Labshake search
Citations for Leica :
1151 - 1200 of 1921 citations for 6 propan 2 yl 1 3 5 triazine 2 4 diamine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... After subsequent freeze substitution (using acetone, containing 0.25% osmium tetroxide, 0.2% uranyl acetate, 0.05% ruthenium red, and 5% water) (Leica AFS2), cells were embedded in Epon812 substitute resin (Fluka) ...
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bioRxiv - Cell Biology 2022Quote: A minimum of 5 fields per cover slip were imaged using a SP-8 Lightning Confocal Microscope (Leica) or CSU-W1 Spinning-Disk Confocal Microscope (Nikon) ...
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bioRxiv - Molecular Biology 2023Quote: ... cardiac specimens were cut into 5-µm-thick longitudinal sections using a freezing sliding microtome (Leica 2000R, Germany) (15723985) ...
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bioRxiv - Developmental Biology 2023Quote: ... and tissues were sectioned at 5 micron thickness using a Leica microtome for downstream applications (Leica Biosystems, RM2155).
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bioRxiv - Immunology 2023Quote: ... the fetuses were embedded in paraffin and 5 μm thick sections were cut with a microtome (Leica Biosystems). Morphometric analyzes were performed on E18 and E21 slides ...
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bioRxiv - Physiology 2023Quote: ... Sciatic nerves were then embedded in paraffin and 5 µm sections were obtained with a microtome (Leica Biosystems). Paraffin sections were melted at 60 °C for 30 min ...
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bioRxiv - Biochemistry 2023Quote: ... tissue blocs were sectioned to 5 μm thickness on a rotary microtome (RM 2125RT Leica Microsystems, Wetzlar, Germany). Gallya’s silver staining was performed following the manufacturer’s protocol (Morphisto ...
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bioRxiv - Cell Biology 2024Quote: ... 10 μm (Arabidopsis and barley roots) or 5 μm (barley leaves) of cryosections cut by cryostat (Leica CM1850) were attached to selected polysine adhesion slides (PolysineTM adhesion microscope slides Cat.# 48382-117 ...
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bioRxiv - Cell Biology 2023Quote: ... Experiments were performed at 37°C with 5% CO2 using a total internal reflection fluorescence (TIRF) microscope (Leica AM TIRF inverted microscope ...
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bioRxiv - Cell Biology 2023Quote: ... The wafer was then coated with a 5 nm carbon layer using an EM ACE 600 (Leica Microsystems) to ensure conductivity ...
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Neutralizing gut-derived lipopolysaccharide as a novel therapeutic strategy for severe leptospirosisbioRxiv - Microbiology 2024Quote: ... The sample was then embedded in optimal cutting temperature compound (O.C.T., Tissue-Tek) and cryo-sectioned into 5 μm longitudinal sections (Leica). Slides were equilibrated in hybridization buffer (0.9 M NaCl ...
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bioRxiv - Pathology 2024Quote: Intimal hyperplasia: Paraffin cross sections (5 μm thick) were cut at equally spaced intervals using a microtome (Leica) and then stained (Hematoxylin and Eosin ...
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bioRxiv - Genomics 2024Quote: ... DAPI was applied for 5 min for nuclear counterstaining before visualization using confocal microscopy (Leica TCS SP5 MP). PLA was performed usin the DuolinkII fluorescence system (Olink Bioscience ...
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bioRxiv - Microbiology 2024Quote: ... Brains embedded in 5% agarose were cut into 500 μm-thick sections using a vibratome (Leica VT1220, Biosystems). Brain sections were clarified using Rapid Clear® CS mounting solution (SunJin Lab ...
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bioRxiv - Neuroscience 2021Quote: ... 40 μm brain slices from animals perfused with 4% paraformaldehyde (in PBS, pH-7.4) were cut with a cryostat (Leica CM 1800; Leica Canada) and used for immunohistochemistry (IHC) ...
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bioRxiv - Cancer Biology 2019Quote: ... and images were collected through 4 fluorescence channels and stitched together using microscope software (LAS X, Leica or cellSens, Olympus). Image analysis was performed using the FIJI software 50 ...
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bioRxiv - Neuroscience 2019Quote: Brains of mice (n = 4 per group) perfused with 4% PFA/PBS were serially cut into 50 μm-thick coronal sections using vibratome (VT1200S, Leica), and the sections from one cohort of mice (a littermate pair of wild-type and Bdnf+/- mice ...
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bioRxiv - Biochemistry 2021Quote: ... 3 μl of MutS-nucleotide sample were adsorbed onto glow-discharged grids and blotted for 1 second at 80% humidity at 4°C and flash frozen in liquid ethane using an EM GP plunge freezer (Leica). All cryo-EM data was collected at the The Netherlands Center for Electron Nanoscopy (NeCEN) ...
