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Citations for Leica :
51 - 100 of 114 citations for Recombinant Human Membrane Metallo Endopeptidase since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2023Quote: The 5.0-µm FFPE sections for laser microdissection (LMD) were mounted onto PEN-membrane glass slides (Leica) following by deparaffinization ...
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bioRxiv - Cell Biology 2023Quote: ... tympanic membrane and ossicles were carefully removed under a stereomicroscope (Leica M165 FC, Leica Biosystems, Wetzlar, Germany). To perfuse buffer through the middle ear ...
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bioRxiv - Neuroscience 2020Quote: ... Fixed mouse brains and monkey or human brain samples were sectioned with a cryostat (model CM3050S, Leica) or a vibratome (Leica ...
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bioRxiv - Cell Biology 2020Quote: Western blot analysis for human iPSC–derived muscles was performed using antibodies to Dystrophin (NCL-DYS1, Leica), gamma-dystroglycan DAG1 (SC-165997 ...
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bioRxiv - Neuroscience 2022Quote: Paraffinized human optic nerves were cut in 5 μm sections using the microtome RM2155 (Leica, Wetzlar, Germany) and placed on 1 mm thick microscope slides (Marienfeld ...
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bioRxiv - Immunology 2023Quote: Platelet interactions with human monocytes were imaged in a Leica TCS SP5 SMD confocal system (Leica Microsystem). Cells were seeded in microslide 8-Well IBIDI chamber ...
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bioRxiv - Bioengineering 2019Quote: ... Pictures of the membranes after immersion were taken with a Leica DMI400 (Leica Microsystems, Buffalo Grove, IL, USA). The PDMS membranes were fabricated by spinning PDMS attached to a silicon wafer at 1650 rpm (resp ...
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bioRxiv - Neuroscience 2022Quote: Human and mouse brain tissue was sectioned (30 μm-thick sections) using a SM2010 R sliding microtome (Leica) equipped with a BFS-3MP freezing stage and cooling unit (Physitemp ...
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bioRxiv - Cancer Biology 2020Quote: ... The tissue blocks were sectioned by cryotome into 170 - 210 consecutive 10 µm thin tissue sections (1.7 - 2.1 mm total depth) which were placed on PEN membrane slides (Leica Microsystems). Representative sections were mounted on charged glass slides and stained with hematoxylin and eosin (H&E ...
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bioRxiv - Cell Biology 2022Quote: ... Lamella sites were chosen based on the cell membrane signal obtained from the DM6 cryo-Light microscope (Leica Microsystems) correlated with the SEM images using MAPS 3.14 software ...
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bioRxiv - Developmental Biology 2022Quote: ... 15μm brain sections were cut and adhered to PEN membrane-coated glass slides (Leica Microsystems Inc, Buffalo Grove, IL) and frozen at −80°C ...
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bioRxiv - Developmental Biology 2022Quote: Images from mouse and human samples were acquired with a Leica TCS SP8 confocal laser scanning microscope (Leica Microsystems). All images are shown as maximum intensity projections of acquired z-stacks of about one cell thickness (6-10 µm) ...
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bioRxiv - Immunology 2022Quote: ... serial human kidney sections were stained with anti-CD3 (LN10) or anti-CD8 (4B11; both Leica Biosystems, Chicago, IL) antibodies ...
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bioRxiv - Cancer Biology 2023Quote: ... automated methods on the Leica Bond Max autostainer using a mouse monoclonal anti-human CD3 antibody (Leica, clone LN10). Whole slide images of IHC stained slides were digitally captured using an Olympus IX83 microscope at 10x magnification and fixed exposure times ...
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bioRxiv - Cancer Biology 2020Quote: ... In regions with CCF two to four consecutive cryosections with a thickness of 12 µm were put on membrane-slides (Leica Frame Slides ...
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bioRxiv - Neuroscience 2020Quote: ... with one to three processes and a smooth cell membrane (Fig 2A) as visualized using an inverted microscope (DMIL; Leica) equipped with a fluorescent LED light source (X-Cite 110LED ...
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bioRxiv - Neuroscience 2021Quote: ... 20µm sections with alignment holes punched into the tissue by a glass pipet were prepared in a series with every other section aimed for in situ hybridization and collected on superfrost plus slides and the other for laser capture microdissection and collected on PEN coated membrane slides (Leica). Double fluorescence in situ hybridization (FISH ...
