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Citations for Leica :
901 - 950 of 1061 citations for 2 Chloro 3 4 cyanobenzoyl pyridine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... Approximately 2 µL of the spore suspension per sample was then processed with Leica EM ICE HPF apparatus (Leica Microsystems), and then freeze substituted and embedded in Lowicryl HM20 (Electron Microscopy Sciences ...
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bioRxiv - Plant Biology 2022Quote: ... We cut the Spurr’s blocks into semi-thin sections (0.75–2 μm) with glass knives on an ultramicrotome Leica Ultracut UCT (Leica Microsystems) stained with a solution containing 1% (w/v ...
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bioRxiv - Cell Biology 2020Quote: ... Images were captured using Metamorph software at every 15 minutes in 37C (PeCon Tempcontrol 37-2) using confocal spinning disk system (Leica DMI 6000 B equipped with YOKOGAWA CSU-W1 Confocal Scanner Unit ...
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bioRxiv - Neuroscience 2021Quote: ... embedded in 2% LTE agarose/ Tris-buffered saline (TBS) and cut coronally in 50 µm sections on a vibratome (VT 1000S, Leica). Sections underwent antigen retrieval in a solution of 0.1 M sodium citrate (pH 6) ...
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bioRxiv - Immunology 2021Quote: ... Sections were serially imaged over the course of 1-2 hours and compiled using LAS X software (Leica Microsystems, USA). Image analysis was performed using Imaris software (Bitplane ...
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bioRxiv - Developmental Biology 2021Quote: ... The tissues were then cryoprotected in 10% and 30% sucrose solutions for 2 hrs at RT and finally incubated in 30% sucrose and tissue embedding medium (Leica) (1:1 ...
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bioRxiv - Developmental Biology 2020Quote: ... 25% laser power (Pnmy-2nmy-2::gfp) or 13% laser power (Ppie-1mCherry::phPLCδ) was used (Leica TCS SP5-II). All analyses were performed on center Z-slices of Z-stacks through the NSMnb ...
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bioRxiv - Neuroscience 2022Quote: ... 2-photon imaging was performed using an upright microscope optimized for confocal and multiphoton microscopy (TCS SP5 II, Leica Microsystems). After the mouse was mounted to a stereotaxic holder (SR-AM and STS-A ...
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bioRxiv - Cancer Biology 2022Quote: ... with antigen retrieval at 100ºC for 20 min with Epitope Retrieval Solution 1 or 2 respectively (AR9961, AR9640, Leica Biosystems). TMAs were blind-scored by multiplying staining intensity with staining coverage ...
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bioRxiv - Cancer Biology 2022Quote: ... Samples and probes were co-denatured at 75°C for 2 min followed by incubation at 37°C overnight in a humidified chamber using ThermoBrite System (Leica). Slides were then washed for 2 min in 0.4x SSC at 75°C followed by a 30 second wash in 2x SSC at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... the brain was cut into 2 mm thick coronal sections in 0.15 M cacodylate buffer using a vibratome (Leica VT1200). Then ...
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bioRxiv - Bioengineering 2022Quote: ... a ~ 2 nm layer of gold coating was completed using a sputtering machine (Leica EM ACE 600, Buffalo Grove, IL) and was imaged using an SEM (Apreo 2 ...
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bioRxiv - Systems Biology 2024Quote: ... Dried smears were incubated in 70% ethanol for 2 hours and hematoxylin/eosin (H&E) stained using an automatic Leica Multistainer and Coverslipper (Leica ST5020+CV5030 ...
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The transcriptomic landscape of monosomy X (45,X) during early human fetal and placental developmentbioRxiv - Genetics 2024Quote: ... IHC for CSF2RA (Colony Stimulating Factor 2 Receptor Subunit Alpha) was undertaken on 3µm sections using a Leica Bond-max automated platform (Leica Biosystems). In brief ...
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bioRxiv - Neuroscience 2023Quote: ... brains tissue fixed in 4% paraformaldehyde were cryoprotected in 30% sucrose for 2 hours followed by embedding in OCT for sectioning to 10μm thickness on a cryostat (Leica CM1860). Prior to staining ...
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bioRxiv - Immunology 2023Quote: ... as described previously.23 All heat induced epitope retrievals were performed with Bond™ Epitope Retrieval 2 (AR9640, Leica Biosystems) for 20 minutes at 95°C ...
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bioRxiv - Genetics 2023Quote: ... the worms were mounted on a 2% agarose pad and images were captured using a Leica compound microscope (Leica DM5000B).
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bioRxiv - Neuroscience 2023Quote: ... were imaged using a 0.75 NA 20X objective at 2 μm depth intervals on a Leica TCS SP8 laser scanning confocal microscope (Leica Microsystems). For the validation of the mouse line (assay # 1) ...
