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Citations for Leica :
1151 - 1200 of 1603 citations for 3 2 5 Dioxoimidazolidin 4 yl propanoic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Brains not used for two-photon imaging were postfixed overnight in 4% paraformaldehyde in PBS on a shaker at 4°C and cut into 50 µm coronal (S1 injections) or parasagittal (SNc injections) sections on a vibrating microtome (Leica, VT-1000S). Sections were collected sequentially into 6 tubes containing cryoprotectant ...
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bioRxiv - Neuroscience 2023Quote: ... the samples were dehydrated using an ascending ethanol series (30%-100%) at 4℃ and then critically dried using a low-speed critical point dryer (Leica EM CPD300). Subsequently ...
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bioRxiv - Neuroscience 2023Quote: ... Brains were postfixed overnight in 4% paraformaldehyde in PBS on a shaker at 4°C and cut into 50 μm coronal sections on a vibrating microtome (Leica, VT-1000S). Sections were collected sequentially into 6 tubes containing cryoprotectant ...
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bioRxiv - Developmental Biology 2023Quote: ... The oocytes were fixed in 4% PFA/PBS for 30 min and observed using the Leica M205FA stereomicroscope (Leica Microsystems, Wetzlar, Germany) or FV3000 Confocal Microscope.
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bioRxiv - Bioengineering 2024Quote: ... The fixed constructs were embedded in low-melting point agarose 4% w/v in pre-warmed PBS and sliced to a thickness of 300 μm with a vibratome (Leica Biosystems, VT1000S) filled with PBS 1x ...
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bioRxiv - Neuroscience 2023Quote: ... Images of cultured ependymocytes co-treated with Sir-Actin and calcein AM were captured at 10-s intervals for 12 minutes while that of co-treated with Sir-Actin and fluo-4 AM were captured at 10-s intervals for 6 minutes using confocal scanning microscope (DMi8, Leica, Wetzlar, Germany). The intensity of F-actin area from all Sir-Actin positive cells in a field of recording were included in the statistics ...
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bioRxiv - Neuroscience 2023Quote: ... The fluorescence signals of Fluo-4 were monitored at 10-s intervals for 6 minutes by confocal scanning microscope (DMi8, Leica, Wetzlar, Germany). As previously described 46 ...
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bioRxiv - Bioengineering 2024Quote: ... and fetal pig kidneys were fixed with 4% paraformaldehyde (FUJIFILM Wako Pure Chemical Corporation) and sectioned with a cryostat (Leica Microsystems GmbH) to prepare frozen sections ...
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bioRxiv - Cell Biology 2023Quote: ... organoids were cryoprotected in 30% sucrose/PBS on a rotor shaker overnight at 4°C and sectioned at 20 µm on a cryostat (Leica 1850 UV). Sections were permeabilized in 0,3% Triton X-100/PBS ...
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bioRxiv - Neuroscience 2024Quote: ... the brains were embedded in 4% oxidized agarose as previously described (Ragan et al., 2012) to limit artifacts during sectioning on a vibratome (Leica VT1000 S). For 20 μm cryotome sectioning ...
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bioRxiv - Immunology 2024Quote: ... The brains were quickly dissected and fixed in 4% paraformaldehyde (PFA) overnight and then cut into 30 µm sections using a vibrating microtome (Leica, VTS1200, Germany). For immunostaining ...
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bioRxiv - Immunology 2024Quote: ... and tonsil sections of 4 µm-thick were deparaffinized at 70°C in 100% Clearene solvent (Leica Biosystems, Buffalo Grove, IL, USA) solution and washed in ethanol (EtOH ...
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bioRxiv - Neuroscience 2024Quote: Hemibrains were drop-fixed in 4% paraformaldehyde for two days and sectioned at 40 mm on a vibratome (Leica, Buffalo Grove, IL). Sections were collected in a 1 in 4 series and stored in PBS at 4°C ...
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bioRxiv - Immunology 2024Quote: ... Brains were placed in 4 °C s-ACSF and sectioned with a vibrotome at 350–400 μm thick (Leica VT1200S; Leica, Nusslock, Germany). Collected slices were then incubated at 32 °C for 1 h in i-ACSF (124 mM/L NaCl ...
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bioRxiv - Microbiology 2024Quote: ... sucrose in PBS overnight at 4°C and embedded in OCT compound (Agar Scientific) and cryosectioned (10 µm thick, Leica CM1850 Cryostat). Sections were dried overnight onto Thermo Scientific SuperFrost Plus Adhesion slides ...
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bioRxiv - Developmental Biology 2024Quote: ... The tissues were then embedded in paraffin wax and cut into 4-μm sections with a microtome (RM 2165; Leica Microsystems GmbH). Sections were stained with haematoxylin and hemoglobin (H&E) ...
