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Citations for Leica :
851 - 900 of 1036 citations for 4 Methoxy 6 methyl 6 phenyl 5H pyran 2 one since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... 0.4 mM L-ascorbic acid and the pH was adjusted to 7.4) and then sliced on a vibratome (Leica, VT1200 S, Deerfield, IL, USA). Brain slices (300 μm thick ...
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bioRxiv - Developmental Biology 2024Quote: ... Sections were mounted with Fluorsave (Table 1S) and stored in the dark at 4°C until imaging on a Leica Stellaris5 confocal microscope (Leica Microsystems CMS GmbH, Germany). Quantification was performed as previously described42 ...
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bioRxiv - Biochemistry 2023Quote: ... the grids were flash-frozen in liquid ethane at ∼90 % humidity and 4 °C with a Leica EM GP rapid-plunging device (Leica, Buffalo Grove, IL, USA) before being transferred into liquid nitrogen for storage ...
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bioRxiv - Plant Biology 2024Quote: ... 4 DAT seedlings were treated with 1 M PEP1 for 24h before they were imaged using confocal miscrscopes (Leica TCS SP8 and Zeiss LSM700). To visualize the GFP protein ...
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bioRxiv - Neuroscience 2024Quote: ... following imaging and either underwent cardiac perfusion with phosphate buffered saline and 4% paraformaldehyde or had their brains extracted and post-fixed in 4% paraformaldehyde before being cut on a vibratome in 40 µm sections (Leica VT1000S, Deer Park, IL). Sections through the olfactory bulb were mounted on slides and were coverslipped using Fluoromount-G containing DAPI (SouthernBiotech ...
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bioRxiv - Neuroscience 2024Quote: ... They were then perfused transcardially with ice-cold (4°C) phosphate buffered saline (PBS) using the Perfusion Two™ system (Leica Biosystems, Chicago, IL).
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bioRxiv - Biochemistry 2022Quote: ... Neutrophils were then counted at the site of transection at 2 hours post injury (hpi) using a fluorescence dissecting stereomicroscope (Leica).
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bioRxiv - Physiology 2021Quote: Immunohistochemical staining in pancreas and fat sections was carried out on 1-2 μm section cut from paraffin blocks in an automated immunostainer from Leica biosystems (BOND RX ...
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bioRxiv - Developmental Biology 2021Quote: ... Immunofluorescent analysis and imaging were performed on a Leica SP5X Upright 2-Photon Confocal microscope using LAS AF software (Leica) and a Leica 63X (type ...
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bioRxiv - Plant Biology 2021Quote: ... The cross section of 2-4μm was cut with a glass knife using the ultramicrotome Leica EM UC7 (Leica Microsystems). The semi-thin sections were stained for 30mins in 0.1% aniline blue in 0.1M K3PO4 at RT ...
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bioRxiv - Plant Biology 2021Quote: ... The stained sections were observed within 1hr to detect callose deposition using confocal laser scanning microscopy (TCS SP5 2; Leica).
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bioRxiv - Neuroscience 2020Quote: ... Spinal cords were removed from the spinal column by hydraulic extrusion and embedded in low-melting point agarose (2-3%) before sectioning using a vibratome (Leica) into 300 µm thick coronal slices in ice-cold oxygenated ACSF ...
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bioRxiv - Neuroscience 2022Quote: ... brains were flash frozen in 2-methylbutane and stored at −20°C until sectioning at 40μm on a cryostat (Leica Biosystems) into cryoprotectant.
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bioRxiv - Immunology 2022Quote: Staining for SARS-CoV-2 antigen was achieved on the Bond RX automated system with the Polymer Define Detection System (Leica) used per manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2021Quote: ... The tissues were merged in 10% and 30% sucrose-PBS solutions at RT (2 h each solution) followed by 1:1 solution 30% sucrose:tissue-freezing medium (Leica 14020108926). Afterwards ...
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bioRxiv - Physiology 2021Quote: ... snap-frozen in 2-methylbutane on dry ice and sectioned into 10 μm slices using CryoJane Tape-Transfer System (Leica). Masson’s trichrome stain for cardiac fibrosis was performed according to the manufacturer’s instructions (Sigma) ...
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bioRxiv - Neuroscience 2022Quote: ... Thin sections from the area of 2-photon imaging were cut with a diamond knife on an EM UC6 ultramicrotome (Leica), collected on pioloform-coated copper Synaptek slot grids (Electron Microscopy Sciences) ...
