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Citations for Leica :
1001 - 1050 of 1513 citations for 4 1 1 2 3 3 3 Hexafluoropropoxy acetophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... embedded in 4% low melting agarose and sectioned into 150 µm cross sections using the vibratome VT1000s (Leica). Images were acquired immediately after sectioning on the LSM800 using a 63.0 x oil immersion objective (Zeiss) ...
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bioRxiv - Neuroscience 2024Quote: ... Brains were cut into 4 series of 30 µm coronal sections using a freezing microtome (Leica SM 2010R) and stored at −20°C in cryoprotectant until used ...
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bioRxiv - Plant Biology 2024Quote: ... the samples were coated with platinum (4 to 10 nm) in a EM MED020 vacuum coating system (Leica) and observed in a scanning electron microscope (ESEM Quanta 250 FEG FEI ...
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bioRxiv - Physiology 2024Quote: ... apparatus The tissues were embedded in paraffin (Roth) sliced in 4-5 μm sections using a microtome (Leica). The sections underwent deparaffination and rehydration procedures ...
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bioRxiv - Bioengineering 2024Quote: ... the samples were paraffin-embedded and cut into 4–5 μm sections using a microtome (RM2125, Leica; Germany). These sections were then stained with hematoxylin and eosin (H&E ...
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bioRxiv - Neuroscience 2021Quote: ... then sectioned coronally into 20-μm-thick slices (1:6 series of the PVN) using a Leica CM1900 cryostat (Leica Microsystems, Wetzlar, Germany). Immunofluorescence staining was performed on brain sections derived from P10 male tdTomato-Crh (Crh-IRES-Cre;Ai14 ...
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bioRxiv - Bioengineering 2022Quote: ... and stained using immunohistochemistry (IHC) for collagen I (1:4000, polyclonal rabbit, lifespan biosciences, Seattle WA) via a Bond-Max autostaining system (Leica biosystems, Lincolnshire, IL). Stained kidneys were scanned at high resolution using a CRi Panoramic SCAN 40x Whole Slide Scanner (Perkin-Elmer).
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bioRxiv - Immunology 2022Quote: ... and stained using immunohistochemistry for collagen I (1:4000, polyclonal rabbit, lifespan biosciences, Seattle WA) via a Bond-Max autostaining system (Leica biosystems, Lincolnshire, IL). Stained kidneys were scanned at high resolution using a CRi Panoramic SCAN 40x Whole Slide Scanner (Perkin-Elmer).
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bioRxiv - Plant Biology 2020Quote: ... Thick sections (1 µm) of long shoots were cut with glass knives using an Ultracut R microtome (Leica Microsystems, Buffalo Grove, IL, USA) and stained with toluidine blue ...
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bioRxiv - Immunology 2021Quote: ... z-stacks were generated with a 20x objective at a z-step of 1 µm using a SP8 laser scanning confocal microscope (Leica Biosystems, Nußloch, Germany). Composite images were generated using ImageJ with Fiji distribution [31].
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bioRxiv - Neuroscience 2021Quote: ... isogenic samples reared in the same environment were highpressure frozen (Leica HPM100 for datasets 1-5 and Leica ICE for datasets 6-8) at different stages of post-embryonic development ...
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bioRxiv - Immunology 2022Quote: ... and stained using immunohistochemistry (IHC) for collagen I (1:4000, polyclonal rabbit, lifespan biosciences, Seattle WA) via a Bond-Max autostaining system (Leica biosystems, Lincolnshire, IL). Stained kidneys were scanned at high resolution using a CRi Panoramic SCAN 40x Whole Slide Scanner (Perkin-Elmer).
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bioRxiv - Pathology 2021Quote: ... Slides were then stained with anti-Iba1 antibody (1:2000; Wako; 019- 19741 Richmond, VA) using the Bond Polymer Refine Red Kit (cat. DS9390 Leica Biosystem, Richmond, IL). The slides were stained using a pan-cytokeratin antibody (1:1000 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... RNAscope staining was followed by the C219 IHC at a 1:200 dilution for 30 minutes on a Bond RX auto-stainer using the Bond Polymer Refine Kit (Leica, Buffalo Grove, IL) minus DAB & Hematoxylin ...
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bioRxiv - Neuroscience 2021Quote: ... Regularly spaced brain series (5 × 1-in-5) of 30 μm-thick frozen sections were cut in freezing microtome (SM2000R, Leica, Wetzlar, HE, Germany) in the coronal plane ...
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bioRxiv - Neuroscience 2021Quote: images were obtained using the Axio Imager 1 microscope (Zeiss, Oberkochen, Germany) and confocal images were obtained with a laser scanning confocal microscope (Leica SPE, Mannheim, Germany) associated with a Leica LAS AF interface ...
