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Citations for Leica :
1351 - 1400 of 1645 citations for 6 Bromo 4 5 dihydro 1H benzo b azepin 2 3H one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... The target probe was validated on reproductive tracts from mice experimentally inoculated with Chlamydia muridarum strain Nigg.5 Slides were stained on an automated stainer (Leica Bond RX, Leica Biosystems) with RNAscope 2.5 LS Assay Reagent Kit-Red (322150 ...
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bioRxiv - Neuroscience 2019Quote: ... Representative images of further experiments investigating neurite outgrowth in differentiated CTX0E16 cells were acquired using a Leica SP-5 confocal microscope with a 100x oil-immersion objective (Leica microsystems). Z-stacks of 5 individual cells per condition across three biological replicates were obtained for statistical data analysis ...
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bioRxiv - Neuroscience 2020Quote: ... and 25 mM dextrose) with bubbling 95% O2 and 5% CO2 using a vibratome (VT1000 S, Leica Microsystems, Buffalo Grove, IL). Acute bilateral fastigial nuclei were quickly excised with a sharp knife from 2 or 3 cerebellar slices and were then enzymatically digested with cysteine (2 mM)-supplemented papain (40 U/ml ...
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bioRxiv - Developmental Biology 2021Quote: ... For immunohistochemistry 5 μm thick serial sections were cut at −23°C using a Leica CM3050 cryostat (Leica Microsystems, Wetzlar, Germany) and collected in Superfrost® Plus slides (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: All experiments were imaged using a Leica SP-5 confocal microscope using a Plan-Apochromatic 63x 1.40 NA oil-immersion objective (Leica microsystems, 506210). Fluorophores were excited using a 100 mW Ar laser (458 ...
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bioRxiv - Plant Biology 2019Quote: ... The root segments were embedded in agarose 5% and cut into 25 μm radial sections with a Leica VT1200S Leica vibratome (Leica Microsystems). Sections were categorized according to their distance (100 ...
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bioRxiv - Physiology 2021Quote: ... The lung tissue sections of 3-5 micron were stained with H&E stain and were microscopically analyzed using microscope (Make: Leica DM1000 LED; Camera: Leica MC170HD). The images were taken with 10X objective lens (Magnification X 100 ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were mounted on an aluminum stub with a silver lacquer and sputtercoated with a 5 nm platinum layer using EM ACE600 (Leica Microsystems). Acquisitions were performed using a GeminiSEM 500 (Zeiss).
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bioRxiv - Cell Biology 2022Quote: ... lensectomy and sclerectomy were performed and retinas embedded in 5% agar and 150 µm sections made using a fully automated VT1200S vibrating blade microtome (Leica Biosystems). Sections were cultured in retinal media (DMEM extra (Sigma ...
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bioRxiv - Microbiology 2022Quote: ... at 5 µm in thickness with a tungsten carbide blade (SL-T30, SECTION LAB Co. Ltd) using a cryostat system (Leica CM1850). The undecalcified sections attached to the adhesive film were immerged in 100% ethanol for 30 sec to remove the compound and air-dried ...
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bioRxiv - Neuroscience 2022Quote: ... Their brains were quickly dissected out and stuck on a 5 mm agar stage before being mounted on a vibratome (Leica VT1200S) equipped with a sapphire blade ...
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bioRxiv - Neuroscience 2022Quote: ... and Homo (N=5 males) Gcg-Cre rats from the Janvier colony were cut into 50 μm coronal slices using a cryostat (Leica CM3050S). The cNTS was then microdissected using biopsy punches (Integra Miltex ...
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bioRxiv - Physiology 2022Quote: ... then incubated with serum-free protein block for 5 minutes (Novocastra Protein Block Protein Block Serum Free, Leica Biosysems, cat# RE7102), followed by incubation with rabbit anti-FABP4 polyclonal antibody (adipose ...
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Apical expansion of calvarial osteoblasts and suture patency is dependent on graded fibronectin cuesbioRxiv - Developmental Biology 2023Quote: ... Confocal images were captured at 63X oil immersion objective on Leica STELLARIS 5 (Leica; HC PL APO 63x/1.40 OIL CS2) using Application suite X software ...
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bioRxiv - Synthetic Biology 2023Quote: ... The frozen samples were transferred to 1% Osmium tetroxide and 0.1% uranylacetate in acetone and freeze substituted using a Leica EM AFS2 freeze substitution processor at – 90°C with a slow warm up of 5°C per hour up to 0°C (Leica Microsystems). As osmium becomes increasingly reactive at higher temperatures we removed the samples from the AFS2 at 0°C to preserve the “crispiness” of the staining ...
