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Citations for Leica :
851 - 900 of 1274 citations for Acetaldehyde 3a 4 5 6 7 7a hexahydro 4 7 methano 1H inden 5 yl oxy since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... 300-320 mOsmol/kg) saturated with a mix of 95% O2 and 5% CO2 using a VT1000S vibratome (Leica). Slices were transferred to a submerged chamber and continuously perfused with artificial CSF (in mM ...
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bioRxiv - Neuroscience 2024Quote: ... The lumbar spinal cord (L1-5) was sectioned transversely (300µm) in dissection solution using a vibrating microtome (Leica Microsystems). Slices were immediately transferred to recording artificial cerebrospinal fluid (ACSF ...
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bioRxiv - Neuroscience 2024Quote: ... 1.2 NaH2PO4 and 0.5 CaCl2 bubbled with 95% O2/5% CO2 (pH of 7.4) using a vibratome (VT1200S, Leica Biosystems). Coronal slices (280 µm thick ...
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bioRxiv - Neuroscience 2024Quote: ... 20 mM choline bicarbonate) saturated with carbogen (5% CO2 and 95% O2) using a vibratome (Leica VT 1200 S). 250 μm brain slices were incubated in 31°C ACSF solution (119 mM NaCl ...
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bioRxiv - Neuroscience 2024Quote: ... Lumbar spinal cords (L3-5) were harvested and transversely sectioned using a LEICA CM3050 S Cryostat (LEICA, Wetzlar, Germany). For immunostaining ...
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bioRxiv - Neuroscience 2024Quote: ... The bulbs were embedded in 5 % agarose and cut coronally into 50 µm slices using a vibratome (VT1000S Leica).
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bioRxiv - Bioengineering 2024Quote: ... Images (n = 5) of S-shape embedded filaments were taken with a confocal microscope (SPX8, Leica Microsystems, The Netherlands) and analyzed with ImageJ.
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bioRxiv - Cell Biology 2024Quote: ... Image acquisition of fixed embryo and egg chamber samples was performed on an inverted Stellaris 5 confocal microscope (Leica) with a 63x/1.4 NA oil immersion objective ...
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bioRxiv - Neuroscience 2024Quote: ... and 6 m/s velocity (Leica MyNeuroLab Impact One Stereotaxic Impactor ...
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bioRxiv - Immunology 2021Quote: ... Nucleocapsid Antibody (SINO BIO, 40143-R019) through LEICA BOND RX 1h RT and developed with Bond Polymer Refine Detection (Leica, DS9800). Brightfield images were acquired through an Aperio Scanscope System CS2 microscope and an ImageScope program (Leica Biosystem ...
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bioRxiv - Immunology 2021Quote: ... Nucleocapsid Antibody (SINO BIO, 40143-R019) through LEICA BOND RX 1h RT and developed with Bond Polymer Refine Detection (Leica, DS9800). Brightfield images were acquired through an Aperio Scanscope System CS2 microscope and an ImageScope program (Leica Biosystem ...
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bioRxiv - Neuroscience 2023Quote: ... Tissue blocks were warmed to −20°C for at least 1h prior to sectioning and sectioned at 18 µm using a cryostat (Leica CM3050S) at −20°C ...
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bioRxiv - Cell Biology 2020Quote: ... Tissues were embedded in 4% low temperature gelling agarose and cut into 100-150 micrometer thick section using a vibratome (Leica VT1200 S). The Proximity Ligation Assay (PLA ...
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bioRxiv - Cancer Biology 2021Quote: Larvae fixed in 4% PFA overnight at 4°C were embedded in 4% low melting temperature agarose and transverse sections collected at 200 µm intervals using a vibrating microtome (Leica VT 1000S). Sections were blocked with 1% BSA in PBS/0.3% Triton X-100 and incubated with a 1:750 dilution of mAb414 (Abcam ...
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bioRxiv - Neuroscience 2020Quote: ... brains were incubated in 10% and then 30% sucrose in PBS for 12-24 hours at 4 °C before being cut into 50 µm sections by a freezing-sliding microtome (Leica SM 2101R). Sections from V1 were mounted on gelatin-coated slides and cover-slipped with Prolong Diamond Antifade Mounting Media (P36965 ...
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bioRxiv - Neuroscience 2020Quote: ... brains were post-fixed for 24 hours and then included in agar 4% and serial cuts of 40 µm thick were made using vibratome (Leica Biosystems, S2000).
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bioRxiv - Cancer Biology 2021Quote: ... The specimens were embedded in paraffin blocks and serial sections of 4 μm thickness were cut using a microtome (Leica Biosystems, Germany). The sections were stained by Hematoxylin & Eosin (H&E)34 Histological photographs were obtained using a digital light microscope (Olympus ...
