Labshake search
Citations for Leica :
51 - 100 of 768 citations for Sodium Voltage Gated Channel Alpha Subunit 10 SCN10A Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2021Quote: ... 400 Hz scan speed) and visualized using the surface channel mode of the 3D viewer module (Leica Las X software).
-
bioRxiv - Cell Biology 2021Quote: ... specific band-pass filter sets were used to prevent bleed through from one channel to the next (for GFP, L5 (Leica); for mCherry ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... and images of mtDsRed-expressing U87 and GL261 spheroids were acquired by RFP fluorescence channel using a 4x/0.1 objective with a Leica DM IL LED® (Leica) at the end point of experiments.
-
bioRxiv - Neuroscience 2022Quote: ... All data was acquired as multi-channel 16-bit images using the LAS AF imaging software suite (v. 3.5; Leica Microsystems).
-
bioRxiv - Immunology 2023Quote: ... to photo-convert PS-CFP2 from cyan to green emission and filtered the emission channel with the bandpass filters ET525/36 (Leica) or ET525/50 (Chroma) ...
-
bioRxiv - Biophysics 2023Quote: ... The tile sets were merged and maximum intensity projections for each channel were calculated using LAS X (Leica, Wetzlar, Germany). Q35-YFP was excited at 488 nm and 0.5 % total laser power and emission was detected from 509 nm to 551 nm ...
-
bioRxiv - Biochemistry 2023Quote: ... In the first channel, a 1-ml syringe tip (Braun, 9166017V) was used to connect the perfusion needle (Leica, 39471024) and the reference tube (Ismatec Reglo ...
-
bioRxiv - Cell Biology 2023Quote: ... Time-lapses of regions enriched in metaphase cells were acquired every 20 seconds in the bright-field and far-red (SiR-DNA) channels using a 20x 0.40 NA DRY NPLAN Epi objective (Leica Microsystems). When most dividing cells in the field of view reached late anaphase and telophase ...
-
bioRxiv - Bioengineering 2024Quote: ... microscope or in the transmitted light and in the RFP channel (580 nm) on a Stellaris 8 microscope using the LAS X software in both cases (Leica).
-
bioRxiv - Plant Biology 2022Quote: ... mounted in Clearsee Alpha solution and observed using a confocal TCS SP5 or TCS SP8 confocal microscope (Leica). Auramine O was excited at 488 nm and the emission was collected at 500-570 nm.
-
bioRxiv - Biochemistry 2019Quote: ... the phase-separated droplets were imaged in both the mCherry and the GFP channel by confocal fluorescence microscopy (Leica TCS SP8) with a 63x NA 1.4 oil objective (Leica) ...
-
bioRxiv - Neuroscience 2022Quote: ... The emitted light was split by a dichromatic filter at 560 nm into two channels and collected by separate multi-alkali detectors after 500–550 nm and 565–605 nm bandpass filtering (Leica Microsystems). Animals were injected through a venous catheter (acute experiments ...
-
bioRxiv - Molecular Biology 2023Quote: ... Image acquisition was performed as z-stack images of 250 μm in depth in 3 separate channels using a Leica SP8 confocal microscope (Leica, Germany).
-
bioRxiv - Immunology 2023Quote: ... Transiently transfected HEK293 cells in 8 well chamber slide were directly imaged with a Leica SP8 inverted five channels confocal microscope (Leica Microsystems). HEK293 cells ...
-
bioRxiv - Neuroscience 2021Quote: ... Immunofluorescence was acquired using a scanning sequential mode to avoid crosstalk among simultaneously scanned channels with two laser lines: Argon – laser (488 nm) and DPSS – laser (561 nm) (Leica Microsystems, Germany). Three-dimensional images were acquired over 20-μm z-axis with a 1.0 - μm step size ...
-
bioRxiv - Cancer Biology 2021Quote: ... and red tdTomato/CMDiI channels and were recorded as approximately 1 x 4 stitches at 10x magnification using a Leica sp8 confocal microscope (Leica, Wetzlar, Germany). Consecutive stitch sequences were processed into a single image using Fiji 33 using the plugin by Preibisch et al. ...
-
bioRxiv - Neuroscience 2023Quote: Dual or triple channel STED imaging of Ib and Is AZs was carried out using a Leica SP8 confocal/STED 3× microscope (Leica Microsystems, Mannheim, Germany) with 100X ...