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bioRxiv - Molecular Biology 2021Quote: ... embedded in OCT tissue freezing medium and 5 μm cross-sectioned on a Cryostat (Leica CM3050 S, Leica Biosystems, USA). Cross-sections were placed in 1x TBS for 10 minutes ...
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bioRxiv - Molecular Biology 2021Quote: ... embedded in OCT tissue freezing medium and 5 μm cross-sectioned on a Cryostat (Leica CM3050 S, Leica Biosystems, USA). Cross-sections were placed in 1x TBS for 10 minutes ...
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bioRxiv - Bioengineering 2020Quote: ... Sections were placed on the ice block to dry for 5 minutes prior to LCM using a laser microdissection microscope (Leica).
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bioRxiv - Developmental Biology 2021Quote: ... Stained embryos were embedded in Technovit 7100 resin (Kulzer Technique) and 5 µm sections were obtained using an RM2245 microtome (Leica). Sections were counterstained with Mayer’s hematoxylin solution and images were captured by BX61 microscope (Olympus ...
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bioRxiv - Biophysics 2020Quote: ... These polyps were then embedded in 5 % Agar and sectioned into 200 μm slices using a vibratome (Leica VT1200 S). The sections were then stained with 1 % Osmium tetroxide (Sigma) ...
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bioRxiv - Plant Biology 2021Quote: ... The resulting samples were cut to 5 µm by using a Reichert-Jung 820H Histostat rotary microtome (Leica, Wetzlar, Germany) and Drukker VC186200 diamond knives (Cuijk ...
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bioRxiv - Neuroscience 2020Quote: ... brains were embedded in a 5% low-gelling agarose gel (in PBS) and 50µm coronal slices were cut using a vibroslicer (Leica VT1000S). Slices were then stored in PBS-Azide (0.02-0.05% ...
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bioRxiv - Cell Biology 2021Quote: ... INS-1E cells were incubated with HO-PI for 15 min (5 mg/ml) and observed using a confocal laser-scanning microscope (Leica SP5 ...
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bioRxiv - Cell Biology 2022Quote: ... Plasmid DNA (10 µl of 5 µg/µl solution) was injected into each testis using glass capillaries under a stereomicroscope (M165C; Leica). Testes were held between a pair of tweezers-type electrodes (CUY21 ...
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bioRxiv - Neuroscience 2022Quote: ... Near-horizontal middle entorhinal cortical (bregma –6.5 mm to –5.1 mm) slices of thickness of 350 μm were obtained using a vibrating blade microtome (Leica Vibratome) while the brain was submerged in oxygenated ice-cold cutting solution ...
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bioRxiv - Microbiology 2021Quote: ... imaging the colony and crystal formation at regular intervals at 5× magnification with a Leica MZ7.5 stereomicroscope (Leica, United Kingdom), using an Infinity 2 Lumenera microscope camera (Teledyne Technologies Inc. ...
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bioRxiv - Cancer Biology 2021Quote: ... 5 μm sections were taken for H&E staining using standard methods and imaged using a DMI 6000B microscope (Leica). To examine tdTomato expression in sections ...
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bioRxiv - Biochemistry 2022Quote: ... serial 5 μm thick sections of adult scallop’s eye were obtained using a Leica CM1950 freezing microtome (Leica, Wetzlar, Germany) for Raman spectroscopy testing (alpha300R ...
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bioRxiv - Immunology 2023Quote: ... with a stage top incubator to maintain 37 °C and 5% CO2 or on a Leica TCS SP8 Laser Scanning Confocal (Leica) fluorescence microscope with an incubator to maintain 37 °C and 5% CO2.
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bioRxiv - Biophysics 2023Quote: ... A volume of 3ul of the sample was applied onto glow-discharged (Leica EM ACE200, 30 s at 5 mA) grids (Quantifoil R1.2/1.3 200 mesh Cu grids ...
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bioRxiv - Developmental Biology 2023Quote: ... and 18.5 counting images were acquired using Leica Application Suite X (LAS X) on a Leica DMI8 fluorescent microscope from Leica Microscopy ...
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bioRxiv - Cell Biology 2023Quote: ... followed by determining the fluorescence lifetime of GFP in different cells cultured in a humidified chamber with 5% CO2 at 37 °C using a STELLARIS 8 FALCON (Leica) systems equipped with HyD X and HyD SMD detectors and an HC PL APO CS2 63x/1.40 OIL objective (Leica) ...
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bioRxiv - Cell Biology 2023Quote: ... the tibiae were embedded in methyl methacrylate (MMA) and 5 µm sections cut with a tungsten steel knife on a motorised rotary microtome (Leica). Sections were stained either with Goldner’s Trichrome to analyse osteoid and lipid droplets or counterstained with calcein blue to quantify the amount of bone formed between the injected alazarin red-S and calcein ...
