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Citations for Leica :
1051 - 1100 of 2150 citations for 7 chloro 1 2 diethylamino ethyl 5 2 fluorophenyl 1 3 dihydro 2H benzo 1 4 diazepin 2 one monohydrochloride since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... The sections were incubated with CD8 mouse monoclonal antibody (clone 4B11, 1:150, 0.2 µg IgG2b/ml) from Novocastra (Leica Microsystems, Newcastle Upon Tyne, UK) for 45 minutes ...
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bioRxiv - Molecular Biology 2021Quote: ... the thecal plates of the dinoflagellates were stained with the fluorescent dye Calcofluor white M2R at a final concentration of 10–20 mg mL−1 (Fritz & Triemer, 1985) and examined under epifluorescence (lamp 50W) using a Leica DM IRB (Leica Microsystemps GMbH, Wetzlar, Germany) inverted microscope ...
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bioRxiv - Cancer Biology 2022Quote: Tissue sections were incubated for 1 hour in primary antibodies and detected using the BOND Polymer Refine Detection System (DS9800; Leica Biosystems, Buffalo Grove, IL) in accordance with the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... The following antibodies were used: to detect muscle fibre types we used mouse anti- myosin heavy chain (slow) (Leica, clone WB-MHCs; 1:200); to delimit the fibres we used rabbit anti-collagen type VI (Abcam ...
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bioRxiv - Immunology 2024Quote: ... Sections were then stained for Ly6G (clone 1A8) at a previously optimized dilution (1/60000) for 30 min at room temperature with the autostainer Bond Rx (Leica Microsystems GmbH, Wetzlar, Germany). The IHC sections were visualised with 3,3’Diaminobenzidine (DAB) ...
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bioRxiv - Cancer Biology 2023Quote: 4 µm FFPE tissue sections were baked for 1 hour at 62°C in vertical slide orientation with subsequent prestaining of deparaffinization (Leica, Bond Dewax Solution, AR9222) and 35 minutes of Bond ER2 (Leica ...
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bioRxiv - Molecular Biology 2023Quote: ... Larvae were then positioned and oriented laterally to acquire two z-stacks of 30 optical sections (ΔZi, Zi-1 = 683.46μm) of the gastrointestinal tract using a 4X objective (Leica HC PL FLUOTAR 4X/0.13 DRY) at 10 msec exposures ...
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bioRxiv - Cancer Biology 2023Quote: Tissues sections were incubated for 1 h in primary antibodies and detected using the BOND Polymer Refine Detection System (DS9800; Leica Biosystems, Buffalo Grove, IL) in accordance with the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... Lewy bodies were counted on sections from the described regions following immunohistochemistry for alpha-synuclein (Leica, cat# ncl-l-asyn, 1:100-400 dilution). Of note ...
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bioRxiv - Immunology 2023Quote: ... Frozen brains were then embedded in Epredia™ M-1 Embedding Matrix (Thermo Ficher Scientific) and cut in coronal sections using a cryostat (Leica CM3050S, Mannheim, Germany), and mounted on slides for either fluorescence immunocytochemistry (IF ...
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bioRxiv - Plant Biology 2024Quote: ... Semi-thin sections at 1 µm were prepared using the resin-embedded buds using a glass knife on an EM UC6 ultramicrotome (Leica Microsystems GmbH, Wetzlar, Germany). Sections were mounted on glass slides ...
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bioRxiv - Molecular Biology 2024Quote: ... For scoring (graphs Figure 1) the percentage of cells expressing the chromatin markers were scored manually under an epi-fluorescence microscope (Leica DM6000 Leica microsystems, Germany).
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bioRxiv - Neuroscience 2024Quote: Immuno-stained anti-rabbit Iba-1 brain sections were imaged at a 40x oil objective using a confocal imaging system (Leica Microsystems, Deerfield, IL, USA) to further investigate microglia structure and morphology ...
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bioRxiv - Neuroscience 2019Quote: ... Frozen frontal cortex slides of 7-8 µm thickness were washed with the BondTM Wash Solution (Leica Biosystems, AR9590) for 15 min at room temperature and stained overnight with the primary antibodies at 4 °C (Kinesin heavy chain ...
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bioRxiv - Immunology 2021Quote: ... for F4/80 for 7 minutes at room temperature and EDTA at pH of 9.0 (Leica, Buffalo Grove, IL) for CD4 for 20 minutes on the immunostainer ...
