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Citations for Leica :
1101 - 1150 of 1420 citations for 2 Amino 6 chloro 9 3 5 di O p toluoyl beta D 2 deoxyribofurnanosyl purine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... Serial sections of 5 μm thickness were cut and stained using hematoxylin and eosin (H&E; Leica) to evaluate spongiform changes ...
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bioRxiv - Neuroscience 2022Quote: Images (n=5 per brain area per mouse) were acquired using a SP8 confocal microscope (LEICA, Germany) with a 63x oil immersion objective (PL APO CS2 ...
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bioRxiv - Biophysics 2024Quote: ... After blotting for 4-5 s in a Leica EM GP2 plunge freezer (Leica Microsystems, Wetzlar, Germany) with the chamber set to 5°C and 95% humidity ...
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bioRxiv - Physiology 2024Quote: ... The slides were air-dried for 5 minutes before loading onto a Bond Rx instrument (Leica Biosystems). Slides were prepared using the frozen slide delay prior to pre-treatments using Epitope Retrieval Solution 2 (Leica Biosystems ...
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bioRxiv - Cancer Biology 2024Quote: ... Tumor blocks were cut into 5 µm sections using a Leica RM2135 microtome (Leica Biosystems, Wetzlar, Germany). Slides were dried overnight at 40°C ...
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Short-range interactions between fibrocytes and CD8+ T cells in COPD bronchial inflammatory responsebioRxiv - Pathology 2023Quote: ... at 37°C and with 5% CO2 on an inverted DMi8 stand microscope (Leica, Microsystems, Wetzlar, Germany) equipped with a Flash 4.0 sCMOS camera (Hamamatsu ...
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bioRxiv - Molecular Biology 2023Quote: ... and cross-sectioned to a thickness of 5 µm using a Leica RM2025 microtome (Leica Microsystems, Germany). The obtained histological sections were initially subjected to topographic staining using the hematoxylin-eosin (H/E ...
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bioRxiv - Immunology 2023Quote: ... Counterstaining was performed with hematoxylin for 5 min and slides were finally mounted with Micromount (Leica Biosystems).
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bioRxiv - Neuroscience 2022Quote: ... Brains were embedded in 5% agarose and sliced along the coronal axis at 50 µM (Leica, VT1200S). Sections were mounted onto glass slides (VWR 48311-703 ...
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bioRxiv - Immunology 2022Quote: ... Compound (23-730-571, Fisher Healthcare) and cryosectioned at 5 μm thickness using a cryostat (Leica CM1850) and adhered to positively charged glass slides (12-550-15 ...
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bioRxiv - Plant Biology 2023Quote: ... Transverse and longitudinal sections (5 μm in thickness) were obtained with an automatic rotating microtome (RM2155; Leica) equipped with a disposable glass knife ...
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bioRxiv - Cancer Biology 2024Quote: ... Confocal images were collected using a TCS-SP 5 spectrophotometric multiphoton laser scanning confocal microscope (Leica Microsystems) collecting 0.2 - 1 µm optical sections.
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bioRxiv - Cell Biology 2024Quote: ... Experiments were performed at 37°C with 5% CO2 using a total internal reflection fluorescence microscope (Leica AM TIRF inverted microscope ...
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bioRxiv - Microbiology 2024Quote: ... Live-cell imaging was conducted using an automated inverted confocal laser scanning microscope (Leica Stellaris 5 Confocal). mCherry fluorescence was excited at 580 nm with a laser set to 5% power ...
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bioRxiv - Neuroscience 2024Quote: Vitrified samples were imaged at -195°C utilizing the STELLARIS 5 Cryo Confocal Light Microscope from Leica Microsystems ...
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bioRxiv - Microbiology 2024Quote: ... The stained dishes were visualized with an inverted confocal laser scanning microscope (CLSM) (Leica Stellaris 5, DMi8) using a 20× objective ...
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bioRxiv - Neuroscience 2024Quote: ... The slides were air-dried for 5 min before loading onto a Bond Rx instrument (Leica Biosystems). Slides were prepared using the frozen slide delay prior to pre-treatments using Epitope Retrieval Solution 2 (Cat No ...
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bioRxiv - Neuroscience 2024Quote: ... Brains were embedded in 5% agarose and sliced along the coronal axis at 50 µm (Leica, VT1200S).
