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Citations for Leica :
51 - 100 of 1456 citations for R 4 5 Isopropylidene 2 pentanoic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... Ultrathin sections obtained using an Ultracut R ultramicrotome (Leica) were stained with uranyl acetate and lead citrate ...
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bioRxiv - Neuroscience 2021Quote: ... brains were cut in a microtome (Leica SM2000 R). All sections were stored at -20°C in a cryoprotective solution containing 1% DMSO (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2022Quote: ... Sigma-Aldrich R-2751) under binocular microscope (Leica EZ4).
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bioRxiv - Cell Biology 2024Quote: ... samples were freeze-substituted at -90°C in anhydrous acetone containing 2% osmium tetroxide and 0.1% uranyl acetate at -90°C for 72 hours and warmed to room temperature with a gradient of +5°C per hour (EM AFS-2, Leica). Sections of the samples were analysed in a Tecnai Spirit T12 Electron microscope equipped with an Eagle OCD camera (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2022Quote: ... Dried samples were then rotary shadowed with 2 nm of platinum and 4-6 nm of carbon using the ACE600 (Leica Microsystems, Germany). Areas of interest from the resulting platinum replicas were separated from glass with 5% hydrofluoric acid and deposited on carbon-coated EM grids (200 mesh formvar/carbon ...
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bioRxiv - Cell Biology 2022Quote: ... Dried samples were then rotary shadowed with 2 nm of platinum (sputtering) and 4-6 nm of carbon (carbon thread evaporation) using an ACE600 metal coater (Leica Microsystems, Germany). The resultant platinum replica was floated off the glass with hydrofluoric acid (5%) ...
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bioRxiv - Microbiology 2024Quote: ... containing 4‘,6-diamidino-2-phenylindole (DAPI) and imaged as midsections by confocal laser scanning microscopy (Leica SP8: Leica Microsystems, Wetzlar, Germany). To prevent cross talk between the channels for the different fluorochromes ...
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bioRxiv - Cell Biology 2023Quote: ... Dried samples were then rotary shadowed with 2 nm of platinum (sputtering) and 4-6 nm of carbon (carbon thread evaporation) using an ACE600 metal coater (Leica Microsystems, Germany). The resultant platinum replica was floated off the glass with hydrofluoric acid (5%) ...
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bioRxiv - Cell Biology 2023Quote: ... 30% sucrose in PBS (2:1 v/v) over-night (O/N) at 4°C with gentle shaking and frozen in OCT (Leica Surgipath, FSC22), using plastic molds (Leica Surgipath ...
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bioRxiv - Cell Biology 2020Quote: ... Dried samples were then rotary-shadowed with 2 nm of platinum and 5-8 nm of carbon using an ACE600 high vacuum metal coater (Leica Microsystems). Platinum replicas were floated off the glass by 5% hydrofluoric acid ...
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bioRxiv - Cell Biology 2020Quote: ... cells were loaded with fura-2/AM in the dark (5 μM; 30 min; RT; S1) and imaged with an upright microscope (Leica DMI6000FS, Leica Microsystems) equipped for ratiometric live-cell imaging with a 150W xenon arc lamp ...
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bioRxiv - Neuroscience 2024Quote: Mice were anesthetized using vaporized isoflurane (5% for induction, 1.5% for maintenance) and placed in a stereotactic frame (Leica, Angle 2). Opthalmic ointment was applied and rectal temperature and respiration rate were monitored throughout surgery ...
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bioRxiv - Systems Biology 2021Quote: Leica LMD 7 cutting accuracy (Leica R&D, patent EP1276586)
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bioRxiv - Developmental Biology 2022Quote: ... Semi-thin sections taken in an ultramicrotome (Leica Ultracut R) were stained with toluidine blue ...
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bioRxiv - Bioengineering 2024Quote: ... and then sectioned on a microtome (SM 2010 R, Leica) at a 60-micron thickness ...