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bioRxiv - Cell Biology 2022Quote: ... left lobes were embedded in paraffin wax and sectioned at 7-9μm thickness using a HM 325 rotary microtome(Leica). Sectioned slides were dried at room temperature until needed ...
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bioRxiv - Neuroscience 2023Quote: ... and MgCl2, 7 (pH 7.4, 320 mOsm) and 400μm coronal sections were cut using VT1200S vibratome (Leica Biosystems, Germany). The sections were allowed to recover for at least 30 minutes in recording aCSF (in mM) ...
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bioRxiv - Neuroscience 2022Quote: ... Frozen tissues were sliced coronally into 7 - 10 μm thick sections using a cryostat machine (Leica CM3050 S, Germany), mounted on glass slides and stored at −20 °C.
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bioRxiv - Cell Biology 2024Quote: ... Samples were embedded in paraffin and teratoma samples were serially sectioned into 7 µm sections using a microtome (Leica). Slices were stained with haematoxylin and eosin and cell types from the three embryonic layers were identified under the optic microscope (Nikon Eclipse Ni).
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bioRxiv - Developmental Biology 2024Quote: Ultrathin sections (60 nm) of Lowicryl HM-20-embedded testes were cut on a UC-7 ultramicrotome (Leica Biosystems) with a diamond knife (Diatome ...
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bioRxiv - Cell Biology 2022Quote: Ultrathin sections (60 nm) of Lowicryl HM-20-embedded follicles were cut on a UC-7 ultramicrotome (Leica Biosystems) with a diamond knife (Diatome ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... OCT-embedded lungs were cryosectioned in the coronal plane at 7 μm using a cryostat microtome (Leica CM 1510S). Cryosections were fixed on slides with 2% PFA for 15min at room temperature and washed three times with 1X PBS ...
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bioRxiv - Molecular Biology 2023Quote: A portion of skeletal muscle samples preserved in OCT were sectioned at 7 µm thickness using a cryotome (Leica Biosystems ...
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bioRxiv - Plant Biology 2020Quote: ... Sections (3-4μm) cut by rotary microtome (Leica RM2265) were stained with 0.01 % aqueous toluidine blue ...
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bioRxiv - Neuroscience 2021Quote: ... and 3% lead citrate (Leica Ultrostain II, Leica Microsystems). Images were collected semiautomatically using custom software guiding an FEI Magellan XHR 400L54.
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bioRxiv - Neuroscience 2021Quote: ... and 3% lead citrate (Leica Ultrostain II, Leica Microsystems). Images were collected semiautomatically using custom software guiding an FEI Magellan XHR 400L54.
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bioRxiv - Molecular Biology 2024Quote: ... 3 µm sections were cut on a microtome (Leica), rehydrated through an alcohol series according to standard laboratory procedures and then stained with a PAS staining kit (TCS Biosciences ...
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bioRxiv - Cancer Biology 2023Quote: ... 3 µm sections were obtained with a microtome (Leica RM2255 Fully Automated Rotary Microtome) ...
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bioRxiv - Bioengineering 2023Quote: ... and 20 min in 3% lead citrate (Leica, Germany). TEM images were acquired with a 200 kV TEM JEM 2100Plus (Jeol ...
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bioRxiv - Neuroscience 2021Quote: ... C57Bl/6j wildtype pups or >P30 mice were placed on a stereotaxic frame (Angle One; Leica, Germany). For the pups ...
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bioRxiv - Cell Biology 2019Quote: ... The chips were rotated onto one side on a glass slide coated with Glycerine-Solution (11513872, Leica) and the top side was covered with glycerin and a cover glass ...
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bioRxiv - Plant Biology 2021Quote: ... One-microliter drops of the suspensions were visualized in a Leica SPE Confocal Microscope (Leica Microsystems, Germany), using poly-L-lysine microscope slides.
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bioRxiv - Genomics 2020Quote: ... Microtome sections (5 μm, Leica RM2245) were mounted on adhesive slides (Klinipath-KP-PRINTER ADHESIVES) ...
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bioRxiv - Cell Biology 2024Quote: ... Microtome sections (5 µm, Leica RM2245) were mounted on adhesive slides (Klinipath-KP-PRINTER ADHESIVES) ...
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bioRxiv - Neuroscience 2021Quote: ... post-fixed in 4% PFA overnight at 4°C and afterwards cut with a vibratome (Leica Microsystems, Wetzlar Germany) in 100 μM thick sequential sagittal slices ...
