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Citations for Leica :
851 - 900 of 1509 citations for 7 Chloro 1 2 3 4 tetrahydroisoquinoline since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... After the brain samples were fixed in 4% paraformaldehyde overnight, they were dehydrated in 30% sucrose (Daejung, Korea) and embedded in frozen section compound (Leica, Germany). Coronal sections (10 µm ...
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bioRxiv - Neuroscience 2023Quote: ... Brains were removed and postfixed overnight at 4°C and then cut into 50 µm coronal sections using a vibratome (#TPI1000; Ted Pella, or VT1000P; Leica). Sections were collected in cryoprotectant (25% glycerol ...
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bioRxiv - Neuroscience 2024Quote: ... Brains were post-fixed overnight at 4°C and cut into 30 μm-thick free-floating coronal sections with a vibrating microtome (Leica, VT1000). All the following steps were done with gentle shaking ...
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bioRxiv - Microbiology 2024Quote: ... Grids were sputter-coated with a ∼4 nm thick layer of tungsten using an ACE600 cryo-sputter coater (Leica Microsystems GmbH) and afterwards transferred into a Crossbeam 550 FIB-SEM dual-beam instrument (Carl Zeiss Microscopy) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The paraffin blocks were polymerized at 4°C and cut into 5-µm thick sections using a microtome (RM2155, Leica, Germany). The sections were mounted on microscope slides coated with egg white-glycerin and stained using Delafield’s Hematoxylin and Eosin (HE ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... The other hemibrain was drop-fixed in 4% paraformaldehyde in phosphate-buffered saline (PBS) and sectioned (30 μm) with a sliding microtome (Leica SM2000R). All mouse experiments were performed in accordance with European Union Council Directive 86/609/EEC ...
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bioRxiv - Neuroscience 2024Quote: ... Brains were stored in 4% formaldehyde for 24h at 4°C after which they were transferred to 30% sucrose solution at 4°C until they were cut in 50 μm thick coronal slices with a cryostat (Leica, Germany). The brain slices were washed twice for 10 minutes with phosphate buffered saline (PBS) ...
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bioRxiv - Cell Biology 2020Quote: Sample blocks were obtained by freeze substitution of the cell pellets in 0.5 % uranyl acetate diluted from a 20 % stock in methanol and 3 % double distilled H2O in acetone in a Leica AFS1 (Leica Microsystems GmbH, Wetzlar, Germany). Temperature was set to −130 °C for the first 2 hours and then raised to −90 °C with a 20 °C/hr gradient ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Samples were imaged in brightfield using a Point Grey Flea®3 color camera (Teledyne FLIR, Wilsonville, OR) mounted on a Leica S8 APO stereo microscope (Leica Camera, Wetzlar, Germany). Representative images are shown.
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bioRxiv - Pathology 2021Quote: Four micron thick sections of left and right ventricular FFPE tissue blocks per decedent were cut and labeled using an automated staining platform (Leica Bond 3, Leica Biosystems, Buffalo Grove, IL) with the following monoclonal antibodies against ...
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bioRxiv - Neuroscience 2021Quote: ... Brains were rinsed 3 times in phosphate buffered saline and 50 μM coronal brain slices were obtained using a vibratome (Leica VT1000, Buffalo Grove, Il), without embedding the tissue ...
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bioRxiv - Bioengineering 2022Quote: ... Samples were washed an additional 3 times in 100% ethanol for 15 minutes each and dried using a Leica EM CPD030 critical point dryer (Leica Microsystems, Buffalo Grove, IL) with CO2 as the transitional medium ...
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bioRxiv - Bioengineering 2023Quote: ... were acquired over a ∼30 mm x ∼3 mm rectangular area by using the tile scan feature of the LAS X software (Leica Microsystems, Buffalo Grove, IL). This involved frame by frame acquisition (36 × 5 = 180 frames ...
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bioRxiv - Cell Biology 2020Quote: ... The frozen samples were subsequently transferred into a Leica EM AFS-2 freeze substitution unit (Leica Microsystems). Over a period of five days ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 0.05% uranyl acetate in acetone and placed into an AFS-2 Freeze Substitution Machine (Leica Microsystems, Austria). Samples were freeze-substituted at −90°C for 72 h ...
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bioRxiv - Physiology 2021Quote: ... CFP and DsRed fluorescence signal were detected after 2 days with a CLSM (Leica AF6000 modular microsystems). All experiments were biologically repeated for three times.
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bioRxiv - Neuroscience 2021Quote: ... or inferior colliculus were cut at 300 µm with a vibratome (VT1200S, Leica, or 7000smz-2, Campden) in warm aCSF ...
