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Citations for Leica :
1251 - 1300 of 2162 citations for 3 4 Hydroxy 5 isopropyl 6 oxo 1 6 dihydro pyrimidin 2 ylsulfanyl propionic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... Stained slides were placed at 4°C for at least 24 hrs before imaging immunofluorescence using a light microscope (Leica DMi8, Leica Microsystems). To establish relatively quantitative significance ...
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bioRxiv - Neuroscience 2024Quote: ... Mouse brains were post-fixed in 4% PFA overnight and sectioned coronally at 50 µm thickness (approximately 240 sections per brain) on a vibratome (Leica VT1200S). Brain sections containing regions of interest were permeabilized in 1% Triton X-100 in TBS and blocked in 5% normal goat serum ...
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bioRxiv - Neuroscience 2024Quote: ... brains were post-fixed in 4% PFA at 4 °C and then rinsed in PBS before being sectioned at a thickness of 60 µm using a vibratome (Leica Biosystems). These sections were then mounted onto Superfrost Plus slides (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2024Quote: ... Brains were removed and postfixed overnight at 4°C and then cut into 50 µm coronal sections using a vibratome (#TPI1000, Ted Pella, or VT1000P, Leica). Sections were collected into cryoprotectant (25% glycerol ...
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bioRxiv - Developmental Biology 2024Quote: ... the samples were transferred to 30% Sucrose solution overnight at 4°C before OCT (Sakura) inclusion for performing 20 µm coronal sections using Cryostat (Leica Biosystems). Cryostat sections were washed three times with PBST (1x PBS with Tween 20 ...
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bioRxiv - Neuroscience 2021Quote: ... Spheres were allowed to grow for 5 weeks and were photographed using an inverted bright-field microscope (Leica DMIRBE). Viable spheres were recovered by centrifugation at 100g for 5min at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... 5-day old Arabidopsis seedling roots expressing AtC53-mCherry were cut and high-pressure frozen (EM PACT2, Leica, Germany), prior to subsequent freeze substitution in acetone containing 0.4% uranyl acetate at −85 °C in an AFS freeze-substitution unit (Leica ...
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bioRxiv - Developmental Biology 2021Quote: Embryos of targeted injections of DshDEP+ into the neural fold were imaged using the Stellaris 5 confocal system with the HC APO L 10×/0.30 NA water immersion objective (Leica). The sections of these embryos were imaged using the LSM 980 confocal system with the LD LCI Plan-Apochromat 25×/0.80 NA oil immersion objective (Zeiss).
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bioRxiv - Cell Biology 2020Quote: Cells seeded at low density on glass bottom Willco dishes or cell-collagen cultures were monitored by temperature- and CO2-controlled bright-field inverse microscopy (37°C, 5%; air objectives, 10×, NA 0.20, Leica; 20× ...
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bioRxiv - Pathology 2020Quote: Frozen tissue blocks were sectioned at 5 µm thickness in a Leica CM1850 cryostat (Leica Microsystems GmbH, Wetzlar, Germany) at -20C ...
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bioRxiv - Zoology 2021Quote: ... Tissue sections with 5 μm thickness were cut using a microtome (Leica RM2235, Leica Biosystems Inc., Buffalo Grove, USA), fixed on slides ...
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bioRxiv - Cancer Biology 2022Quote: ... Invading cells were imaged with a sCMOS Orca-Flash 4.0LT camera (Hamamatsu) coupled 5 to an inverted DMi8 microscope (Leica), capturing serial z-stacks every 10 µm with a 10× objective (dry ACS APO 10x/NA 0.3) ...
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bioRxiv - Cell Biology 2022Quote: ... The cells were observed in 5% CO2 at 37°C using an HC PL APO 63 ×/1.40 oil objective (Leica). FAOBlue was excited at 405 nm ...
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bioRxiv - Neuroscience 2021Quote: ... bubbled with 95% O2-5% CO2 and sliced using a Vibratome (Leica VT1200, Leica Microsystems Inc, Buffalo Grove IL) except for experiments in which high Ca2+ concentrations were used ...
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bioRxiv - Neuroscience 2021Quote: ... The paraffin-embedded brains were sectioned in the coronal plane at 5 µm using a microtome (Leica RM 2265). Sections were collected at 40 μm intervals ...
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bioRxiv - Cell Biology 2022Quote: ... Fluorescence images of stained myotubes were obtained in 5 different areas using a DMI 3000 B microscope (Leica, Germany) and analyzed using ImageJ 1.52 software (National institutes of Health ...
