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Citations for Leica :
51 - 100 of 997 citations for Primary Bovine Aortic Endothelial Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... Coronal 25-μm thick sections containing the primary somatosensorial cortex were cut with a vibratome (Leica VT 1000S) and stored at −20°C in 30% ethylene glycol ...
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bioRxiv - Molecular Biology 2022Quote: ... 0.3-0.5cm of primary root tip and 1.5-3cm of LRP zone were collected and cut into 40µm by Leica VT1200S fully automated vibrating blade microtome (Leica ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibody incubations were followed by signal detection using BOND Polymer Refine Detection Kit (Leica Biosystems, IL, USA) with Mayer’s hematoxylin nuclear counter stain as per manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... Primary antibody binding is detected and visualized using the Leica Bond Polymer Refine Detection Kit (Leica Biosystems DS9800) with DAB chromogen and Hematoxylin counterstain ...
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Acinar-to-ductal metaplasia in the pancreas requires a glycolytic switch and functional mitochondriabioRxiv - Cancer Biology 2022Quote: ... for 30 or 40 minutes and primary antibody binding was visualized using a Polymer Refine Detection Kit (Leica). Slides were scanned with an AT2 (Leica ...
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bioRxiv - Neuroscience 2023Quote: Fixed and stained primary hippocampal neurons were imaged at a Leica SP8 confocal microscope (Leica microsystems, Mannheim, Germany). The microscope was controlled by Leica Application Suite X (LASX ...
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bioRxiv - Neuroscience 2023Quote: ... Coronal brain sections (250 μm) containing the primary motor cortex were prepared using a vibratome (VT1200s, Leica, RRID:SCR_018453) in the same sucrose-based solution that was maintained at ∼4 °C using a recirculating chiller (FL300 ...
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bioRxiv - Immunology 2023Quote: ... Detection of the second primary antibody was performed using the Bond Polymer Red Refine Kit (DS9390, Leica Biosystems). Slides were counterstained ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibody binding was detected and visualized using the Leica Bond Polymer Refine Detection Kit (Leica Biosystems DS9800) with DAB chromogen and hematoxylin counterstain.
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bioRxiv - Genomics 2024Quote: ... Primary antibody binding was detected and visualized using the Leica Bond Polymer Refine Detection Kit (Leica Biosystems DS9800) with DAB chromogen and Hematoxylin counterstain ...
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bioRxiv - Microbiology 2022Quote: ... Cell pellets were transferred to specimen carriers and immediately frozen in the presence of 20% w/v bovine serum albumin (BSA) solution using a high-pressure freezer Leica EM ICE (Leica Microsystems, Austria). Freeze substitution was performed in the presence of 2% osmium tetroxide diluted in 100% acetone at −90 °C ...
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bioRxiv - Cell Biology 2020Quote: ... 300 μm thick parasagittal brain sections containing the primary somatosensory cortex were cut on a vibrotome (1200S, Leica Microsystems). Following a recovery period at 35 °C for 35–45 minutes slices were stored at room temperature in the ACSF ...
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bioRxiv - Neuroscience 2019Quote: Confocal microscopy of fixed primary cultures was performed at a Leica TCS SP5 confocal microscope (Leica microsystems, Manheim, Germany). The microscope was controlled by Leica Application Suite Advanced Fluorescence software ...
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bioRxiv - Neuroscience 2019Quote: ... 350-μm-thick coronal slices of the primary visual cortex (V1) were sectioned using a vibrating tissue slicer (Leica VT 1200S ...
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bioRxiv - Cell Biology 2023Quote: ... Coverslips were incubated overnight at 4°C in primary antibodies targeted against p53 protein (CM5) (Leica Biosystems; 1:300) and GFP (Abcam #ab13970 ...
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bioRxiv - Cancer Biology 2021Quote: ... and the following primary and secondary antibodies were used: rabbit anti-p53 (Leica Biosystems, NCL-L-p53-CM5p, 1:5000), rabbit anti-Mettl3 (Abclonal ...
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bioRxiv - Developmental Biology 2021Quote: Vitrified fractured primary polyp specimen were transferred from the SEM under a cryogenic temperature via VCT (VCT 100; Leica Microsystems) to the freeze-fracture device (BAF 060 ...
