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Citations for Leica :
951 - 1000 of 1673 citations for 2 1 ethylpentyl 1 3 dioxan 5 yl laurate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Paraffin-embedded sections were cut in 5 µm thick slices with a microtome (SM 2010R Leica) and affixed to Superfrost microscope slides (VWR #631-0108) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 5 μm sections were then taken on slides by cutting the paraffin blocks using microtome (Leica) and stained for Hematoxylin & Eosin and Trichrome ((HT10516-500ML ...
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bioRxiv - Plant Biology 2023Quote: Nodules were embedded in 4-5% agarose and sliced in 100um sections using a vibratome (Leica). Samples were immediately imaged on slides using a 20x air objective using the excitation and emission settings described above ...
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bioRxiv - Bioengineering 2024Quote: ... The samples were cut into 5 μm thick sections by using a microtome (RM2235, Leica, DEU) and stored at −20 °C until further use ...
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bioRxiv - Neuroscience 2024Quote: The live imaging chamber was transferred to the confocal microscope stage (Leica Stellaris 5 or SP8), and the samples were imaged under controlled environmental conditions (37°C ...
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bioRxiv - Immunology 2024Quote: ... Tumors were sectioned to a thickness of 5 µm using the rotary microtome cryostat machine (Leica). A circle drawn with a hydrophobic pen around the tissue section was used to provide surface tension ...
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bioRxiv - Immunology 2024Quote: ... 5 to 10-minute-long videos were recorded using an inverted Leica SP8 microscope (Leica Microsystems). Image analysis was performed with Fiji.
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bioRxiv - Neuroscience 2023Quote: ... All tissues were obtained at 5 um thickness using a Leica CM 1859 cryostat (Leica, Germany) and stored at -20°C until use ...
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bioRxiv - Bioengineering 2023Quote: ... The frozen tissue sample was then sectioned to 5 μm with a cryostat machine (cm1950, Leica).
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bioRxiv - Immunology 2023Quote: ... Time-lapse images were acquired with a Leica SP8 inverted 5 channels confocal microscope (Leica Microsystems) equipped with 40× oil objective ...
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bioRxiv - Physiology 2022Quote: ... Cryosections (5 μm thick) were cut using a Leica 3050S Microtome (Leica Microsystems, Buffalo Grove, IL). Sections were picked up on positively charged slides (Thomas scientific ...
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bioRxiv - Cell Biology 2024Quote: ... fluorescence images were captured in 5 different regions using a DMI 3000 B microscope (Leica, Germany), and analysed using the NIH imaging software Image J 64 ...
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bioRxiv - Neuroscience 2024Quote: ... Every third section of the dorsal brainstem was imaged on a confocal microscope (Leica Stellaris 5). Acquisitions were made with an XY resolution of 361.1 nm for an area of about 10 mm2 total per section ...
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bioRxiv - Microbiology 2024Quote: ... Stained dishes were visualized using an inverted confocal laser scanning microscope (CLSM) (Leica Stellaris 5, DMi8) with a 20× objective ...
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bioRxiv - Neuroscience 2024Quote: Immunofluorescent staining in the striatum was imaged with a Stellaris 5 confocal microscope platform from Leica Microsystems or a Keyence microscope using a 20X or 63X objective (1024 x1024 pixel resolution) ...
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bioRxiv - Cancer Biology 2024Quote: ... embedded in paraffin and sectioned as 5 µm thick slides using a Leica microtome (Leica Biosystems) followed by deparaffinization and rehydration (Xylol ...
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bioRxiv - Neuroscience 2024Quote: ... A CMOS camera (acA2040-90umNIR, Basler) and a ×5 0.15-NA objective (HCX PL FLUOTAR, Leica) were utilised for recording ...
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bioRxiv - Bioengineering 2024Quote: ... and the cells were imaged daily for the next 5 days with an inverted microscope (Leica DM IL LED ...
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bioRxiv - Bioengineering 2024Quote: ... and allowed to cure for 48 hours before imaging with a STELLARIS 5 confocal microscope (Leica). Image analysis of the confluence and proliferation of CECs on the gels was conducted with CellProfiler ...
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bioRxiv - Bioengineering 2024Quote: ... 5-µm sections were obtained from blocks using a Leica RM2255 microtome (Leica Biosystems, Nussloch, Germany) and mounted on microscope slides ...
