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Citations for Leica :
1251 - 1300 of 1409 citations for 2 1H Imidazol 1 yl 6 methyl 4 pyrimidinemethanamine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... Sections were stretched with chloroform to eliminate compression and mounted on Pioloform filmed copper grids prior to staining with 1% aqueous uranyl acetate and lead citrate (Leica). They were viewed on a Philips CM100 Compustage Transmission Electron Microscope with images collected using an AMT CCD camera (Deben) ...
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bioRxiv - Developmental Biology 2021Quote: ... RNA/DNA FISH) were acquired with a laser-scanning confocal microscope with a pinhole diameter of 1 Airy unit (Leica TCS SP5 or SP8 ...
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bioRxiv - Cell Biology 2022Quote: ... V5-tag-specific antibody was used at 1:100 dilution (Cat # R960-25, Invitrogen) (. Cells were imaged using a Leica DMi8 confocal microscope (63x ...
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Social Regulation of Egg Size Plasticity in the Honey Bee is Mediated by Cytoskeleton Organizer Rho1bioRxiv - Evolutionary Biology 2022Quote: ... The dehydrated samples were embedded in paraffin and then cut into 1 μm sections using a RM2235 microtome (Leica, Germany) that were gently deposited onto glass microscope slides ...
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bioRxiv - Immunology 2022Quote: ... sections were stained with hematoxylin for 1 minute followed by whole slide imaging using an Aperio ImageScope AT (Leica Biosystems) at 20X magnification ...
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bioRxiv - Cancer Biology 2022Quote: ... Heat-induced epitope retrieval was performed using citrate-based BOND Epitope Retrieval Solution 1 (Leica Biosystems, Buffalo Grove, IL, USA) for 10 min ...
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bioRxiv - Cell Biology 2020Quote: ... Ultrathin sections (50nm) were deposited onto formvar-coated copper grids (Plano) and postcontrasted using 1% uranyl acetate in water and ultrostain (Leica). TEM images were acquired on a JEM 1400plus (JEOL ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... IHC at a 1:50 dilution for 30 minutes on a Bond RX auto-stainer using the Bond Polymer Refine RED Kit (Leica) minus Hematoxylin.
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bioRxiv - Biophysics 2020Quote: ... deeply anesthetized fish larvae with immobilized hearts were embedded in 1% low-melting agarose for sustained confocal imaging (SP8-STED/FLIM/FCS, Leica) using a 20×/0.75 Objective (HC PL APO CS2) ...
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bioRxiv - Immunology 2021Quote: ... were cut and deposited onto formvar-coated grids (Plano) and again contrasted using 1 % uranyl acetate in water and Ultrostain (Leica). Images were acquired on a JEM 1400plus (JEOL).
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bioRxiv - Plant Biology 2021Quote: ... 1 μm thick sections were cut with a glass knife from each resin-embedded tissue block on an ultramicrotome (Leica EM UC6 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... the cells were washed with PBS containing 1% bovine serum albumin and the fluorescence (red and blue) was imaged under a fluorescence microscope (Leica). At least three fields of view were selected and analyzed using ImageJ software.
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bioRxiv - Biophysics 2023Quote: ... The collagen gel is stretched uniaxially in 1% step increments using a custom made stepper motor-driven cell stretching device (46) mounted on an epifluorescence microscope (Leica DMI6000 CS equipped with a 20x Leica HCX PL Fluotator objective ...
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bioRxiv - Immunology 2023Quote: ... The emission wavelength was tuned to 841 nm and the fluorescent signal was collected using a water immersion objective (25x, NA 1, Leica) and detected with external ...
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bioRxiv - Pathology 2023Quote: ... semi-thin sections measuring 1 µm were meticulously sliced from the resin blocks employing a Reichert Ultracut S microtome (Leica Microsystems Inc. ...
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bioRxiv - Neuroscience 2023Quote: ... 1 µm per z-step) of neurons expressing fluorescent membrane markers were captured using a 20×0.7 NA (Leica SP5) 20×0.75 NA (Leica Stellaris ...
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bioRxiv - Neuroscience 2023Quote: ... until the brains sank in the solution (1-3 days) and at which point 35um thick hypothalamic sections were obtained using a cryostat (Leica). Sections were first incubated for 1 hour in blocking buffer (1X PBS with 2% BSA and 0.1% tween-20) ...
