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Citations for Leica :
1101 - 1150 of 1247 citations for 3' Chloro 3 3 chloro 5 fluorophenyl 4' fluoropropiophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2021Quote: ... The lung tissue sections of 3-5 micron were stained with H&E stain and were microscopically analyzed using microscope (Make: Leica DM1000 LED; Camera: Leica MC170HD). The images were taken with 10X objective lens (Magnification X 100 ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were mounted on an aluminum stub with a silver lacquer and sputtercoated with a 5 nm platinum layer using EM ACE600 (Leica Microsystems). Acquisitions were performed using a GeminiSEM 500 (Zeiss).
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bioRxiv - Cell Biology 2022Quote: ... lensectomy and sclerectomy were performed and retinas embedded in 5% agar and 150 µm sections made using a fully automated VT1200S vibrating blade microtome (Leica Biosystems). Sections were cultured in retinal media (DMEM extra (Sigma ...
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bioRxiv - Microbiology 2022Quote: ... at 5 µm in thickness with a tungsten carbide blade (SL-T30, SECTION LAB Co. Ltd) using a cryostat system (Leica CM1850). The undecalcified sections attached to the adhesive film were immerged in 100% ethanol for 30 sec to remove the compound and air-dried ...
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bioRxiv - Neuroscience 2022Quote: ... Their brains were quickly dissected out and stuck on a 5 mm agar stage before being mounted on a vibratome (Leica VT1200S) equipped with a sapphire blade ...
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bioRxiv - Neuroscience 2022Quote: ... and Homo (N=5 males) Gcg-Cre rats from the Janvier colony were cut into 50 μm coronal slices using a cryostat (Leica CM3050S). The cNTS was then microdissected using biopsy punches (Integra Miltex ...
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bioRxiv - Physiology 2022Quote: ... then incubated with serum-free protein block for 5 minutes (Novocastra Protein Block Protein Block Serum Free, Leica Biosysems, cat# RE7102), followed by incubation with rabbit anti-FABP4 polyclonal antibody (adipose ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and 5% H2O) under liquid nitrogen and placed in an automatic freeze substitution system (Leica AFS2, Leica Microsystems, precooled to −90 ℃) for 24 h ...
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bioRxiv - Neuroscience 2024Quote: ... the lumbar spinal cord from L3-5 was mounted on an agarose mold and the dorsal halve was micro dissected on a vibratome (Leica Microsystems). Next ...
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bioRxiv - Cell Biology 2024Quote: ... Paraffin-embedded WAT was sliced into 5-μm sections using a ThermoScientific HM 355S Automatic Microtome and mounted on Surgipath X-tra Microscope Slides (Leica Biosystems).
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bioRxiv - Neuroscience 2024Quote: Mice were anesthetized using vaporized isoflurane (5% for induction, 1.5% for maintenance) and placed in a stereotactic frame (Leica, Angle 2). Opthalmic ointment was applied and rectal temperature and respiration rate were monitored throughout surgery ...
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bioRxiv - Evolutionary Biology 2023Quote: ... LEGE 07298 and Phormidesmis priestleyi ULC 007 stained with either calcein or 5-CF were acquired with an inverted confocal laser scanning microscope (CLSM; Leica SP8) using a HC PL APO x63/1.40 oil objective ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were mounted on an aluminum stub with a silver lacquer and sputtercoated with a 5 nm platinum layer using EM ACE600 (Leica Microsystems). Acquisitions were performed using a GeminiSEM 500 (Zeiss).
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Apical expansion of calvarial osteoblasts and suture patency is dependent on graded fibronectin cuesbioRxiv - Developmental Biology 2023Quote: ... Confocal images were captured at 63X oil immersion objective on Leica STELLARIS 5 (Leica; HC PL APO 63x/1.40 OIL CS2) using Application suite X software ...
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bioRxiv - Synthetic Biology 2023Quote: ... The frozen samples were transferred to 1% Osmium tetroxide and 0.1% uranylacetate in acetone and freeze substituted using a Leica EM AFS2 freeze substitution processor at – 90°C with a slow warm up of 5°C per hour up to 0°C (Leica Microsystems). As osmium becomes increasingly reactive at higher temperatures we removed the samples from the AFS2 at 0°C to preserve the “crispiness” of the staining ...
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bioRxiv - Cell Biology 2023Quote: ... Slices were imaged with a laser scanning confocal Stellaris 5 or TCS SP8 gSTED equipped with a white light laser and a 405-diode ultraviolet laser (Leica Microscope). Image acquisition was done using the LAS-X software in accordance with the Nyquist sampling sequential mode by exciting the fluorophores and collecting their signal with hybrid detectors (HyDs).
