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Citations for Leica :
951 - 1000 of 1523 citations for IL 1 beta Rat since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... 8–10 week old mice received a single impact onto the skull at bregma using an ImpactOne stereotaxic impactor (Leica Biosystems, Buffalo Grove, IL) with a 3-mm-diameter tip (velocity set at 4.0 m/s ...
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bioRxiv - Cancer Biology 2020Quote: Five micron sections of tumors were immunolabeled with one of the following antibodies using a Leica Bond III automated immunohistochemistry instrument (Leica Biosystems, Buffalo Grove, IL): Rabbit anti-CD3 ...
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bioRxiv - Cancer Biology 2021Quote: ... IHC staining for CAV1 was performed on formalin-fixed paraffin-embedded HER2-expressing gastric tumor sections (5 μm thickness) on a Leica Bond RX automated stainer (Leica Biosystems, Buffalo Grove, IL). Following deparaffinization and heat-induced epitope retrieval in a citrate buffer at pH 6.0 ...
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bioRxiv - Neuroscience 2021Quote: ... and sacrificed via transcardiac perfusion with 10% sucrose (isotonic at 9.25% in distilled water; prewash) and 4% paraformaldehyde (PFA; fixative) using the Perfusion Two™ Automated Pressure Perfusion System (Leica Biosystems, Buffalo Grove, IL) (n = 12 ...
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bioRxiv - Neuroscience 2022Quote: ... and images of the sections were taken at 10x and 20x magnification using Leica DMI3000 B manual inverted fluorescence microscope (Leica Microsystems, Buffalo Grove, IL).
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bioRxiv - Cancer Biology 2022Quote: ... paraffin-embedded whole-tissue sections from decalcified human bone marrow core biopsy specimens in a Bond RX automated tissue stainer (Leica Biosystems, Buffalo Grove, IL), as described previously91 ...
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bioRxiv - Neuroscience 2022Quote: ... The cryoprotected blocks were then flash-frozen using dry ice and sectioned at 40 μm using a Leica SM2000R sliding microtome (Leica Biosystems, Deer Park, IL). For immunohistochemistry ...
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bioRxiv - Neuroscience 2023Quote: ... the brains were quickly removed from the skull and cut into 200 µm thick coronal slices using a Leica vibratome VT 1200S (Leica Biosystems, Buffalo, IL, USA). The slices were then incubated for 30 minutes at 34 °C in the isolation NMDG buffer and for another 30 minutes in artificial cerebrospinal fluid (aCSF ...
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bioRxiv - Cancer Biology 2024Quote: ... Slides were scanned using the Aperio AT2 digital scanning system and HAF nuclear staining intensity quantitation was performed using Aperio digital imaging software (Leica Biosystems, Buffalo Grove IL) and statistical analysis performed using unpaired Student’s T-tests in GraphPad Prism software 10.10.2 (GraphPad Software ...
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bioRxiv - Neuroscience 2022Quote: ... embedded them in 4% w/v agar/PBS and prepared 40 µm thick slices using a vibrating blade microtome (VT1200S, Leica Microsystems, Buffalo Grove, IL). The slices were post-fixed in 4% PFA/PBS for 30 min at room temperature (RT ...
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bioRxiv - Neuroscience 2022Quote: ... 0.4 mM L-ascorbic acid and the pH was adjusted to 7.4) and then sliced on a vibratome (Leica, VT1200 S, Deerfield, IL, USA). Brain slices (300 μm thick ...
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bioRxiv - Neuroscience 2022Quote: ... The brains were embedded in 3% agarose gel in a cubic cryomold and coronally sectioned at 50 μm thickness using a VT1000S vibrating blade microtome (Leica Biosystems, Buffalo Grove, IL). The sections were rinsed in PBS ON at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... The brass planchets were carefully removed from the polymerized resin and semi-thin (300 nm) sections were cut on a Leica Reichart Ultracut (Leica Microsystems, Buffalo Grove, IL), stained with 0.5% Toluidine Blue in 1% aqueous sodium borate (Fisher ...
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bioRxiv - Cell Biology 2022Quote: ... The ratio of bacterial particles per macrophage was quantified by taking photographs of three fields for each well using a Leica DM4000 B microscope (Leica Microsystems, Buffalo Grove, IL) with a 20x objective.
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bioRxiv - Cell Biology 2024Quote: ... Cat# 493558-C2) with the RNAscope LS Multiplex Fluorescent Assay (ACD, Cat# 322800) using the Bond RX auto-stainer (Leica Biosystems, Deer Park, IL) with a tissue pretreatment of 15 minutes at 95°C with Bond Epitope Retrieval Solution 2 (Leica Biosystems) ...
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bioRxiv - Neuroscience 2024Quote: ... Sections for screening tissue and serial sections were obtained on an automated tape ultramicrotome (ATUM; Hayworth et al., 2006; Schalek et al., 2012) and Leica EM UC6 ultramicrotome (Leica Microsystems, Buffalo Grove, IL) at 30-35 nm with 4 mm 35° diamond knives (DiATOME ...