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bioRxiv - Biochemistry 2019Quote: 5-micron FFPE sections were mounted onto MMI membrane slides (MMI, 50102) and stained using an automated stainer (Leica XL) at the Histology Core at University of Manchester ...
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bioRxiv - Genomics 2021Quote: ... Sections of 10μm thickness were cut from the paraffine-embedded tissue blocks and mounted onto poly-ethylene naphtholate (PEN)-membrane slides (Leica). Tissue slides were stained with the following haematoxylin and eosin (H&E ...
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bioRxiv - Developmental Biology 2020Quote: ... For sequence analysis fetal tissues embedded in OCT were sectioned at 10µm onto polyethylene napthalate (PEN) membrane slides (Leica, 11505158) and heart tissue was collected using a Leica LMD7000 Laser Microdissection System from CVB3-infected fetuses showing a VSD and uninfected control fetuses ...
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bioRxiv - Neuroscience 2023Quote: ... 1 µm per z-step) of neurons expressing fluorescent membrane markers were captured using a 20×0.7 NA (Leica SP5) 20×0.75 NA (Leica Stellaris ...
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bioRxiv - Neuroscience 2023Quote: ... At day EP14 slices were cut out along with the membrane and placed into the perfusion chamber mounted on a spinning disc microscope (Leica DMI 6000B (Leica Microsystems ...
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bioRxiv - Physiology 2024Quote: ... The number of neutrophils that migrated to the lower side of the membrane was counted in 10 random microscope fields using the x20 objective (Leica). Neutrophils that passed through the membrane were quantified ...
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bioRxiv - Neuroscience 2024Quote: ... and we determined the distribution of GM1 on the lipid structures membrane using a confocal microscope (LEICA SP8 STED 3X).
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bioRxiv - Cell Biology 2019Quote: ... Immunofluorescence analyses of grafted TA muscles were performed using antibodies specific for: human lamin A/C (mouse monoclonal IgG2b, 1:400, NCL-Lam-A/C, Novocastra, Leica Biosystems ...
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bioRxiv - Cell Biology 2021Quote: Fixed and stained human pancreatic tissue sections were examined using a commercially available DragonFly200 spinning disk system (Andor) on a DMi8 microscope (Leica) with Plan Apo 40× NA 1.3 and Plan Apo 63× NA 1.4 oil immersion objectives ...
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bioRxiv - Cancer Biology 2021Quote: All staining of human tissues was performed on a Leica Bond RX automated stainer (Leica Microsystems, Inc., Buffalo Grove, IL). To ensure lectin specificity ...
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bioRxiv - Bioengineering 2022Quote: ... Bio SB) or anti-human CD163 (clone EP324, Bio SB) followed by an HRP-conjugated anti-mouse secondary antibody (Leica) and revealed with the peroxidase substrate 3 ...
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bioRxiv - Neuroscience 2023Quote: ... The entire human DRG section was imaged and merged using Leica Application Suite X (LAS-X, v.3.7, Leica Microsystems). Acquisition setting were kept consistent throughout imaging across different experimental days ...
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bioRxiv - Bioengineering 2022Quote: ... The human-distal region skin tissue was cryoprotected with 30% sucrose and continually sectioned (thickness: 50 µm) using a cryostat (Leica CM 1950 ...
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bioRxiv - Cell Biology 2021Quote: ... transferred to membrane carriers at appropriate stages and immediately cryo-immobilized using an EMPACT2 high-pressure freezer (Leica Microsystems, Vienna, Austria) equipped with a rapid transfer system (Pelletier et al. ...
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bioRxiv - Cell Biology 2021Quote: ... and xz images were acquired in the presence of the membrane dye CellMask (5 mg/ml, Life Technology) using Leica TCS SP5X confocal microscope (Leica Microsystems). The MFI of Fluo-4 in the cytoplasmic region in individual cells was measured using the NIH ImageJ software ...
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bioRxiv - Molecular Biology 2020Quote: ... Tissue specimens from two patients for each disease histotype were sectioned (8 µm) by microtome onto polyethylene naphthalate membrane slides (Leica Microsystems) and hematoxylin-eosin stained ...
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bioRxiv - Neuroscience 2019Quote: ... these inclusions were laser-cut from inverted adjacent tissue polyethylene naphthalate (PEN) membrane slides positioned on the stage of a LMD6500 (Leica Microsystems) microscope ...