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bioRxiv - Neuroscience 2024Quote: ... and Z-stacks with 0.12 µm spacing were obtained on the Stellaris 5 confocal microscope (Leica Microsystems) with a 63x or 100x oil objective ...
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bioRxiv - Cell Biology 2024Quote: ... z-stack images of CDMs and dCDMs were collected with a Leica TCS SP 5 (Leica Microsystems) combined with MPM in which the NIR femtosecond laser beam was a tunable compact mode-locked titanium:sapphire laser (Chameleon Compact OPO-Vis ...
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bioRxiv - Neuroscience 2021Quote: ... brains were then transferred to a 30 % sucrose solution for at least 3 days and coronal sections (200 µm) containing hippocampus were obtained using a vibratome (Leica vt 1000S). Sections were collected into 24-wells plate in sucrose 6% for one day stored at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... The embedded tissues were sectioned to 3 μm-thick sections and stained with Haematoxylin-Erythrosin-Saffron (HES) before visualizing under a Leica DMRBE light microscope (Leica Microsystems, Germany). The stained sections were scanned with a Hamamatzu NanoZomer S60 ...
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bioRxiv - Plant Biology 2022Quote: ... 1 cm segments from the root tip were embedded in 3% agarose and were sectioned at 150-200 μM using a vibratome (Leica VT1000 S). The sections were stained in Clearsee buffer (Ursache et al ...
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Genome modularization reveals overlapped gene topology is necessary for efficient viral reproductionbioRxiv - Synthetic Biology 2020Quote: ... blotting for 3-seconds at 4 °C with 90% humidity and plunging into liquid ethane using a Lecia EM GP device (Leica Microsystems, Germany). Cryo-EM data were collected on a Talos Arctica transmission electron microscope operated at 200 kV acceleration voltage using EPU automated data collection software (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... endogenous peroxidase activity was blocked by incubation with 3% peroxide block for 20 minutes (BOND Polymer Refine Detection System, Leica, Germany; DS9800). The sections were blocked with 10% normal goat serum (Vector ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... At least 3 pictures/section were acquired at 40x and 63x magnification by DMI600 wide field fluorescence microscope (Leica microsystems, Wetzlar, Germany). Hoechst signal (cell nucleus ...
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bioRxiv - Neuroscience 2021Quote: ... The STG or stn near its entrance to the STG was then illuminated for 3-7 min using a Texas red filter set (560 ± 40 nm wavelength; Leica, Wetzlar, Germany). Complete photoinactivation of LP was confirmed when the membrane potential reached 0 mV ...
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bioRxiv - Neuroscience 2024Quote: Paraffin-embedded fixed postmortem human brain tissues (from 34 AD patients and 30 NCs) were cut into 3 µm sections using a Leica microtome (Leica Microsystems, Germany) (46 ...
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bioRxiv - Developmental Biology 2024Quote: ... Brains were embedded in 3% low-melting point (LMP) agarose and sectioned coronally at 100 μm using a vibratome (Leica, model VT1200). Free-floating sections were incubated in block solution (2% donkey serum ...
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bioRxiv - Neuroscience 2023Quote: ... Larvae were placed into a petri dish filled with HL-3 on ice and imaged (camera: Leica DFC365 FX, microscope: Leica MZ10 F). Brains were stained against HRP ...
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bioRxiv - Plant Biology 2022Quote: ... The roots were washed in water and one cm segments from the root tip were embedded in 3% agarose and made sections of 150-200 μM using a vibratome (Leica VT1000 S). Confocal Laser Scanning microscopy was performed on a Zeiss LSM700 confocal with the 20X objective ...
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bioRxiv - Neuroscience 2022Quote: ... The brains were rinsed in 0.1 M phosphate buffer (PB; 3 × 10 min) and 50-70 μm-thick coronal sections were obtained with a vibrating blade microtome (Leica VT1000 S).
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bioRxiv - Neuroscience 2023Quote: ... were rinsed in PBS and embedded in 3% agarose containing 1% Tween 20 and sectioned at 300 µm in the frontal plane with a vibratome (Leica VT 1200S). Under a stereomicroscope (Olympus SZX7) ...
