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Citations for Leica :
1401 - 1450 of 1455 citations for 2 4 6 Trichlorophenol 13C6 99% 100 Ug Ml In Isooctane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... fluorescence in nIII/nIV at 2 dpf, visualized using a SugarCube LED Illuminator (Ushio America, Cypress CA) on a stereomicroscope (Leica Microsystems, Wetzlar, Germany) and absence of a swim bladder at 5 dpf ...
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bioRxiv - Systems Biology 2024Quote: ... Dried smears were incubated in 70% ethanol for 2 hours and hematoxylin/eosin (H&E) stained using an automatic Leica Multistainer and Coverslipper (Leica ST5020+CV5030, Leica Microsystems, Wetzlar, Germany). Using a scoring system bleeding intensity was evaluated in vaginal smears by microscopy ...
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bioRxiv - Bioengineering 2022Quote: ... The homogeneity of BMP-2 loading inside the film-coated 3D mini-scaffolds was assessed using a BMP-2 loading solution containing 5% of BMP-2Rhod and a fluorescence macroscope (Macrofluo Z16 Apo, Leica Microsystems, Wetzlar, Germany) with a 0.8X objective ...
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bioRxiv - Cancer Biology 2023Quote: ... slides were baked for 2 hours at 65°C for paraffin removal and subsequently loaded onto a Leica BOND RX (Leica Biosystems, Wetzlar, Germany) for tissue rehydration in EtOH and ddH2O ...
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bioRxiv - Cell Biology 2023Quote: ... The polymerized cells were sectioned to a 2 μm depth from the block face using a Leica EM ARTOS 3D ultramicrotome (Leica Microsystems, Wetzlar, Germany) and a diamond knife (DiATOME ...
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bioRxiv - Biophysics 2021Quote: ... and coronal slices of the brain were processed through graded alcohols (70, 90%, 100%) and xylene followed by paraffin wax (LEICA ASP 300; Leica Milton Keynes, UK), and embedded in wax blocks ...
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bioRxiv - Plant Biology 2021Quote: ... 10 µg ml−1 WGA-AF488) and observed with a TCS-SP8 confocal laser-scanning microscope (Leica Microsystems) under the following conditions ...
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bioRxiv - Neuroscience 2019Quote: ... The brain was snap frozen using 2-methylbutane and sliced into 40-50um coronal sections using a cryostat (Leica CM3050S, Leica Biosystems, Buffalo Grove, IL). Slices were mounted on gelatin covered microscope slides and allowed to dry ...
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bioRxiv - Molecular Biology 2020Quote: ... 4 μl of this mixture was back side blotted for 6 s at relative humidity 98% and temperature 19°C on a glow-discharged holey carbon grid (CF-2/1-3C, Protochips) before plunge-freezing in liquid ethane (Leica EM GP2 automatic plunger). Dose-symmetrical tilt series acquisition 51 was performed on an FEI Titan Krios electron microscope operated at 300 kV using a Gatan Quantum energy filter with a slit width of 20 eV and a K2 or K3 direct detector operated in counting mode ...
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bioRxiv - Cancer Biology 2021Quote: ... Agilent M087601-2, used at a dilution of 1/50 for 15mins at room temperature. HIER was performed on-board using Leica ER1 solution (low pH) for 20 minutes ...
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bioRxiv - Immunology 2021Quote: ... After fixation organs were stored in PBS till paraffin embedment and cut of serial sections on a microtome (2-3 µm; Leica RM 2035; Leica Instruments GmbH, Nuβloch, Germany). Sections were stained by hematoxylin and eosin (HE ...
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bioRxiv - Neuroscience 2020Quote: ... and resilient (n=12) mice from experiment 2 were sliced into 300 μm coronal slices on a Leica CM3050 S Research Cryostat (Leica Biosystems, Amsterdam, the Netherlands), with a chamber temperature of −12 °C and an object temperature of −10 °C ...
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bioRxiv - Plant Biology 2022Quote: ... the semi-thin sections (thickness 2 μm) were cut by an automatic semi-thin rotary cutting machine (LEICA RM2265, Leica Microsystems Co., Ltd., Germany) and triangular glass knife ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The growth culture medium was changed every 2-3 days and cell morphology was regularly checked by visual inspection with phase-contrast microscopy (Leica DMIC, Leica Microsystem GMbH, Wetzlar, Germany). When the degree of confluence reaches approximately 80 % (every 4-6 days) ...
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bioRxiv - Neuroscience 2023Quote: ... medial-lateral (ML) coordinate 1.5 mm) using an electromagnetic Leica Impact One Stereotaxic impactor (Leica Biosystems, Richmond, IL, USA) with following parameters ...
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bioRxiv - Molecular Biology 2022Quote: ... All images were taken no longer than 2 hrs after gavage using the wide-field feature of a LEICA DM6 confocal microscope (Leica Microsystems Inc., Buffalo Grove, IL).
