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Citations for Leica :
51 - 100 of 1064 citations for 3RS 4 Dimethylamino 3 methyl 2 2 diphenylbutanenitrile Isodidiavalo since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... containing 4‘,6-diamidino-2-phenylindole (DAPI) and imaged as midsections by confocal laser scanning microscopy (Leica SP8: Leica Microsystems, Wetzlar, Germany). To prevent cross talk between the channels for the different fluorochromes ...
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bioRxiv - Cell Biology 2023Quote: ... 30% sucrose in PBS (2:1 v/v) over-night (O/N) at 4°C with gentle shaking and frozen in OCT (Leica Surgipath, FSC22), using plastic molds (Leica Surgipath ...
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bioRxiv - Cell Biology 2022Quote: ... Dried samples were then rotary shadowed with 2 nm of platinum and 4-6 nm of carbon using the ACE600 (Leica Microsystems, Germany). Areas of interest from the resulting platinum replicas were separated from glass with 5% hydrofluoric acid and deposited on carbon-coated EM grids (200 mesh formvar/carbon ...
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bioRxiv - Cell Biology 2022Quote: ... Dried samples were then rotary shadowed with 2 nm of platinum (sputtering) and 4-6 nm of carbon (carbon thread evaporation) using an ACE600 metal coater (Leica Microsystems, Germany). The resultant platinum replica was floated off the glass with hydrofluoric acid (5%) ...
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bioRxiv - Pathology 2021Quote: ... Slides were then treated with 3-4% hydrogen peroxide (Leica Biosystems RE7101) for 10 minutes ...
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bioRxiv - Immunology 2022Quote: ... Approximately 2 μl of each sample was processed by HPF using a Leica EM PACT 2 (Leica, Milton Keynes, UK). After HPF ...
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bioRxiv - Biochemistry 2023Quote: ... Grids were glow discharged in air for 2 min and sample (2 μL) applied in an EM GP2 plunge freezer (Leica) at 4°C and 90% humidity ...
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bioRxiv - Cancer Biology 2023Quote: ... Sections were then subjected to heat induced antigen retrieval and stained for anti-phospho-p44/42 MAPK (ERK1/2) (Thr202/Tyr204) and anti-KI-67 (CST #9027, 1/400 in PBS/2% BSA) on bond RX (LEICA). Appropriate negative and positive controls and isotype controls were included ...
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bioRxiv - Microbiology 2021Quote: ... SARS-CoV-2 Nucleoprotein: 1:2000 (Leica, AR9961) 30minutes ...
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bioRxiv - Cancer Biology 2020Quote: ... Bcl-2 (clone 3.1 mouse monoclonal antibody, Leica) was used at a 1:100 dilution with epitope retrieval solution 2 pre-treatment for 20 min.
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bioRxiv - Immunology 2023Quote: ... and BOND Epitope Retrieval Solution 2 (Leica AR9640) for MPO antibody ...
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bioRxiv - Pathology 2023Quote: ... speed 2 (Oxford) or 0.5 mm/s (Leica), and blade angle slightly below 0° (Oxford ...
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bioRxiv - Cancer Biology 2023Quote: ... using BOND Epitope Retrieval Solution 2 (Leica Biosystems). Detection was achieved using a Bond Polymer Refine Detection® DAB chromogen kit and counterstained with hematoxylin ...
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bioRxiv - Immunology 2021Quote: ... After fixation organs were stored in PBS till paraffin embedment and cut of serial sections on a microtome (2-3 µm; Leica RM 2035; Leica Instruments GmbH, Nuβloch, Germany). Sections were stained by hematoxylin and eosin (HE ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The growth culture medium was changed every 2-3 days and cell morphology was regularly checked by visual inspection with phase-contrast microscopy (Leica DMIC, Leica Microsystem GMbH, Wetzlar, Germany). When the degree of confluence reaches approximately 80 % (every 4-6 days) ...