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bioRxiv - Cancer Biology 2023Quote: ... Non-consecutive sections were cut with a thickness of 10μm and placed onto 2 capture areas of 10X Visium Spatial Gene expression slide using a cryostat (Leica CM3050S). The tissue Optimization kit was used to determine permeabilization times (24 min) ...
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bioRxiv - Cell Biology 2022Quote: ... Embryos were injected intravascularly in the duct of Cuvier with 27,6 nL of Membright Cy5-labeled tEVs or 4T1CD63pHluorinmScarlet-tEVs (at 10E10 EVs/ml) with a Nanoject microinjector 2 (Drummond) under a M205 FA stereomicroscope (Leica) as described previously (Mary et al. ...
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bioRxiv - Plant Biology 2023Quote: ... leaving around 2 mm of the root outside the needle which was submerged in a tissue-freezing medium (Jung, Leica) and quickly frozen in liquid nitrogen (Vogel-Mikuš et al. ...
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bioRxiv - Neuroscience 2023Quote: ... five µm section were cut followed by a Heat-Induced Epitope Retrieval at 100°C during 20 min in BOND Epitope Retrieval 2 (ER2) solution (Leica) with 0.1 Proteinase K before Deparaffinizing ...
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bioRxiv - Neuroscience 2023Quote: ... the animals were placed under 0.8-1% isoflurane anesthesia (2% used for induction) and imaged on an upright laser-scanning confocal (Leica SP8) or upright laser-scanning two-photon microscope (Bruker Ultima ...
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bioRxiv - Neuroscience 2023Quote: ... The caudal corpus callosa alongside adjacent tissue were punched with a 2 mm diameter punching tool and embedded in Epon (EM TP, Leica) prior to conventional embedding steps ...
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bioRxiv - Developmental Biology 2023Quote: ... dehydrated in methanol and cleared in Benzyl Alcohol/Benzyl Benzoate (1/2) prior to mounting and imaging with a SP8X confocal microscope (Leica). A 3D binary mask was built using Fiji to obtain a binary image of the tissue ...
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bioRxiv - Developmental Biology 2024Quote: ... Slides were baked and dewaxed on Leica Bond-RX and pretreated with Bond Epitope Retrieval Solution 2 (ER2) (AR9640) from Leica at 100°C for 40 minutes ...
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bioRxiv - Developmental Biology 2024Quote: Immunofluorescent analysis and imaging were performed on a Leica SP5X Upright 2-Photon Confocal microscope using LAS AF software (Leica) and a Leica 63X (type ...
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bioRxiv - Neuroscience 2024Quote: Confocal acquisitions were performed using a Leica SP8 White Light Laser 2 on an inverted stand (Leica Microsystems, Mannheim, Germany) and with a x20 (NA 0.75 ...
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bioRxiv - Neuroscience 2024Quote: ... the hemisphere was gelatine-embedded and coronally sectioned between anteroposterior coordinates -2 mm and -4.8 mm (dorsal hippocampus) with a cryostat (Leica CM3050 S, Leica Biosystems). Sections of 40 µM were placed on anti-freeze solution [30% glycerol ...
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bioRxiv - Cell Biology 2024Quote: ... or 2 hours of 200nM BafA1 before immediate cryo-fixation by high pressure freezing (Leica EM ICE High Pressure Freezer), with 20% BSA in DMEM as a cryoprotectant ...
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bioRxiv - Neuroscience 2024Quote: ... They were then embedded in 2% agarose and sliced into coronal sections (70-100µm thick) using a Vibratome (Leica Microsystems). The slices were washed in 0.01M PBS ...
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bioRxiv - Neuroscience 2024Quote: ... frozen at -80°C for 30 minute then at -20°C for 2 hours before being sectioned using a cryostat (Leica, 50 µm thick coronal sections for immunocytochemistry) ...
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bioRxiv - Cell Biology 2024Quote: ... and EB3 comets in single cells were imaged for 60 seconds (1 frame per 2 seconds) with a Leica DMi8 microscope (Leica). Only cells exhibiting both tdTomato and EYFP signals were included in the analysis ...
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bioRxiv - Cell Biology 2024Quote: ... samples were freeze-substituted at -90°C in anhydrous acetone containing 2% osmium tetroxide and 0.1% uranyl acetate at -90°C for 72 hours and warmed to room temperature with a gradient of +5°C per hour (EM AFS-2, Leica). Sections of the samples were analysed in a Tecnai Spirit T12 Electron microscope equipped with an Eagle OCD camera (Thermo Fisher Scientific).
