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Citations for Leica :
1051 - 1100 of 1743 citations for 4' Trifluoromethoxy 5 trifluoromethyl 1 1' biphenyl 3 carboxylic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... Freeze substitution was performed over 3 d at −90°C in anhydrous acetone containing 1% OsO4 and 0.1% uranyl acetate using an automatic freeze substitution machine (EM AFS, Leica Microsystems, Vienna, Austria). Epon/Araldite infiltrated samples were then embedded in a thin layer of resin and polymerized for 3 d at 60°C ...
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bioRxiv - Neuroscience 2021Quote: Every 4th brain section was imaged as a Z-stack (1 μm step size) using either the Zeiss LSM 800 (Zen, v2.6.76) or Leica SP8 confocal microscope (Leica Application Suite X, v3.5.6.21594) at 20x magnification ...
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bioRxiv - Plant Biology 2020Quote: ... Light scattering changes in the presence of 50 μM MV were induced by applying red light (1,000 μmol m−2 s−1, Leica KL 1500 LED). Scattering changes were measured first in dark for 100 s ...
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bioRxiv - Cell Biology 2020Quote: ... Freeze substitution was performed over 3 days at –90 °C in anhydrous acetone containing 1 % OsO4 and 0.1 % uranyl acetate using an automatic freeze substitution machine (EM AFS, Leica Microsystems, Vienna, Austria) Serial semi-thick sections (300nm ...
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bioRxiv - Developmental Biology 2020Quote: ... Finally, embryos were cleared in BABB (benzyl alcohol: benzyl benzoate, 1:2) solution and vizualized under light microscope (Leica MZ6 Infinity1 stereomicroscope).
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bioRxiv - Cell Biology 2021Quote: ... Freeze substitution was performed over 3 d at -90 °C in anhydrous acetone containing 1 % OsO4 and 0.1 % uranyl acetate using an automatic freeze substitution machine (EM AFS, Leica Microsystems, Vienna, Austria). Epon/Araldite infiltrated samples were flat embedded in a thin layer of resin ...
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bioRxiv - Bioengineering 2022Quote: ... The samples were stored at −80°C for less than 1 week prior to sectioning (10 μm thick) using a CM1950 Cryostat (Leica, Wetzlar, Germany). Tissue sections were fixed and permeabilized in 1:1 methanol:acetone solution at −20°C ...
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bioRxiv - Cancer Biology 2024Quote: ... The slides were heated at 60°C for 1 h in an oven before H&E staining in the autostainer (Leica Autostainer XL). IHC was performed using a detection kit (Vector Laboratories ...
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bioRxiv - Molecular Biology 2023Quote: ... blotted with Whatman 1 filter paper and vitrified into liquid ethane at –180 °C using a Leica GP2 plunger (Leica microsystems, Austria). Frozen grids were transferred onto a Talos 200C Electron microscope (FEI ...
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bioRxiv - Molecular Biology 2023Quote: ... blotted with Whatman 1 filter paper and vitrified into liquid ethane at -180°C using a Leica GP2 plunger (Leica microsystems, Austria). Frozen grids were transferred onto a Talos 200C Electron microscope (FEI ...
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bioRxiv - Cell Biology 2023Quote: ... Imaging was performed using Leica DMI8 confocal microscope and HC PL APO CS2 20x objective (excitation, 488 nm, emission, 498 nm - 550 nm, pinhole size of 1 Airy Unit) (Leica Microsystems, Germany), pixel dimensions 0.48 μm px-1 ...
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bioRxiv - Plant Biology 2023Quote: 50 µm-thick cross sections were prepared from apical and basal internodes of Arabidopsis inflorescence stems at stage 1 (Altamura et al., 2001) by a vibrating blade microtome (Leica VT1200 S), stained with a 0.05% (w/v ...
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bioRxiv - Cancer Biology 2023Quote: ... Primary antibody incubation at 1:250 dilution for 30 minutes was followed by detection using the BOND™ Polymer Refine Detection System (DS9800, Leica Biosystems) as per manufacturer’s instructions.
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bioRxiv - Bioengineering 2023Quote: ... Slides were let dry for at least 1 h prior to imaging at the microscope (Leica DM 2000 LED fitted with a Leica DFC450c camera). Ten images from different regions of the slide were acquired and the number of invaded cells counted using custom-made software further described in the next section ...
