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Citations for Leica :
851 - 900 of 1328 citations for 1 6 Chloropyridazin 3 yl piperidine 4 carboxamide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2020Quote: ... The animals were given cold shock for 2 minutes at -4°C and then photographed along with a reference scale under a stereo zoom microscope (Leica DFC 290, Leica Microsystems GmbH, Wetzlar, Germany) with a mounted digital camera ...
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bioRxiv - Neuroscience 2022Quote: ... Mice were head-fixed in a 1.5 x 4-inch polycarbonate tube under a custom-built fluorescence macroscope consisting of back-to-back 50 mm objective lens (Leica, 0.63x and 1x magnification), separated by a 495nm dichroic mirror (Semrock Inc ...
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bioRxiv - Molecular Biology 2020Quote: Frozen coronal ovine hypothalamic blocks (n=4 per group) for in-situ hybridization were cut into 16µm sections using a cryostat (CM3050s Leica Microsystems, Ltd., Milton Keynes, UK), and thaw mounted onto poly-l-lysine coated slides (VWR International ...
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bioRxiv - Neuroscience 2021Quote: ... and sacrificed via transcardiac perfusion with 10% sucrose (isotonic at 9.25% in distilled water; prewash) and 4% paraformaldehyde (PFA; fixative) using the Perfusion Two™ Automated Pressure Perfusion System (Leica Biosystems, Buffalo Grove, IL) (n = 12 ...
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bioRxiv - Neuroscience 2022Quote: ... embedded them in 4% w/v agar/PBS and prepared 40 µm thick slices using a vibrating blade microtome (VT1200S, Leica Microsystems, Buffalo Grove, IL). The slices were post-fixed in 4% PFA/PBS for 30 min at room temperature (RT ...
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bioRxiv - Neuroscience 2022Quote: ... 0.4 mM L-ascorbic acid and the pH was adjusted to 7.4) and then sliced on a vibratome (Leica, VT1200 S, Deerfield, IL, USA). Brain slices (300 μm thick ...
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bioRxiv - Developmental Biology 2024Quote: ... Sections were mounted with Fluorsave (Table 1S) and stored in the dark at 4°C until imaging on a Leica Stellaris5 confocal microscope (Leica Microsystems CMS GmbH, Germany). Quantification was performed as previously described42 ...
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bioRxiv - Biochemistry 2023Quote: ... the grids were flash-frozen in liquid ethane at ∼90 % humidity and 4 °C with a Leica EM GP rapid-plunging device (Leica, Buffalo Grove, IL, USA) before being transferred into liquid nitrogen for storage ...
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bioRxiv - Cancer Biology 2024Quote: Cells were seeded on glass bottom dishes then image acquisitions were performed every 2 min for 4 using LiveSR spinning-disk microscope (Leica, Leica Microsystems GmbH, Wetzlar, Germany) with environmental control at 37°C and CO2 provider ...
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bioRxiv - Neuroscience 2024Quote: ... They were then perfused transcardially with ice-cold (4°C) phosphate buffered saline (PBS) using the Perfusion Two™ system (Leica Biosystems, Chicago, IL).
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bioRxiv - Neuroscience 2024Quote: ... following imaging and either underwent cardiac perfusion with phosphate buffered saline and 4% paraformaldehyde or had their brains extracted and post-fixed in 4% paraformaldehyde before being cut on a vibratome in 40 µm sections (Leica VT1000S, Deer Park, IL). Sections through the olfactory bulb were mounted on slides and were coverslipped using Fluoromount-G containing DAPI (SouthernBiotech ...
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bioRxiv - Neuroscience 2024Quote: ... Sagittal 300-μm-thick slices were cut from the dorsal level of the hippocampus48 at 2-4 mm from midline using a vibratome (Leica VT-1200, Nussloch, Germany). Slices were stored in a reserve chamber containing slicing solution at 34°C for at least 30 min and subsequently at room temperature (20 – 25°C).
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bioRxiv - Cancer Biology 2021Quote: ... CD20 (1:100, HIER 1, mouse monoclonal, clone L26, Leica, Germany; CD20-L26-L-CE), CD163 (1:500 ...