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bioRxiv - Cell Biology 2023Quote: ... specimens were sputter coated with 3-4 nm of platinum (Leica EM ACE600). Samples were imaged using a Helios Nanolab 660 DualBeam Microscope (FEI).
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bioRxiv - Molecular Biology 2020Quote: ... the individual primordial oocyte was collected from 2-3 adjacent tissue sections to ensure that one full cell was included (Leica Microsystems, Buffalo Grove, IL, USA).
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bioRxiv - Biochemistry 2024Quote: ... and finally incubated with 3 μM MitoSOX Red for 10 min and fixed with paraformaldehyde (2%) and analyzed by fluorescence microscopy (Leica TCS SP5, Buffalo Grove, IL).
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bioRxiv - Cell Biology 2021Quote: ... stained with DAPI (4’,6-diamidino-2-phenylindole) for visualisation of the nuclei and analysed by confocal microscopy using an inverted microscope (Leica DMI6000, objective lens 100×).
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bioRxiv - Cancer Biology 2024Quote: Cells were seeded on glass bottom dishes then image acquisitions were performed every 2 min for 4 using LiveSR spinning-disk microscope (Leica, Leica Microsystems GmbH, Wetzlar, Germany) with environmental control at 37°C and CO2 provider ...
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bioRxiv - Neuroscience 2024Quote: ... Sagittal 300-μm-thick slices were cut from the dorsal level of the hippocampus48 at 2-4 mm from midline using a vibratome (Leica VT-1200, Nussloch, Germany). Slices were stored in a reserve chamber containing slicing solution at 34°C for at least 30 min and subsequently at room temperature (20 – 25°C).
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bioRxiv - Neuroscience 2024Quote: ... were prepared from Sprague Dawley rats (n=3; 3-4 weeks old; Janvier Laboratories) and cut using a vibratome (Leica VT1200S) in an ice-cold oxygenated ...
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bioRxiv - Microbiology 2023Quote: ... Samples for milling were frozen on 2/2 200 gold mesh grids by plunge freezing in Leica EM GP2 or GP1 plunge freezer (Leica Microsystems, Germany) at 10°C and 90% relative humidity ...
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bioRxiv - Microbiology 2024Quote: ... followed by fine trimming of an initial region of interest (ROI) spanning 2 x 2 x 1 mm using a glass knife and Leica UC7 ultramicrotome (Leica Microsystems, Austria). The trimmed block was then mounted on an ultramicrotome stub using conductive silver epoxy (SPI ...
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bioRxiv - Developmental Biology 2022Quote: ... using a 2-photon Leica SP8 confocal microscope (Leica).
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bioRxiv - Cancer Biology 2021Quote: ... mouse anti-MUC2 (1:50, Leica, NCL-MUC-2), mouse anti-CK17 (1:10 ...
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bioRxiv - Neuroscience 2021Quote: ... and 2 CaCl2) using a vibratome (VT 1200, LEICA) and afterwards incubated in ACSF at 36 °C for 45 min ...
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bioRxiv - Plant Biology 2021Quote: ... A confocal laser scanning microscope (TCS SP5 2; Leica) and the water immersion objective lens Leica HCX IRAPO L25×/0.95W were used to visualise YFP fluorescence (excitation ...
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bioRxiv - Cell Biology 2020Quote: ... 2) Leica SP8 FALCON inverted confocal system (Leica Microsystems) equipped with a HC PL APO 63x/1.40 oil immersion lens and a temperature-controlled hood maintained at 37°C and 5% CO2 ...
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bioRxiv - Immunology 2021Quote: ... with Epitope Retrieval (ER) Solution 2 (AR9640, Leica Biosystems) was performed before each 1ry antibody was applied ...
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bioRxiv - Systems Biology 2023Quote: ... 2) Opal 520/CD61 (Ready-to-use, 2F2, Leica), 3 ...