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bioRxiv - Cancer Biology 2023Quote: ... Slides were immediately washed in running distilled water for 1 min prior to counterstaining cell nuclei with diluted Gill’s Hematoxylin (Leica Biosystems, Wetzlar, Germany). Slides were washed under running distilled water ...
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bioRxiv - Biochemistry 2023Quote: ... 3µL of the samples were dispensed onto glow discharged Quantifoil holey carbon grids (Cu R2/1 or R2/2) using a Leica GP plunger (Leica Microsystems, Austria) maintained at 90% humidity and 10°C ...
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bioRxiv - Biochemistry 2023Quote: 3 µl of the individual complexes were applied onto glow-discharged Quantifoil holey carbon grids (Cu R2/1 or R2/2) and vitrified in liquid ethane (−181 °C) using a Leica GP plunger (Leica Microsystems, Austria) maintained at 90% humidity and 10 °C ...
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bioRxiv - Neuroscience 2024Quote: ... 30μm serial sagittal sections were collected from Cx3cr1GFP/+ animals in a tempero-medial manner (1 in 12 series) using a vibratome (Leica VT 1200S) and kept as free-floating sections in ice-cold PBS ...
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bioRxiv - Microbiology 2024Quote: ... followed by fine trimming of an initial region of interest (ROI) spanning 2 x 2 x 1 mm using a glass knife and Leica UC7 ultramicrotome (Leica Microsystems, Austria). The trimmed block was then mounted on an ultramicrotome stub using conductive silver epoxy (SPI ...
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bioRxiv - Physiology 2024Quote: ... an area of each section (1 section/mouse) was acquired at 200x magnification using a confocal laser scanning microscope (Leica TCS SP8). Sequential scanning was employed using the 488 nm laser to visualize Alexa 488 and the 405 nm UV lasers to visualize DAPI staining ...
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bioRxiv - Neuroscience 2024Quote: 20X hippocampal tiles were acquired using a confocal microscope with a 16 μm z-dimension and 1 μm step size (Leica Stellaris 8). Images were opened in Imaris image analysis software (Oxford Instruments) ...
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bioRxiv - Molecular Biology 2024Quote: ... For scoring (graphs Figure 1) the percentage of cells expressing the chromatin markers were scored manually under an epi-fluorescence microscope (Leica DM6000 Leica microsystems, Germany).
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bioRxiv - Microbiology 2024Quote: ... we localized infection centers at this point of the protocol in reference to the gridded checkerboard by their HSV-1 mediated mChe expression using a wide-field fluorescence microscope (Leica Microsystems, Germany), and the filter set N2.1 containing the emission long pass filter LP590 which largely blocks the autofluorescence generated by the glutaraldehyde fixation ...
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bioRxiv - Pathology 2024Quote: Immunohistochemistry was performed using monoclonal mouse anti-CD8 (1:150, clone 4B11, NCL-CD8-4B11, Novocastra, Leica Microsystems, Newcastle Upon Tyne, UK) on sections from paraf-fin embedded tumour samples ...
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bioRxiv - Cancer Biology 2024Quote: Confirmation of proliferating cells and cell death were assessed by IF of Ki-67 (Leica, Cat# KI67-MM1-L-CE, 1:300) and DeadEnd Fluorometric TUNEL assay (Promega ...
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bioRxiv - Cell Biology 2024Quote: ... the myotubes were stained with hematoxylin and eosin (H&E) solution (BioOptica) for 1 min at room temperature and observed using an optical microscope (Leica, Wetzlar, Germany). For each condition ...
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bioRxiv - Bioengineering 2024Quote: ... Slides were allowed to dry for at least 1 h prior to imaging at the microscope (Leica DM 2000 LED fitted with a Leica DFC450c camera). Ten images from different regions of the slide were acquired [35] ...
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bioRxiv - Plant Biology 2024Quote: ... a 1-μl sample was diluted in a 1:10 ratio with 10% (w/v) sucrose and observed under a stereomicroscope (Leica, M165 FC). Then ...
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bioRxiv - Cancer Biology 2021Quote: ... pre-cleared lysates were incubated with 2-4 µl p53 polyclonal antibody (Leica Biosystems, NCL-L-p53-CM5p) overnight at 4°C on a nutator to allow p53 protein complexes to form ...
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bioRxiv - Genetics 2021Quote: ... Brains were post-fixed in 4% polymeric formaldehyde and sliced into 30 μm sections using a vibratome (Leica) for histological analyses ...