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bioRxiv - Plant Biology 2021Quote: ... the embedded samples were sectioned at 2–5 μm thickness using a rotary microtome (Leica, Wetzlar, Germany) and stained with basic fuchsin ...
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bioRxiv - Neuroscience 2020Quote: ... Antigen retrieval was done by incubation with Bond Epitope Retrieval solution 2 (AR9640, Leica Biosystems, Wetzler, Germany) for 20 minutes and then sections were then washed with Bond Wash solution 3x ...
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bioRxiv - Cell Biology 2020Quote: ... 0.5 % glutaraldehyde and 0.25 % uranyl acetate for 60 h in a FS system (AFS-2, Leica Microsystems). Larvae were embedded in an Epon-Araldite mix (hard formula ...
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bioRxiv - Cancer Biology 2019Quote: ... Slides for CD14 and CD163 stains were retrieved for 20 minutes using Epitope Retrieval 2 (EDTA; Leica) incubated in protein block (Rodent Block M ...
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bioRxiv - Bioengineering 2022Quote: ... and counted for expression of opie-2-dsRed and 3xP3-tdTomato using a fluorescent stereoscope (Leica M165FC). The difference in total larval counts compared to total egg counts were considered to be dead during embryonic or early larval stages ...
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bioRxiv - Cell Biology 2022Quote: ... 0.05% uranyl acetate in acetone and placed in an AFS-2 Freeze-Substitution Machine (Leica Microsystems, Vienna). Samples were freeze-substituted at -90°C for 72 h ...
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bioRxiv - Developmental Biology 2022Quote: ... 0.5% glutaraldehyde and 0.25% uranyl acetate for 60 h in an FS system (AFS-2; Leica Microsystems). The embryos were embedded in an Epon-Araldite mix (EMS hard formula) ...
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bioRxiv - Immunology 2022Quote: ... Paraffin embedded kidney sections (2 µm) were cut on a Leica RM 2165 microtome (Leica, Wetzlar, Germany) and dried at 60°C for 1 h ...
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bioRxiv - Cell Biology 2023Quote: ... images were captured every 2 h for 72 h by the IncuCyte Live-Cell analysis system (Leica).
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bioRxiv - Developmental Biology 2023Quote: ... 0.5% glutaraldehyde and 0.25% uranyl acetate for 60 h in a FS system (AFS-2, Leica Microsystems). Larvae were embedded in Epon-Araldite mix (EMS hard formula) ...
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Acinar-to-ductal metaplasia in the pancreas requires a glycolytic switch and functional mitochondriabioRxiv - Cancer Biology 2022Quote: ... 2 μm thin slides were then prepared and immunohistochemistry was performed on a Bond Rxm system (Leica) with primary antibodies against Hexokinase 1 (1:500 ...
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bioRxiv - Plant Biology 2023Quote: ... Semi thin (2 μm) and ultra-thin (70 nm) sections were cut with an ultra-microtome (Leica Ultracut ...
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bioRxiv - Cancer Biology 2023Quote: ... Antigen was unmasked with Tris-EDTA pH 9 (Bond Epitope Retrieval Solution 2, Leica Biosystems, cat# AR9640).
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bioRxiv - Developmental Biology 2021Quote: ... 1/1000 (Leica biosystems), Rabbit anti-PH3 ...
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bioRxiv - Cell Biology 2019Quote: ... submersed in the media chamber containing 4°C Phosphate buffered Saline and cut using a Leica VT1200S vibrating blade microtome (Leica Biosystems, UK) at a speed 0.3mm/sec ...
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bioRxiv - Cell Biology 2020Quote: ... Tissues were embedded in 4% low temperature gelling agarose and cut into 100-150 micrometer thick section using a vibratome (Leica VT1200 S). The Proximity Ligation Assay (PLA ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The paraffin blocks were polymerized at 4°C and cut into 5 µm thick sections using a microtome (RM2155: Leica, Wetzlar, Germany). The sections were mounted on microscope slides coated with egg white-glycerin and stained using Delafield’s Hematoxylin and Eosin staining ...
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bioRxiv - Cancer Biology 2021Quote: Larvae fixed in 4% PFA overnight at 4°C were embedded in 4% low melting temperature agarose and transverse sections collected at 200 µm intervals using a vibrating microtome (Leica VT 1000S). Sections were blocked with 1% BSA in PBS/0.3% Triton X-100 and incubated with a 1:750 dilution of mAb414 (Abcam ...
