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Citations for Leica :
851 - 900 of 1409 citations for 2 1H Imidazol 1 yl 6 methyl 4 pyrimidinemethanamine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... were imaged using a 0.75 NA 20X objective at 2 μm depth intervals on a Leica TCS SP8 laser scanning confocal microscope (Leica Microsystems). For the validation of the mouse line (assay # 1) ...
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bioRxiv - Cancer Biology 2023Quote: ... Non-consecutive sections were cut with a thickness of 10μm and placed onto 2 capture areas of 10X Visium Spatial Gene expression slide using a cryostat (Leica CM3050S). The tissue Optimization kit was used to determine permeabilization times (24 min) ...
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bioRxiv - Cell Biology 2022Quote: ... Embryos were injected intravascularly in the duct of Cuvier with 27,6 nL of Membright Cy5-labeled tEVs or 4T1CD63pHluorinmScarlet-tEVs (at 10E10 EVs/ml) with a Nanoject microinjector 2 (Drummond) under a M205 FA stereomicroscope (Leica) as described previously (Mary et al. ...
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bioRxiv - Developmental Biology 2024Quote: Immunofluorescent analysis and imaging were performed on a Leica SP5X Upright 2-Photon Confocal microscope using LAS AF software (Leica) and a Leica 63X (type ...
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bioRxiv - Cell Biology 2024Quote: ... or 2 hours of 200nM BafA1 before immediate cryo-fixation by high pressure freezing (Leica EM ICE High Pressure Freezer), with 20% BSA in DMEM as a cryoprotectant ...
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bioRxiv - Developmental Biology 2024Quote: ... Slides were baked and dewaxed on Leica Bond-RX and pretreated with Bond Epitope Retrieval Solution 2 (ER2) (AR9640) from Leica at 100°C for 40 minutes ...
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bioRxiv - Neuroscience 2024Quote: ... They were then embedded in 2% agarose and sliced into coronal sections (70-100µm thick) using a Vibratome (Leica Microsystems). The slices were washed in 0.01M PBS ...
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bioRxiv - Neuroscience 2024Quote: Confocal acquisitions were performed using a Leica SP8 White Light Laser 2 on an inverted stand (Leica Microsystems, Mannheim, Germany) and with a x20 (NA 0.75 ...
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bioRxiv - Neuroscience 2024Quote: ... frozen at -80°C for 30 minute then at -20°C for 2 hours before being sectioned using a cryostat (Leica, 50 µm thick coronal sections for immunocytochemistry) ...
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bioRxiv - Microbiology 2024Quote: ... the eYFP fluorescence of fusion proteins was analyzed 2–3 days after infiltration using a confocal microscope (Leica TCS SP8).
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bioRxiv - Neuroscience 2024Quote: ... the hemisphere was gelatine-embedded and coronally sectioned between anteroposterior coordinates -2 mm and -4.8 mm (dorsal hippocampus) with a cryostat (Leica CM3050 S, Leica Biosystems). Sections of 40 µM were placed on anti-freeze solution [30% glycerol ...
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bioRxiv - Cell Biology 2024Quote: ... samples were freeze-substituted at -90°C in anhydrous acetone containing 2% osmium tetroxide and 0.1% uranyl acetate at -90°C for 72 hours and warmed to room temperature with a gradient of +5°C per hour (EM AFS-2, Leica). Sections of the samples were analysed in a Tecnai Spirit T12 Electron microscope equipped with an Eagle OCD camera (Thermo Fisher Scientific).
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bioRxiv - Evolutionary Biology 2024Quote: ... embryos were introduced into an M9 droplet on a 2% agarose pad and immediately imaged on a Leica SP8 confocal on a DM6000 upright microscope (Leica LAS X image acquisition software ...
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bioRxiv - Neuroscience 2021Quote: ... The brains were transferred and kept in 30% sucrose in PBS for more than 48 h at 4 °C and then sectioned using a freezing microtome (SM2010R, Leica; EF-13, Nihon Microtome Laboratory) at a thickness of 40 μm parallel to the coronal plane ...
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bioRxiv - Neuroscience 2020Quote: ... the rats were sacrificed via transcardiac perfusion with 4% paraformaldehyde (PFA) using the Perfusion Two™ Automated Pressure Perfusion System (Leica Biosystems, Buffalo Grove, IL) (n = 12 ...
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bioRxiv - Cancer Biology 2020Quote: ... was performed on 4-μm FFPE sections arranged on the above mentioned TMA using commercial equipment (Leica Bond RXm System; Leica Microsystems Ltd, Milton Keynes, UK) and a detection kit (Bond Polymer Refine Red Detection Kit ...