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bioRxiv - Cell Biology 2023Quote: ... Slices were imaged with a laser scanning confocal Stellaris 5 or TCS SP8 gSTED equipped with a white light laser and a 405-diode ultraviolet laser (Leica Microscope). Image acquisition was done using the LAS-X software in accordance with the Nyquist sampling sequential mode by exciting the fluorophores and collecting their signal with hybrid detectors (HyDs).
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bioRxiv - Cell Biology 2023Quote: ... All confocal images were acquired using the Leica TCS SP8 or Stellaris 5 confocal microscope with the Leica LAS X software (Leica Microsystems). Following objectives were used with the Leica TCS SP8 ...
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bioRxiv - Cancer Biology 2023Quote: ... at a final concentration of 5 µg/mL for 20 minutes at 37 °C prior to live cell imaging by epifluorescence (Leica DMI6000B). Cells were counted as DAPI-positive if the nucleus showed bright staining and as apoptotic if there was characteristic nuclear fragmentation ...
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bioRxiv - Cancer Biology 2022Quote: ... was incubated at 100 degrees Celsius for 20 minutes (for anti-Cytokeratin 5, Bond Epitope Retrieval 1, pH6.0 (Leica Biosystems, AR9961) was used instead) ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were placed in a 37°C chamber incubator supplemented with 5% CO2 mounted on Leica TCS SP8X microscope system (Leica Microsystems).
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bioRxiv - Neuroscience 2023Quote: ... The STG was then illuminated for 5-7 mins using a Texas red filter set (560 ± 40 nm wavelength; Leica Microsystems). Complete photoinactivation was confirmed when the LP membrane potential reached 0 mV.
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bioRxiv - Microbiology 2023Quote: ... as described above, the cells were incubated (37°C, 5% CO2) inside the Mica Microhub system using the internal incubator (Leica microsystems). Videos were analysed using the complementary software (Leica microsystems).
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bioRxiv - Neuroscience 2023Quote: ... The STG was then illuminated for 5-7 mins using a Texas red filter set (560 ± 40 nm wavelength; Leica Microsystems). Complete photoinactivation was confirmed when the LP membrane potential reached 0 mV ...
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bioRxiv - Plant Biology 2023Quote: Lengths of root RAM and EZ regions and root hairs in 5-d-old seedlings grown on vertical MS medium without sucrose were measured under a Leica microscope (Leica DM5000). The data were analyzed using the ImageJ software (http://rsb.info ...
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bioRxiv - Developmental Biology 2023Quote: ... before being embedded in 5% low-melting agarose and sectioned into 100-400 μm-thick slices using a Vibratome (Leica, VT1200). Slices were blocked and permeabilized using PBST supplemented with 5% donkey serum and 0.3% Triton X at room temperature for 30 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... the brain was briefly submerged in ice cold cut solution bubbled with carbogen (95% oxygen, 5% CO2) and then sectioned into 300μm sagittal sections (Leica VT1200S vibratome) in bubbled ice-cold cut solution ...
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bioRxiv - Evolutionary Biology 2023Quote: ... LEGE 07298 and Phormidesmis priestleyi ULC 007 stained with either calcein or 5-CF were acquired with an inverted confocal laser scanning microscope (CLSM; Leica SP8) using a HC PL APO x63/1.40 oil objective ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and 5% H2O) under liquid nitrogen and placed in an automatic freeze substitution system (Leica AFS2, Leica Microsystems, precooled to −90 ℃) for 24 h ...
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bioRxiv - Neuroscience 2024Quote: ... the lumbar spinal cord from L3-5 was mounted on an agarose mold and the dorsal halve was micro dissected on a vibratome (Leica Microsystems). Next ...
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bioRxiv - Cell Biology 2024Quote: ... Paraffin-embedded WAT was sliced into 5-μm sections using a ThermoScientific HM 355S Automatic Microtome and mounted on Surgipath X-tra Microscope Slides (Leica Biosystems).
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bioRxiv - Cell Biology 2024Quote: ... Samples were mounted on an aluminum stub with a silver lacquer and sputtercoated with a 5 nm platinum layer using EM ACE600 (Leica Microsystems). Acquisitions were performed using a GeminiSEM 500 (Zeiss).
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bioRxiv - Neuroscience 2019Quote: ... The brain was snap frozen using 2-methylbutane and sliced into 40-50um coronal sections using a cryostat (Leica CM3050S ...