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bioRxiv - Neuroscience 2024Quote: ... 15.l/min) male mice were impacted over the left somatosensory cortex using an electromagnetic impactor (Leica Impact One Stereotaxic impactor, Leica Biosystems, Richmond, IL, USA) with a 3 mm round tip (3 m/s ...
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bioRxiv - Neuroscience 2023Quote: ... Brains were immediately post-fixed in 10% formalin and sectioned at 40 microns through the DLS on a cryostat (CM1850, Leica Biosystems, Buffalo Grove, IL). Sections were then stained with cresyl violet to improve visualization ...
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bioRxiv - Neuroscience 2022Quote: ... mice were anaesthetized using 2.5% isoflurane and 1.5 l/min air for 5 min and impacted directly over their intact head using an electromagnetic impactor (Leica Impact One Stereotaxic impactor, Leica Biosystems, Richmond, IL, USA) with a 3mm round tip at a speed of 3m/s ...
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bioRxiv - Cancer Biology 2023Quote: ... NAT was chosen from each of 11 tumors with available slides and subjected to whole slide scanning at 400× magnification using an Aperio XT scanner (Leica Biopsystems, Buffalo Grove, IL). Images were visualized using ImageScope software (Leica Biosystems) ...
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Hypothalamic melanocortin-4 receptors on astrocytes mediate inflammation and body weight homeostasisbioRxiv - Neuroscience 2022Quote: Slides for validation and inflammation studies were imaged using a Leica M205-MFC 2D/3D THUNDER large specimen imaging system microscope (Leica Microsystems, Buffalo Grove, IL) with the appropriate filters for the applied fluorophores ...
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bioRxiv - Physiology 2022Quote: ... and imaged immediately using a confocal laser scanning microscope equipped with a white light laser (TCS Leica SP8 X; Leica Microsystems Inc., Deerfield, IL). The MitoTracker dye was excited at 490 nm and TMRE excited at 552 nm ...
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bioRxiv - Bioengineering 2022Quote: ... Samples were washed an additional 3 times in 100% ethanol for 15 minutes each and dried using a Leica EM CPD030 critical point dryer (Leica Microsystems, Buffalo Grove, IL) with CO2 as the transitional medium ...
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bioRxiv - Neuroscience 2023Quote: ... 10x and 20x images of left and right regions of interest (ROIs) for GFAP slides were photographed using an Axio lmager Z1 microscope with an Apotome (Leica Microsystems, Buffalo Grove, IL). For measurement of GFAP MFI ...
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bioRxiv - Neuroscience 2023Quote: ... Brains were then blocked for the PVN and 250 µm coronal slices were prepared on a Leica VT 1000S vibratome (Leica Biosystems, Buffalo Grove, IL) and transferred to a holding chamber with 34°C heated and carbogen saturated aCSF (in mM ...
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bioRxiv - Plant Biology 2023Quote: ... 5 μl was transferred into an HPF flat specimen carrier and frozen with a Leica EM PACT high-pressure freezer (Leica Microsystems, Inc., Bannockburn, IL) at a typical rate of 1,700°C/s ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were scanned to create digital images with either NanoZoomer (Hamamatsu Photonics, Japan) or Aperio (Aperio AT2 - High Volume, Digital whole slide scanning scanner, Leica Biosystems, Deer Park, IL) digital slide scanning systems and images were captured and exported with NDP viewer (Hamamatsu Corporation ...
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bioRxiv - Neuroscience 2023Quote: ... using Leica Bond III automated staining system with diaminobenzidine chromogen detection and the same antibodies as for clinical diagnostics (DS9800, Leica Biosystems, Buffalo Grove, IL). Hematoxylin and eosin stain was used for general histopathology ...
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bioRxiv - Cell Biology 2022Quote: ... One 5um section from each tissue type was stained with hematoxylin and Eosin (H&E) and scanned at 20X magnification using an Aperio AT2 (Leica Biosystems, Buffalo Grove, IL) into whole slide digital images.
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bioRxiv - Cell Biology 2022Quote: ... or for 4–6 s at 4°C and ~ 86% and plunge frozen using a Leica EM GP (Leica Microsystems Inc, Buffalo Grove, IL). The samples were imaged in an FEI Titan Krios electron microscope operated at 300 kV with a K2 Summit camera and energy filter (Gatan ...
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bioRxiv - Cell Biology 2023Quote: ... with a Z-step size of 0.3 μm using the Leica Application Suite X (LAS X) acquisition software (Leica Microsystems Inc., Buffalo Grove, IL). All imaging was done with LED8 light sources ...
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bioRxiv - Molecular Biology 2023Quote: ... The membranes were then cut out and mounted on a glass slide for imaging with a DMi8 inverted fluorescent microscope (Leica Microsystems, Buffalo Grove, IL).