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bioRxiv - Physiology 2021Quote: ... Undecalcified 5 μm and 10 μm thick longitudinal sections were obtained using a microtome (RM2255, Leica Biosystems., IL, USA). 5 μm sections were stained with Goldner Trichome and at least two nonconsecutive sections per sample were examined for measurement of cellular parameters ...
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bioRxiv - Cell Biology 2020Quote: ... The cells were observed in 5% CO2 at 37°C using an HC PL APO 63x/1.40 oil objective (Leica). Multichannel time-lapse images were acquired in 4 fields each with 4 averages per frame in 1-min or 1.5-min intervals ...
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bioRxiv - Immunology 2021Quote: ... Organs included in paraffin were cut to a thickness of 5 microns (Microtome Leica RM2245 of disposable metal blades) and placed in a flotation solution in a water bath and then fixed on a slide sheet ...
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bioRxiv - Plant Biology 2020Quote: ... The embedded blocks were transversely sectioned at 5 to μm thickness using a Leica RM2125RT rotary microtome (Leica Microsystems) on RNase-free polyethylene naphthalate PEN membrane slides (Leica) ...
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bioRxiv - Neuroscience 2020Quote: ... At least 5-10 flies per genotype were scanned using the Confocal Laser Scanning Platform Leica SP8 (Leica, Germany). Samples were excited with an argon laser at 488nm (20% tube current) ...
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bioRxiv - Biochemistry 2023Quote: ... The paraffin-embedded tissues were sectioned (5 µm thick) with a semi-automated rotary microtome (Leica Microsystems, Wetzlar, Germany) and placed on microscope glass slides coated with poly-L-lysine ...
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bioRxiv - Zoology 2023Quote: ... Paraffin blocks were sectioned (5-7 μm, 20-40 sections per block) using a microtome (Leica RM 2125RT, Germany). Histological sections were stained with hematoxylin and eosin ...
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bioRxiv - Neuroscience 2022Quote: ... mice were anaesthetized using 2.5% isoflurane and 1.5 l/min air for 5 min and impacted directly over their intact head using an electromagnetic impactor (Leica Impact One Stereotaxic impactor ...
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bioRxiv - Physiology 2022Quote: ... The samples were imbedded in paraffin followed by serial sectioning at 5 µM thickness using the Leica microtome (Leica). The sections were deparaffinized ...
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bioRxiv - Biophysics 2023Quote: ... They were imaged with sequential z-stacks using a similar procedure as for the 3D colonies but with an 5× air objective (Nplan5×0.12PHO, Leica). The voxel size in (x,y,z ...
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bioRxiv - Neuroscience 2024Quote: ... PL Apo 40x/0.85 CORR CS and PL APO 63x/1.40 oil CS2 and Leica Stellaris 5 confocal microscopes (Leica Microsystems) equipped with a HCPL FLUOTAR 10x/0.30 ...
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bioRxiv - Neuroscience 2024Quote: ... saturated with 95 % O2 and 5 % CO2 to obtain 250 μm-thick coronal sections with a vibratome (Leica VT1200S). Slices were transferred and incubated in a holding chamber containing (ACSF ...
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bioRxiv - Developmental Biology 2024Quote: Fixed samples were imaged in a Leica Stellaris 5 upright confocal microscope using a HC PL APO 40x/1,1 Water CORR CS2 objective (#506425, Leica). The microscope was operated by the LAS X (v4.5.0.25531 ...
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bioRxiv - Immunology 2024Quote: ... The stage-top chamber was set onto the stage of a Stellaris 5 confocal microscope (Leica Microsystems, Deerfield, IL). Transmitted (brightfield ...
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bioRxiv - Biophysics 2023Quote: ... Stellaris 5 up-right system using either a HC APO L U-V-I 10x/0.30 NA WATER (Leica) or a HC FLUOTAR L VISIR 25X/0.95 NA WATER (Leica ...
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bioRxiv - Bioengineering 2023Quote: ... the grips were attached to a custom mechanical testing rig mounted on an upright confocal microscope (Leica STELLARIS 5) in a bath with 1× PBS (Fig ...
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bioRxiv - Bioengineering 2022Quote: ... and placed on a microscope slide to image with a confocal microscope (Leica Stellaris 5 confocal with DMI 8). The settings for fluorescent imaging were calibrated using a positive control ...