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bioRxiv - Cancer Biology 2021Quote: ... CD8 (1:50, HIER 1, mouse monoclonal, clone 4B11, Leica, Germany, CD8-4B11-L-CE), CD20 (1:100 ...
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bioRxiv - Plant Biology 2023Quote: ... 100% ethanol) and incubated overnight in a 1:1 solution of 100% ethanol:Historesin solution (Leica). The solution was exchanged with Historesin and the samples incubated again overnight at 4°C ...
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bioRxiv - Systems Biology 2023Quote: ... in a sequential order as shown: 1) Opal 480/CD71 (1:80, 10F11, Leica concentrate), 2 ...
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bioRxiv - Neuroscience 2021Quote: ... The brains were transferred and kept in 30% sucrose in PBS for more than 48 h at 4 °C and then sectioned using a freezing microtome (SM2010R, Leica; EF-13, Nihon Microtome Laboratory) at a thickness of 40 μm parallel to the coronal plane ...
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bioRxiv - Neuroscience 2020Quote: ... the rats were sacrificed via transcardiac perfusion with 4% paraformaldehyde (PFA) using the Perfusion Two™ Automated Pressure Perfusion System (Leica Biosystems, Buffalo Grove, IL) (n = 12 ...
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bioRxiv - Cancer Biology 2020Quote: ... was performed on 4-μm FFPE sections arranged on the above mentioned TMA using commercial equipment (Leica Bond RXm System; Leica Microsystems Ltd, Milton Keynes, UK) and a detection kit (Bond Polymer Refine Red Detection Kit ...
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bioRxiv - Neuroscience 2021Quote: The whole-mount samples were embedded in gelatin/albumin with 4% of Glutaraldehyde and sectioned at 20 μm thickness on a vibratome (Leica, VT1000 S vibrating blade microtome). The sections were mounted in Fluoromount Aqueous Mounting Medium (Sigma ...
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bioRxiv - Neuroscience 2023Quote: ... Brains were then fixed in 4% PFA overnight before sectioning 50 µm coronal slices using a Leica VT1000S vibratome (Leica Biosystems, Deer Park, IL, USA).
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bioRxiv - Neuroscience 2024Quote: ... Brains were post-fixed with PFA 4% overnight and 50 µm thick coronal sections were cut with a VT 1000S vibratome (Leica Microsystems Inc., Buffalo Grove, IL). Free-floating vibratome sections were processed as follows ...
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bioRxiv - Pathology 2022Quote: ... and incubated with a 1:200 dilution of anti-4-HNE and anti-AKR1A1 primary antibody overnight at 4°C primary antibody along with the Novolink™ Max Polymer Detection System (Leica Biosystems, Wetzlar, Hesse, Germany) and DAB substrate (3,30-diaminobenzidine ...
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bioRxiv - Neuroscience 2023Quote: ... Post-fixed brains (PFA 4%) were coronally sectioned at either 30 μm (IHC) with a cryostat (Leica CM 1950, Leica Biosystems, Deer Park, IL, USA). Mice left tibia was dissected and placed in 4% PFA then follow 2.10 Bone histology analysis method.
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bioRxiv - Biochemistry 2024Quote: ... The excess liquid was blotted for 4 s prior to plunge-freezing into liquid ethane using a Leica EM GP2 plunger (Leica Microsystems; 95% humidity, 14 °C). Grids were stored in liquid nitrogen prior to data collection ...
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bioRxiv - Cancer Biology 2021Quote: ... CD4 (1:100, HIER 1, mouse monoclonal, clone 4B12, Leica, Germany; CD4-368-L-CE-H), CD8 (1:50 ...
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bioRxiv - Cancer Biology 2021Quote: ... and p53 (D07, 1:50, Leica) were applied with negative controls as previously described2.
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bioRxiv - Genomics 2021Quote: ... mouse Ki67 (1:1000, Leica, ACK02), rabbit Lyve1 (1:200 ...
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bioRxiv - Cancer Biology 2022Quote: ... ER Solution 1 (AR9961, Leica Biosystems) was used ...
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bioRxiv - Cell Biology 2024Quote: ... Emerin (Leica Emerin-NCL, 1:1000). DAPI was added 1:1000 in PBS for 15 minutes ...
