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Citations for Leica :
851 - 900 of 996 citations for IL 19 Human since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2019Quote: ... The brain was snap frozen using 2-methylbutane and sliced into 40-50um coronal sections using a cryostat (Leica CM3050S, Leica Biosystems, Buffalo Grove, IL). Slices were mounted on gelatin covered microscope slides and allowed to dry ...
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bioRxiv - Microbiology 2019Quote: ... and placed under a Leica TCS SP5 confocal microscope for image acquisition using a Leica 63× oil immersion objective lens (Leica Microsystems, Buffalo Grove, IL).
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bioRxiv - Neuroscience 2019Quote: ... The brains were thawed to –16°C and sliced into ten series of 16 μm-thick coronal sections through the entire hypothalamus using a cryostat (CM3050 S, Leica Microsystems, Buffalo Grove, IL). Each section was thaw-mounted on Superfrost Plus slides (Fisher Scientific) ...
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bioRxiv - Neuroscience 2020Quote: ... Sagittal sections (25 μm thick) were cut using a Leica CM1900 cryostat at a chamber temperature of −20 °C (Leica Biosystems, Buffalo Grove, IL) and mounted onto Superfrost-Plus Gold microscope slides (Fisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... Brains were sectioned coronally in 30μm thick sections using a cryostat and mounted using a CryoJane tape transfer system (Leica Biosystems, Inc., Buffalo Grove, IL).
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bioRxiv - Biophysics 2020Quote: ... The image is obtained using ×25 or ×40 water immersion objective on a TCS-SP5 confocal laser scanning microscope (Leica Microsystems Inc., IL, USA).
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bioRxiv - Bioengineering 2021Quote: ... Brains were removed and horizontal cortical or hippocampal sections (300 μM thick) were cut in ice-cold ACSF using a VT1200S Vibratome (Leica, Buffalo Grove, IL, USA) and were recovered in ACSF for 30 minutes at 37 °C before treatment with MitoT-EVs.
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bioRxiv - Bioengineering 2020Quote: ... Samples were attached to SEM stubs using double-sided carbon tape and coated with Au/Pd for 30 seconds using an EM ACE200 sputtercoater (Leica, Buffalo Grove, IL, USA). Images were collected with a JSM-6010LA SEM (JEOL ...
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bioRxiv - Bioengineering 2020Quote: Networks were imaged with a DMi8 Yokogawa W1 spinning disk confocal microscope outfitted with a Hamamatsu EM-CCD digital camera (Leica Microsystems, Buffalo Grove, IL). Z-stacks were acquired with a depth of 200 µm and step size of 5 µm ...
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bioRxiv - Biochemistry 2020Quote: ... Frozen bovine lenses were sectioned to 10 μm thickness equatorially or axially using a CM 3050 Cryostat (LEICA CM 3050S, Leica Microsystems Inc., Bannockburn, IL). Sections were thaw-mounted on Indium-Tin-Oxide (ITO ...
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bioRxiv - Neuroscience 2021Quote: ... Z stacked images were acquired using the Axio Imager (Zeiss, Germany) upright fluorescence microscope and AxioVision Imaging System (Zeiss, Germany) software or a SP8 confocal microscope (Leica Microsystems, Buffalo Grove, IL). All images were processed using Imaris (Bitplane ...
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bioRxiv - Cancer Biology 2020Quote: ... At least three randomly-selected and non-overlapping fields on each core of the tissue microarray were examined using nuclear algorithm of the Aperio ImageScope software (Leica Biosystems, Buffalo Grove, IL). Data is expressed as H-score that is based on intensity (0 ...
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bioRxiv - Bioengineering 2021Quote: ... or Toluidine Blue Stain, 1% w/v (Ricca Chemical, Arlington, TX) and scanned with a Leica Aperio ScanScope® CS2 (Leica, Buffalo Brove, IL) system ...
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bioRxiv - Microbiology 2021Quote: ... Individual images were also obtained with a 100x objective on a DeltaVision II deconvolution microscope (Applied Precision Inc., Currently Leica Microsystems, Buffalo Grove, IL). Analysis was conducted using GraphPad Prism 9 with an ordinary one-way ANOVA multiple comparisons test and unpaired t-test.
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bioRxiv - Cancer Biology 2020Quote: ... Whole mount images of primary tumors and lungs were taken with a Leica MZ10 F modular stereo microscope (Leica Microsystems, Buffalo Grove, IL, USA).
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bioRxiv - Neuroscience 2020Quote: ... OCT-embedded brains were sectioned (35 µm) throughout on a horizontal (axial) plane with a cryostat (Leica CM3050S, Leica Biosystems, Buffalo Grove, IL, USA), and collected in individual wells ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were imaged using confocal microscopy (TCS-SPE with DM6 RGBV confocal microscope; Leica DFC7000 T camera; using Leica LAS X core software; Leica Microsystems; Buffalo Grove, IL) and a 20x objective ...