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bioRxiv - Evolutionary Biology 2024Quote: ... embryos were introduced into an M9 droplet on a 2% agarose pad and immediately imaged on a Leica SP8 confocal on a DM6000 upright microscope (Leica LAS X image acquisition software ...
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bioRxiv - Neuroscience 2021Quote: ... The brains were transferred and kept in 30% sucrose in PBS for more than 48 h at 4 °C and then sectioned using a freezing microtome (SM2010R, Leica; EF-13, Nihon Microtome Laboratory) at a thickness of 40 μm parallel to the coronal plane ...
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bioRxiv - Neuroscience 2020Quote: ... the rats were sacrificed via transcardiac perfusion with 4% paraformaldehyde (PFA) using the Perfusion Two™ Automated Pressure Perfusion System (Leica Biosystems, Buffalo Grove, IL) (n = 12 ...
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bioRxiv - Cancer Biology 2020Quote: ... was performed on 4-μm FFPE sections arranged on the above mentioned TMA using commercial equipment (Leica Bond RXm System; Leica Microsystems Ltd, Milton Keynes, UK) and a detection kit (Bond Polymer Refine Red Detection Kit ...
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bioRxiv - Neuroscience 2021Quote: The whole-mount samples were embedded in gelatin/albumin with 4% of Glutaraldehyde and sectioned at 20 μm thickness on a vibratome (Leica, VT1000 S vibrating blade microtome). The sections were mounted in Fluoromount Aqueous Mounting Medium (Sigma ...
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bioRxiv - Pathology 2022Quote: ... and incubated with a 1:200 dilution of anti-4-HNE and anti-AKR1A1 primary antibody overnight at 4°C primary antibody along with the Novolink™ Max Polymer Detection System (Leica Biosystems, Wetzlar, Hesse, Germany) and DAB substrate (3,30-diaminobenzidine ...
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bioRxiv - Neuroscience 2024Quote: ... Brains were post-fixed with PFA 4% overnight and 50 µm thick coronal sections were cut with a VT 1000S vibratome (Leica Microsystems Inc., Buffalo Grove, IL). Free-floating vibratome sections were processed as follows ...
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bioRxiv - Neuroscience 2023Quote: ... Brains were then fixed in 4% PFA overnight before sectioning 50 µm coronal slices using a Leica VT1000S vibratome (Leica Biosystems, Deer Park, IL, USA).
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bioRxiv - Neuroscience 2023Quote: ... Post-fixed brains (PFA 4%) were coronally sectioned at either 30 μm (IHC) with a cryostat (Leica CM 1950, Leica Biosystems, Deer Park, IL, USA). Mice left tibia was dissected and placed in 4% PFA then follow 2.10 Bone histology analysis method.
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bioRxiv - Biochemistry 2024Quote: ... The excess liquid was blotted for 4 s prior to plunge-freezing into liquid ethane using a Leica EM GP2 plunger (Leica Microsystems; 95% humidity, 14 °C). Grids were stored in liquid nitrogen prior to data collection ...
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bioRxiv - Cell Biology 2020Quote: ... Dried samples were then rotary-shadowed with 2 nm of platinum and 5-8 nm of carbon using an ACE600 high vacuum metal coater (Leica Microsystems). Platinum replicas were floated off the glass by 5% hydrofluoric acid ...
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bioRxiv - Cell Biology 2020Quote: ... cells were loaded with fura-2/AM in the dark (5 μM; 30 min; RT; S1) and imaged with an upright microscope (Leica DMI6000FS, Leica Microsystems) equipped for ratiometric live-cell imaging with a 150W xenon arc lamp ...
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bioRxiv - Cell Biology 2020Quote: ... The loosely packed cells in BSA were high-pressure frozen in a Leica Empact 2 high-pressure freezer (Leica, Vienna, Au), and submitted to a quick freeze-substitution protocol that preserves fluorescence (35 ...
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bioRxiv - Cell Biology 2022Quote: ... pre-blotted and blotted with filter paper (Whatman 1) for 2 sec followed by plunging into liquid ethane using a Leica EM GP (Leica Microsystems). The samples were stored in liquid nitrogen until data acquisition ...
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bioRxiv - Bioengineering 2021Quote: ... gravid day 1 adults animals were picked onto prepared palmitic acid plates and plates were imaged at 2 and 5 hours at 1.6x on a stereomicroscope (Leica M165 FC) using a 1.3 MP CMOS camera (Thorlabs DCC1645C ...
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bioRxiv - Cell Biology 2021Quote: ... and freeze substituted in anhydrous acetone containing 1 % OsO4/ 2% H2O for 64h using the Automatic Freeze Substitution unit (AFS; Leica Microsystems). The samples were included in Epon ...