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bioRxiv - Cancer Biology 2024Quote: ... p53 (CM5, 1:1000 Leica Novocastra), p21 (EPR18021 ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were then moved to 4°C for 5 min and immediately imaged using a confocal microscope (Leica SPX5 with a 63X 1.4 N.A. oil objective). Internalization of antigen was quantified by determining the percentages of cells with intracellularly-located AF488-Fab’ goat-anti-mouse IgM+G puncta in each field and by measuring the AF488 FI associated with intracellular puncta in individual cells ...
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bioRxiv - Cancer Biology 2022Quote: ... Slides were stained manually with mouse anti-TTF1 (clone SPT24; TTF-1-L-CE, Leica, 1/400, 25’) and mouse anti-P40 (clone BC28 ...
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bioRxiv - Cancer Biology 2021Quote: ... CD3 (clone LN10, dilution 1:500, Leica), CD14 (clone SP192 ...
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bioRxiv - Neuroscience 2021Quote: ... anti-GFAP (mouse, GA5, Leica, 1:200), anti-n-terminal APP (22c11 ...
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bioRxiv - Cell Biology 2021Quote: ... for 1 hour and visualised by Leica Sp8 inverted confocal microscopy in serial sections of 20 μm ...
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bioRxiv - Cell Biology 2024Quote: ... eMyHC (Leica NCL-MHC-d; 1:200) for staining of muscle cryosections ...
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bioRxiv - Developmental Biology 2023Quote: ... rabbit anti-p53 (1:1000; Leica Biosystems), rabbit-anti-active caspase-3 (1:1000 ...
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bioRxiv - Cell Biology 2024Quote: ... Epitope Retrieval Solution 1 (Leica Cat#AR9961), Epitope Retrieval Solution 2 (Leica Cat#AR9640) ...
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bioRxiv - Developmental Biology 2022Quote: ... mouse anti-Ki67 (1:500, Novocastra/Leica), rabbit anti-PH3 (1:1000 ...
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bioRxiv - Molecular Biology 2022Quote: ... eMyHC (Leica NCL-MHC-d;1:200), laminin (Sigma-Aldrich L9393 ...
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bioRxiv - Developmental Biology 2021Quote: ... Then the samples were embedded in a mixture of OCT and water at a ratio of 1:1 and sectioned at 10 μm thickness with freezing microtome (Leica). The mRNA detection by in situ hybridization was performed as previously described (Lauter et al. ...
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bioRxiv - Neuroscience 2021Quote: ... the tissue was trimmed to less than 1 × 1 mm and one side was exposed using an ultramicrotome (UC7, Leica), then turned downwards to be remounted to a metal pin with conductive silver epoxy (CircuitWorks ...
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bioRxiv - Developmental Biology 2021Quote: ... The tissues were merged in 10% and 30% sucrose-PBS solutions at RT (2 h each solution) followed by 1:1 solution 30% sucrose:tissue-freezing medium (Leica 14020108926). Afterwards ...
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bioRxiv - Neuroscience 2021Quote: ... microglial density quantifications and Iba-1:P2RY12 or Iba-1:TMEM119 colocalization analysis 40 µm free-floating sections were cut with a sliding microtome (Leica) and stained with relevant antibodies in 10% normal goat serum (NGS ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... The gonadal tissues for histology were dehydrated in a tissue processor (TP 1020-1-1, Leica Microsystems Nussloch GmbH, Germany) and thereafter embedded in paraffin ...
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bioRxiv - Genomics 2023Quote: ... slides were deparaffinized and automatically stained with specific antibodies against synovial cell markers (PECAM-1, DAKO JC/70a, 1:10; CD20, Ventana Roche L26, prediluted; CD3, Leica LN10 ...
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bioRxiv - Neuroscience 2022Quote: ... Sakura Finetek) and 30% sucrose–PBS (1:1) for cryosectioning (25 μm-thick sections) using a Leica Cryostat (Leica, CM1860). For immunofluorescence staining ...
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bioRxiv - Cancer Biology 2020Quote: ... was diluted 1:100 in Antibody Diluent (Leica).
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bioRxiv - Microbiology 2021Quote: ... SARS-CoV-2 Nucleoprotein: 1:2000 (Leica, AR9961) 30minutes ...