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bioRxiv - Cell Biology 2024Quote: ... The experiments for Figure 5E were performed using a STELLARIS 5 confocal laser scanning microscope (Leica) equipped with an HC PL APO CS2 63x ...
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bioRxiv - Cell Biology 2024Quote: ... Embryos and egg chambers were imaged at RT on an inverted Stellaris 5 confocal microscope (Leica) with a 63x/1.4 NA oil immersion objective.
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bioRxiv - Immunology 2021Quote: ... primary antibody incubation at 1/400 dilution for 30 min then detection using the BOND™ Polymer Refine Detection System (DS9800, Leica Biosystems) as per manufacturer’s instructions.
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bioRxiv - Cell Biology 2020Quote: ... Samples were stained with phalloidin 647 and fluorescent z-stacks were acquired by confocal microscopy (Leica SP8, or Zeiss Examiner Z.1) at a pixel size of 60-70 nm ...
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bioRxiv - Bioengineering 2021Quote: ... Samples were longitudinally cut into slices with a thickness of 1 mm and observed by confocal laser scanning microscopy (CLSM, Leica TCSSP8 MP, Leica microsystems, GER). Semi-quantitative analysis was conducted through the assay of the horizontal and vertical fluorescence intensity of the hybrid layer ...
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bioRxiv - Bioengineering 2020Quote: ... Observations of the samples were carried out under fluorescence microscope (Axio Imager D.1, Zeiss) and confocal microscope (Leica SP5 upright Confocal).
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bioRxiv - Cell Biology 2021Quote: ... HMC-1 cells were loaded into membrane carriers and were high-pressure frozen in a Leica EM PACT1 (Leica microsystems, Wetzlar, Germany). T ...
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bioRxiv - Microbiology 2020Quote: ... Max projections of confocal z-stacks (25 z steps spaced 1 μm) were acquired on a SP5 resonant APD (Leica, Wetzlar, Germany) at 1.7x zoom using a 63x glycerol objective (numerical aperture 1.4).
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bioRxiv - Cell Biology 2021Quote: ... Freeze substitution was performed over 3 d at −90°C in anhydrous acetone containing 1% OsO4 and 0.1% uranyl acetate using an automatic freeze substitution machine (EM AFS, Leica Microsystems, Vienna, Austria). Epon/Araldite infiltrated samples were then embedded in a thin layer of resin and polymerized for 3 d at 60°C ...
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bioRxiv - Cancer Biology 2022Quote: ... and incubated overnight at 4°C in 0.05% Digitonin-Antibody buffer containing a rabbit anti mouse-p53 Ab (1:100 Leica, NCL-p53-CM5p). Samples incubated with rabbit IgG (1:150 ...
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bioRxiv - Neuroscience 2021Quote: Every 4th brain section was imaged as a Z-stack (1 μm step size) using either the Zeiss LSM 800 (Zen, v2.6.76) or Leica SP8 confocal microscope (Leica Application Suite X, v3.5.6.21594) at 20x magnification ...
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bioRxiv - Cancer Biology 2021Quote: ... and red tdTomato/CMDiI channels and were recorded as approximately 1 x 4 stitches at 10x magnification using a Leica sp8 confocal microscope (Leica, Wetzlar, Germany). Consecutive stitch sequences were processed into a single image using Fiji 33 using the plugin by Preibisch et al. ...
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bioRxiv - Bioengineering 2020Quote: ... eyes were fixed in 4% paraformaldehyde (w/v) in PBS for 1 hour and embedded in optimal cutting temperature compound (Leica Biosystems, Germany) and stored at -80C until cryosectioning ...
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bioRxiv - Cell Biology 2020Quote: ... Freeze substitution was performed over 3 days at –90 °C in anhydrous acetone containing 1 % OsO4 and 0.1 % uranyl acetate using an automatic freeze substitution machine (EM AFS, Leica Microsystems, Vienna, Austria) Serial semi-thick sections (300nm ...
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bioRxiv - Cell Biology 2021Quote: ... Freeze substitution was performed over 3 d at -90 °C in anhydrous acetone containing 1 % OsO4 and 0.1 % uranyl acetate using an automatic freeze substitution machine (EM AFS, Leica Microsystems, Vienna, Austria). Epon/Araldite infiltrated samples were flat embedded in a thin layer of resin ...
