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Citations for Leica :
901 - 950 of 1460 citations for Carbamic acid 2 4 hexadienyl 1 1 dimethylethyl ester E E 9ci since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: Confirmation of proliferating cells and cell death were assessed by IF of Ki-67 (Leica, Cat# KI67-MM1-L-CE, 1:300) and DeadEnd Fluorometric TUNEL assay (Promega ...
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bioRxiv - Bioengineering 2024Quote: ... Slides were allowed to dry for at least 1 h prior to imaging at the microscope (Leica DM 2000 LED fitted with a Leica DFC450c camera). Ten images from different regions of the slide were acquired [35] ...
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bioRxiv - Plant Biology 2024Quote: ... a 1-μl sample was diluted in a 1:10 ratio with 10% (w/v) sucrose and observed under a stereomicroscope (Leica, M165 FC). Then ...
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bioRxiv - Genetics 2021Quote: ... Brains were post-fixed in 4% polymeric formaldehyde and sliced into 30 μm sections using a vibratome (Leica) for histological analyses ...
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bioRxiv - Developmental Biology 2021Quote: ... Formalin-fixed samples were fixed in paraffin and 4 μm sections were cut using an RM2255 microtome (Leica) and immobilized to adhesion slides (SuperFrost Plus ...
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bioRxiv - Developmental Biology 2022Quote: ... adult mouse brain tissue samples were embedded in 4% agarose and sections were cut using vibratome (Leica VT1200S). Until further processing sections were stored in ice-cold 1xPBS ...
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bioRxiv - Developmental Biology 2022Quote: ... The embryos embedded in paraffin blocks were serially sectioned at 4 μm thickness with a microtome (Leica RM2035) and placed on microscopic glass slides (Superfrost Plus ...
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bioRxiv - Immunology 2021Quote: ... Each organ was sliced into 4 µm sheets using a RM2235 manual microtome (Leica Biosystems, Buffalo Grove, IL) and imaged with a DMi1 optical microscope (Leica Biosystems ...
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bioRxiv - Neuroscience 2021Quote: ... Brainstems were embedded in 4% agarose (in PBS) and sliced coronally using a Vibratome (Leica VT1000s, Nussloch, Germany) at either 200 µm thickness for myelination analysis with CARS or 70 µm thickness for labeling oligodendrocytes.
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Formalin-fixed paraffin-embedded tissue sections (4 μm) were first deparaffinized on an automated platform (Leica Autostainer XL). After deparaffinization ...
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bioRxiv - Cell Biology 2020Quote: ... a 4 mm diameter circle was etched on the bottom of the coverslip with a diamond objective (Leica) to indicate the area of interest during sample processing for EM ...
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bioRxiv - Neuroscience 2022Quote: ... at 4°C before being sliced into 40 μm slices on a freezing microtome (Leica, Wetzlar, Germany; SM2000R). Brain sections were pre-incubated with a blocking buffer (0.5% Triton X-100 (Sigma ...
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bioRxiv - Cancer Biology 2024Quote: ... Microscopic samples were kept at 4 °C until further analyses using the Leica SP8x (Leica Microsystems Co., USA) laser scanning confocal microscope ...
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bioRxiv - Cell Biology 2024Quote: ... The tissues in paraffin blocks were subjected to sectioning (3-4 μM) by rotary type of microtome (Leica RM2255 Rotary Microtome ...
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bioRxiv - Cancer Biology 2024Quote: ... These blocks were then sectioned at a thickness of 4 µm and stained using Harris haematoxylin (Leica Surgipath) and alcoholic eosin-Y stain (Leica Surgipath) ...
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bioRxiv - Cell Biology 2023Quote: ... as described previously.4 Tissues were imaged using an SP5X laser scanning confocal microscope (Leica Microsystems CMS GmbH). Fluorescent signal distribution along gastric glands was quantified with Fiji software.
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bioRxiv - Neuroscience 2023Quote: ... frozen tissue blocks from each of the 4 AD subjects were sectioned at 10 micron thickness (Leica, CM3050s) and stored at −80°C ...
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bioRxiv - Neuroscience 2023Quote: ... it was embedded in 4% agar and sectioned into 100-µm slices using a vibraslicer (VT1200S, Leica Biosystems). The slices were mounted on glass slides with anti-fade media (#S36963 ...
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bioRxiv - Plant Biology 2023Quote: ... embedded in 4% low melting agarose and sectioned into 150 µm cross sections using the vibratome VT1000s (Leica). Images were acquired immediately after sectioning on the LSM800 using a 63.0 x oil immersion objective (Zeiss) ...