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bioRxiv - Neuroscience 2020Quote: ... followed by immersion in 30% sucrose at 4°C sucrose and then embedded in an OCT block for cryosectioning at 35μm thickness (Leica, Germany) and used for histological studies.
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bioRxiv - Evolutionary Biology 2021Quote: ... 300µm-thick acute coronal brain sections were cut after embedding in 4% low melt-agar using a vibratome (Leica, #VT1000S) under RNase-free conditions ...
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bioRxiv - Neuroscience 2020Quote: ... the other half was placed first in 4% paraformaldehyde for 48 hr and then in a 30% sucrose solution for 48 hr at 4°C and cut into 30-µm-thick sections with a freezing microtome (Leica). For Extended Data Fig ...
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bioRxiv - Neuroscience 2020Quote: ... 2.5% glutaraldehyde and 4% paraformaldehyde in buffer solution) removed and coronally cut into 350 µm thick sections using a Vibratome (Leica VT1000 S ...
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bioRxiv - Neuroscience 2019Quote: ... Brains were post-fixed overnight in 4% paraformaldehyde and then cryoprotected in a 30% sucrose and 0.1M PBS solution prior to sectioning (Leica CM3050S, Leica Biosystems). Three sets of immediately adjacent sections (40 μm ...
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bioRxiv - Neuroscience 2020Quote: ... Slices were then cryoprotected for 2h in 20% sucrose in PBS at 4°C and sectioned at 20 µm or 40-45 µm thickness with a sliding microtome (Leica). The tissues were then processed in function of the target immunofluorescence as described below.
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bioRxiv - Neuroscience 2020Quote: ... the slices were embedded in 4% low melting agarose and cut in 70µm thick sections in a vibrating microtome (Leica, VT1000S). Sections around the tip of the recording electrode were collected and cryoprotected in 10% and 20% sucrose solutions in PB ...
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bioRxiv - Physiology 2021Quote: ... then individually embedded in paraffin blocks and sectioned in serial sagittal sections (4 μm) using a rotary microtome (Leica, Germany). Tissue sections were permanently mounted ...
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Sequential dynein effectors regulate axonal autophagosome motility in a maturation-dependent pathwaybioRxiv - Cell Biology 2020Quote: ... with an Apochromat 63 x 1.4 NA oil-immersion objective and a charge-coupled device camera (ORCA-R2; Hamamatsu Photonics) using LAS-AF software (Leica). Color-coded time projections were generated in ImageJ using a publicly available plugin (K ...
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bioRxiv - Plant Biology 2022Quote: ... Approximately 20 µl of the protoplast suspension were pipetted to 200 µL high osmolarity solution (Supplementary Table 4) in a perfusion chamber mounted on the stage of an inverted microscope (DMi8, Leica). Protoplasts were allowed to settle down for 5 minutes ...
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bioRxiv - Molecular Biology 2022Quote: HEK293 TauRDP301S-CFP/YFP and HEK293 TauRDP301S-CFP cells treated with Tau condensates were fixed with 4% PFA in PBS prior to confocal FLIM on a Stellaris 8 FALCON (Leica) equipped with a 100x objective ...
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bioRxiv - Genomics 2022Quote: ... each liver sample was exhaustively sectioned into 4 μm-thick sections by a fully automated rotary microtome (Leica RM2255, Germany). Each of these sections was stained with hematoxylin and eosin and mounted ...
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bioRxiv - Microbiology 2022Quote: ... the eyes were cut into 4 μm sections and stained with H&E (BBC Biochemical) using an autostainer (ST5010, Leica). Images were obtained using an Olympus BX51 microscope (Olympus ...
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bioRxiv - Developmental Biology 2020Quote: ... images were taken as a time series on an xy plane across 6 z planes at an interval of 4 s using a 10× objective with an NA of 0.4 and an optical zoom of 4 on an SP5 inverted confocal microscope mounted with a resonant scanner (Leica Microsystems). The z project across time was then obtained as a time series.
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bioRxiv - Neuroscience 2020Quote: ... One hemisphere was post-fixed by incubation for 48 h in 4% paraformaldehyde (PFA) and cut into 35 μm sections with a vibratome (Leica) for histological analyses (brains used for RNAscope were perfused with both PBS and 4% PFA prior post-fixation and cut in 10 μm sections with a cryostat) ...
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bioRxiv - Cell Biology 2021Quote: ... brains fixed in 4% paraformaldehyde (PFA) were embedded in paraffin and sectioned 5µm thick using a microtome system (Leica RM2165). Immunohistochemistry was performed on brain sections by permeabilizing them with TBS-0.5% Triton-X (TBS-Tx ...