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bioRxiv - Neuroscience 2023Quote: ... post-fixed in 4% PFA overnight at 4 °C and 100um thick coronal vibratome sections were made (Leica VT1000S). Images of both ipsilateral (injected ...
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bioRxiv - Neuroscience 2023Quote: ... Brains were postfixed overnight in 4% PFA at 4°C before 50μm coronal brain sections were cut using a vibratome (VT1200S, Leica). Slices were blocked (TBS with 0.5% Triton X-100 and 10% donkey serum ...
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bioRxiv - Neuroscience 2024Quote: ... Brains were extracted and post-fixed overnight at 4°C in 4% PFA (pH = 7.2) and 40-μm coronal sections were collected using a vibratome (VT1000S, Leica). After a 15-min incubation in a 4,6-Diamidino-2-phenylindole dihydrochloride hydrate (DAPI ...
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bioRxiv - Cancer Biology 2019Quote: ... was performed by the VCU Cancer Mouse Models Core Laboratory with the Leica Bond RX auto-stainer using heat-induced epitope retrieval buffer 1 (Leica, Sodium Citrate buffer, pH 6.0). Stained slides were then imaged on the Vectra Polaris (Akoya Biosciences) ...
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bioRxiv - Bioengineering 2021Quote: ... Samples were longitudinally cut into slices with a thickness of 1 mm and observed by confocal laser scanning microscopy (CLSM, Leica TCSSP8 MP, Leica microsystems, GER). Semi-quantitative analysis was conducted through the assay of the horizontal and vertical fluorescence intensity of the hybrid layer ...
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bioRxiv - Cancer Biology 2021Quote: ... Optimal primary antibody stripping conditions between rounds in the seven-color assay were performed following 1 cycle of tyramide deposition followed by heat-induced stripping (see below) and subsequent chromogenic development (Leica Bond Polymer Regine Detection DS9800) with visual inspection for chromogenic product with a light microscope by a senior pathologist (T.J.H.) ...
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bioRxiv - Molecular Biology 2023Quote: ... Zi-1 = 75.61μm) of pancreatic beta cells using the microscope’s TXR filter and a 40X objective (Leica HCX APO L U-V-I 0.80 W), at 20 msec exposures ...
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bioRxiv - Cancer Biology 2023Quote: ... Optimal primary antibody stripping conditions between rounds in the seven-color assay were performed following 1 cycle of tyramide deposition followed by heat-induced stripping (see below) and subsequent chromogenic development (Leica Bond Polymer Regine Detection DS9800) with visual inspection for chromogenic product with a light microscope (TH) ...
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bioRxiv - Biophysics 2019Quote: Live COS-7 cells expressing CCR5-mTFP1 were imaged using a SP8 X SMD confocal microscope Leica microscope from Leica Microsystems (Manheim ...
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bioRxiv - Physiology 2022Quote: Aortic roots at optimal cutting temperature were embedded in NEG-50 and cut into 7 μm thick sections using a cryostat (CM1510 S; Leica). These sections were stained with Oil Red O/hematoxylin to measure lipid depot using the protocol below ...
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bioRxiv - Neuroscience 2024Quote: ... The samples were then embedded in 7% agarose and cut into 0.3 mm sagittal sections using a vibratome (Leica VT1000s). The slices were incubated overnight at 37° C in PBS ...
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bioRxiv - Neuroscience 2023Quote: ... adult brains were fixed in 4% PFA and included in 7% low-melting agarose in PBS 1X and sectioned at 100 µm using a vibratome (Leica). Sections were mounted with Glycergel (Dako ...
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bioRxiv - Genomics 2023Quote: ... Sections were cut to a thickness of 7 μm with a Leica HistoCore AUTOCUT Rotary Microtome (Leica Biosystems, Wetzlar, Germany) and Leica RM2245 ...
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bioRxiv - Bioengineering 2023Quote: ... Images were taken with either a fluorescent light microscope (Axio Observer 7, Zeiss, Oberkochen, Germany) (Figure 3B and 5A and B) or a confocal microscope (TCS SP5X, Leica Microsystems CMS GmbH ...
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bioRxiv - Neuroscience 2020Quote: ... All mouse brain tissue specimens were fixed by immersion in 4% PFA overnight at 4 °C and sectioned at 50 μm using a vibratome (Leica).