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bioRxiv - Cancer Biology 2024Quote: All stained sections except for those from experiments involving Lepob mice (Fig. 3) were scanned at 20x magnification using a Leica Aperio AT2 slide scanner (Leica Microsystems, UK) prior to subsequent analysis of scanned slides as described below.
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bioRxiv - Biophysics 2024Quote: ... grids were applied with 3 μL 10 nm diameter BSA Gold Tracer (Aurion, The Netherlands) and single-side blotted using the EMGP plunger (Leica, Wezlar, Germany). Subsequent imaging of vitrified grids was performed on a Titan Krios microscope (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... Larvae subjected to TBI (as described above) at 3 dpf were anesthetized at 7 dpf with MS-222 and imaged under a fluorescent dissecting scope (Leica M165 FC). GFP+ puncta were counted from a lateral view (blinded).
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bioRxiv - Neuroscience 2024Quote: ... coli) and transferred onto 3% (w/v) agarose pads on a glass slide for confocal microscopy study (TCS SPE, Leica, Wetzlar, Germany). The excitation wavelength was set at 488 nm (Jex ∼488 nm) ...
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bioRxiv - Neuroscience 2024Quote: The paraffin-embedded fixed postmortem human brain tissues (from 34 AD patients and 31 NC subjects) were cut into 3 µm sections using a Leica microtome (Leica Microsystems, Germany). Hematoxylin and eosin (H&E ...
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bioRxiv - Neuroscience 2024Quote: ... The immunostaining on each slice (3 sections per slice) was imaged using the Leica TCS SPE-II laser scanning confocal microscope (Leica, Wetzlar, Germany) and averaged per rat.
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bioRxiv - Biochemistry 2024Quote: ... 3 µL of each sample were plunged into liquid ethane with a Leica EM GP automatic plunge freezer (Leica Microsystems, Wetzlar, Germany). The plunging chamber was set to have the following conditions ...
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bioRxiv - Cell Biology 2024Quote: ... adjusted pH 7.25–7.3 with 10 N NaOH to set Na 154 mM) using Leica VT1200 S fully automated vibrating-blade microtome (Leica, Wetzlar, Germany). Sections were kept at 37 °C for 20 min and then allowed to return to room temperature for the duration of the experiment ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... A series of time laps pictures was taken (Leica M50, 5 MP HD Microscope Camera Leica MC170 HD) every 10s for 24h ...
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bioRxiv - Cancer Biology 2022Quote: ... The specimen frozen blocks were serially sectioned longitudinally at respectively 5 μm thickness using Cryostat microtome (Leica CM1950). Sections were used for H&E and immunofluorescence staining ...
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bioRxiv - Molecular Biology 2023Quote: ... cardiac specimens were cut into 5-µm-thick longitudinal sections using a freezing sliding microtome (Leica 2000R, Germany) (15723985) ...
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bioRxiv - Cell Biology 2022Quote: A minimum of 5 fields per cover slip were imaged using a SP-8 Lightning Confocal Microscope (Leica) or CSU-W1 Spinning-Disk Confocal Microscope (Nikon) ...
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Neutralizing gut-derived lipopolysaccharide as a novel therapeutic strategy for severe leptospirosisbioRxiv - Microbiology 2024Quote: ... The sample was then embedded in optimal cutting temperature compound (O.C.T., Tissue-Tek) and cryo-sectioned into 5 μm longitudinal sections (Leica). Slides were equilibrated in hybridization buffer (0.9 M NaCl ...
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bioRxiv - Pathology 2024Quote: Intimal hyperplasia: Paraffin cross sections (5 μm thick) were cut at equally spaced intervals using a microtome (Leica) and then stained (Hematoxylin and Eosin ...
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bioRxiv - Genomics 2024Quote: ... DAPI was applied for 5 min for nuclear counterstaining before visualization using confocal microscopy (Leica TCS SP5 MP). PLA was performed usin the DuolinkII fluorescence system (Olink Bioscience ...
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bioRxiv - Microbiology 2024Quote: ... Brains embedded in 5% agarose were cut into 500 μm-thick sections using a vibratome (Leica VT1220, Biosystems). Brain sections were clarified using Rapid Clear® CS mounting solution (SunJin Lab ...
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bioRxiv - Cell Biology 2023Quote: ... The wafer was then coated with a 5 nm carbon layer using an EM ACE 600 (Leica Microsystems) to ensure conductivity ...