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bioRxiv - Synthetic Biology 2020Quote: Primary transformants growing on 0.5x Gamborg plates with antibiotic were screened using a stereo microscope with fluorescence (Leica M205 FA). Ten days after transformation and 20 plants were transferred onto new selection plates ...
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bioRxiv - Developmental Biology 2019Quote: ... Palatal tissues at the border between the primary and the secondary palate were dissected from the sample sections using a manual laser-capture microdissection system (LMD6500, Leica) and collected into tubes.
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bioRxiv - Cancer Biology 2021Quote: ... were selected from hematoxylin-stained images reflecting histopathologic regions containing either primary or metastatic tumor foci via analysis in ImageScope (Leica). Digitally-acquired images were registered in MatLab (MathWorks ...
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bioRxiv - Plant Biology 2020Quote: Primary transformants were identified using the seed-specific RFP reporter under a Leica MZ-FL III stereomicroscope (Leica Camera AG). Genotyping of CRISPR/Cas9-based mutations and T-DNA insertions were performed using KAPA-Taq (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2021Quote: ... Primary antibodies were incubated for 60 minutes and detected using the BOND™ Polymer Refine Detection System (DS9800, Leica Biosystems) as per manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... tissues were washed and incubated for 1h RT with the primary antibody diluted in IHC Antibody Diluent (Novocastra, Leica RE7133). Subsequently ...
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bioRxiv - Cancer Biology 2022Quote: ... Primary antibodies were incubated for 30 minutes and detected using the BOND™ Polymer Refine Detection System (DS9800, Leica Biosystems) as per manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Slides were incubated in anti-GRPR antibody (SP4337P, Acris Antibodies) in Bond Primary Antibody Diluent (1/100, Cat# AR9352, Leica) overnight at 4°C and anti-Ecad (1/100 ...
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bioRxiv - Physiology 2023Quote: ... Opaque yolk in three primary follicles of one ovary was measured lengthwise using an ocular micrometer on a dissecting microscope (Leica).
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bioRxiv - Immunology 2023Quote: ... The following primary antibodies were used at the consequence and with indicated dilutions and CF dyes: CD20 (Leica Biosystems PA0200), 1:1 with CF488 ...
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bioRxiv - Biochemistry 2020Quote: ... Frozen bovine lenses were sectioned to 10 μm thickness equatorially or axially using a CM 3050 Cryostat (LEICA CM 3050S, Leica Microsystems Inc., Bannockburn, IL). Sections were thaw-mounted on Indium-Tin-Oxide (ITO ...
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bioRxiv - Cell Biology 2020Quote: ... The antibodies were washed in TBS three times for three minutes at a time before the Novolink post primary block (Leica biosystems) was applied for 30 minutes at room temperature ...
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bioRxiv - Immunology 2021Quote: Primary (1ry) Antibodies were incubated for one hour and detected using the BOND™ Polymer Refine Detection System (DS9800, Leica Biosystems) as per manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... primary antibody was diluted 1:10 in the peptide solution directly.) The primary antibodies were detected by 30 minute incubation with HRP-labeled anti-mouse secondary antibody (catalog# PV6114, Leica Microsystems) followed by detection with 3,3′-Diaminobenzidine (catalog# D4293 ...
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bioRxiv - Neuroscience 2021Quote: ... Blocks containing the primary somatosensory cortex and dorsal hippocampi were dissected and coronal sections were prepared on a vibratome (VT1200S, Leica, Germany) at 50 μm thickness for immunofluorescent experiments and electron microscopy ...
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bioRxiv - Pathology 2022Quote: ... slides were stained and processed using the 17B4 primary antibody on a Leica BOND-III autostainer (Leica Biosystems, Buffalo Grove, IL).
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bioRxiv - Bioengineering 2021Quote: ... Sections were incubated (30 min, room temperature (rt)) with an anti-GFP antibody (see Table S2) in Leica Primary Antibody Diluent (Leica Biosystems). Bond Polymer Refine Detection (Leica Biosystems ...
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bioRxiv - Neuroscience 2019Quote: ... Blocks containing the primary somatosensory cortex and dorsal hippocampi were dissected and coronal sections were prepared on a vibratome (VT1200S, Leica, Germany) at 20 µm thickness for STORM experiments ...
