quote: ... Dried samples were then rotary shadowed with 2 nm of platinum (sputtering) and 4-6 nm of carbon (carbon thread evaporation) using an ACE600 metal coater (Leica Microsystems, Germany). The resultant platinum replica was floated off the glass with hydrofluoric acid (5%) ...
-
bioRxiv - Microbiology 2024Quote: ... containing 4‘,6-diamidino-2-phenylindole (DAPI) and imaged as midsections by confocal laser scanning microscopy (Leica SP8: Leica Microsystems, Wetzlar, Germany). To prevent cross talk between the channels for the different fluorochromes ...
-
bioRxiv - Cell Biology 2023Quote: ... Dried samples were then rotary shadowed with 2 nm of platinum (sputtering) and 4-6 nm of carbon (carbon thread evaporation) using an ACE600 metal coater (Leica Microsystems, Germany). The resultant platinum replica was floated off the glass with hydrofluoric acid (5%) ...
-
bioRxiv - Neuroscience 2021Quote: ... Slices were visualized on a resonant scanning 2-photon system (Leica) and Z-stack images were taken ...
-
bioRxiv - Cancer Biology 2020Quote: ... followed by pretreatment with Epitope Retrieval Solution 2 (AR9649, Leica Biosystems) for 20 minutes and then blocking buffer for 10-20 minutes ...
-
bioRxiv - Immunology 2023Quote: ... Fura-2-AM was excited using a monochromatic light source (Leica) coupled into an inverted Leica DMI4000B microscope that was equipped with a 20x objective (HCX PL Fluotar 20x ...
-
bioRxiv - Cancer Biology 2023Quote: ... anti-BCL2 (Clone: BCL-2/100/D5; RTU; Leica Biosystems; #PA0117), anti-BCL6 (Clone:LN22 ...
-
bioRxiv - Molecular Biology 2023Quote: ... sputter-coated with 2 nm carbon using ACE600 sputter coater (Leica).
-
bioRxiv - Neuroscience 2024Quote: ... and 2 CaCl2) using a vibratome (VT 1200, LEICA, Wetzlar, Germany) and afterwards incubated in ACSF at 36 °C for 45 min ...
-
bioRxiv - Cancer Biology 2022Quote: ... was incubated at 100 degrees Celsius for 20 minutes (for anti-Cytokeratin 5, Bond Epitope Retrieval 1, pH6.0 (Leica Biosystems, AR9961) was used instead) ...
-
bioRxiv - Neuroscience 2024Quote: ... Brains were then frozen and coronally sectioned at a thickness of 25 um (1:5 series) using a LeicaCM1900 cryostat (Leica Microsystems). Sections were mounted on gelatin-coated slides and coverslipped with VECTASHIELD containing DAPI (catalog no ...
-
bioRxiv - Neuroscience 2024Quote: ... Brains were then frozen and coronally sectioned at a thickness of 25 mm (1:5 series) using a LeicaCM1900 cryostat (Leica Microsystems). Following immunohistochemistry for cFos ...
-
bioRxiv - Neuroscience 2023Quote: ... Stellaris 5 (Leica), or LSM 900 (Zeiss ...
-
Apical expansion of calvarial osteoblasts and suture patency is dependent on graded fibronectin cuesbioRxiv - Developmental Biology 2023Quote: ... the nuclei were stained with DAPI (1:2000) and the images were captured with 63X oil immersion objective by the confocal microscope (Leica STELLARIS 5). The measurements related to cell shape analysis of the nuclei in apical ...
-
bioRxiv - Zoology 2020Quote: ... We obtained 2 μm sections in rotating microtome (RM 2265, Leica, Switzerland), which were stained with hematoxylin and eosin.
-
bioRxiv - Cell Biology 2021Quote: ... Samples were stained in 2% aqueous uranyl acetate and lead citrate (Leica) and imaged on a Hitachi HT7800 transmission electron microscope ...
-
bioRxiv - Pathology 2020Quote: ... Epitope retrieval was performed using Epitope Retrieval solution 2 (Leica Biosystems, Germany) for 20 min ...
-
bioRxiv - Immunology 2021Quote: ... Tubes were loaded into an AFS-2 freeze-substitution machine (Leica Microsystems) and processed at −90°C for 72 h ...
-
bioRxiv - Pathology 2021Quote: ... and ddH2O (2 washes) with a Leica ST4020 Linear Stainer (Leica Biosystems). Tissues next underwent antigen retrieval by submerging slides in 3-in-1 Target Retrieval Solution (pH 9 ...
-
bioRxiv - Plant Biology 2024Quote: ... Sections (2-µm thick) were cut using a UC7&2265 microtome (Leica). To visualize starch granules and protein bodies ...