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bioRxiv - Neuroscience 2020Quote: ... Brains were postfixed in 4 % PFA for 24 hours until they were sliced (70 µm thickness) using a Leica Vibratome (Leica, Wetzlar, Germany). Slices were directly mounted on an object slide (Gerhard Menzel ...
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bioRxiv - Neuroscience 2020Quote: ... brains were incubated in 10% and then 30% sucrose in PBS for 12-24 hours at 4 °C before being cut into 50 µm sections by a freezing-sliding microtome (Leica SM 2101R). Sections with LGN and V1 were then mounted on gelatin-coated slides and cover-slipped with mounting media (Prolong Diamond Antifade Mounting Media ...
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bioRxiv - Microbiology 2022Quote: ... Grids were sputter-coated with a ∼4 nm thick layer of tungsten using ACE600 cryo-sputter coater (Leica Microsystems GmbH, Vienna, Austria) and afterwards transferred into a Crossbeam 550 FIB-SEM dual-beam instrument (Carl Zeiss Microscopy ...
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bioRxiv - Cancer Biology 2022Quote: ... and incubated overnight at 4°C in 0.05% Digitonin-Antibody buffer containing a rabbit anti mouse-p53 Ab (1:100 Leica, NCL-p53-CM5p). Samples incubated with rabbit IgG (1:150 ...
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bioRxiv - Neuroscience 2021Quote: ... The brains were post-fixed overnight in 4% PFA and stored in PBS until histological slicing was performed using a vibratome (Leica VT1200 S) and the slices were mounted in DAPI-Fluoromount-G (70-100 µm slices ...
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bioRxiv - Cancer Biology 2021Quote: ... and red tdTomato/CMDiI channels and were recorded as approximately 1 x 4 stitches at 10x magnification using a Leica sp8 confocal microscope (Leica, Wetzlar, Germany). Consecutive stitch sequences were processed into a single image using Fiji 33 using the plugin by Preibisch et al. ...
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bioRxiv - Bioengineering 2020Quote: ... eyes were fixed in 4% paraformaldehyde (w/v) in PBS for 1 hour and embedded in optimal cutting temperature compound (Leica Biosystems, Germany) and stored at -80C until cryosectioning ...
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bioRxiv - Developmental Biology 2022Quote: ... Electroporated hemispheres were infiltrated with 30% sucrose at 4°C overnight prior to embedding in TissueTek tissue freezing medium (Leica Biosystems, Germany). 16 µm-thick sections were cut using a cryostat (Leica Biosystems ...
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Genome modularization reveals overlapped gene topology is necessary for efficient viral reproductionbioRxiv - Synthetic Biology 2020Quote: ... blotting for 3-seconds at 4 °C with 90% humidity and plunging into liquid ethane using a Lecia EM GP device (Leica Microsystems, Germany). Cryo-EM data were collected on a Talos Arctica transmission electron microscope operated at 200 kV acceleration voltage using EPU automated data collection software (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... Formalin-fixed paraffin-embedded (FFPE) samples were cut to a thickness of 4 μm and stained on an automated stainer (BOND-MAX, Leica Biosystems, Germany) as part of the diagnostic routine in a clinically accredited histology lab ...
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bioRxiv - Immunology 2020Quote: ... at which time tissues were removed from the BSL-3 laboratory and stored in 1X PBS at 4°C until processed in a Leica PELORIS automated vacuum infiltration processor (Leica, Wetzlar, Germany), followed by paraffin embedding with a Histocore Arcadia paraffin embedding machine (Leica) ...
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bioRxiv - Cell Biology 2021Quote: ... Osmicated samples were dehydrated in graded ethanols (25% to 100%) at 4°C and stored in 100% acetone until critical point drying with liquid CO₂ (EM CPD300, Leica Microsystems Ltd.). Samples were mounted onto stubs using silver paint (Agar Scientific) ...
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bioRxiv - Neuroscience 2023Quote: ... Ribbons of 150-200 serial sections of thicknesses of approximately 55nm were cut with 4 drops of pure ethanol in the water boat and an ionizing instrument (Leica EM Crion) activated and oriented towards the cutting edge of the knife from above ...
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bioRxiv - Neuroscience 2023Quote: ... Brains not used for two-photon imaging were postfixed overnight in 4% paraformaldehyde in PBS on a shaker at 4°C and cut into 50 µm coronal (S1 injections) or parasagittal (SNc injections) sections on a vibrating microtome (Leica, VT-1000S). Sections were collected sequentially into 6 tubes containing cryoprotectant ...
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bioRxiv - Neuroscience 2023Quote: ... the samples were dehydrated using an ascending ethanol series (30%-100%) at 4℃ and then critically dried using a low-speed critical point dryer (Leica EM CPD300). Subsequently ...