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bioRxiv - Molecular Biology 2020Quote: ... the individual primordial oocyte was collected from 2-3 adjacent tissue sections to ensure that one full cell was included (Leica Microsystems, Buffalo Grove, IL, USA).
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bioRxiv - Neuroscience 2020Quote: ... The slices of cochlear nucleus were cut at 220-300 μm in ice-cold sucrose solution on a vibratome (Leica VT1200S or Campden 7000smz-2). Slices were transferred into standard artificial cerebrospinal fluid (ACSF ...
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bioRxiv - Biochemistry 2022Quote: ... and 380 nm (F380) and λem = 510 nm in a Leica DMi-8 microscope equipped with a Fura-2 filter (Leica Microsystems, Buffalo Grove, IL, USA). Cells were followed through additions of CGP (10 μM) ...
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bioRxiv - Neuroscience 2024Quote: High-pressure freezing and freeze-substitution (HPF-FS) were performed at Harvard Medical School using the Leica EM ICE and EM AFS-2 (Leica Microsystems Inc, Buffalo Grove, IL). Whole brains were immediately frozen upon dissection ...
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bioRxiv - Plant Biology 2023Quote: ... mature rice pollen grains were stained with 2 % I2-KI (w/v) solution and observed under a bright field microscope (Leica DM IL LED S-80) according to Chhun et al. ...
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bioRxiv - Biochemistry 2024Quote: ... and finally incubated with 3 μM MitoSOX Red for 10 min and fixed with paraformaldehyde (2%) and analyzed by fluorescence microscopy (Leica TCS SP5, Buffalo Grove, IL).
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bioRxiv - Immunology 2021Quote: Kidneys were isolated from resting mice after PBS perfusion and fixed O/N in 20 mL of 10% Neutral Buffered Formalin (Leica). Fixed kidneys were paraffin-embedded using a Shandon Citadel 1000 tissue processor (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... A 1.5 × 1.5 mm area above A1 centered at −2.5 mm AP and 4.0 mm ML from bregma [16] was opened and the dura removed under a microscope (S6, Leica Mycrosystems). The tetrode device containing 8 NiCr electroplated tetrodes was attached to a micrometric stereotaxic arm (1760 ...
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bioRxiv - Cell Biology 2021Quote: ... INS-1E cells were incubated with HO-PI for 15 min (5 mg/ml) and observed using a confocal laser-scanning microscope (Leica SP5 ...
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bioRxiv - Bioengineering 2019Quote: Images of cell clusters stained with 2.5 μg/mL DTZ (MilliporeSigma; 194832) were taken with an inverted light microscope (Leica DMi1).
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bioRxiv - Cell Biology 2020Quote: ... A 250-µl dispase solution (2.4 U/ml) was then gently pipetted into the opening and imaging at 20× was started in an inverted microscope (DMi8, Leica) acquiring 1 image per minute for 45 min to 1 hr.
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bioRxiv - Plant Biology 2022Quote: ... roots were dipped in propidium iodide (PI) solution (10 µg/ml) for 30 secs to stain the root cell wall (Leica SP8 confocal microscope ...
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bioRxiv - Plant Biology 2023Quote: ... cell walls were labelled with propidium iodide (5 µg/mL) and imaged using a 20x PLAN APO multi-immersion objective (Leica), with λexc = 488 nm and λem = 551-651 nm ...
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bioRxiv - Cell Biology 2024Quote: ... 13.8nL of cell suspension at 100.106 cells per ml were injected in the duct of Cuvier of the embryos under the M205 FA stereomicroscope (Leica). Polyacrylamide microgel beads at a respective stock concentration of 1.6kPa-Alexa Fluor 633 at 83 x 106 beads/ml) ...
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bioRxiv - Cell Biology 2023Quote: ... 13.8nL of cell suspension from confluent T25 flasks (50×106 cells per ml approx.) were injected in the duct of Cuvier under a M205 FA Fluorescence stereomicroscope (Leica), as previously described (Stoletov et al. ...
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bioRxiv - Biochemistry 2023Quote: ... In the first channel, a 1-ml syringe tip (Braun, 9166017V) was used to connect the perfusion needle (Leica, 39471024) and the reference tube (Ismatec Reglo ...
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bioRxiv - Microbiology 2023Quote: ... or FM464 (5 µg/mL) dyes for 30 min and imaged by phase-contrast and fluorescence using an inverted DeltaVision Elite Microscope (Leica) equipped with an UPLFLN100XO2/PH3/1.30 objective (Olympus) ...
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bioRxiv - Cell Biology 2023Quote: ... and 18.4 nL of a cell suspension at 108 cells/mL were injected in the duct of Cuvier of the embryos under a M205 FA stereomicroscope (Leica). Injected embryos were maintained at 28°C in between injection and imaging ...