-
bioRxiv - Plant Biology 2023Quote: ... Sections (2-µm thick) were cut using an UC7&2265 microtome (Leica). To visualize starch granules and protein bodies ...
-
bioRxiv - Neuroscience 2024Quote: ... All procedures were carried out using to Angle 2 software (Leica Biosystems) to set and locate coordinates ...
-
bioRxiv - Microbiology 2023Quote: ... and 2% lead-citrate solution (Leica Ultrostain II, Leica Microsystems, Wetzlar, Germany) for 10 min each ...
-
bioRxiv - Developmental Biology 2023Quote: Worms (young adults) were fixed by HPF with EMPACT-2 (Leica Microsystems) and then freeze substituted in anhydrous acetone containing 1% OsO4 ...
-
bioRxiv - Cell Biology 2023Quote: ... Tubes were loaded into an AFS-2 freeze-substitution machine (Leica Microsystems) and processed at -90°C for 72 h ...
-
bioRxiv - Pathology 2024Quote: ... Epitope retrieval was performed using BOND Epitope Retrieval Solution 2 (Leica Biosystems) for 20 min at 100 °C ...
-
bioRxiv - Microbiology 2024Quote: ... Tubes were loaded into an AFS-2 freeze-substitution machine (Leica Microsystems) and processed at −90°C for 72 h ...
-
bioRxiv - Cell Biology 2024Quote: ... 2-3μl of cells were applied to glass micro slides (Leica 3800220) imaged under cover glass (Leica 3800101 ...
-
bioRxiv - Developmental Biology 2021Quote: ... mouse anti-NK2 homeobox 1 (NKX2-1, 1:500, Leica Biosystems, TTF-1-L-CE), goat anti-interleukin 33 (IL33 ...
-
bioRxiv - Developmental Biology 2024Quote: ... mouse anti-NK2 homeobox 1 (NKX2-1, 1:500, TTF-1-L-CE, Leica Biosystems); Sambucus Nigra Lectin (SNA ...
-
bioRxiv - Cell Biology 2021Quote: ... stained with DAPI (4’,6-diamidino-2-phenylindole) for visualisation of the nuclei and analysed by confocal microscopy using an inverted microscope (Leica DMI6000, objective lens 100×).
-
bioRxiv - Cancer Biology 2024Quote: Cells were seeded on glass bottom dishes then image acquisitions were performed every 2 min for 4 using LiveSR spinning-disk microscope (Leica, Leica Microsystems GmbH, Wetzlar, Germany) with environmental control at 37°C and CO2 provider ...
-
bioRxiv - Neuroscience 2024Quote: ... Sagittal 300-μm-thick slices were cut from the dorsal level of the hippocampus48 at 2-4 mm from midline using a vibratome (Leica VT-1200, Nussloch, Germany). Slices were stored in a reserve chamber containing slicing solution at 34°C for at least 30 min and subsequently at room temperature (20 – 25°C).
-
bioRxiv - Cell Biology 2024Quote: ... 4 to 5-week-old colonies were imaged using the stereo microscope (M205 FA, Leica), and the area was determined by applying thresholding to convert reflected light 8-bit images to 1-bit images and analyzed using Fiji software (ImageJ 1.54f ...
-
bioRxiv - Neuroscience 2021Quote: ... isogenic samples reared in the same environment were highpressure frozen (Leica HPM100 for datasets 1-5 and Leica ICE for datasets 6-8) at different stages of post-embryonic development ...
-
bioRxiv - Neuroscience 2022Quote: ... Brains were then frozen and coronally sectioned at a thickness of 30 μm (1:5 series) using a Leica CM1900 cryostat (Leica Microsystems, Wetzlar, Germany). Fos2A-iCreER+/-::Ai14+/- sections were mounted on gelatin-coated slides and coverslipped with Vectashield containing DAPI (cat ...
-
bioRxiv - Bioengineering 2022Quote: ... the laser scanning setup was chosen (Leica Sp8 SMD, see suppl. video 2): a small square ROI of ca ...
-
bioRxiv - Plant Biology 2021Quote: ... Fluorescent images were observed under confocal laser scanning microscopy (TCS SP5 2; Leica).
-
bioRxiv - Cell Biology 2021Quote: ... for cryosection preparations or in 2% agarose for sectioning with a vibratome (Leica). Sections were subsequently blocked using 1%BSA in TBS containing 0.05% Tween 20 (Sigma ...
-
bioRxiv - Cell Biology 2022Quote: ... glo-2 mutants were phenotyped on a DM5500 wide-field fluorescence microscope (Leica) for the absence of auto-fluorescent and birefringent gut granules ...