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bioRxiv - Developmental Biology 2021Quote: ... Formalin-fixed samples were fixed in paraffin and 4 μm sections were cut using an RM2255 microtome (Leica) and immobilized to adhesion slides (SuperFrost Plus ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were finally mounted in 4’,6-diamidino-2-phenylindole (DAPI) and examined using fluorescence microscope (Leica DMi8). The first antibodies include rabbit anti-Iba1 (Wako) ...
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bioRxiv - Developmental Biology 2022Quote: ... adult mouse brain tissue samples were embedded in 4% agarose and sections were cut using vibratome (Leica VT1200S). Until further processing sections were stored in ice-cold 1xPBS ...
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bioRxiv - Developmental Biology 2022Quote: ... The embryos embedded in paraffin blocks were serially sectioned at 4 μm thickness with a microtome (Leica RM2035) and placed on microscopic glass slides (Superfrost Plus ...
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bioRxiv - Immunology 2021Quote: ... Each organ was sliced into 4 µm sheets using a RM2235 manual microtome (Leica Biosystems, Buffalo Grove, IL) and imaged with a DMi1 optical microscope (Leica Biosystems ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were finally mounted in 4’,6-diamidino-2-phenylindole (DAPI) and examined using fluorescence microscope (Leica DMi8). Embryonic and postnatal ventral midbrain tissues were fixed with 4% paraformaldehyde and dehydrated with 30% sucrose overnight ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were finally mounted in 4’,6-diamidino-2-phenylindole (DAPI) and examined using fluorescence microscope (Leica DMi8). The first antibodies include rabbit mouse anti-sarcomeric α-actinin (SAA ...
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bioRxiv - Neuroscience 2021Quote: ... Brainstems were embedded in 4% agarose (in PBS) and sliced coronally using a Vibratome (Leica VT1000s, Nussloch, Germany) at either 200 µm thickness for myelination analysis with CARS or 70 µm thickness for labeling oligodendrocytes.
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Formalin-fixed paraffin-embedded tissue sections (4 μm) were first deparaffinized on an automated platform (Leica Autostainer XL). After deparaffinization ...
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bioRxiv - Cell Biology 2020Quote: ... a 4 mm diameter circle was etched on the bottom of the coverslip with a diamond objective (Leica) to indicate the area of interest during sample processing for EM ...
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bioRxiv - Pathology 2022Quote: ... Cells were finally mounted in 4’,6-diamidino-2-phenylindole (DAPI) and examined using fluorescence microscope (Leica DMi8). The first antibodies are listed in Supplementary Table S2.
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bioRxiv - Neuroscience 2022Quote: ... at 4°C before being sliced into 40 μm slices on a freezing microtome (Leica, Wetzlar, Germany; SM2000R). Brain sections were pre-incubated with a blocking buffer (0.5% Triton X-100 (Sigma ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were finally counterstained with 4’,6-diamidino-2-phenylindole (DAPI) and examined using fluorescence microscope (Leica DMi8).
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bioRxiv - Cancer Biology 2024Quote: ... Microscopic samples were kept at 4 °C until further analyses using the Leica SP8x (Leica Microsystems Co., USA) laser scanning confocal microscope ...
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bioRxiv - Cancer Biology 2024Quote: ... These blocks were then sectioned at a thickness of 4 µm and stained using Harris haematoxylin (Leica Surgipath) and alcoholic eosin-Y stain (Leica Surgipath) ...
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bioRxiv - Cell Biology 2023Quote: ... as described previously.4 Tissues were imaged using an SP5X laser scanning confocal microscope (Leica Microsystems CMS GmbH). Fluorescent signal distribution along gastric glands was quantified with Fiji software.
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bioRxiv - Neuroscience 2023Quote: ... frozen tissue blocks from each of the 4 AD subjects were sectioned at 10 micron thickness (Leica, CM3050s) and stored at −80°C ...
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bioRxiv - Neuroscience 2023Quote: ... it was embedded in 4% agar and sectioned into 100-µm slices using a vibraslicer (VT1200S, Leica Biosystems). The slices were mounted on glass slides with anti-fade media (#S36963 ...
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bioRxiv - Plant Biology 2023Quote: ... embedded in 4% low melting agarose and sectioned into 150 µm cross sections using the vibratome VT1000s (Leica). Images were acquired immediately after sectioning on the LSM800 using a 63.0 x oil immersion objective (Zeiss) ...
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bioRxiv - Cell Biology 2023Quote: ... They were kept at 4°C prior to image acquisition using Cell DIVE Multiplex Slide Scanner (Leica Microsystems). For all transplant experiments ...