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bioRxiv - Cancer Biology 2020Quote: Paraffin tissue-blocks were serially sectioned with slices thickness of 4 µm on a microtome (Leica Byosystem, Buffalo Grove, IL) and allowed to dry at room temperature (RT ...
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bioRxiv - Neuroscience 2020Quote: ... brains were post-fixed in 4% PFA overnight and coronally sectioned at 75 µm on a Vibratome (Leica VT1000 S). All slices were then mounted on Superfrost Plus slides using Fluoromount-G mounting medium (Southern Biotech ...
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bioRxiv - Neuroscience 2019Quote: ... then transcardially perfused with 10% sucrose in phosphate buffered saline (PBS) followed by 4% paraformaldehyde in PBS for approximately 90 s each using a Perfusion Two system (Leica). The brain was then removed and postfixed in 4% paraformaldehyde in PBS overnight at 4°C before being moved to a 30% sucrose solution in PBS for cryoprotection ...
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bioRxiv - Developmental Biology 2019Quote: ... The samples were embedded in gelatin/ albumin with 4% of glutaraldehyde and sectioned (20 μm) on a VT1000 S vibrating blade microtome (Leica). Sections were mounted in Fluoromount Aqueous Monting Medium (Sigma).
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bioRxiv - Neuroscience 2019Quote: ... The OE were embedded in 4% low melting point agarose blocks and sectioned (30 µm) using a Vibratome (VT 1200; Leica). The sections were blocked in 1% blocking solution (10 min at RT ...
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bioRxiv - Developmental Biology 2019Quote: ... EBs were fixed in 4% paraformaldehyde for 7-8 hours at 4°C followed by equilibration in 20% sucrose solution overnight and embedded in tissue freezing medium (Leica). The embedded tissues were then cryo-sectioned and collected on Superfrost slides (Fisher Scientific) ...
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bioRxiv - Neuroscience 2019Quote: ... Brains were cut coronally into 15-μm sections and mounted in 4 series using a cryostat (Leica CM1950, Nussloch, Germany) to obtain 16 matching sections containing the DR (−7.3 to −8.7 mm from bregma ...
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bioRxiv - Plant Biology 2019Quote: ... Nodules were embedded in 4% agarose and 50-100 μM sections were made with a vibratome® 1000 plus (Leica). Sections were immersed in a staining solution (2.5% 5-Bromo-4-chloro-3-indoxyl-beta-D-glucuronic (X-Gluc) ...
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bioRxiv - Neuroscience 2019Quote: ... 2.5 mM KCl, 25 mM glucose, 1.25 mM NaH2PO4, 4 mM MgCl2, 0.5 mM CaCl2, and 24 mM NaHCO3, Leica VT1200 vibratome). The slices were incubated at 32 °C for 60 minutes after sectioning and were then stored at room temperature until they were used ...
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bioRxiv - Neuroscience 2021Quote: ... Striatum containing hNSCs (equivalent to +1.4 to +0.14 mm from Bregma) (Franklin and Paxinos, 2007) was cut at 60 μm using a vibratome (Leica Microsystems). After pre-embed IHC of the striatum using diaminobenzidine (DAB ...
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bioRxiv - Developmental Biology 2021Quote: ... Fixed heads were embedded in 4% low gelling agarose and 200 µm sections were obtained using a vibratome (Leica, VT1200S). Sections were post-fixed with 1% osmium tetroxide in water ...
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Sequential dynein effectors regulate axonal autophagosome motility in a maturation-dependent pathwaybioRxiv - Cell Biology 2020Quote: ... with an Apochromat 63 x 1.4 NA oil-immersion objective and a charge-coupled device camera (ORCA-R2; Hamamatsu Photonics) using LAS-AF software (Leica). Puncta were counted manually using ImageJ.
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bioRxiv - Neuroscience 2021Quote: ... followed by immersion in 30% sucrose at 4°C sucrose and then embedded in an OCT block for cryosectioning at 35μm thickness (Leica, Germany) and used for histological studies.
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bioRxiv - Neuroscience 2020Quote: ... Brains were post-fixed overnight at 4°C and cut into 30 μm-thick free-floating sections with a vibrating microtome (Leica). Brain sections were kept at −20°C in a solution containing 300 mL.L−1 ethylene glycol ...
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bioRxiv - Neuroscience 2022Quote: ... The paraffin embedded sections were then sliced into 4 μm-thick slices on a Leica RM2016 Microtome (Leica, Shanghai, China) for immunohistochemistry staining and apoptotic cells labeling ...
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bioRxiv - Neuroscience 2023Quote: ... and brains were postfixed in 4% paraformaldehyde and preserved with 30% sucrose before sectioning at 40-µm on a freezing-sliding microtome (SM2010R, Leica). Sections were incubated overnight with indicated primary antibodies ...