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bioRxiv - Cancer Biology 2023Quote: ... 2 µm sections were obtained using microtome (Leica RM2245) picked randomly to represent different parts of the tumor.
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bioRxiv - Cancer Biology 2022Quote: ... with Epitope Retrieval (ER) Solution 2 (AR9640, Leica Biosystems) was performed before each primary antibody was applied ...
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bioRxiv - Cancer Biology 2021Quote: ... After heat-induced epitope retrieval (HIER) at 100°C (BOND Epitope Retrieval Solution 1 or 2, Leica, Germany; HIER 1 AR9961, HIER 2 AR9640), endogenous peroxidase activity was blocked by incubation with 3% peroxide block for 20 minutes (BOND Polymer Refine Detection System ...
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bioRxiv - Neuroscience 2020Quote: ... and 2 CaCl2) using a vibratome (Vibracut, Leica Biosystems, Germany) followed by incubation in carbogenized aCSF for 30 min at 36°C and then kept at room temperature (~21° C ...
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bioRxiv - Plant Biology 2021Quote: ... An upright confocal laser scanning microscope (TCS SP5 2; Leica) using a 40x apochromatic water immersion lens was used to visualized YFP fluorescence (excitation ...
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bioRxiv - Microbiology 2021Quote: ... and 2% water with an AFS (Leica microsystems, Vienna, Austria). After bringing the cells to RT they were washed in acetone and gradually infiltrated with epoxy resin (Agar Scientific ...
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bioRxiv - Developmental Biology 2021Quote: ... 2 mm sections were obtained using a vibratome (Leica, VT1200S) and immobilized using glue in a polystyrene-bottom dish (TPP ...
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bioRxiv - Biophysics 2023Quote: ... 2 mm sections were obtained using a vibratome (Leica, VT1200S) and immobilized using tissue seal (histoacryl blue ...
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bioRxiv - Immunology 2024Quote: ... Images were acquired with Aperio AT-2 slide scanner (Leica) and tissue lacunarity was determined by Image-J.
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bioRxiv - Physiology 2024Quote: ... The Automatic Freeze Substitution system (AFS 2, Leica Microsystems, Germany) was used with the following program ...
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bioRxiv - Microbiology 2024Quote: ... or EDTA (Bond Epitope Retrieval Solution 2, Leica; CD34, LANA) for 20 min prior to immunostaining ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... About 3-4 µm sections were prepared using an automated microtome (RM2255, Leica Biosystems, Germany) and the sections were stained with hematoxylin and eosin (H&E ...
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bioRxiv - Microbiology 2021Quote: ... as follows: SARS-CoV-2 Spike protein: 1:1000 (Leica, AR9961) 30minutes ...
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bioRxiv - Neuroscience 2021Quote: ... Slices were visualized on a resonant scanning 2-photon system (Leica) and Z-stack images were taken ...
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bioRxiv - Cancer Biology 2020Quote: ... followed by pretreatment with Epitope Retrieval Solution 2 (AR9649, Leica Biosystems) for 20 minutes and then blocking buffer for 10-20 minutes ...
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bioRxiv - Immunology 2023Quote: ... Fura-2-AM was excited using a monochromatic light source (Leica) coupled into an inverted Leica DMI4000B microscope that was equipped with a 20x objective (HCX PL Fluotar 20x ...
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bioRxiv - Cancer Biology 2023Quote: ... anti-BCL2 (Clone: BCL-2/100/D5; RTU; Leica Biosystems; #PA0117), anti-BCL6 (Clone:LN22 ...
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bioRxiv - Molecular Biology 2023Quote: ... sputter-coated with 2 nm carbon using ACE600 sputter coater (Leica).
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bioRxiv - Neuroscience 2024Quote: ... and 2 CaCl2) using a vibratome (VT 1200, LEICA, Wetzlar, Germany) and afterwards incubated in ACSF at 36 °C for 45 min ...