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bioRxiv - Neuroscience 2021Quote: The whole-mount samples were embedded in gelatin/albumin with 4% of Glutaraldehyde and sectioned at 20 μm thickness on a vibratome (Leica, VT1000 S vibrating blade microtome). The sections were mounted in Fluoromount Aqueous Mounting Medium (Sigma ...
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bioRxiv - Neuroscience 2023Quote: ... Brains were then fixed in 4% PFA overnight before sectioning 50 µm coronal slices using a Leica VT1000S vibratome (Leica Biosystems, Deer Park, IL, USA).
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bioRxiv - Neuroscience 2024Quote: ... Brains were post-fixed with PFA 4% overnight and 50 µm thick coronal sections were cut with a VT 1000S vibratome (Leica Microsystems Inc., Buffalo Grove, IL). Free-floating vibratome sections were processed as follows ...
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bioRxiv - Pathology 2022Quote: ... and incubated with a 1:200 dilution of anti-4-HNE and anti-AKR1A1 primary antibody overnight at 4°C primary antibody along with the Novolink™ Max Polymer Detection System (Leica Biosystems, Wetzlar, Hesse, Germany) and DAB substrate (3,30-diaminobenzidine ...
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bioRxiv - Neuroscience 2023Quote: ... Post-fixed brains (PFA 4%) were coronally sectioned at either 30 μm (IHC) with a cryostat (Leica CM 1950, Leica Biosystems, Deer Park, IL, USA). Mice left tibia was dissected and placed in 4% PFA then follow 2.10 Bone histology analysis method.
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bioRxiv - Biochemistry 2024Quote: ... The excess liquid was blotted for 4 s prior to plunge-freezing into liquid ethane using a Leica EM GP2 plunger (Leica Microsystems; 95% humidity, 14 °C). Grids were stored in liquid nitrogen prior to data collection ...
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bioRxiv - Cancer Biology 2021Quote: ... CD4 (1:100, HIER 1, mouse monoclonal, clone 4B12, Leica, Germany; CD4-368-L-CE-H), CD8 (1:50 ...
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bioRxiv - Cancer Biology 2021Quote: ... and p53 (D07, 1:50, Leica) were applied with negative controls as previously described2.
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bioRxiv - Genomics 2021Quote: ... mouse Ki67 (1:1000, Leica, ACK02), rabbit Lyve1 (1:200 ...
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bioRxiv - Cancer Biology 2022Quote: ... ER Solution 1 (AR9961, Leica Biosystems) was used ...
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bioRxiv - Cell Biology 2024Quote: ... Emerin (Leica Emerin-NCL, 1:1000). DAPI was added 1:1000 in PBS for 15 minutes ...
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bioRxiv - Cancer Biology 2024Quote: ... p53 (CM5, 1:1000 Leica Novocastra), p21 (EPR18021 ...
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bioRxiv - Cell Biology 2020Quote: ... Dried samples were then rotary-shadowed with 2 nm of platinum and 5-8 nm of carbon using an ACE600 high vacuum metal coater (Leica Microsystems). Platinum replicas were floated off the glass by 5% hydrofluoric acid ...
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bioRxiv - Cell Biology 2020Quote: ... cells were loaded with fura-2/AM in the dark (5 μM; 30 min; RT; S1) and imaged with an upright microscope (Leica DMI6000FS, Leica Microsystems) equipped for ratiometric live-cell imaging with a 150W xenon arc lamp ...
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bioRxiv - Cell Biology 2020Quote: ... The loosely packed cells in BSA were high-pressure frozen in a Leica Empact 2 high-pressure freezer (Leica, Vienna, Au), and submitted to a quick freeze-substitution protocol that preserves fluorescence (35 ...
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bioRxiv - Bioengineering 2021Quote: ... gravid day 1 adults animals were picked onto prepared palmitic acid plates and plates were imaged at 2 and 5 hours at 1.6x on a stereomicroscope (Leica M165 FC) using a 1.3 MP CMOS camera (Thorlabs DCC1645C ...
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bioRxiv - Physiology 2021Quote: ... Slides were prepared using the frozen slide delay prior to pre-treatments using Epitope Retrieval Solution 2 (Cat No. AR9640, Leica Biosystems) at 95°C for 5 min ...
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bioRxiv - Biophysics 2021Quote: ... Then 3 μL of concentrated hTg were dispensed to the hydrophilic surface of the grid prior to single side blotting for 2 s and plunge freezing in liquid ethane using a Leica EM GP2 plunger (Leica Microsystems) operating at 20 °C and 80% relative humidity.