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bioRxiv - Neuroscience 2022Quote: ... brains were rapidly removed and coronal slices (300 μm) containing the right AC were prepared in a cutting solution at 1 °C using a Vibratome (VT1200 S; Leica). The cutting solution ...
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bioRxiv - Molecular Biology 2022Quote: ... Adult animals were then immobilized with 1% sodium azide and GFP fluorescence was assessed using a DM6000B-Cs microscope (Leica) equipped with a DFC 365FX camera (Leica ...
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bioRxiv - Cell Biology 2022Quote: ... and stained with 0.1% toluidine blue to quantify the different germ cell types at 100x objective using a high-resolution light microscope (Leica DM6000 BD ...
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bioRxiv - Cancer Biology 2023Quote: ... The following antibodies were used at the indicated dilution and indicated antigen retrieval method: Ki67 (CST 12202, 1:1000, ER2 Leica), CD8a (eBioscience 14-0808-82 ...
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bioRxiv - Neuroscience 2024Quote: ... Nuclear DNA was stained using Hoechst 33258 (1:10,000, Pierce) Brain sections - Post fixed brains were sectioned via vibratome (Leica VT1200) at 100 µm ...
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bioRxiv - Microbiology 2024Quote: ... Quantifoil grids consisting of a 1.2/1.3 μm holey carbon film on 300 gold mesh were glow discharged at 15 mA for 1 minute with an EM ACE200 glow discharger (Leica). Just before vitrification ...
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bioRxiv - Immunology 2023Quote: ... images were acquired with z-stacks between 10-15 pm (z-steps: 1 pm) and 3D-reconstructed and displayed in ‘Maximum’-mode using the Leica Application Suite X software (Leica).
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bioRxiv - Microbiology 2022Quote: ... Cells were kept in serum- and phenol red-free MEM containing 1% glucose during the analysis (Leica Biosystems 6000B microscope). A total of 15 random snapshots (55-70 cells in total ...
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bioRxiv - Immunology 2022Quote: Imaging was performed on an inverted Leica TCS SP8 confocal microscope with white light laser and HyD photodetectors using an HC PL APO CS2 20 × /0.75 IMM objective (zoom factor of 1) (Leica Microsystems). Images were deconvolved using the LIGHTNING module in Leica Application Suite X (Leica Microsystems) ...
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bioRxiv - Plant Biology 2022Quote: ... Seedlings with the typical mdf-1 phenotype were transferred to a new plate to be observed under the fluorescent stereo microscope (Leica M165 FC Fluorescent stereo microscope equipped with a Leica DFC 420C camera ...
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bioRxiv - Cancer Biology 2023Quote: ... Heat-induced epitope antigen retrieval was performed at 100°C for 20 min in Bond Epitope Retrieval Solution 1 (Cat#AR9961, Leica) for GRPR or Bond Epitope Retrieval Solution 2 (1/100 ...
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bioRxiv - Cancer Biology 2023Quote: ... Slides were incubated in anti-GRPR antibody (SP4337P, Acris Antibodies) in Bond Primary Antibody Diluent (1/100, Cat# AR9352, Leica) overnight at 4°C and anti-Ecad (1/100 ...
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bioRxiv - Biochemistry 2023Quote: ... In the first channel, a 1-ml syringe tip (Braun, 9166017V) was used to connect the perfusion needle (Leica, 39471024) and the reference tube (Ismatec Reglo ...
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bioRxiv - Developmental Biology 2024Quote: ... and whole-mounted mouse kidney explants were performed using (1) Leica SP8-X confocal fluorescence imaging system (Leica Microsystems, Germany), in 1024 x 1024 pixels using 25X water ...
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bioRxiv - Neuroscience 2024Quote: Z-stacks of zebra finch SETBP1 and co-localization experiments in Area X were acquired with a SP8-1 confocal microscope (Leica). All microscope settings were kept constant for all conditions and slices ...
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bioRxiv - Neuroscience 2024Quote: ... Z-stack were taken at 1 μm intervals using a 40x objective lens (W CORR CS2, NA: 1.10, Leica, Germany) on the Stellaris8 microscope (Leica ...