-
bioRxiv - Bioengineering 2020Quote: U87-MG migration across the microfluidic device was monitored daily by acquiring fluorescent images of the central gel channel with a DMi8 Yokogawa W1 spinning disk confocal microscope with a Hamamatsu EM-CCD digital camera (Leica Microsystems, Buffalo Grove, IL). Three regions of interest (ROI ...
-
bioRxiv - Microbiology 2021Quote: ... pre-warmed at 37°C and the channels were imaged at x64 magnification using a DM-IRE2 confocal laser scanning microscope (Leica Microsystems Heidelberg GmbH, Germany) and the accompanying Leica Microsystems Confocal Software (version 2.61 Build 1537) ...
-
bioRxiv - Microbiology 2020Quote: ... and 15 snapshots were taken per experiment in both the DIC and DiD channels with a Leica Biosystems 6000B instrument (Leica Biosystems, Amsterdam, The Netherlands). All snapshots were analyzed for total area of fluorescent spots quantified using the ParticleAnalzer plugin of ImageJ ...
-
bioRxiv - Cancer Biology 2023Quote: Cells were seeded in 35 mm diameter dishes (Alpha Plus; Scientific Corp., Taoyuan, Taiwan) overnight and imaged with a Leica DMI6000 microscope (Leica Microsystems, Wetzlar, Germany) equipped with an HCX PL FL × 20/0.4 numerical aperture objective and an Andor Luca R EMCCD camera (Andor Technology ...
-
bioRxiv - Neuroscience 2023Quote: ... Lewy bodies were counted on sections from the described regions following immunohistochemistry for alpha-synuclein (Leica, cat# ncl-l-asyn, 1:100-400 dilution). Of note ...
-
bioRxiv - Genomics 2023Quote: ... slides were deparaffinized and automatically stained with specific antibodies against synovial cell markers (PECAM-1, DAKO JC/70a, 1:10; CD20, Ventana Roche L26, prediluted; CD3, Leica LN10 ...
-
bioRxiv - Plant Biology 2021Quote: ... Calli embedded in each sodium alginate gel were counted under a dissection microscope (M165 FC, Leica) at 2.5× magnification ...
-
bioRxiv - Neuroscience 2020Quote: ... anti-glial fibrillary acidic protein (GFAP; mouse anti-human monoclonal antibody GA5, with bond heat-induced epitope retrieval, epitope retrieval time 10 min, PA0026; Leica Biosystems, Wetzlar, Germany); anti-ionized calcium-binding adapter molecule 1 (IBA1 ...
-
bioRxiv - Neuroscience 2022Quote: ... containing the PrL cortex and NAcore were collected in 0.1M PBS containing 0.02% sodium azide with a vibrating microtome (Leica), and stored at 4ºC until processing.
-
bioRxiv - Plant Biology 2019Quote: Samples used in cell number and cell size quantifications were fixed (1% glutaraldehyde, 4% formaldehyde, 0.05M sodium phosphate, and after dehydration, embedded in Leica HistoResin ...
-
bioRxiv - Pathology 2021Quote: ... slides were heated to 100°C in sodium citrate-based ER1 buffer for 20 minutes (Leica Biosystems, Richmond, IL). Slides were then stained with anti-Iba1 antibody (1:2000 ...
-
bioRxiv - Genetics 2021Quote: ... Animals stained were mounting in agarose 2 X pads with a sodium azide drop (0,05 M) to count amyloid deposits by fluorescent microscopy (DM2500, Leica) and quantify stained area of worms by digital dissection microscope (DMD108 ...
-
bioRxiv - Plant Biology 2023Quote: ... The samples were washed with 0.05 M sodium cacodylate buffer and embedded in 4% agarose and sliced using VT1000S vibratome (Leica). For localization and auxin/cytokinin distribution pattern ...
-
bioRxiv - Cancer Biology 2020Quote: ... The following antibodies were diluted in Antibody Diluent (Leica) as indicated ...
-
bioRxiv - Cell Biology 2023Quote: ... Images were acquired using a DMI 6000B microscope with 10 × 10 magnification (Leica). The Image J software (NIH ...