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bioRxiv - Cell Biology 2023Quote: ... All confocal images were acquired using the Leica TCS SP8 or Stellaris 5 confocal microscope with the Leica LAS X software (Leica Microsystems). Following objectives were used with the Leica TCS SP8 ...
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bioRxiv - Cancer Biology 2023Quote: ... at a final concentration of 5 µg/mL for 20 minutes at 37 °C prior to live cell imaging by epifluorescence (Leica DMI6000B). Cells were counted as DAPI-positive if the nucleus showed bright staining and as apoptotic if there was characteristic nuclear fragmentation ...
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bioRxiv - Cancer Biology 2022Quote: ... was incubated at 100 degrees Celsius for 20 minutes (for anti-Cytokeratin 5, Bond Epitope Retrieval 1, pH6.0 (Leica Biosystems, AR9961) was used instead) ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were placed in a 37°C chamber incubator supplemented with 5% CO2 mounted on Leica TCS SP8X microscope system (Leica Microsystems).
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bioRxiv - Microbiology 2023Quote: ... as described above, the cells were incubated (37°C, 5% CO2) inside the Mica Microhub system using the internal incubator (Leica microsystems). Videos were analysed using the complementary software (Leica microsystems).
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bioRxiv - Neuroscience 2023Quote: ... The STG was then illuminated for 5-7 mins using a Texas red filter set (560 ± 40 nm wavelength; Leica Microsystems). Complete photoinactivation was confirmed when the LP membrane potential reached 0 mV ...
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bioRxiv - Plant Biology 2023Quote: Lengths of root RAM and EZ regions and root hairs in 5-d-old seedlings grown on vertical MS medium without sucrose were measured under a Leica microscope (Leica DM5000). The data were analyzed using the ImageJ software (http://rsb.info ...
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bioRxiv - Neuroscience 2023Quote: ... the brain was briefly submerged in ice cold cut solution bubbled with carbogen (95% oxygen, 5% CO2) and then sectioned into 300μm sagittal sections (Leica VT1200S vibratome) in bubbled ice-cold cut solution ...
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bioRxiv - Neuroscience 2024Quote: ... Brains were then frozen and coronally sectioned at a thickness of 25 um (1:5 series) using a LeicaCM1900 cryostat (Leica Microsystems). Sections were mounted on gelatin-coated slides and coverslipped with VECTASHIELD containing DAPI (catalog no ...
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bioRxiv - Neuroscience 2024Quote: ... Brains were then frozen and coronally sectioned at a thickness of 25 mm (1:5 series) using a LeicaCM1900 cryostat (Leica Microsystems). Following immunohistochemistry for cFos ...
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bioRxiv - Neuroscience 2024Quote: ... The lumbar spinal cord (L2-5) was sectioned transversely (300-350 µm) with dorsal roots attached using a vibrating microtome (Leica Microsystems). Slices were next transferred to an artificial cerebrospinal fluid (ACSF) ...
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bioRxiv - Biophysics 2024Quote: ... The removed brain was washed thrice with PBS (5 min each, shaking in a shaker) and subsequently sectioned into 100 µm thick sections using a vibratome (Leica, VT1000S). The resulting brain sections were subjected to label-free CARS and SHG imaging.
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bioRxiv - Neuroscience 2024Quote: ... The STG was then illuminated for 5 – 7 min using a Texas red filter set (560 ± 40 nm wavelength; Leica Microsystems). Complete photoinactivation was confirmed when the LP membrane potential reached 0 mV.
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bioRxiv - Plant Biology 2024Quote: ... mounted in H2O on a slide and imaged with a 63x glycerol immersion objective on a Leica TCS SP8 microscope or a Leica Stellaris 5 microscope (Leica Microsystems). On the SP8 we imaged using an Argon laser at 20% power ...
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bioRxiv - Microbiology 2024Quote: ... at 17°C and 63.5 bar and the sample was sputter coated with a 5 nm thick layer of Au/Pd (80/20) using the Leica ACE600 (Leica Microsystems). Samples were mapped by SEM using an Aquilos 2 dual-beam cryo-focused ion beam-scanning electron microscope (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... The STG was then illuminated for 5-7 min using a Texas red filter set (560 ± 40 nm wavelength; Leica Microsystems). Complete photoinactivation was confirmed when the LP membrane potential reached ∼0 mV (8 ...
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bioRxiv - Neuroscience 2024Quote: ... the brain was briefly submerged in ice cold cut solution bubbled with carbogen (95% oxygen, 5% CO2) and then sectioned into 300μm sagittal sections (Leica VT1200S vibratome) in bubbled ice-cold cut solution ...
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bioRxiv - Molecular Biology 2024Quote: ... were acquired with a Leica SP5 laser scanning confocal microscope or with Leica Stellaris 5 confocal microscope and LAS AF Lite software (Leica Microsystems). The images were analysed with ImageJ.