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bioRxiv - Evolutionary Biology 2022Quote: ... The fixed samples were embedded in Technovit 8100 (Kulzer, Germany) and processed into 2 μm tissue sections using a rotary microtome RM2255 (Leica, Germany). The AlexaFluor555-labeled oligonucleotide probes Eco934 (5’-CAT GCT CCA CCG CTT GTG-3’ ...
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bioRxiv - Physiology 2021Quote: ... Images were acquired at a rate of 50 Hz with a wide-field fluorescence microscope (DMIRE-2, Leica Microsystems, Wetzlar, Germany) as previously described [24].
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bioRxiv - Immunology 2020Quote: ... The uptake of the fluorescently labeled SVV in IBRS-2 and 293T cells was visualized with a fluorescence microscope (Leica, Germany).
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bioRxiv - Developmental Biology 2020Quote: ... Wild-type 1 dpf larvae were positioned on top of a 2% agarose plate and imaged using a Leica M165FC dissection scope (Leica Microsystems) with a SPOT RT3 camera (SPOT Imaging) ...
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bioRxiv - Bioengineering 2021Quote: ... the impact velocity was 6.0 m/s and the depth of cortical deformation was 2 mm with 100 ms impact duration (Impact ONE; Leica Microsystems). After the impact ...
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bioRxiv - Plant Biology 2020Quote: Confocal laser scanning microscopy of ovules stained with SR2200 and TO-PRO-3 iodide was performed on an upright Leica TCS SP8 X WLL2 HyVolution 2 (Leica Microsystems) equipped with GaAsP (HyD ...
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bioRxiv - Plant Biology 2020Quote: ... mesophyll cells of 2-week-old transgenic Arabidopsis plants expressing Raf36-GFP were observed with a confocal microscope SP8X (Leica Microsystems) with the time-gating method (Kodama ...
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bioRxiv - Neuroscience 2020Quote: ... Coronal slices (300 µm thick) containing visual cortex were prepared using a vibrating blade microtome (Leica VT1200S or Campden 7000smz-2). Slices were immediately transferred to a submerged holding chamber with regular ACSF containing (in mM) ...
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bioRxiv - Plant Biology 2021Quote: Confocal laser scanning microscopy of ovules stained with SR2200 and TO-PRO-3 iodide was performed on an upright Leica TCS SP8 X WLL2 HyVolution 2 (Leica Microsystems) equipped with GaAsP (HyD ...
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bioRxiv - Immunology 2021Quote: ... Ultra-thin (50 nm to 60 nm) sections were sectioned with a diamond knife (Jumdi, 2 mm) on an ultramicrotome (Leica Ultracut), contrasted with 2% uranyl acetate (Polyscience ...
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bioRxiv - Neuroscience 2023Quote: ... The brain was let chill for ∼2 min before slicing in ice-cold sucrose-ACSF using a vibratome (Leica VT1000S, Leica, Germany). Brain slices were immediately transferred to a submerged holding chamber containing room-temperature holding ACSF composed of (mM) ...
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bioRxiv - Neuroscience 2023Quote: ... The brain was let chill for ∼2 min before slicing in ice-cold sucrose-ACSF using a vibratome (Leica VT1000S, Leica, Germany). Brain slices were immediately transferred to a submerged holding chamber containing room-temperature holding ACSF composed of (mM) ...
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bioRxiv - Developmental Biology 2023Quote: ... GFP images were obtained every 2 s with a x40 oil-immersive objective (HC PL FLUOTAR 40x/1.30 OIL 340 nm, Leica Microsystems CMS) used for CBs and a x10 objective (N PLAN 10x/0.25 achromatic corrected objective ...
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bioRxiv - Molecular Biology 2023Quote: ... The samples were waxed in liquid paraffin for 2 h and embedded to make paraffin blocks using a tissue embedder (EG1150, Leica, Germany). Four-micron sections were cut by paraffin slicer (RM2245 ...
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bioRxiv - Cell Biology 2022Quote: ... 40 s) Quantifoil Cu 300 R2/2 grid and plunge frozen in liquid ethane with a GP2 (Leica Microsystems, Wetzlar, Germany) blotted from behind ...
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bioRxiv - Neuroscience 2024Quote: Mice were anesthetized using vaporized isoflurane (5% for induction, 1.5% for maintenance) and placed in a stereotactic frame (Leica, Angle 2). Opthalmic ointment was applied and rectal temperature and respiration rate were monitored throughout surgery ...