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bioRxiv - Cell Biology 2023Quote: ... They were kept at 4°C prior to image acquisition using Cell DIVE Multiplex Slide Scanner (Leica Microsystems). For all transplant experiments ...
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bioRxiv - Physiology 2024Quote: ... apparatus The tissues were embedded in paraffin (Roth) sliced in 4-5 μm sections using a microtome (Leica). The sections underwent deparaffination and rehydration procedures ...
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bioRxiv - Neuroscience 2024Quote: ... Brains were cut into 4 series of 30 µm coronal sections using a freezing microtome (Leica SM 2010R) and stored at −20°C in cryoprotectant until used ...
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bioRxiv - Plant Biology 2024Quote: ... the samples were coated with platinum (4 to 10 nm) in a EM MED020 vacuum coating system (Leica) and observed in a scanning electron microscope (ESEM Quanta 250 FEG FEI ...
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bioRxiv - Bioengineering 2024Quote: ... the samples were paraffin-embedded and cut into 4–5 μm sections using a microtome (RM2125, Leica; Germany). These sections were then stained with hematoxylin and eosin (H&E ...
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bioRxiv - Neuroscience 2021Quote: ... then sectioned coronally into 20-μm-thick slices (1:6 series of the PVN) using a Leica CM1900 cryostat (Leica Microsystems, Wetzlar, Germany). Immunofluorescence staining was performed on brain sections derived from P10 male tdTomato-Crh (Crh-IRES-Cre;Ai14 ...
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bioRxiv - Bioengineering 2022Quote: ... and stained using immunohistochemistry (IHC) for collagen I (1:4000, polyclonal rabbit, lifespan biosciences, Seattle WA) via a Bond-Max autostaining system (Leica biosystems, Lincolnshire, IL). Stained kidneys were scanned at high resolution using a CRi Panoramic SCAN 40x Whole Slide Scanner (Perkin-Elmer).
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bioRxiv - Immunology 2022Quote: ... and stained using immunohistochemistry for collagen I (1:4000, polyclonal rabbit, lifespan biosciences, Seattle WA) via a Bond-Max autostaining system (Leica biosystems, Lincolnshire, IL). Stained kidneys were scanned at high resolution using a CRi Panoramic SCAN 40x Whole Slide Scanner (Perkin-Elmer).
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bioRxiv - Plant Biology 2020Quote: ... Thick sections (1 µm) of long shoots were cut with glass knives using an Ultracut R microtome (Leica Microsystems, Buffalo Grove, IL, USA) and stained with toluidine blue ...
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bioRxiv - Immunology 2021Quote: ... z-stacks were generated with a 20x objective at a z-step of 1 µm using a SP8 laser scanning confocal microscope (Leica Biosystems, Nußloch, Germany). Composite images were generated using ImageJ with Fiji distribution [31].
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bioRxiv - Neuroscience 2021Quote: ... isogenic samples reared in the same environment were highpressure frozen (Leica HPM100 for datasets 1-5 and Leica ICE for datasets 6-8) at different stages of post-embryonic development ...
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bioRxiv - Immunology 2022Quote: ... and stained using immunohistochemistry (IHC) for collagen I (1:4000, polyclonal rabbit, lifespan biosciences, Seattle WA) via a Bond-Max autostaining system (Leica biosystems, Lincolnshire, IL). Stained kidneys were scanned at high resolution using a CRi Panoramic SCAN 40x Whole Slide Scanner (Perkin-Elmer).
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bioRxiv - Pathology 2021Quote: ... Slides were then stained with anti-Iba1 antibody (1:2000; Wako; 019- 19741 Richmond, VA) using the Bond Polymer Refine Red Kit (cat. DS9390 Leica Biosystem, Richmond, IL). The slides were stained using a pan-cytokeratin antibody (1:1000 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... RNAscope staining was followed by the C219 IHC at a 1:200 dilution for 30 minutes on a Bond RX auto-stainer using the Bond Polymer Refine Kit (Leica, Buffalo Grove, IL) minus DAB & Hematoxylin ...
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bioRxiv - Neuroscience 2021Quote: ... Regularly spaced brain series (5 × 1-in-5) of 30 μm-thick frozen sections were cut in freezing microtome (SM2000R, Leica, Wetzlar, HE, Germany) in the coronal plane ...
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bioRxiv - Neuroscience 2021Quote: images were obtained using the Axio Imager 1 microscope (Zeiss, Oberkochen, Germany) and confocal images were obtained with a laser scanning confocal microscope (Leica SPE, Mannheim, Germany) associated with a Leica LAS AF interface ...