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 100 tiles were imaged at 10X magnification with a color camera (DMC-4000) using a DMi8 automated microscope (Leica Microsystems Inc., Buffalo Grove, IL). Pixels were quantified using ImageJ ...
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bioRxiv - Systems Biology 2023Quote: ... paraffin-embedded whole-tissue sections from decalcified human BM core biopsy specimens in a Bond RX automated tissue stainer (Leica Biosystems, Buffalo Grove, IL), as described previously 80,81,87,88 ...
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bioRxiv - Biochemistry 2023Quote: ... the grids were flash-frozen in liquid ethane at ∼90 % humidity and 4 °C with a Leica EM GP rapid-plunging device (Leica, Buffalo Grove, IL, USA) before being transferred into liquid nitrogen for storage ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The entire transverse section was also analyzed for fibrosis expressed as a percentage of total area using an Aperio ImageScope macro (Leica Biosystems, Deer Park, IL).
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bioRxiv - Neuroscience 2023Quote: ... Cryosections from human and mouse spinal cord or DRGs were cut at 12 μm using a freezing microtome (CM1850; Leica, Buffalo Grove, IL, USA) and incubated at 4°C overnight with primary antibodies including ...
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bioRxiv - Cell Biology 2023Quote: ... Cryosections (8 μm) were cut on a cryostat HM 505E (Microm, Walldorf, Germany) equipped with a CryoJane Tape-Transfer system (Leica Inc., Buffalo Grove, IL). For labeling ...
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bioRxiv - Neuroscience 2023Quote: ... 40µm (or 80µm)-thick sections containing M1 were prepared through the anterior to posterior axis using a Leica VT1200S Vibratome (Leica Microsystems, Buffalo Grove, IL, USA). After slice preparation ...
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bioRxiv - Bioengineering 2023Quote: ... were acquired over a ∼30 mm x ∼3 mm rectangular area by using the tile scan feature of the LAS X software (Leica Microsystems, Buffalo Grove, IL). This involved frame by frame acquisition (36 × 5 = 180 frames ...
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bioRxiv - Neuroscience 2023Quote: ... The extracted brain was blocked coronally with a brain matrix (Zivic Instruments; Pittsburg, PA) and acute coronal slices (300 µm) were cut on a vibratome (VT1000S, Leica Microsystems; Buffalo Grove, IL) through the striatum in ice-cold ...
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bioRxiv - Microbiology 2024Quote: ... for detection of Influenza virus A H5N1 nucleoprotein (NP) antigen was performed on the automated BOND RXm platform and the Polymer Refine Red Detection kit (Leica Biosystems, Buffalo Grove, IL). Following automated deparaffinization ...
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bioRxiv - Neuroscience 2024Quote: ... All histological images were acquired on a Nikon fluorescence microscope (Eclipse Ni-U, Melville, NY, USA) and confocal imaging system (Leica Microsystems, Deerfield, IL, USA). Areas of interest were the somatosensory cortex and thalamus regions of the brain ...
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bioRxiv - Microbiology 2024Quote: ... and the RNAscope® ISH assay was performed using the RNAscope 2.5 LSx Reagent Kit (ACD) on the automated BOND RXm platform (Leica Biosystems, Buffalo Grove, IL, USA). Following automated baking and deparaffinization ...
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bioRxiv - Developmental Biology 2021Quote: ... Electroporation efficiency and extent of overexpression were evaluated at the time of embryo collection by detecting GFP and RFP on a stereo dissecting microscope under epifluorescent illumination (MZFLIII-TS, Leica Microsystems, Buffalo Grove, IL, USA).
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bioRxiv - Cell Biology 2019Quote: ... GFPdgn direct fluorescence (green) and the stained F-actin (red) were visualized and imaged using a Leica TCS SP8 confocal microscope (Leica Microsystems Inc., Buffalo Grove, IL).
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bioRxiv - Cancer Biology 2019Quote: ... These cross-sections of the lungs were stained with H&E and scanned by an Aperio ScanScope® CS System (Leica Biosystems, Buffalo Grove, IL). The metastatic burden was calculated as the percentage of metastasis/lung area and the number of foci/lung area ...
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bioRxiv - Physiology 2021Quote: ... All sections of both patellae and femora were stained with Safranin-O/Fast Green and imaged at 20x magnification using a slide imaging system (Aperio, Leica 122 Biosystems, Buffalo Grove, IL). Two blinded raters scored each section using the Osteoarthritis Research Society International (OARSI ...
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bioRxiv - Cell Biology 2021Quote: ... INS-1E cells were incubated with HO-PI for 15 min (5 mg/ml) and observed using a confocal laser-scanning microscope (Leica SP5; Leica Microsystems, Buffalo Grove, IL, USA).
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bioRxiv - Bioengineering 2020Quote: ... and polymerized at 60 °C for 48 h.[73] Ultrathin sections of ∼70 nm were produced in an ultra-microtome (Leica Biosystems, Buffalo Grove, IL, USA), transferred to copper grids (Sigma-Aldrich ...