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bioRxiv - Biophysics 2024Quote: ... we deposited approximately 5 nm of carbon to the nanopipettes using thermal evaporation (CED 030, BalTec) with double-stranded carbon thread (Leica Microsystems). We imaged the nanopipettes by scanning electron microscopy (LEO 1530 VP ...
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bioRxiv - Developmental Biology 2024Quote: ... before being embedded in 5% low-melting agarose and sectioned into 100-400 μm-thick slices using a Vibratome (Leica, VT1200). Slices were blocked and permeabilized using PBST supplemented with 5% donkey serum and 0.3% Triton X at room temperature for 30 minutes ...
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Microfluidic Formation of Ultrathin, Handleable Collagen Sheets Exhibiting Toe-heel Tensile BehaviorbioRxiv - Bioengineering 2024Quote: The morphology of collagen sheets was imaged under wet condition were using a light sheet/confocal microscope (model Leica Stellaris 5), equipped with a water immersion lens (40× magnification ...
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bioRxiv - Cancer Biology 2024Quote: ... Processed tissues were embedded in paraffin and sectioned at room temperature using a manual rotary microtome (5 um sections; Leica RM2235). Frozen tongues were sectioned using a cryostat (10 um sections ...
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bioRxiv - Developmental Biology 2024Quote: ... Long bone samples were embedded in paraffin after dehydration and 5-7 μm-thick paraffin sections were cut with a microtome (RM2255, Leica Biosystems). Sections were stained with von Kossa ...
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bioRxiv - Developmental Biology 2024Quote: ... The ovaries were dissected in 5% glycerol (Carl Roth) and immediately analyzed using a Leica DMI6000b epifluorescence inverted microscope (Leica Microsystems), equipped with GFP ...
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bioRxiv - Developmental Biology 2024Quote: ... Stacks with a z-space of 0.4 μm were captured with a confocal microscope (Stellaris 5, Leica Microsystems GmbH, Wetzlar, Germany). The previous segmentation model trained on Hoechst labelled images was refined in Cellpose to create models for nuclei and GM130 labeled images.42 Digital masks of nuclei and Golgi bodies were created with Cellpose ...
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bioRxiv - Cell Biology 2020Quote: ... neural crest populations were captured at 4 days post-fertilation (dpf) using a Leica Sp8 and analyzed with the Leica Application Suite X (Leica Microsystems, Buffalo Grove, IL) software for image capturing ...
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bioRxiv - Neuroscience 2022Quote: ... containing the primary somatosensory cortex (S1) were cut in ice-cold (4 °C) NMDG ACSF using a Leica VT1200s vibratome (Leica Biosystems, Buffalo Grove, IL) and transferred to warm (33 °C ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The animals were given cold shock for 2 minutes at -4°C and then photographed along with a reference scale under a stereo zoom microscope (Leica DFC 290, Leica Microsystems GmbH, Wetzlar, Germany) with a mounted digital camera ...
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bioRxiv - Neuroscience 2022Quote: ... Mice were head-fixed in a 1.5 x 4-inch polycarbonate tube under a custom-built fluorescence macroscope consisting of back-to-back 50 mm objective lens (Leica, 0.63x and 1x magnification), separated by a 495nm dichroic mirror (Semrock Inc ...
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bioRxiv - Molecular Biology 2020Quote: Frozen coronal ovine hypothalamic blocks (n=4 per group) for in-situ hybridization were cut into 16µm sections using a cryostat (CM3050s Leica Microsystems, Ltd., Milton Keynes, UK), and thaw mounted onto poly-l-lysine coated slides (VWR International ...
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bioRxiv - Cell Biology 2021Quote: ... stained with DAPI (4’,6-diamidino-2-phenylindole) for visualisation of the nuclei and analysed by confocal microscopy using an inverted microscope (Leica DMI6000, objective lens 100×).
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bioRxiv - Neuroscience 2021Quote: ... and sacrificed via transcardiac perfusion with 10% sucrose (isotonic at 9.25% in distilled water; prewash) and 4% paraformaldehyde (PFA; fixative) using the Perfusion Two™ Automated Pressure Perfusion System (Leica Biosystems, Buffalo Grove, IL) (n = 12 ...
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bioRxiv - Neuroscience 2022Quote: ... embedded them in 4% w/v agar/PBS and prepared 40 µm thick slices using a vibrating blade microtome (VT1200S, Leica Microsystems, Buffalo Grove, IL). The slices were post-fixed in 4% PFA/PBS for 30 min at room temperature (RT ...
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bioRxiv - Neuroscience 2022Quote: ... 0.4 mM L-ascorbic acid and the pH was adjusted to 7.4) and then sliced on a vibratome (Leica, VT1200 S, Deerfield, IL, USA). Brain slices (300 μm thick ...