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bioRxiv - Microbiology 2020Quote: ... After immunolabelling the grids were embedded with a mixture of 50% methylcellulose 2% and 50% sucrose 2.3M and then vitrified by plunge freezing with EMGP plunge freezer (Leica) at 30 °C and 90% humidity.
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bioRxiv - Plant Biology 2021Quote: ... and the following 2 cm of stem were collected and 100 µm sections were generated using a vibratome (Leica VT1000S ...
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bioRxiv - Biochemistry 2022Quote: ... The ibidi dishes were mounted for time-lapse microscopy 2 h after induction on a Delta Vision microscope (Leica). Mitotic progression was monitored by taking images every 5 min for 20 h at different areas of the wells and three z-stacks (5 μm apart) ...
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bioRxiv - Neuroscience 2022Quote: ... At least 3 different fields from 2 slices per experiment were analyzed by using LAS AF Lite software (Leica). The fluorescence signal corresponding to the PLP-DsRed OLs was quantified by ImageJ software and data were expressed as arbitrary units of fluorescence for each experimental situation ...
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bioRxiv - Developmental Biology 2021Quote: ... and photographed with an Aperio AT 2 Leica Scanner and an Aperio Scan Scope Console program (Leica, Wetzlar, Germany) available at the Northern Finland Biobank Borealis ...
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bioRxiv - Molecular Biology 2019Quote: ... after seeding and imaged regularly with a Leica DM IL LED system equipped with a HI PLAN I Phase 2 40x/0.50 (Leica) objective lens.
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bioRxiv - Pathology 2022Quote: ... Super-resolution images were recorded on the confocal laser scanning microscopy LSM 980 equipped with Airyscan 2 (Leica, Germany).
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bioRxiv - Cancer Biology 2022Quote: ... using the following conditions: antigen retrieval at 100ºC for 20 min with Epitope Retrieval Solution 2 (AR9640, Leica Biosystems); primary antibody incubation with anti-P53 ...
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bioRxiv - Developmental Biology 2020Quote: Embryos or neural tube explants are fixed in 3.7% formaldehyde for 2 hours and sliced using a vibratome (Leica). Proteins were detected on 50 μm vibratome sections ...
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bioRxiv - Cell Biology 2020Quote: ... Sections mounted on Pioloform filmed copper grids prior to staining with 2% aqueous uranyl acetate and lead citrate (Leica). Ultrathin sections were examined on a Phillips CM 100 Compustage (FEI ...
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CXCR4-binding PET tracers link monocyte recruitment and endothelial injury in murine atherosclerosisbioRxiv - Pathology 2020Quote: ... Collected aortas were placed between 2 clear sheets and imaged by M205 FA stereoscope system with Cy5 channel (Leica). CXCR4 co-staining was done after fixation using whole mount staining protocol described above.
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bioRxiv - Cell Biology 2019Quote: ... Cells were counterstained with 4,6-diamidino-2-phenylindole (DAPI) and imaged with a Leica TCS SP5 Confocal Laser Scanning Microscope (Leica) using a 40X water immersion objective.
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bioRxiv - Genetics 2021Quote: ... Animals stained were mounting in agarose 2 X pads with a sodium azide drop (0,05 M) to count amyloid deposits by fluorescent microscopy (DM2500, Leica) and quantify stained area of worms by digital dissection microscope (DMD108 ...
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bioRxiv - Cell Biology 2020Quote: ... then transferred to brass specimen carriers and high pressure frozen with an EMPACT 2 (Leica Biosystems, Buffalo Grove, IL).
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bioRxiv - Immunology 2021Quote: ... Semi-thin (2 μm) sections were cut with glass knifes (KnifeMaster II, LKB Bromma 7800) on an ultramicrotome (Leica Ultracut ...
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bioRxiv - Neuroscience 2023Quote: ... tissue was immersed in 30% sucrose for 2 days followed by embedding in Tissue-Tek OCT and sectioning of 50μm slices in a cryostat (Leica). Staining was done as described (Licht et al. ...
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bioRxiv - Plant Biology 2022Quote: ... The measurements were performed 2 to 3 days after infiltration using a SP8 laser scanning microscope (Leica Microsystems GmbH) with LAS AF and SymPhoTime (PicoQuant ...
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bioRxiv - Neuroscience 2023Quote: ... and MgCl2 7) for 2 minutes and subsequently sliced in the same solution using a vibrating microtome (Leica VT1200S). Slices were allowed to recover for 1 hour at 33°C in carbongenated standard aCSF which contained (mM) ...