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bioRxiv - Developmental Biology 2024Quote: ... or 1 mg/mL SCRI Renaissance 2200 dye (SR2200) (Renaissance Chemicals) and imaged in a Leica SP8 laser-scanning confocal microscope (Leica) using a hybrid detector counting mode and the following settings ...
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bioRxiv - Cell Biology 2020Quote: ... Sections were then washed 2 times for 8 minutes in DPBS containing 0.3% Triton X100 and mounted on slides (Leica Surgipath® X-tra™ Adhesive, 3800202) using Vectashield Antifade Mounting Medium (Vector Laboratories ...
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bioRxiv - Plant Biology 2021Quote: ... tumefaciens clones carrying the appropriate BiFC plasmids using a 1 mL needleless syringe and imaged two days post-infiltration with a Leica TCS SMD confocal microscope (Leica Microsystems) using the preset settings for YFP (Ex ...
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bioRxiv - Cell Biology 2021Quote: ... and xz images were acquired in the presence of the membrane dye CellMask (5 mg/ml, Life Technology) using Leica TCS SP5X confocal microscope (Leica Microsystems). The MFI of Fluo-4 in the cytoplasmic region in individual cells was measured using the NIH ImageJ software ...
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bioRxiv - Immunology 2022Quote: ... Larvae were then immediately mounted in 1% low-melting agarose containing 40 μg/ml tricaine and live imaged overnight using confocal microscopes (Zeiss 780 NLO or Leica SP8). The fluorescence intensity over time was calculated using SUM intensity projections ...
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bioRxiv - Cancer Biology 2019Quote: ... the tip of a syringe (cut from a 1 ml syringe-Braun, 9166017V) was used to connect the perfusion needle (Leica, 39471024) to the tubing ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were then incubated in 1 mL experimental buffer and placed inside the chamber of a Leica DMi‐8 microscope equipped with a Fura2 filter (Leica Microsystems) and a 40× objective ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were mounted in mowiol/DABCO (25mg/mL) and imaged on a macroscope (MVX10 olympus) or an epifluorescence microscope (LEICA DM6000) using X 40 and X63 immersion objectives ...
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bioRxiv - Bioengineering 2023Quote: ... one ending of the tube was connected to the tip of a syringe (cut from a 1 ml syringe; Braun, 9166017V) which held the perfusion needle (Leica, 39471024), and the other ending was immersed in the solution chamber where the animal was placed ...
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bioRxiv - Cancer Biology 2023Quote: ... at a final concentration of 5 µg/mL for 20 minutes at 37 °C prior to live cell imaging by epifluorescence (Leica DMI6000B). Cells were counted as DAPI-positive if the nucleus showed bright staining and as apoptotic if there was characteristic nuclear fragmentation ...
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bioRxiv - Developmental Biology 2019Quote: ... and reporter activities from 56 hpf to 144 hpf was performed using a Leica DMi8 combined with a CSU-X1 (Yokogawa) spinning at 10 000 rpm, 2 simultaneous cameras (TuCam Flash4.0, Hamamatsu) and a water immersion objective (Leica 20X, N.A. 0.75 or Leica 40X, N.A. 1.1). 1ms exposure was used for red blood cells imaging and 20ms exposure for valve structure and reporter activity experiments ...
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bioRxiv - Plant Biology 2022Quote: ... high-light illumination of the cells was achieved by exposing the cuvette to white light set to an intensity of 620 μmol photons m-2 s-1 (Leica KL1500 LCD, peak 648 nm, FWHM 220 nm), chosen as the minimal light level necessary to saturate photosynthetic reactions and induce qE activity at the onset of illumination ...
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bioRxiv - Cell Biology 2019Quote: ... Animals for protein loss assay were intracardially perfused with heparinized 0.01 M PBS (10 U ml-1 of heparin, Ratiopharm; ~110 mmHg pressure using a Leica Perfusion One system) for 5–10 min at room temperature until the blood was washed out ...
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bioRxiv - Cancer Biology 2024Quote: ... Permeabilized cells were probed for SMAD2/3 (#AF3797, R&D Biosystems, 10μg/mL) and imaged on an SP8 white laser confocal microscope (Leica Biosystems, Wetzlar, Germany). The following antibodies were used for additional immunofluorescence assays ...
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bioRxiv - Physiology 2023Quote: ... Particles were manually offered to the coral fragments in individual feeding chambers (200 mL) under a stereomicroscope (Leica Microsystems GmbH, Leica EZ4, Germany). The microdebris was sampled from the treatment tanks to provide particles covered with a biofilm ...
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bioRxiv - Physiology 2023Quote: ... Particles were manually offered to the coral fragments in individual feeding chambers (200 mL) under a stereomicroscope (Leica Microsystems GmbH, Leica EZ4, Germany). The microdebris was sampled from the treatment tanks to provide particles covered with a biofilm ...