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bioRxiv - Neuroscience 2023Quote: ... Brains were postfixed overnight in 4% paraformaldehyde in PBS on a shaker at 4°C and cut into 50 μm coronal sections on a vibrating microtome (Leica, VT-1000S). Sections were collected sequentially into 6 tubes containing cryoprotectant ...
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bioRxiv - Developmental Biology 2023Quote: ... The oocytes were fixed in 4% PFA/PBS for 30 min and observed using the Leica M205FA stereomicroscope (Leica Microsystems, Wetzlar, Germany) or FV3000 Confocal Microscope.
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bioRxiv - Cell Biology 2023Quote: ... 30% sucrose in PBS (2:1 v/v) over-night (O/N) at 4°C with gentle shaking and frozen in OCT (Leica Surgipath, FSC22), using plastic molds (Leica Surgipath ...
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bioRxiv - Bioengineering 2024Quote: ... The fixed constructs were embedded in low-melting point agarose 4% w/v in pre-warmed PBS and sliced to a thickness of 300 μm with a vibratome (Leica Biosystems, VT1000S) filled with PBS 1x ...
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bioRxiv - Bioengineering 2024Quote: ... and fetal pig kidneys were fixed with 4% paraformaldehyde (FUJIFILM Wako Pure Chemical Corporation) and sectioned with a cryostat (Leica Microsystems GmbH) to prepare frozen sections ...
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bioRxiv - Cell Biology 2023Quote: ... organoids were cryoprotected in 30% sucrose/PBS on a rotor shaker overnight at 4°C and sectioned at 20 µm on a cryostat (Leica 1850 UV). Sections were permeabilized in 0,3% Triton X-100/PBS ...
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bioRxiv - Neuroscience 2024Quote: ... the brains were embedded in 4% oxidized agarose as previously described (Ragan et al., 2012) to limit artifacts during sectioning on a vibratome (Leica VT1000 S). For 20 μm cryotome sectioning ...
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bioRxiv - Immunology 2024Quote: ... The brains were quickly dissected and fixed in 4% paraformaldehyde (PFA) overnight and then cut into 30 µm sections using a vibrating microtome (Leica, VTS1200, Germany). For immunostaining ...
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bioRxiv - Immunology 2024Quote: ... and tonsil sections of 4 µm-thick were deparaffinized at 70°C in 100% Clearene solvent (Leica Biosystems, Buffalo Grove, IL, USA) solution and washed in ethanol (EtOH ...
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bioRxiv - Neuroscience 2024Quote: Hemibrains were drop-fixed in 4% paraformaldehyde for two days and sectioned at 40 mm on a vibratome (Leica, Buffalo Grove, IL). Sections were collected in a 1 in 4 series and stored in PBS at 4°C ...
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bioRxiv - Immunology 2024Quote: ... Brains were placed in 4 °C s-ACSF and sectioned with a vibrotome at 350–400 μm thick (Leica VT1200S; Leica, Nusslock, Germany). Collected slices were then incubated at 32 °C for 1 h in i-ACSF (124 mM/L NaCl ...
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bioRxiv - Microbiology 2024Quote: ... sucrose in PBS overnight at 4°C and embedded in OCT compound (Agar Scientific) and cryosectioned (10 µm thick, Leica CM1850 Cryostat). Sections were dried overnight onto Thermo Scientific SuperFrost Plus Adhesion slides ...
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bioRxiv - Developmental Biology 2024Quote: ... The tissues were then embedded in paraffin wax and cut into 4-μm sections with a microtome (RM 2165; Leica Microsystems GmbH). Sections were stained with haematoxylin and hemoglobin (H&E) ...
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bioRxiv - Molecular Biology 2021Quote: ... embedded in OCT tissue freezing medium and 5 μm cross-sectioned on a Cryostat (Leica CM3050 S, Leica Biosystems, USA). Cross-sections were placed in 1x TBS for 10 minutes ...
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bioRxiv - Molecular Biology 2021Quote: ... embedded in OCT tissue freezing medium and 5 μm cross-sectioned on a Cryostat (Leica CM3050 S, Leica Biosystems, USA). Cross-sections were placed in 1x TBS for 10 minutes ...
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bioRxiv - Bioengineering 2020Quote: ... Sections were placed on the ice block to dry for 5 minutes prior to LCM using a laser microdissection microscope (Leica).
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bioRxiv - Developmental Biology 2021Quote: ... Stained embryos were embedded in Technovit 7100 resin (Kulzer Technique) and 5 µm sections were obtained using an RM2245 microtome (Leica). Sections were counterstained with Mayer’s hematoxylin solution and images were captured by BX61 microscope (Olympus ...
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bioRxiv - Biophysics 2020Quote: ... These polyps were then embedded in 5 % Agar and sectioned into 200 μm slices using a vibratome (Leica VT1200 S). The sections were then stained with 1 % Osmium tetroxide (Sigma) ...