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bioRxiv - Bioengineering 2022Quote: PTFE surfaces were sputter-coated with platinum to develop a 5 nm thick conductive layer on the surface using an EM ACE600 sputter coater (Leica). Images were acquired using a Quanta 450 scanning electron microscope (FEI company).
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bioRxiv - Biophysics 2024Quote: ... were evenly positioned on a ring installed 5 cm above the sample positioned at the focal plane of the objective lens (OBJ; 100×/1.44, Leica, Germany), as shown in Fig ...
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bioRxiv - Molecular Biology 2024Quote: ... paraffin-embedded tissue was sectioned into approximate 0.5cm3 cubes and then cut into 5-μm slices by a LEICARM2235 Slice Machine (Leica, Germany). The samples were stained with haematoxylin/eosin (H/E ...
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bioRxiv - Neuroscience 2024Quote: ... 87 mM NaCl and 25 mM D-glucose) gassed with 95% O2/5% CO2 to give a pH of 7.4 using the VT-1200 Vibratome (Leica, Germany). Slices were then allowed to recover for at least an hour in a chamber filled with ACSF ...
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bioRxiv - Developmental Biology 2024Quote: ... Tissue visualization and image collection was performed using a Leica Stellaris 5 Confocal microscope and LAS X software (Leica Microsystems). All images were acquired using 40X (HC PL APO CS2 ...
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bioRxiv - Developmental Biology 2023Quote: ... ganglionic eminences explant cultures were placed in a humidified and thermoregulated chamber maintained at 37 °C and 5% CO2 on the stage of a spinning-disk microscope (Leica), equipped with an Orca Flash 4.0 Camera ...
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bioRxiv - Cell Biology 2023Quote: ... HeLa cells expressing OMM-Cepia3 and ER-mit RspA-splitFAST were mounted into an open-topped chamber in mKRB supplemented with TFCoral (5 µM) and imaged by a Thunder Imager 3D Cell Culture (Leica), equipped with a LED8 illumination system ...
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bioRxiv - Neuroscience 2023Quote: ... and immobilized in 5% methyl cellulose for imaging with a M165 FC stereo microscope and LAS Software V4.21 (Leica Microsystems). Total body length measurements and eye area were quantified using Fiji/ImageJ software (63) ...
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bioRxiv - Plant Biology 2023Quote: ... cell walls were labelled with propidium iodide (5 µg/mL) and imaged using a 20x PLAN APO multi-immersion objective (Leica), with λexc = 488 nm and λem = 551-651 nm ...
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bioRxiv - Pathology 2023Quote: ... 5 μm thick sections were taken from the paraffin blocks using a rotary microtome (Leica RM2155, Leica Microsystems, Wetzlar, Germany) and stained with hematoxylin-eosin (HE) ...
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bioRxiv - Plant Biology 2024Quote: ... The cells were then washed 3 times for 5 min with PBS and covered with PBS before imaging them with a TCS SP8 confocal laser-scanning microscope (Leica). Excitation/emission settings were 488 nm/498-524 nm HyD1 SMD hybrid detector for CAH4 ...
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bioRxiv - Cancer Biology 2022Quote: Hematoxylin and Eosin (H&E) staining was performed on 5 μm thick slices using an automated system (Leica Autostainer, ST5010) with the protocol as described in Supplemental Table 1 ...
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bioRxiv - Cell Biology 2022Quote: ... Retinal cups were embedded in 5% agar moulds and immediately underwent sectioning on a fully automated vibratome (Leica VT1200 S). Sections were ‘Chased’ by incubation with 0.6 µM SNAP-Cell 647-SiR (New England Biolabs (S9102S ...
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bioRxiv - Neuroscience 2022Quote: ... Horizontal slices containing the substantia nigra were cut at 222 μm in Krebs buffer bubbled with 95/5% O2/CO2 using a vibrating microtome (Leica). Slices were allowed to recover at 30°C in vials with 95/5% O2/CO2 Krebs with MK801 (10 μM ...
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Serotonin, Etonogestrel and breathing activity in murine Congenital Central Hypoventilation SyndromebioRxiv - Physiology 2022Quote: ... and c-FOS/5-HT immunolabeled cells were visually counted by an investigator blinded to samples using a light microscope (Leica DM2000 ...
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bioRxiv - Bioengineering 2023Quote: ... Immunostained cells and tissues were imaged on the Evos Revolve widefield microscope using a 20x objective or Stellaris 5 confocal microscope (Leica) with a 10x or 25x water-immersion objective to capture data in DAPI ...