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Citations for Leica :
1101 - 1150 of 1487 citations for Mouse IL 12 p40 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... were acquired over a ∼30 mm x ∼3 mm rectangular area by using the tile scan feature of the LAS X software (Leica Microsystems, Buffalo Grove, IL). This involved frame by frame acquisition (36 × 5 = 180 frames ...
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bioRxiv - Neuroscience 2023Quote: ... The extracted brain was blocked coronally with a brain matrix (Zivic Instruments; Pittsburg, PA) and acute coronal slices (300 µm) were cut on a vibratome (VT1000S, Leica Microsystems; Buffalo Grove, IL) through the striatum in ice-cold ...
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bioRxiv - Neuroscience 2024Quote: Immuno-stained anti-rabbit Iba-1 brain sections were imaged at a 40x oil objective using a confocal imaging system (Leica Microsystems, Deerfield, IL, USA) to further investigate microglia structure and morphology ...
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bioRxiv - Neuroscience 2024Quote: ... All histological images were acquired on a Nikon fluorescence microscope (Eclipse Ni-U, Melville, NY, USA) and confocal imaging system (Leica Microsystems, Deerfield, IL, USA). Areas of interest were the somatosensory cortex and thalamus regions of the brain ...
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bioRxiv - Cancer Biology 2021Quote: ... mouse anti-MUC2 (1:50, Leica, NCL-MUC-2), mouse anti-CK17 (1:10 ...
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bioRxiv - Immunology 2019Quote: ... A mouse monoclonal anti-CD3ε (clone LN10, Leica Biosystems) was used to stain for pig T cells ...
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bioRxiv - Biochemistry 2023Quote: ... mouse brain sections and coronal mouse kidney sections were cryosectioned at 10 µm thickness using a CM1860 UV Cryostat (Leica Biosystems, Wetzlar, Germany) and thaw mounted onto washed ITO slides ...
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bioRxiv - Neuroscience 2021Quote: ... One of the hemispheres was cut into 12 series of 25µm-thick sagittal sections using a sliding microtome (Leica SM2010R, Wetzlar, Germany). The sections were stored in a cryoprotection solution (25% ethylene glycol and 25% glycerin in 0.1M PB ...
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bioRxiv - Neuroscience 2020Quote: ... brains were incubated in 10% and then 30% sucrose in PBS for 12-24 hours at 4 °C before being cut into 50 µm sections by a freezing-sliding microtome (Leica SM 2101R). Sections from V1 were mounted on gelatin-coated slides and cover-slipped with Prolong Diamond Antifade Mounting Media (P36965 ...
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bioRxiv - Neuroscience 2020Quote: Quantification of mitochondria transfer events was obtained from randomized n≥ 12 brain ROIs acquired using a confocal microscope (Leica TCS SP5 Microscope) and data are expressed as % ± SEM of fGFP-/MitoDsRed+ particles over total fGFP-/MitoDsRed+ particles found in GFAP+ astrocytes ...
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bioRxiv - Neuroscience 2020Quote: ... Spinal cord sections and longitudinal sciatic nerve sections were cut at 12 μm and DRGs at 10 um thickness using a cryostat (Leica Biosystems, CM3050S). Sciatic nerve and DRG sections were mounted on Superfrost+ microscope slides (Fisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... brains were incubated in 10% and then 30% sucrose in PBS for 12-24 hours at 4 °C before being cut into 50 µm sections by a freezing-sliding microtome (Leica SM 2101R). Sections with LGN and V1 were then mounted on gelatin-coated slides and cover-slipped with mounting media (Prolong Diamond Antifade Mounting Media ...
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bioRxiv - Molecular Biology 2022Quote: ... Green siliques were harvested 12 d after flowering from 4-6 week old plants and opened using fine needles under a dissecting microscope (Leica MZ9.5, Germany). Images of opened siliques were captured using a Leica DFC290 (Germany ...
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bioRxiv - Neuroscience 2020Quote: ... containing dorsal hippocampus (from 3, 6, 9, 12, 15, 19, and 24 month-old) were cut from PFA-fixed hemispheres (Leica vibratome VT1000P) and stored in 0.1M phosphate-buffered saline (PBS ...
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bioRxiv - Developmental Biology 2022Quote: ... Each embryo was mounted in the embedding media in a cryosection mold placed on dry ice and 12-μm sagittal cryosections were obtained (Leica CM3050 S).
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bioRxiv - Developmental Biology 2019Quote: ... and cryosectioned at 20 μm thickness onto either Superfrost Plus glass slides (Fisher 12-550-15) (for staining optimization) or PEN membrane slides (Fisher/Leica No. 11505158) (for LCM) ...
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bioRxiv - Biophysics 2020Quote: ... stage of growth were imaged every 10 minutes for 12 hours in a customized incubator (37°C, 5% CO2, and 95% humidity) on a confocal microscope (Leica, TCL SP8), with acquisition of fluorescent and bright-field image z-stacks (Fig ...