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bioRxiv - Neuroscience 2022Quote: ... were collected after sectioning with a vibratome (Technical Products International; St. Louis, MO) for visualization on a confocal microscope (Leica Biosystems, Buffalo Grove, IL). Alternately ...
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bioRxiv - Neuroscience 2022Quote: ... coronal brain slices (300 μm) containing BLA were prepared in the same sucrose-based aCSF solution using a vibratome (VT1200S; Leica Microsystems, Buffalo Grove, IL). Brain slices were kept in normal aCSF (in mM ...
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bioRxiv - Cancer Biology 2022Quote: ... Z-stack images were acquired with a step size of 0.9 µm with a Leica TCS SP5 confocal microscope (Leica Microsystems, Inc., Buffalo Grove, IL).
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bioRxiv - Neuroscience 2022Quote: All stained sections were digitally scanned using an Aperio scanner system (Aperio AT2 - High Volume, Digital whole slide scanning scanner, Leica Biosystems, Inc., Richmond, IL) and stored in Biolucida ...
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bioRxiv - Neuroscience 2022Quote: ... while the remaining sections were selected for immunohistochemistry procedures using a Leica Bond III automated immunostainer with a diaminobenzidine chromogen detection system (DS9800, Leica Biosystems, Buffalo Grove, IL). Immunohistochemistry was performed for glial fibrillary acidic protein (GFAP ...
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bioRxiv - Microbiology 2022Quote: ... cell supernatants were collected to quantify viral particle production using a plaque assay or the cytopathic effects were evaluated using an inverted microscope and an MCI70-HD camera (Leica, Buffalo Grove, IL, USA). For the virucidal assay ...
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bioRxiv - Bioengineering 2022Quote: ... Tube formation was visualized and brightfield images were recorded using a DMi8 Yokogawa W1 spinning disk confocal microscope outfitted with a Hamamatsu EM-CCD digital camera (Leica Microsystems, Buffalo Grove, IL) or Leica DMI4000 B (Leica ...
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bioRxiv - Plant Biology 2021Quote: ... The peel from abaxial side of the leaf was then placed on a microscope slide and images were collected using a DM6000B light microscope (Leica, Buffalo Grove, IL USA) This experiment was repeated 3 times to image 50 stomata from each replicated treatment and a total of 150 stomata measurements from 3 independent replicates were analyzed for each time point ...
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bioRxiv - Neuroscience 2021Quote: ... Mice brains were removed and dehydrated in 30% sucrose prior to flash-freezing with dry ice and slicing into 40 μm-thick coronal sections using a sliding microtome (Leica Biosystems, Buffalo Grove, IL). Slices were mounted and counterstained with DAPI (Vector Laboratories ...
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bioRxiv - Biochemistry 2021Quote: ... Slides were then dehydrated and cleared through graded alcohols and xylene and sealed with a coverslip and Micromount (Leica cat# 3801731, Buffalo Grove, IL) using a Leica CV5030 automatic coverslipper.
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bioRxiv - Cell Biology 2022Quote: ... Images were acquired with an ORCA-Flash 4.0 sCMOS camera (Hamamatsu Corp, Bridgewater, NJ) connected to a Leica DMi8 wide-field fluorescence microscope (Leica Microsystems, Inc., Buffalo Grove, IL). Image analysis was performed with ImageJ.
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bioRxiv - Cell Biology 2022Quote: ... time-series image acquisition was performed with an ORCA-Flash 4.0 sCMOS camera (Hamamatsu Corp, Bridgewater, NJ) connected to a Leica DMi8 wide-field fluorescence microscope (Leica Microsystems, Inc., Buffalo Grove, IL).
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bioRxiv - Bioengineering 2020Quote: U87-MG migration across the microfluidic device was monitored daily by acquiring fluorescent images of the central gel channel with a DMi8 Yokogawa W1 spinning disk confocal microscope with a Hamamatsu EM-CCD digital camera (Leica Microsystems, Buffalo Grove, IL). Three regions of interest (ROI ...
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bioRxiv - Cell Biology 2019Quote: ... Sample block was then re-mounted using Durcupan on a copper stud (Xu et al., 2017) and trimmed using ultramicrotome (EM UC7, Leica Microsystems, Buffalo Grove, IL). Finally ...
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bioRxiv - Pathology 2021Quote: Four micron thick sections of left and right ventricular FFPE tissue blocks per decedent were cut and labeled using an automated staining platform (Leica Bond 3, Leica Biosystems, Buffalo Grove, IL) with the following monoclonal antibodies against ...
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bioRxiv - Neuroscience 2021Quote: ... Brains were rinsed 3 times in phosphate buffered saline and 50 μM coronal brain slices were obtained using a vibratome (Leica VT1000, Buffalo Grove, Il), without embedding the tissue ...