-
bioRxiv - Molecular Biology 2020Quote: ... Representative whole-body images of transgenic worms were taken using 10 mM Sodium Azide for immobilization and imaged at 20x magnification using a Leica DM5500B compound scope with LAS X software (Leica).
-
bioRxiv - Molecular Biology 2022Quote: ... Adult animals were then immobilized with 1% sodium azide and GFP fluorescence was assessed using a DM6000B-Cs microscope (Leica) equipped with a DFC 365FX camera (Leica ...
-
bioRxiv - Developmental Biology 2023Quote: ... Fifty-micrometer-thick coronal sections of the brains were cut in sodium phosphate buffer (PBS 50 mM, pH7.4) using a Leica VT1000S vibratome (Leica Biosystems) and stored at -20°C in cryoprotectant until further processing ...
-
bioRxiv - Neuroscience 2024Quote: ... Spinal cord segments were stored at 4° in PBS containing 0.1% sodium azide until they were sectioned at 70 µ using a vibratome fitted with a disposable blade (Leica). Immunostaining was performed in floating sections using antibodies against CHAT (1:250 ...
-
bioRxiv - Physiology 2020Quote: ... primary antibody exposure was followed by secondary antibody (Leica Biosystems) and staining using the BondTM Polymer Refine Detection kit (DS9800 ...
-
bioRxiv - Neuroscience 2022Quote: ... It was then stored in 30% sucrose solution with 0.5% sodium azide at 4°C until preparation of 40 µm-thick coronal sections using a freezing microtome (SM 2010R, Leica Biosystems). Four to eight sections per mouse through the dorsal hippocampal region were used to evaluate hippocampal sclerosis by cresyl violet staining or gliosis by glial fibrillary acidic protein (GFAP ...
-
bioRxiv - Neuroscience 2020Quote: ... Sections (10 uM, Leica CM3050) were mounted onto electrostatically charged slides and images were collected using a Nikon TiS Microscope and associated software.
-
bioRxiv - Neuroscience 2023Quote: ... sectioned (10 μM, Leica CM3050) and mounted on electrostatically charged slides ...
-
bioRxiv - Neuroscience 2022Quote: ... 10 control and 10 MK801-treated brains were cut to 10-μm thick frozen sections with a freezing microtome (Leica CM 1860 UV, Leica, Germany). The sections were stained with hematoxylin and eosin (Sigma-Aldrich ...
-
bioRxiv - Cancer Biology 2023Quote: ... Slides were incubated in anti-GRPR antibody (SP4337P, Acris Antibodies) in Bond Primary Antibody Diluent (1/100, Cat# AR9352, Leica) overnight at 4°C and anti-Ecad (1/100 ...
-
bioRxiv - Cell Biology 2020Quote: ... The images were acquired using inverted microscope with 10 × 10 magnification (Leica DMI 6000B, Leica, Germany). Image J software (NIH ...
-
bioRxiv - Cell Biology 2020Quote: ... The images were acquired using inverted microscope with 10 × 10 magnification (Leica DMI 6000B, Leica, Germany). Image J software (NIH ...
-
bioRxiv - Cell Biology 2021Quote: ... Each field was obtained at a magnification of 10×10 (microscope Leica and digital camera Sigeta). Collagen area and total field area were determined using the ImageJ software (version 1.52a) ...
-
bioRxiv - Microbiology 2021Quote: ... fixed with 10% Neutralized formalin (Leica), blocked ...
-
bioRxiv - Pathology 2021Quote: ... Heat-induced epitope retrieval was conducted by heating slides to 95°C for 20 minutes in sodium citrate-based ER1 buffer (Leica Biosystems, Richmond, IL) before immunostaining ...
-
bioRxiv - Neuroscience 2020Quote: ... we followed 100 cells using z-stacks (10 mm scan; 10 steps) projected in 3D by Leica software to determine co-labeling (400x magnification) ...
-
bioRxiv - Cell Biology 2022Quote: ... Z-stacks (10 images, 0.2 μm) were deconvoluted (blind, 10 iterations, with the LEICA AF 2.7.3.9 software).
-
bioRxiv - Developmental Biology 2021Quote: ... Water immersion 10× objectives were used in both systems: N-Achroplan 10×/0.30 NA (Zeiss) and HC APO L 10×/0.30 NA (Leica), respectively ...