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bioRxiv - Developmental Biology 2022Quote: ... Processed limbs were embedded in OCT (optimal cutting temperature) (Agar Scientific, Stansted, UK) diluted with 50% sucrose and cut (12 μm thickness) using a cryostat (NX70, Leica Biosystems, UK). Then ...
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bioRxiv - Molecular Biology 2023Quote: ... Tissue was stored in −80 °C and sectioned from an OCT block at 12 µm thickness and mounted on Leica PPS-membrane Laser Microdissection (LMD) slides (Leica, Cat# 11505268). A rapid stain protocol23 prepared slides for LMD ...
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bioRxiv - Neuroscience 2023Quote: ... embedded in OCT mounting medium and cut into 12 μm thick transverse sections with a cryostat (Leica CM1950; Leica; Milton Keynes, UK). These were mounted in Prolong-Glass anti-fade medium with NucBlue (P36981 ...
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bioRxiv - Neuroscience 2023Quote: ... embedded in OCT mounting medium and cut into 12 μm thick transverse sections with a cryostat (Leica CM1950; Leica; Milton Keynes, UK). These were mounted in Prolong-Glass anti-fade medium with NucBlue (P36981 ...
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bioRxiv - Neuroscience 2023Quote: ... Images of cultured ependymocytes co-treated with calcein AM and Sir-Actin were captured at 10-s intervals for 12 minutes using confocal scanning microscope (DMi8, Leica, Wetzlar, Germany). During the imaging ...
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bioRxiv - Neuroscience 2023Quote: ... The cell volume of cultured ependymocytes were visualized by calcein signals at 10-s intervals for 12 minutes using confocal scanning microscope (DMi8, Leica, Wetzlar, Germany). The cell volume from all calcein positive cells in a field of recording selected of every culture dish used in experiment were included in the statistics ...
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bioRxiv - Neuroscience 2024Quote: ... 30μm serial sagittal sections were collected from Cx3cr1GFP/+ animals in a tempero-medial manner (1 in 12 series) using a vibratome (Leica VT 1200S) and kept as free-floating sections in ice-cold PBS ...
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bioRxiv - Developmental Biology 2021Quote: ... Electroporation efficiency and extent of overexpression were evaluated at the time of embryo collection by detecting GFP and RFP on a stereo dissecting microscope under epifluorescent illumination (MZFLIII-TS, Leica Microsystems, Buffalo Grove, IL, USA).
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bioRxiv - Cell Biology 2019Quote: ... GFPdgn direct fluorescence (green) and the stained F-actin (red) were visualized and imaged using a Leica TCS SP8 confocal microscope (Leica Microsystems Inc., Buffalo Grove, IL).
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bioRxiv - Cancer Biology 2019Quote: ... These cross-sections of the lungs were stained with H&E and scanned by an Aperio ScanScope® CS System (Leica Biosystems, Buffalo Grove, IL). The metastatic burden was calculated as the percentage of metastasis/lung area and the number of foci/lung area ...
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bioRxiv - Neuroscience 2020Quote: ... the rats were sacrificed via transcardiac perfusion with 4% paraformaldehyde (PFA) using the Perfusion Two™ Automated Pressure Perfusion System (Leica Biosystems, Buffalo Grove, IL) (n = 12 ...
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bioRxiv - Physiology 2021Quote: ... All sections of both patellae and femora were stained with Safranin-O/Fast Green and imaged at 20x magnification using a slide imaging system (Aperio, Leica 122 Biosystems, Buffalo Grove, IL). Two blinded raters scored each section using the Osteoarthritis Research Society International (OARSI ...
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bioRxiv - Cell Biology 2021Quote: ... INS-1E cells were incubated with HO-PI for 15 min (5 mg/ml) and observed using a confocal laser-scanning microscope (Leica SP5; Leica Microsystems, Buffalo Grove, IL, USA).
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bioRxiv - Bioengineering 2020Quote: ... and polymerized at 60 °C for 48 h.[73] Ultrathin sections of ∼70 nm were produced in an ultra-microtome (Leica Biosystems, Buffalo Grove, IL, USA), transferred to copper grids (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2022Quote: ... The z-stack images of the whole tissue were acquired with a 5 mm step size in a Leica TCS SP5 confocal microscope (Leica Microsystems, Inc., Buffalo Grove, IL). Images were segmented and volumetrically reconstructed with Imaris software (Bitplane ...
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bioRxiv - Biochemistry 2022Quote: ... The grid is immediately plunged into liquid ethane at its freezing point (−183 °C) using an automatic plunger (Leica, EM GP, Buffalo Grove, IL, USA). The vitrified specimens are analyzed using a FEI Tecnai 12 G2 TEM at 120 kV with a Gatan cryo-holder maintained at −180 °C ...
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bioRxiv - Molecular Biology 2022Quote: ... All images were taken no longer than 2 hrs after gavage using the wide-field feature of a LEICA DM6 confocal microscope (Leica Microsystems Inc., Buffalo Grove, IL).