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bioRxiv - Microbiology 2022Quote: ... Approximately 2 µL of the spore suspension per sample was then processed with Leica EM ICE HPF apparatus (Leica Microsystems), and then freeze substituted and embedded in Lowicryl HM20 (Electron Microscopy Sciences ...
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bioRxiv - Plant Biology 2022Quote: ... We cut the Spurr’s blocks into semi-thin sections (0.75–2 μm) with glass knives on an ultramicrotome Leica Ultracut UCT (Leica Microsystems) stained with a solution containing 1% (w/v ...
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bioRxiv - Cell Biology 2020Quote: ... Images were captured using Metamorph software at every 15 minutes in 37C (PeCon Tempcontrol 37-2) using confocal spinning disk system (Leica DMI 6000 B equipped with YOKOGAWA CSU-W1 Confocal Scanner Unit ...
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bioRxiv - Neuroscience 2021Quote: ... embedded in 2% LTE agarose/ Tris-buffered saline (TBS) and cut coronally in 50 µm sections on a vibratome (VT 1000S, Leica). Sections underwent antigen retrieval in a solution of 0.1 M sodium citrate (pH 6) ...
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bioRxiv - Immunology 2021Quote: ... Sections were serially imaged over the course of 1-2 hours and compiled using LAS X software (Leica Microsystems, USA). Image analysis was performed using Imaris software (Bitplane ...
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bioRxiv - Developmental Biology 2021Quote: ... The tissues were then cryoprotected in 10% and 30% sucrose solutions for 2 hrs at RT and finally incubated in 30% sucrose and tissue embedding medium (Leica) (1:1 ...
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bioRxiv - Developmental Biology 2020Quote: ... 25% laser power (Pnmy-2nmy-2::gfp) or 13% laser power (Ppie-1mCherry::phPLCδ) was used (Leica TCS SP5-II). All analyses were performed on center Z-slices of Z-stacks through the NSMnb ...
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bioRxiv - Immunology 2021Quote: ... After fixation organs were stored in PBS till paraffin embedment and cut of serial sections on a microtome (2-3 µm; Leica RM 2035 ...
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bioRxiv - Neuroscience 2022Quote: ... 2-photon imaging was performed using an upright microscope optimized for confocal and multiphoton microscopy (TCS SP5 II, Leica Microsystems). After the mouse was mounted to a stereotaxic holder (SR-AM and STS-A ...
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bioRxiv - Cancer Biology 2022Quote: ... with antigen retrieval at 100ºC for 20 min with Epitope Retrieval Solution 1 or 2 respectively (AR9961, AR9640, Leica Biosystems). TMAs were blind-scored by multiplying staining intensity with staining coverage ...
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bioRxiv - Cancer Biology 2022Quote: ... Samples and probes were co-denatured at 75°C for 2 min followed by incubation at 37°C overnight in a humidified chamber using ThermoBrite System (Leica). Slides were then washed for 2 min in 0.4x SSC at 75°C followed by a 30 second wash in 2x SSC at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... Grids were post-stained for 30 min with 2% uranyl acetate (Roth) and 20 min with 3% lead citrate (Leica). TEM images were acquired using a Zeiss Leo 912 Omega (Zeiss ...
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bioRxiv - Neuroscience 2022Quote: ... the brain was cut into 2 mm thick coronal sections in 0.15 M cacodylate buffer using a vibratome (Leica VT1200). Then ...
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bioRxiv - Bioengineering 2022Quote: ... a ~ 2 nm layer of gold coating was completed using a sputtering machine (Leica EM ACE 600, Buffalo Grove, IL) and was imaged using an SEM (Apreo 2 ...
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bioRxiv - Systems Biology 2024Quote: ... Dried smears were incubated in 70% ethanol for 2 hours and hematoxylin/eosin (H&E) stained using an automatic Leica Multistainer and Coverslipper (Leica ST5020+CV5030 ...
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The transcriptomic landscape of monosomy X (45,X) during early human fetal and placental developmentbioRxiv - Genetics 2024Quote: ... IHC for CSF2RA (Colony Stimulating Factor 2 Receptor Subunit Alpha) was undertaken on 3µm sections using a Leica Bond-max automated platform (Leica Biosystems). In brief ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The growth culture medium was changed every 2-3 days and cell morphology was regularly checked by visual inspection with phase-contrast microscopy (Leica DMIC ...