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bioRxiv - Bioengineering 2023Quote: ... and then sectioned on a microtome (SM 2010 R, Leica) at a 90-micron thickness ...
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bioRxiv - Pathology 2024Quote: ... Tissues were embedded in Formula R paraffin wax (Leica, Germany) and sectioned at 4.5 – 5 µm using a Leica RM2235 microtome and mounted on glass Superfrost® Plus microscope slides (ThermoScientific ...
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bioRxiv - Biophysics 2022Quote: ... installed on an Ultracut R microtome (Leica Microsystems SAS, Nanterre, France). These sections were stained using Richardson’s azur II and methylene blue (Richardson et al. ...
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bioRxiv - Neuroscience 2023Quote: Brains were coronally sliced on a sliding microtome (SM2000 R, Leica) or a cryostat (CM1800 ...
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bioRxiv - Neuroscience 2023Quote: ... and finally cut frozen on a sliding microtome (Leica SM2010 R) at 40 µm ...
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bioRxiv - Zoology 2024Quote: ... and a Leica DM R fluorescence microscope (Leica Microsystems GmbH, Germany). Images were analyzed using Fiji 2.0.0.
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bioRxiv - Cell Biology 2021Quote: ... stained with DAPI (4’,6-diamidino-2-phenylindole) for visualisation of the nuclei and analysed by confocal microscopy using an inverted microscope (Leica DMI6000, objective lens 100×).
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bioRxiv - Cancer Biology 2024Quote: Cells were seeded on glass bottom dishes then image acquisitions were performed every 2 min for 4 using LiveSR spinning-disk microscope (Leica, Leica Microsystems GmbH, Wetzlar, Germany) with environmental control at 37°C and CO2 provider ...
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bioRxiv - Neuroscience 2024Quote: ... Sagittal 300-μm-thick slices were cut from the dorsal level of the hippocampus48 at 2-4 mm from midline using a vibratome (Leica VT-1200, Nussloch, Germany). Slices were stored in a reserve chamber containing slicing solution at 34°C for at least 30 min and subsequently at room temperature (20 – 25°C).
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bioRxiv - Cell Biology 2020Quote: ... cells were loaded with fura-2/AM in the dark (5 μM; 30 min; RT; S1) and imaged with an upright microscope (Leica DMI6000FS, Leica Microsystems) equipped for ratiometric live-cell imaging with a 150W xenon arc lamp ...
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bioRxiv - Cell Biology 2022Quote: ... and the cells were seeded at 2000 cells/ml (total volume 2 ml) before starting the experiment at 37ºC supplemented with 5% CO2 in a live cell chamber (Leica DMI6000B with PEKON stage). Cells that attached and spread during the first two hours were used to quantify the changes in traction stresses during adhesion ...
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bioRxiv - Bioengineering 2022Quote: ... The homogeneity of BMP-2 loading inside the film-coated 3D mini-scaffolds was assessed using a BMP-2 loading solution containing 5% of BMP-2Rhod and a fluorescence macroscope (Macrofluo Z16 Apo, Leica Microsystems, Wetzlar, Germany) with a 0.8X objective ...
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bioRxiv - Neuroscience 2023Quote: ... Stellaris 5 (Leica), or LSM 900 (Zeiss ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were then moved to 4°C for 5 min and immediately imaged using a confocal microscope (Leica SPX5 with a 63X 1.4 N.A. oil objective). Internalization of antigen was quantified by determining the percentages of cells with intracellularly-located AF488-Fab’ goat-anti-mouse IgM+G puncta in each field and by measuring the AF488 FI associated with intracellular puncta in individual cells ...
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bioRxiv - Cancer Biology 2020Quote: ... before peroxidase block was performed using an Intense R kit (Leica, DS9263). Caspase 3 was stained at a previously optimised dilution of 1/500 and Ki67 at 1/100 before washing with Leica wash buffer and then application of rabbit envision secondary (Agligent ...