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bioRxiv - Physiology 2020Quote: ... after which they were incubated with antibodies against GPT (AP7468b, Abgent; 1:200) and GPT2 (PAD854Mu01, Cloud-Clone Inc.; 1:200) antigens in BONDTM primary antibody diluent (AR9352, Leica Biosystems). For skeletal muscle fiber size visualization ...
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bioRxiv - Neuroscience 2023Quote: ... Brain regions containing the primary visual cortex were cut into 50 µm thick coronal sections using a VT100S vibratome (Leica Biosystems). Sampled sections were then blocked with 10% Normal Donkey Serum (NDS ...
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bioRxiv - Cancer Biology 2023Quote: ... the slides were loaded into the Bond Rx autostainer and the post primary steps were completed using the Bond Polymer Refine Detection Kit (Leica DS9800). For Ki-67 staining ...
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bioRxiv - Cell Biology 2023Quote: ... followed by 1h incubation in the same buffer containing the primary probes and a 3 min heat incubation step in a ThermoBrite slide hybridization system (Leica Biosystems), either at 75°C or 86°C ...
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bioRxiv - Microbiology 2023Quote: ... tissue sections were incubated with the primary antibody (rabbit polyclonal anti-Influenza A virus NP [ThermoFisher Scientific, PA5-32242] diluted 1:500 in Primary Antibody diluent [Leica Biosystems]) for 30 min at ambient temperature followed by a polymer-labeled goat anti-rabbit IgG coupled with alkaline phosphatase (30 min) ...
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bioRxiv - Neuroscience 2023Quote: ... 300 μm-thick sections in correspondence to the primary somatosensory cortex (SSp-ctx, AP -1.6 ± 0.6) were cut with a vibratome VT1200S (Leica Biosystems, Wetzlar, DE) using a 0.12 mm/s cutting speed and a 1.9 mm cutting amplitude ...
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bioRxiv - Cell Biology 2023Quote: ... and immunostained using primary and secondary antibodies (listed in ESM Methods) and imaged using a TCS SP5 confocal microscope (Leica, Germany). Immunostaining of paraffin-embedded pancreas from mice with beta cell-selective deletion of Dnmt3a was performed according to [10 ...
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bioRxiv - Developmental Biology 2021Quote: ... freeze-fractured primary polyp was transferred for cryo-SEM imaging using a vacuum cryo-transfer device (VCT 100, Leica Micro-systems, Vienna, Austria) and loaded into the SEM where it was imaged at -120 °C.
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bioRxiv - Neuroscience 2019Quote: ... 350-μm-thick coronal slices of the primary visual cortex (V1) were sectioned using a vibrating tissue slicer (Leica VT 1200S, Leica Microsystems, Wetzlar, Germany). The slices were then transferred to a suspended mesh within an incubation chamber filled with artificial cerebrospinal fluid (ACSF ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Glial fibrillary acid protein (GFAP) Iimmunohistochemistry was carried out using GFAP primary monoclonal antibody and the Novocastra™ and Novolink DM polymer detection system (Leica Biosystems, UK) as described previously (39–42).
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bioRxiv - Neuroscience 2023Quote: Neuron specific enolase (NSE) immunohistochemistry was carried out using NSE primary monoclonal antibody and the Novocastra™ and Novolink DM polymer detection system (Leica Biosystems, UK) as described previously [54 ...
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bioRxiv - Neuroscience 2022Quote: ... containing the primary somatosensory cortex (S1) were cut in ice-cold (4 °C) NMDG ACSF using a Leica VT1200s vibratome (Leica Biosystems, Buffalo Grove, IL) and transferred to warm (33 °C ...
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bioRxiv - Cancer Biology 2020Quote: ... Whole mount images of primary tumors and lungs were taken with a Leica MZ10 F modular stereo microscope (Leica Microsystems, Buffalo Grove, IL, USA).
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bioRxiv - Cancer Biology 2022Quote: Tissue sections were incubated for 1 hour in primary antibodies and detected using the BOND Polymer Refine Detection System (DS9800; Leica Biosystems, Buffalo Grove, IL) in accordance with the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: Tissues sections were incubated for 1 h in primary antibodies and detected using the BOND Polymer Refine Detection System (DS9800; Leica Biosystems, Buffalo Grove, IL) in accordance with the manufacturer’s instructions ...