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bioRxiv - Cell Biology 2020Quote: ... samples for the projects as listed in Table 1 were cryo-immobilized by using the following high-pressure freezers: EMPACT2+RTS (Leica Microsystems, Vienna, Austria), Leica EM ICE (Leica Microsystems ...
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bioRxiv - Microbiology 2023Quote: ... Z stacks of horizontal plane images were acquired in 1 μm steps using a Leica SP8 AOBS inverted laser scanning microscope (CLSM, LEICA Microsystems, Wetzlar, Germany) at the INRAE MIMA2 platform (33) ...
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bioRxiv - Neuroscience 2022Quote: ... Brains were then frozen and coronally sectioned at a thickness of 30 μm (1:5 series) using a Leica CM1900 cryostat (Leica Microsystems, Wetzlar, Germany). Fos2A-iCreER+/-::Ai14+/- sections were mounted on gelatin-coated slides and coverslipped with Vectashield containing DAPI (cat ...
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bioRxiv - Physiology 2023Quote: ... ten H&E stained images of 1 mm2 (4,000,000 pixel2) were randomly extracted from the entire adipose tissue image using Aperio ImageScope (Leica Biosystems Imaging, Deer Park, IL) and analyzed with the MorphoLibJ collection of mathematical morphology methods and plugins using the image processing package FIJI [36] ...
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bioRxiv - Cell Biology 2023Quote: ... On day 1 after seeding the grids were incubated with 10 nm colloidal gold particles for 1 minute and rapidly frozen in liquid ethane using GP2 plunge-freezer (Leica Microsystems, Vienna, Austria). Tilt series of vitrified samples were collected using a Tecnai F20 microscope (FEI Company ...
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bioRxiv - Neuroscience 2023Quote: ... P14-P16 and P20-P21 pups of both sexes were placed in a perfused chamber at room temperature (22-25°C) in oxygenated Mg2+ containing ACSF (ACSF-1) medium under a Leica DMLFS microscope (Leica Microsystems Inc., USA). The recordings were performed using a coated nichrome electrode with an Iso-DAM8A World Precision Instruments amplifier (World Precision Instruments ...
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bioRxiv - Microbiology 2023Quote: ... Z-stacks of horizontal plane images were acquired in 1 μm steps using a Leica SP8 AOBS inverted laser scanning microscope (CLSM, LEICA Microsystems, Wetzlar, Germany) at the INRAE MIMA2 platform (doi.org/10.15454/1.5572348210007727E12)54.54 ...
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bioRxiv - Plant Biology 2024Quote: ... immersed in 1-Hexadecene as cryoprotectant and immediately fixed by high pressure freezing (HPF) using the Leica EM ICE (Leica Microsystems GmbH, Germany). Freeze substitution and embedding of the HPF-fixed samples were conducted in a temperature-controlled device ...
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bioRxiv - Neuroscience 2024Quote: ... together with Opal 520/570/690 Fluorophore Reagent pack detection (1:1000, Cat #FP1487001KT/FP1488001KT/FP1497001KT) on the Leica BOND RX Fully Automated Research Stainer (Leica, Buffalo Grove, IL) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... 7A) were performed under an OPMI 1 FR PRO Surgical microscope (Zeiss, Jena, Germany) or Leica S9D Stereo microscope (Leica Microsystems, Wetzlar, Germany). A 30G disposable needle was used to puncture the sclera near the limbus ...
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bioRxiv - Cancer Biology 2024Quote: ... in TBST buffer for IF and subjected to staining with the following antibodies overnight at 4C: p53 (Leica, P53-CM5P-L 1:100), GFP/YFP (Abcam ...
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bioRxiv - Cancer Biology 2024Quote: ... Primary antibody (dilution at 1: 100) was incubated for 15 min at room temperature and detected using the Bond Polymer Refine Detection kit (Leica Biosystems, Cat# DS9800) with DAB as chromogen ...
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bioRxiv - Cell Biology 2020Quote: ... 4 µm thick sections of PC and DC were produced using a Leica CM3050 S Research Cryostat (Leica Microsystems) and mounted onto microscope slides ...
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bioRxiv - Developmental Biology 2022Quote: ... The sample was sectioned to a thickness of 4 µm using a Leica Autostainer XL (Leica Biosystems, Nussloch, Germany) and stained with hematoxylin and eosin (H&E) ...
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bioRxiv - Neuroscience 2020Quote: ... Mounted sections were stored at 4°C until they were imaged using a laser scanning confocal microscope (Leica SP8).