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bioRxiv - Neuroscience 2022Quote: ... 30 μm brain sections were cut in a microtome (Leica SM2000 R). Sections were stored at -20 °C in a cryoprotective solution containing 1% DMSO (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2022Quote: ... before peroxidase block was performed using an Intense R kit (DS9263, Leica) for 5 minutes ...
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bioRxiv - Cell Biology 2020Quote: ... Ultrathin sections were prepared with a Reichert Ultracut R Ultramicrotome (Leica Biosystems) and mounted on formvar-coated slot grids (Agar Scientific) ...
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bioRxiv - Cell Biology 2022Quote: ... before peroxidase block was performed using an Intense R kit (DS9263, Leica). FFPE sections were rinsed with wash buffer and Podxl antibody applied for 30 minutes (Abcam ...
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bioRxiv - Cancer Biology 2024Quote: ... before peroxidase block was performed using an Intense R kit (DS9263, Leica) for 5 min ...
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bioRxiv - Cancer Biology 2023Quote: ... before peroxidase block was performed using an Intense R kit (DS9263, Leica). Sections for p21 had blocking solution applied from the Rat ImmPRESS kit (MP-7404 ...
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bioRxiv - Cancer Biology 2024Quote: ... before peroxidase block was performed using an Intense R kit (DS9263, Leica). MDA sections had an additional Mouse Ig blocking step (MKB-2213 ...
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bioRxiv - Genomics 2024Quote: ... The nuclei were differentiated with mild acid treatment (Leica Biosystems 3803598), followed by “bluing” through exposure to a weakly alkaline solution (Leica Biosystems 3802918 ...
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bioRxiv - Neuroscience 2021Quote: ... and coronal sections were cut frozen on a sliding microtome (Leica SM2010 R) to a thickness of 40 μm ...
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bioRxiv - Neuroscience 2021Quote: ... Chromogenic detection was performed with the Bond Intense R Detection System (Leica, DS9263) or the DCS Supervision 2 Polymer System (Innovative Diagnostik-Systeme ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were sectioned by a cryostat at −20°C (Leica, Germany, SM2010 R), and 30 μm coronal slices were made ...
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bioRxiv - Neuroscience 2024Quote: ... Coronal sections of 30 μm thickness were obtained in microtome (Leica SM2010 R).
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bioRxiv - Neuroscience 2024Quote: ... myoinositol (3) and ascorbic acid (0.5) using a vibratome (Leica VT1200, Germany). Brains slices were transferred to an interface type holding chamber filled with 28 °C carbogen saturated ASCF containing (in mM) ...
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bioRxiv - Neuroscience 2021Quote: ... Tissue was re-sectioned at 60 μm using a freezing microtome (Leica, SM2010 R). Next ...
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bioRxiv - Neuroscience 2021Quote: ... the brains were sliced at 50 microns using the freezing microtome (Leica, SM2010 R). Selected slices (1 in 5 serially ...
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bioRxiv - Physiology 2024Quote: ... Ultrathin sections (∼50 nm) were cut using a Leica Ultracut R microtome (Leica Microsystem) with a Diatome diamond knife (Diatome Ltd. ...
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bioRxiv - Cancer Biology 2024Quote: ... before endogenous peroxidase blocking using a BOND Intense R Detection kit (#DS9263, Leica Biomarkers) for 5 min ...
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bioRxiv - Cell Biology 2024Quote: ... In vivo retinal thickness was measured using the Envisu-R SD-OCT imager (Leica Microsystems Inc. ...
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bioRxiv - Neuroscience 2024Quote: ... Forty µm coronal sections were collected on a freezing microtome (Leica SM 2000 R) and stored in 1xPBS with 0.01% sodium azide at 4°C until processing for histological assessment.
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bioRxiv - Pathology 2024Quote: ... immersed in xylene and then transferred to molten Formula R paraffin wax (Leica, Germany). All viscera were processed using protocol 1 ...