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bioRxiv - Cell Biology 2021Quote: Interdigitation analysis was performed on cryosections stained with Hematoxylin and Eosin as previously described [74] and imaged using an Aperio ScanScope (Leica Biosystems, Buffalo Grove, IL, USA) and analyzed using Aperio ImageScope v12 software (Leica Biosystems ...
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bioRxiv - Plant Biology 2021Quote: Leaves were cut into small pieces and placed on glass slides and observed with a Leica TCS SP5 confocal laser scanning microscope system (Leica Micro-systems, Bannockburn, IL, USA). GFP fluorescence was detected with excitation at 488nm and emission capture at 500–530 nm ...
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bioRxiv - Molecular Biology 2020Quote: ... the individual primordial oocyte was collected from 2-3 adjacent tissue sections to ensure that one full cell was included (Leica Microsystems, Buffalo Grove, IL, USA).
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bioRxiv - Cancer Biology 2021Quote: ... The double staining was done sequentially on a Leica Bond-IIITM instrument using the Bond Polymer Refine DAB Detection System (Leica Microsystems DS9800, Buffalo Grove, IL) and Refine Red Detection System (Leica Microsystems DS9390) ...
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bioRxiv - Developmental Biology 2022Quote: ... pilot experiments confirmed single cell dissociation with this protocol by visual inspection using the DIC feature of a LEICA DM6 confocal microscope (Leica Microsystems Inc., Buffalo Grove, IL). All groups of dissociated cells had over 80% viability and were diluted to a final concentration of 3,500 cells/ul in 0.04% BSA/HBSS for cDNA library preparation ...
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bioRxiv - Cell Biology 2022Quote: Plates seeded with NRVMs were incubated in a stagetop incubator at 37°C with 5% CO2 (TokaiHit, Shizuoka, Japan) on a DMi8 wide-field fluorescence microscope (Leica Microsystems, Inc., Buffalo Grove, IL). Spontaneous contractions were recorded real-time under phase contrast microscopy ...
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bioRxiv - Cell Biology 2022Quote: ... Spontaneous contractions of cardiomyocytes were recorded real-time under phase contrast microscopy using a DMi8 wide-field fluorescence microscope (Leica Microsystems, Inc., Buffalo Grove, IL).
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bioRxiv - Molecular Biology 2021Quote: ... High-resolution Stimulated Emission Depletion (STED) images were acquired on Leica TCS SP5 II STED-CW laser scanning confocal microscope (Leica Microsystems, Inc., Buffalo Grove, IL). Emission depletion was accomplished with 592 nm STED laser ...
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Oxytocin receptor activation does not mediate associative fear deficits in a Williams Syndrome modelbioRxiv - Animal Behavior and Cognition 2021Quote: ... Brains were frozen on crushed dry ice and then stored at −80 °C until sliced into 20 μm coronal sections in a cryostat (Leica Biosystems 1850, Buffalo Grove, IL) at −16 to −18 °C ...
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bioRxiv - Neuroscience 2021Quote: ... Brains were cut down the midline and each half vibratome sectioned into 40 µm sagittal slices using a Leica Vibratome (VT1000S; Leica Biosystems Inc., Buffalo Grove, IL) and stored in PBS at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were cut at 10 μm using a cryostat and sections containing the striatum and SNc/ventral tegmental area (VTA) were collected on Leica PET FrameSlides (Leica Microsystems, Buffalo Grove, IL, USA). Slides were stored at −80 °C until staining.
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bioRxiv - Neuroscience 2020Quote: ... which shows expression in uninjured axons.25 All immunostains were performed using a Leica Bond III automated immunostainer with a diaminobenzidine chromogen detection system (DS9800, Leica Biosystems, Buffalo Grove, IL, USA).
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bioRxiv - Developmental Biology 2021Quote: ... The heads of treated embryos were stained with Hoechst 33342 Dye and imaged on a stereo dissection microscope under epifluorescent illumination (MZFLIII-TS, Leica Microsystems, Buffalo Grove, IL, USA).
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bioRxiv - Developmental Biology 2021Quote: ... The heads of quail at HH27 were stained with Hoechst 33342 Dye (see below) and imaged on a stereo dissection microscope under epifluorescent illumination (MZFLIII-TS, Leica Microsystems, Buffalo Grove, IL, USA).
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bioRxiv - Neuroscience 2021Quote: ... GAD65 perisomatic puncta as well as levels of PV present in hM4DGi-mCherry and mCherry-expressing cells in the prelimbic region of the PFC were estimated from analysis of 10x images acquired on a Leica confocal microscope (Leica Microsystems, Buffalo Grove, IL, USA) of sections stained for either PV and mCherry or GAD65 and MAP2 ...
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bioRxiv - Neuroscience 2020Quote: ... All stained slides were scanned at 20x magnification into digital images using the Aperio scanner system (Aperio AT2 - High Volume, Digital whole slide scanning scanner, Leica Biosystems Inc., Richmond, IL, USA) for analysis.