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bioRxiv - Molecular Biology 2023Quote: ... blotted with Whatman 1 filter paper and vitrified into liquid ethane at -180°C using a Leica GP2 plunger (Leica microsystems, Austria). Frozen grids were transferred onto a Talos 200C Electron microscope (FEI ...
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bioRxiv - Cancer Biology 2023Quote: ... Slides were immediately washed in running distilled water for 1 min prior to counterstaining cell nuclei with diluted Gill’s Hematoxylin (Leica Biosystems, Wetzlar, Germany). Slides were washed under running distilled water ...
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bioRxiv - Plant Biology 2023Quote: 50 µm-thick cross sections were prepared from apical and basal internodes of Arabidopsis inflorescence stems at stage 1 (Altamura et al., 2001) by a vibrating blade microtome (Leica VT1200 S), stained with a 0.05% (w/v ...
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bioRxiv - Cell Biology 2023Quote: ... Imaging was performed using Leica DMI8 confocal microscope and HC PL APO CS2 20x objective (excitation, 488 nm, emission, 498 nm - 550 nm, pinhole size of 1 Airy Unit) (Leica Microsystems, Germany), pixel dimensions 0.48 μm px-1 ...
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bioRxiv - Cancer Biology 2024Quote: ... The slides were heated at 60°C for 1 h in an oven before H&E staining in the autostainer (Leica Autostainer XL). IHC was performed using a detection kit (Vector Laboratories ...
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bioRxiv - Molecular Biology 2023Quote: ... blotted with Whatman 1 filter paper and vitrified into liquid ethane at –180 °C using a Leica GP2 plunger (Leica microsystems, Austria). Frozen grids were transferred onto a Talos 200C Electron microscope (FEI ...
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bioRxiv - Bioengineering 2022Quote: ... The samples were stored at −80°C for less than 1 week prior to sectioning (10 μm thick) using a CM1950 Cryostat (Leica, Wetzlar, Germany). Tissue sections were fixed and permeabilized in 1:1 methanol:acetone solution at −20°C ...
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bioRxiv - Cancer Biology 2023Quote: ... Primary antibody incubation at 1:250 dilution for 30 minutes was followed by detection using the BOND™ Polymer Refine Detection System (DS9800, Leica Biosystems) as per manufacturer’s instructions.
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bioRxiv - Bioengineering 2023Quote: ... Slides were let dry for at least 1 h prior to imaging at the microscope (Leica DM 2000 LED fitted with a Leica DFC450c camera). Ten images from different regions of the slide were acquired and the number of invaded cells counted using custom-made software further described in the next section ...
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bioRxiv - Physiology 2024Quote: ... an area of each section (1 section/mouse) was acquired at 200x magnification using a confocal laser scanning microscope (Leica TCS SP8). Sequential scanning was employed using the 488 nm laser to visualize Alexa 488 and the 405 nm UV lasers to visualize DAPI staining ...
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bioRxiv - Neuroscience 2024Quote: 20X hippocampal tiles were acquired using a confocal microscope with a 16 μm z-dimension and 1 μm step size (Leica Stellaris 8). Images were opened in Imaris image analysis software (Oxford Instruments) ...
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bioRxiv - Microbiology 2024Quote: ... we localized infection centers at this point of the protocol in reference to the gridded checkerboard by their HSV-1 mediated mChe expression using a wide-field fluorescence microscope (Leica Microsystems, Germany), and the filter set N2.1 containing the emission long pass filter LP590 which largely blocks the autofluorescence generated by the glutaraldehyde fixation ...
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bioRxiv - Neuroscience 2024Quote: ... 30μm serial sagittal sections were collected from Cx3cr1GFP/+ animals in a tempero-medial manner (1 in 12 series) using a vibratome (Leica VT 1200S) and kept as free-floating sections in ice-cold PBS ...
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bioRxiv - Molecular Biology 2024Quote: ... For scoring (graphs Figure 1) the percentage of cells expressing the chromatin markers were scored manually under an epi-fluorescence microscope (Leica DM6000 Leica microsystems, Germany).
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bioRxiv - Pathology 2024Quote: Immunohistochemistry was performed using monoclonal mouse anti-CD8 (1:150, clone 4B11, NCL-CD8-4B11, Novocastra, Leica Microsystems, Newcastle Upon Tyne, UK) on sections from paraf-fin embedded tumour samples ...