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bioRxiv - Neuroscience 2021Quote: ... immersed in ice-cold 95% O2/5% CO2-equilibrated sACSF and sectioned at 250 µm in the sagittal plane with a vibratome (VT1200S; Leica Microsystems Inc., IL, USA). Slices were then transferred to a holding chamber ...
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bioRxiv - Neuroscience 2020Quote: ... submerged in the cutting solution and 250μm sagittal slices from the vermis were obtain using a Leica VT1200S vibratome (Leica Biosystems Inc. Buffalo Grove, IL). Slices were transferred into an incubation chamber with artificial cerebral spinal fluid (ACSF ...
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bioRxiv - Neuroscience 2021Quote: ... 8–10 week old mice received a single impact onto the skull at bregma using an ImpactOne stereotaxic impactor (Leica Biosystems, Buffalo Grove, IL) with a 3-mm-diameter tip (velocity set at 4.0 m/s ...
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bioRxiv - Cancer Biology 2020Quote: Five micron sections of tumors were immunolabeled with one of the following antibodies using a Leica Bond III automated immunohistochemistry instrument (Leica Biosystems, Buffalo Grove, IL): Rabbit anti-CD3 ...
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bioRxiv - Cancer Biology 2021Quote: ... IHC staining for CAV1 was performed on formalin-fixed paraffin-embedded HER2-expressing gastric tumor sections (5 μm thickness) on a Leica Bond RX automated stainer (Leica Biosystems, Buffalo Grove, IL). Following deparaffinization and heat-induced epitope retrieval in a citrate buffer at pH 6.0 ...
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bioRxiv - Neuroscience 2021Quote: ... and sacrificed via transcardiac perfusion with 10% sucrose (isotonic at 9.25% in distilled water; prewash) and 4% paraformaldehyde (PFA; fixative) using the Perfusion Two™ Automated Pressure Perfusion System (Leica Biosystems, Buffalo Grove, IL) (n = 12 ...
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bioRxiv - Neuroscience 2022Quote: ... and images of the sections were taken at 10x and 20x magnification using Leica DMI3000 B manual inverted fluorescence microscope (Leica Microsystems, Buffalo Grove, IL).
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bioRxiv - Neuroscience 2022Quote: ... The cryoprotected blocks were then flash-frozen using dry ice and sectioned at 40 μm using a Leica SM2000R sliding microtome (Leica Biosystems, Deer Park, IL). For immunohistochemistry ...
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bioRxiv - Neuroscience 2023Quote: ... the brains were quickly removed from the skull and cut into 200 µm thick coronal slices using a Leica vibratome VT 1200S (Leica Biosystems, Buffalo, IL, USA). The slices were then incubated for 30 minutes at 34 °C in the isolation NMDG buffer and for another 30 minutes in artificial cerebrospinal fluid (aCSF ...
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bioRxiv - Neuroscience 2022Quote: ... embedded them in 4% w/v agar/PBS and prepared 40 µm thick slices using a vibrating blade microtome (VT1200S, Leica Microsystems, Buffalo Grove, IL). The slices were post-fixed in 4% PFA/PBS for 30 min at room temperature (RT ...
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bioRxiv - Neuroscience 2022Quote: ... 0.4 mM L-ascorbic acid and the pH was adjusted to 7.4) and then sliced on a vibratome (Leica, VT1200 S, Deerfield, IL, USA). Brain slices (300 μm thick ...
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bioRxiv - Cancer Biology 2022Quote: Tissue sections were incubated for 1 hour in primary antibodies and detected using the BOND Polymer Refine Detection System (DS9800; Leica Biosystems, Buffalo Grove, IL) in accordance with the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... The brains were embedded in 3% agarose gel in a cubic cryomold and coronally sectioned at 50 μm thickness using a VT1000S vibrating blade microtome (Leica Biosystems, Buffalo Grove, IL). The sections were rinsed in PBS ON at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... The brass planchets were carefully removed from the polymerized resin and semi-thin (300 nm) sections were cut on a Leica Reichart Ultracut (Leica Microsystems, Buffalo Grove, IL), stained with 0.5% Toluidine Blue in 1% aqueous sodium borate (Fisher ...
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bioRxiv - Cell Biology 2022Quote: ... The ratio of bacterial particles per macrophage was quantified by taking photographs of three fields for each well using a Leica DM4000 B microscope (Leica Microsystems, Buffalo Grove, IL) with a 20x objective.
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bioRxiv - Neuroscience 2022Quote: ... mice were anaesthetized using 2.5% isoflurane and 1.5 l/min air for 5 min and impacted directly over their intact head using an electromagnetic impactor (Leica Impact One Stereotaxic impactor, Leica Biosystems, Richmond, IL, USA) with a 3mm round tip at a speed of 3m/s ...
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bioRxiv - Cancer Biology 2023Quote: ... NAT was chosen from each of 11 tumors with available slides and subjected to whole slide scanning at 400× magnification using an Aperio XT scanner (Leica Biopsystems, Buffalo Grove, IL). Images were visualized using ImageScope software (Leica Biosystems) ...