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bioRxiv - Neuroscience 2020Quote: ... brains were incubated in 10% and then 30% sucrose in PBS for 12-24 hours at 4 °C before being cut into 50 µm sections by a freezing-sliding microtome (Leica SM 2101R). Sections from V1 were mounted on gelatin-coated slides and cover-slipped with Prolong Diamond Antifade Mounting Media (P36965 ...
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bioRxiv - Neuroscience 2020Quote: ... brains were post-fixed for 24 hours and then included in agar 4% and serial cuts of 40 µm thick were made using vibratome (Leica Biosystems, S2000).
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bioRxiv - Cancer Biology 2021Quote: ... The specimens were embedded in paraffin blocks and serial sections of 4 μm thickness were cut using a microtome (Leica Biosystems, Germany). The sections were stained by Hematoxylin & Eosin (H&E)34 Histological photographs were obtained using a digital light microscope (Olympus ...
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bioRxiv - Neuroscience 2020Quote: ... Brains were postfixed in 4 % PFA for 24 hours until they were sliced (70 µm thickness) using a Leica Vibratome (Leica, Wetzlar, Germany). Slices were directly mounted on an object slide (Gerhard Menzel ...
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bioRxiv - Neuroscience 2019Quote: ... Brains were post-fixed overnight in 4% paraformaldehyde and then cryoprotected in a 30% sucrose and 0.1M PBS solution prior to sectioning (Leica CM3050S, Leica Biosystems). Three sets of immediately adjacent sections (40 μm ...
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bioRxiv - Neuroscience 2020Quote: ... brains were incubated in 10% and then 30% sucrose in PBS for 12-24 hours at 4 °C before being cut into 50 µm sections by a freezing-sliding microtome (Leica SM 2101R). Sections with LGN and V1 were then mounted on gelatin-coated slides and cover-slipped with mounting media (Prolong Diamond Antifade Mounting Media ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The paraffin blocks were polymerized at 4°C and cut into 5 µm thick sections using a microtome (RM2155: Leica, Wetzlar, Germany). The sections were mounted on microscope slides coated with egg white-glycerin and stained using Delafield’s Hematoxylin and Eosin staining ...
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bioRxiv - Molecular Biology 2022Quote: ... Green siliques were harvested 12 d after flowering from 4-6 week old plants and opened using fine needles under a dissecting microscope (Leica MZ9.5, Germany). Images of opened siliques were captured using a Leica DFC290 (Germany ...
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bioRxiv - Microbiology 2022Quote: ... Grids were sputter-coated with a ∼4 nm thick layer of tungsten using ACE600 cryo-sputter coater (Leica Microsystems GmbH, Vienna, Austria) and afterwards transferred into a Crossbeam 550 FIB-SEM dual-beam instrument (Carl Zeiss Microscopy ...
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bioRxiv - Neuroscience 2021Quote: ... The brains were post-fixed overnight in 4% PFA and stored in PBS until histological slicing was performed using a vibratome (Leica VT1200 S) and the slices were mounted in DAPI-Fluoromount-G (70-100 µm slices ...
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bioRxiv - Developmental Biology 2022Quote: ... Electroporated hemispheres were infiltrated with 30% sucrose at 4°C overnight prior to embedding in TissueTek tissue freezing medium (Leica Biosystems, Germany). 16 µm-thick sections were cut using a cryostat (Leica Biosystems ...
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bioRxiv - Plant Biology 2019Quote: ... A series of 4-μm thick longitudinal sections and transverse sections (rapidly elongating region) were made with a rotary microtome RM2265 (LEICA EM UC7). Fixed sections were stained with toluidine blue O for 30 min and photographed with an Olympus BX51 microscope equipped with a DP74 camera.
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bioRxiv - Neuroscience 2019Quote: ... Brains were postfixed in 4% paraformaldehyde overnight on a shaker at 4°C and cut into 50 μm coronal sections on a vibrating microtome (Leica, VT-1000S), with sections collected into six tubes (containing cryoprotectant solution as described[32] ...
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bioRxiv - Cancer Biology 2021Quote: ... Formalin-fixed paraffin-embedded (FFPE) samples were cut to a thickness of 4 μm and stained on an automated stainer (BOND-MAX, Leica Biosystems, Germany) as part of the diagnostic routine in a clinically accredited histology lab ...
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bioRxiv - Immunology 2020Quote: ... at which time tissues were removed from the BSL-3 laboratory and stored in 1X PBS at 4°C until processed in a Leica PELORIS automated vacuum infiltration processor (Leica, Wetzlar, Germany), followed by paraffin embedding with a Histocore Arcadia paraffin embedding machine (Leica) ...