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bioRxiv - Developmental Biology 2024Quote: ... Included tendon constructs were then frozen at -80°C in isopentane for 1 minute and 12µm cryosections were generated with a CM3050 S Cryostat (Leica Biosystems).
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bioRxiv - Microbiology 2024Quote: ... Washed cell pellets were resuspended in a total volume of 50 µl PBS and 10 µl of each sample was spotted onto 1 % agar pads and subsequently imaged using the Thunder imager 3D cell culture microscope (Leica). First ...
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bioRxiv - Physiology 2024Quote: ... and covered with #1 thickness cover glass (Fisherbrand, cat#12-545-F) using a histological automaton (Leica Biosystem, cat#ST5020). Negative controls were treated with 1% BSA in PBS without the primary or secondary antibodies.
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bioRxiv - Neuroscience 2024Quote: ... 20x tiled GFAP and EM48 fluorescent images were acquired with a 16 μm z-dimension and 1 μm step size on a confocal microscope (Stellaris, Leica). Blinded images were opened in FIJI/ImageJ and condensed using Z project feature at maximum intensity ...
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bioRxiv - Neuroscience 2024Quote: ... 20x tiled EM48 fluorescent images from the striatum were acquired with a 16 μm z-dimension and 1 μm step size on a confocal microscope (Stellaris, Leica). Images were blinded ...
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bioRxiv - Neuroscience 2024Quote: ... and then a 1-mm-thick coronal slice containing the entire SCN was prepared using a vibratome (VT 1200S, Leica). Following this ...
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bioRxiv - Bioengineering 2024Quote: The specimens were immersed in an external cryoprotectant solution (1-hexadecene) and gently placed into HPF sample carriers (depth: 0.2 mm, Type A, Leica 16770141). The cover (Type B ...
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bioRxiv - Cell Biology 2024Quote: ... ZO-1 and Claudin-5 were acquired using a Leica SP-8 HCX PL APO CS at 63× magnification (Leica) or a DMI-8 TIRF microscope at 20× magnification (Leica Microsystems) ...
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bioRxiv - Cancer Biology 2024Quote: ... Briefly, MHC-II (clone V1030, dilution 1:500; Biomeda, Foster City, CA, USA) was revealed using Novolink Polymer (Leica Biosystems). Slides were then decolored using ethanol and the previous antibody was stripped using a β-mercaptoethanol/SDS solution in a waterbath preheated at 56°C ...
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bioRxiv - Cancer Biology 2024Quote: ... The following antibody was used: anti CD31 (CST, 3528, clone 89C2, 1:1600, antigen retrieval Leica ER1 AR9961 for 30min).
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bioRxiv - Cell Biology 2020Quote: ... Samples were mounted laterally in 1% agarose and imaged with a 10x objective lens on a SP5 confocal microscope (Leica Microsystems).
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bioRxiv - Immunology 2021Quote: Grids were screened for cells with ASC/caspase-1 speck by light microscopy using a cryo-FM microscope (Leica EM Cryo CLEM, Leica Microsystems), equipped with a cryo-stage ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... Animals were observed under blue-light-cut illumination by a macroscope with an apochromat objective lens (1 ×) (Z16 APO, Leica, Germany) and recorded in an H264 compressed movie6 ...
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bioRxiv - Bioengineering 2021Quote: ... The experimental setup consisted of (1) our microfluidic device installed on a Leica inverted microscope (DMIL LED Inverted Routine Fluorescence Microscope, Leica, Germany) and imaged using a colour camera (MC170 HD ...
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bioRxiv - Plant Biology 2021Quote: ... tumefaciens clones carrying the appropriate BiFC plasmids using a 1 mL needleless syringe and imaged two days post-infiltration with a Leica TCS SMD confocal microscope (Leica Microsystems) using the preset settings for YFP (Ex ...
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bioRxiv - Neuroscience 2020Quote: ... Mice were anesthetized with a mixture of oxygen (1 L/min) and isoflurane 3% (Baxter AG, Vienna, Austria) and placed in a stereotactic frame (Angle One; Leica, Germany). The skin was shaved ...
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bioRxiv - Cell Biology 2020Quote: ... Interaction between swiprosin-1 and Rab21 in cells was detected using confocal microscopy (Leica TCS SP5, 63×1.4 Apo oil objective) and the number of PLA spots per 1000 µm3 was quantified using IMARIS software.