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bioRxiv - Bioengineering 2023Quote: ... Images (n = 5) of S-shape embedded filaments were taken with a confocal microscope (SPX8, Leica Microsystems, The Netherlands) and analyzed with ImageJ ...
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bioRxiv - Molecular Biology 2024Quote: ... the cells were cultured overnight and imaged with a Leica STELLARIS 5 Confocal Microscope (Leica Microsystems Inc., Deerfield, IL).
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bioRxiv - Neuroscience 2024Quote: ... Recording was performed using a CMOS camera (acA2040-90umNIR, Basler) and a ×5 0.15-NA objective (HCX PL FLUOTAR, Leica). Images were acquired with pixel resolution of 3.19 μm/pixel and image size of 1024*1024 at 50 frames per second ...
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bioRxiv - Molecular Biology 2024Quote: ... They were then embedded in paraffin before cutting into 5 µm sections using a microtome (Leica biosystems, Wetzlar, Germany). After deparaffination ...
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bioRxiv - Neuroscience 2024Quote: ... 300-320 mOsmol/kg) saturated with a mix of 95% O2 and 5% CO2 using a VT1000S vibratome (Leica). Slices were transferred to a submerged chamber and continuously perfused with artificial CSF (in mM ...
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bioRxiv - Neuroscience 2024Quote: ... The lumbar spinal cord (L1-5) was sectioned transversely (300µm) in dissection solution using a vibrating microtome (Leica Microsystems). Slices were immediately transferred to recording artificial cerebrospinal fluid (ACSF ...
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bioRxiv - Neuroscience 2024Quote: ... 1.2 NaH2PO4 and 0.5 CaCl2 bubbled with 95% O2/5% CO2 (pH of 7.4) using a vibratome (VT1200S, Leica Biosystems). Coronal slices (280 µm thick ...
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bioRxiv - Neuroscience 2024Quote: ... 20 mM choline bicarbonate) saturated with carbogen (5% CO2 and 95% O2) using a vibratome (Leica VT 1200 S). 250 μm brain slices were incubated in 31°C ACSF solution (119 mM NaCl ...
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bioRxiv - Neuroscience 2024Quote: ... Lumbar spinal cords (L3-5) were harvested and transversely sectioned using a LEICA CM3050 S Cryostat (LEICA, Wetzlar, Germany). For immunostaining ...
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bioRxiv - Neuroscience 2024Quote: ... The bulbs were embedded in 5 % agarose and cut coronally into 50 µm slices using a vibratome (VT1000S Leica).
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bioRxiv - Bioengineering 2024Quote: ... Images (n = 5) of S-shape embedded filaments were taken with a confocal microscope (SPX8, Leica Microsystems, The Netherlands) and analyzed with ImageJ.
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bioRxiv - Cell Biology 2024Quote: ... Image acquisition of fixed embryo and egg chamber samples was performed on an inverted Stellaris 5 confocal microscope (Leica) with a 63x/1.4 NA oil immersion objective ...
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bioRxiv - Cell Biology 2020Quote: Sample blocks were obtained by freeze substitution of the cell pellets in 0.5 % uranyl acetate diluted from a 20 % stock in methanol and 3 % double distilled H2O in acetone in a Leica AFS1 (Leica Microsystems GmbH, Wetzlar, Germany). Temperature was set to −130 °C for the first 2 hours and then raised to −90 °C with a 20 °C/hr gradient ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Samples were imaged in brightfield using a Point Grey Flea®3 color camera (Teledyne FLIR, Wilsonville, OR) mounted on a Leica S8 APO stereo microscope (Leica Camera, Wetzlar, Germany). Representative images are shown.
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bioRxiv - Pathology 2021Quote: Four micron thick sections of left and right ventricular FFPE tissue blocks per decedent were cut and labeled using an automated staining platform (Leica Bond 3, Leica Biosystems, Buffalo Grove, IL) with the following monoclonal antibodies against ...
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bioRxiv - Neuroscience 2021Quote: ... Brains were rinsed 3 times in phosphate buffered saline and 50 μM coronal brain slices were obtained using a vibratome (Leica VT1000, Buffalo Grove, Il), without embedding the tissue ...
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bioRxiv - Bioengineering 2022Quote: ... Samples were washed an additional 3 times in 100% ethanol for 15 minutes each and dried using a Leica EM CPD030 critical point dryer (Leica Microsystems, Buffalo Grove, IL) with CO2 as the transitional medium ...