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bioRxiv - Cell Biology 2020Quote: ... samples for the projects as listed in Table 1 were cryo-immobilized by using the following high-pressure freezers: EMPACT2+RTS (Leica Microsystems, Vienna, Austria), Leica EM ICE (Leica Microsystems ...
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bioRxiv - Neuroscience 2022Quote: ... Brains were then frozen and coronally sectioned at a thickness of 30 μm (1:5 series) using a Leica CM1900 cryostat (Leica Microsystems, Wetzlar, Germany). Fos2A-iCreER+/-::Ai14+/- sections were mounted on gelatin-coated slides and coverslipped with Vectashield containing DAPI (cat ...
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bioRxiv - Microbiology 2023Quote: ... Z stacks of horizontal plane images were acquired in 1 μm steps using a Leica SP8 AOBS inverted laser scanning microscope (CLSM, LEICA Microsystems, Wetzlar, Germany) at the INRAE MIMA2 platform (33) ...
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bioRxiv - Physiology 2023Quote: ... ten H&E stained images of 1 mm2 (4,000,000 pixel2) were randomly extracted from the entire adipose tissue image using Aperio ImageScope (Leica Biosystems Imaging, Deer Park, IL) and analyzed with the MorphoLibJ collection of mathematical morphology methods and plugins using the image processing package FIJI [36] ...
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bioRxiv - Neuroscience 2023Quote: ... P14-P16 and P20-P21 pups of both sexes were placed in a perfused chamber at room temperature (22-25°C) in oxygenated Mg2+ containing ACSF (ACSF-1) medium under a Leica DMLFS microscope (Leica Microsystems Inc., USA). The recordings were performed using a coated nichrome electrode with an Iso-DAM8A World Precision Instruments amplifier (World Precision Instruments ...
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bioRxiv - Microbiology 2023Quote: ... Z-stacks of horizontal plane images were acquired in 1 μm steps using a Leica SP8 AOBS inverted laser scanning microscope (CLSM, LEICA Microsystems, Wetzlar, Germany) at the INRAE MIMA2 platform (doi.org/10.15454/1.5572348210007727E12)54.54 ...
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bioRxiv - Cell Biology 2023Quote: ... On day 1 after seeding the grids were incubated with 10 nm colloidal gold particles for 1 minute and rapidly frozen in liquid ethane using GP2 plunge-freezer (Leica Microsystems, Vienna, Austria). Tilt series of vitrified samples were collected using a Tecnai F20 microscope (FEI Company ...
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bioRxiv - Neuroscience 2024Quote: ... together with Opal 520/570/690 Fluorophore Reagent pack detection (1:1000, Cat #FP1487001KT/FP1488001KT/FP1497001KT) on the Leica BOND RX Fully Automated Research Stainer (Leica, Buffalo Grove, IL) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... immersed in 1-Hexadecene as cryoprotectant and immediately fixed by high pressure freezing (HPF) using the Leica EM ICE (Leica Microsystems GmbH, Germany). Freeze substitution and embedding of the HPF-fixed samples were conducted in a temperature-controlled device ...
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bioRxiv - Bioengineering 2024Quote: ... 7A) were performed under an OPMI 1 FR PRO Surgical microscope (Zeiss, Jena, Germany) or Leica S9D Stereo microscope (Leica Microsystems, Wetzlar, Germany). A 30G disposable needle was used to puncture the sclera near the limbus ...
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bioRxiv - Cancer Biology 2024Quote: ... in TBST buffer for IF and subjected to staining with the following antibodies overnight at 4C: p53 (Leica, P53-CM5P-L 1:100), GFP/YFP (Abcam ...
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bioRxiv - Cancer Biology 2024Quote: ... Primary antibody (dilution at 1: 100) was incubated for 15 min at room temperature and detected using the Bond Polymer Refine Detection kit (Leica Biosystems, Cat# DS9800) with DAB as chromogen ...
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bioRxiv - Cell Biology 2020Quote: ... 4 µm thick sections of PC and DC were produced using a Leica CM3050 S Research Cryostat (Leica Microsystems) and mounted onto microscope slides ...
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bioRxiv - Developmental Biology 2022Quote: ... The sample was sectioned to a thickness of 4 µm using a Leica Autostainer XL (Leica Biosystems, Nussloch, Germany) and stained with hematoxylin and eosin (H&E) ...
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bioRxiv - Neuroscience 2020Quote: ... Mounted sections were stored at 4°C until they were imaged using a laser scanning confocal microscope (Leica SP8).