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bioRxiv - Neuroscience 2020Quote: ... and post-fixed in 4% PFA overnight at 4ºC before 120μm sections were cut using a vibratome (Leica, VT100S). For whole mount staining ...
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bioRxiv - Neuroscience 2021Quote: ... in 0.1M phosphate buffered saline (PBS, Fisher #BP399-20) at +4°C before slicing on a freezing microtome (Leica) in a 1:12 series of 40 µm thick coronal sections ...
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bioRxiv - Biophysics 2020Quote: ... Images were acquired every 4 s with an ORCA-Flash4.0 C13440-20C camera (Hamamatsu photonics, Hamamatsu, Japan) controlled by LAS X software (Leica). Simultaneously ...
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bioRxiv - Neuroscience 2022Quote: ... cerebral organoids were fixed in 4% PFA and sectioned into 10 µm thick slices with a cryostat (CM1950, Leica). Images were captured via a Leica TCS SP8 STED confocal microscope equipped with a LAS X software ...
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bioRxiv - Immunology 2022Quote: ... for 4 min at room temperature and observed them under a Laserscaing Confocal Microscopy (TCS SP8 STED, Leica, German). The fat body and midgut stained cells were analyzed with automated image analysis software by a confocal laser scanning microscopy (FluoView FV1000 ...
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bioRxiv - Developmental Biology 2020Quote: ... embedded in Moviol and stored at 4 °C until analysis using a confocal laser scanning microscope (TCS SP1, Leica)
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bioRxiv - Plant Biology 2023Quote: ... The samples were washed with 0.05 M sodium cacodylate buffer and embedded in 4% agarose and sliced using VT1000S vibratome (Leica). For localization and auxin/cytokinin distribution pattern ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... and were kept at 4°C until being coronally sectioned (100 μm sections) with a Vibratome (VT1000S, Leica, Germany). Sections were mounted in Vectashield mounting medium containing DAPI (H1500 ...
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bioRxiv - Neuroscience 2023Quote: ... Whole brains were carefully dissected and post-fixed into 4% PFA overnight and sliced using a vibratome (Leica VT1200). Coronal serial sections were made at a thickness of 70μm in a 4 °C PBS solution ...
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bioRxiv - Plant Biology 2022Quote: ... Stained seed coats were fixed with 4-5% agarose gel and dissected by microtome (Leica, VT1000 S, Wetzlar, Germany) and were visualized by Nikon 80i-epi-microscope ...
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bioRxiv - Microbiology 2023Quote: ... and Plan Apochromat 63×/1.4 objective lens or a Leica SP8 Laser Scanning Confocal Microscope equipped with HC PL APO 20x/0.75 (Leica), HC PL APO 40x/1.3 or HC PL APO 63x/1.40 and Leica Application Suit X software (LAS X ...
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bioRxiv - Physiology 2024Quote: Pancreas were dissected and directly fixed in 4% paraformaldehyde overnight before embedding in paraplast (Paraplast X-tra, 39603002, Leica). 6 μm-thick sections were produced ...
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bioRxiv - Cell Biology 2024Quote: ... Paraffinized tissue blocks were sliced at a 4-μm thickness using a Sliding Microtome SM2000R (Leica Biosystems, Wetzlar, Germany) and processed for IHC staining ...
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bioRxiv - Bioengineering 2024Quote: ... embedded in 4 % agarose and cut at a thickness of 150 µm using a vibratome (VT1000S vibratome, Leica Biosystems). Brain slices were stained with Hoechst 33342 (10 µg/ml ...
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bioRxiv - Neuroscience 2024Quote: ... flattened hemispheres were embedded in agar gel 4% before cutting slices of 60µm thickness with a vibratome (VT 1000 S, Leica).
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bioRxiv - Neuroscience 2024Quote: ... the brains were embedded in 4% agarose/10% sucrose and sectioned at 50 µm using a vibratome (Leica VT1200S). Sections were stored at −20°C in a glycol mixture until use ...
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bioRxiv - Neuroscience 2024Quote: ... The samples embedded in paraffin were sagittally sectioned at 4 μm using a microtome HistoCore Biocut (Leica, Madrid, Spain). Sections corresponding to similar brain coordinates were deparaffined and rehydrated ...
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bioRxiv - Plant Biology 2024Quote: ... The resulting resin blocks were then sectioned into 4 μm slices using a Leica Histocore AUTOCUT rotary microtome (Leica) and stained with either 0.01% (w/v ...
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bioRxiv - Neuroscience 2021Quote: ... contusion was induced using a 2 mm convex tip attached to an electromagnetic impactor (Leica). The contusion depth was set to 1.25 mm from dura with a velocity of 4.0 m/s sustained for 300 ms ...