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bioRxiv - Neuroscience 2023Quote: ... brains tissue fixed in 4% paraformaldehyde were cryoprotected in 30% sucrose for 2 hours followed by embedding in OCT for sectioning to 10μm thickness on a cryostat (Leica CM1860). Prior to staining ...
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bioRxiv - Immunology 2023Quote: ... as described previously.23 All heat induced epitope retrievals were performed with Bond™ Epitope Retrieval 2 (AR9640, Leica Biosystems) for 20 minutes at 95°C ...
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bioRxiv - Genetics 2023Quote: ... the worms were mounted on a 2% agarose pad and images were captured using a Leica compound microscope (Leica DM5000B).
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bioRxiv - Neuroscience 2023Quote: ... were imaged using a 0.75 NA 20X objective at 2 μm depth intervals on a Leica TCS SP8 laser scanning confocal microscope (Leica Microsystems). For the validation of the mouse line (assay # 1) ...
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bioRxiv - Cancer Biology 2023Quote: ... Non-consecutive sections were cut with a thickness of 10μm and placed onto 2 capture areas of 10X Visium Spatial Gene expression slide using a cryostat (Leica CM3050S). The tissue Optimization kit was used to determine permeabilization times (24 min) ...
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bioRxiv - Cell Biology 2022Quote: ... Embryos were injected intravascularly in the duct of Cuvier with 27,6 nL of Membright Cy5-labeled tEVs or 4T1CD63pHluorinmScarlet-tEVs (at 10E10 EVs/ml) with a Nanoject microinjector 2 (Drummond) under a M205 FA stereomicroscope (Leica) as described previously (Mary et al. ...
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bioRxiv - Plant Biology 2023Quote: ... leaving around 2 mm of the root outside the needle which was submerged in a tissue-freezing medium (Jung, Leica) and quickly frozen in liquid nitrogen (Vogel-Mikuš et al. ...
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bioRxiv - Neuroscience 2023Quote: ... five µm section were cut followed by a Heat-Induced Epitope Retrieval at 100°C during 20 min in BOND Epitope Retrieval 2 (ER2) solution (Leica) with 0.1 Proteinase K before Deparaffinizing ...
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bioRxiv - Neuroscience 2023Quote: ... the animals were placed under 0.8-1% isoflurane anesthesia (2% used for induction) and imaged on an upright laser-scanning confocal (Leica SP8) or upright laser-scanning two-photon microscope (Bruker Ultima ...
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bioRxiv - Neuroscience 2023Quote: ... The caudal corpus callosa alongside adjacent tissue were punched with a 2 mm diameter punching tool and embedded in Epon (EM TP, Leica) prior to conventional embedding steps ...
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bioRxiv - Developmental Biology 2023Quote: ... dehydrated in methanol and cleared in Benzyl Alcohol/Benzyl Benzoate (1/2) prior to mounting and imaging with a SP8X confocal microscope (Leica). A 3D binary mask was built using Fiji to obtain a binary image of the tissue ...
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bioRxiv - Microbiology 2024Quote: ... the eYFP fluorescence of fusion proteins was analyzed 2–3 days after infiltration using a confocal microscope (Leica TCS SP8).
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bioRxiv - Developmental Biology 2024Quote: ... Slides were baked and dewaxed on Leica Bond-RX and pretreated with Bond Epitope Retrieval Solution 2 (ER2) (AR9640) from Leica at 100°C for 40 minutes ...
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bioRxiv - Developmental Biology 2024Quote: Immunofluorescent analysis and imaging were performed on a Leica SP5X Upright 2-Photon Confocal microscope using LAS AF software (Leica) and a Leica 63X (type ...
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bioRxiv - Neuroscience 2024Quote: Confocal acquisitions were performed using a Leica SP8 White Light Laser 2 on an inverted stand (Leica Microsystems, Mannheim, Germany) and with a x20 (NA 0.75 ...
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bioRxiv - Neuroscience 2024Quote: ... the hemisphere was gelatine-embedded and coronally sectioned between anteroposterior coordinates -2 mm and -4.8 mm (dorsal hippocampus) with a cryostat (Leica CM3050 S, Leica Biosystems). Sections of 40 µM were placed on anti-freeze solution [30% glycerol ...