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bioRxiv - Neuroscience 2023Quote: ... The removed brain is immersed for 4 minutes in iced oxygenated sucrose solution and then placed on a cellulose nitrate membrane to separate and position the hemispheres in the vibratome (Leica VT1200s) to obtain 400 µm horizontal slices (speed of 0.1 mm/s) ...
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bioRxiv - Pathology 2023Quote: ... (Supplementary table 1) were cut into 10 μm-thick sections and mounted on Leica PET-membrane slides (76463-320, Leica Microsystems). Postnatal lung tissues were considered as “healthy” after microscopic/histological analysis by a pulmonary pathologist ...
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bioRxiv - Microbiology 2023Quote: ... sectioned them at 10 µm thickness using a microtome and mounted the sections on thermoexitable POL-membranes (No. 115005191; Leica, Germany). Next ...
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bioRxiv - Cancer Biology 2023Quote: Fresh frozen tissues were placed in OCT and sections were cut at 7 μm thickness and mounted onto membrane slides (Leica, 11600288). The slides were fixed in ice-cold 70% EtOH for 5 min ...
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bioRxiv - Immunology 2023Quote: ... from Apoe-/-LysMCre and Apoe-/- LysMCreCnr1flox/flox male mice collected after 4 weeks WD were mounted on RNase-free membrane metal frame slides (POL FrameSlide, Leica Microsystems). Following deparaffinization under RNase-free conditions ...
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bioRxiv - Microbiology 2024Quote: ... Part of the polycarbonate membranes with cells was dehydrated and clarified with xylene by an automaton (TP1020, Leica Biosystems, Heidelberg, Germany). Cell preparations were manually embedded in paraffin so that sagittal sections could be obtained ...
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bioRxiv - Microbiology 2024Quote: ... The paraffin blocks containing the polycarbonate membranes surmounted by the cell monolayer were cut with a Histocore Rotary microtome (Leica Biosystems). The sections were then deparaffinised ...
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bioRxiv - Neuroscience 2019Quote: ... 5μm formalin fixed paraffin embedded tissue sections of human frontal cortex were deparaffinized and processed using heat induced epitope retrieval with an EDTA-based buffer (Leica #AR9640) for 20 minutes and incubated with primary antibody for 30 minutes at room temperature ...
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bioRxiv - Cell Biology 2021Quote: Cryo-samples prepared from single-tagged CLTA-GFP expressing SK-MEL-2 cells and incubated with Alexa Fluor® 647 ChromPure human transferrin (Jackson Immuno Research Inc.) were imaged on a Leica EM Cryo CLEM system (Leica). The system consists of a Leica DM6 FS widefield microscope that is equipped with a motorized Leica EM Cryo stage and a short working distance (<0.28 mm ...
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bioRxiv - Cancer Biology 2023Quote: ... Primary antibody used for Dp427 was the anti-human Dystrophin form Leica Biosystems (1:25, Dy4/6D3, NCL-DYS1, RRID:AB_442080, Leica Biosystems, Wetzlar, Germany) and the Dystrophin antibody from Abcam (1:100 ...
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bioRxiv - Physiology 2024Quote: Human cells (A549 and BEAS-2B) were investigated using the TCS Sp5 Inverted Confocal laser scanning microscope (Leica Microsystems, Wetzlar, Germany) and a 20x /0.70 HC Plan Apochromat ...
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bioRxiv - Cell Biology 2021Quote: ... HMC-1 cells were loaded into membrane carriers and were high-pressure frozen in a Leica EM PACT1 (Leica microsystems, Wetzlar, Germany). T ...
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bioRxiv - Developmental Biology 2019Quote: ... and cryosectioned at 20 μm thickness onto either Superfrost Plus glass slides (Fisher 12-550-15) (for staining optimization) or PEN membrane slides (Fisher/Leica No. 11505158) (for LCM) ...
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bioRxiv - Molecular Biology 2023Quote: ... Tissue was stored in −80 °C and sectioned from an OCT block at 12 µm thickness and mounted on Leica PPS-membrane Laser Microdissection (LMD) slides (Leica, Cat# 11505268). A rapid stain protocol23 prepared slides for LMD ...
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bioRxiv - Microbiology 2023Quote: ... The dehydrated hNEC transwell membrane was then separated using a surgical blade and incubated in 65 °C paraffin (Leica EM-400 3801320) for 30 mins ...
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bioRxiv - Bioengineering 2023Quote: ... 10 images from different regions of the membrane were acquired with a fluorescence microscopy setup (Leica DM 2000 LED fitted with a Leica DFC450c camera).